Genetic variability of the short-haired and rough-haired Segugio Italiano dog breeds and their genetic distance from the other related Segugio breeds

Four samples of Neoplecostomus yapo were analyzed through the allozyme electrophoresis technique in corn starch gel. The allozyme pattern was similar to those found in N. paranensis with 24 loci scored. Two samples (ribeirão Atlântico and ribeirão Uraí) showed monomorphic bands for all 24 loci, whereas the other two (rio Verde and rio Fortaleza) showed 8.3% of polymorphic loci. The He genetic variability estimates for the rios Verde and Fortaleza populations were 0.0195 and 0.0179, respectively, too much inferior to the mean heterozygosity summed to species from the whole world (0.051). The Wright statistical values F IS = 0.5181, F IT = 0.5681 and F ST = 0.1039 and the genetic distance of Nei values showed that the four samples are genetically very similar to each other and that there is homozygote excess in the polymorphic loci.

Download Full-text

ALLOZYME DIVERSITY IN CHESTNUT (CASTANEA SPP.).

HortScience ◽

10.21273/hortsci.29.7.736a ◽

1994 ◽

Vol 29 (7) ◽

pp. 736a-736

Author(s):

Hongwen Huang ◽

Fenny Dane ◽

Joseph Norton

Keyword(s):

Genetic Variability ◽

Genetic Distance ◽

The Other ◽

Changjiang River ◽

Allozyme Diversity ◽

Allele Distribution ◽

Polymorphic Loci ◽

The Changjiang River ◽

Enzyme Systems ◽

River Region

Allozyme genetic variability in three chestnut (Castanea) species was investigated using 19 loci from ten enzyme systems. G-tests of heterogeneity of isozymic allele distribution showed significant differences in both intraspecific and interspecific populations. C. mollissima was found to possess a significantly higher value of mean gene heterozygosity (H=0.3050±0.0419), percentage of polymorphic loci (P=84.21%) and average number of alleles per locus (A=2.05) than any other species in the Castanea section Eucustanon. When the genetic variability of populations of C. mollissimo from four regions in China was investigated, population from the Changjiang river region showed a markedly higher mean gene heterozygosity (H=0.3480±0.0436) than populations from the other regions. An approximately identical genetic distance between the population from the Changjiang river region and populations from three other regions was observed, while populations from the latter regions showed almost the same genetic distance from each other.

Download Full-text

Genetic analysis of ten sheep breeds using microsatellite markers

Canadian Journal of Animal Science ◽

10.4141/a99-086 ◽

2000 ◽

Vol 80 (1) ◽

pp. 9-17 ◽

Cited By ~ 17

Author(s):

A. Farid ◽

E. O'Reilly ◽

C. Dollard ◽

C.R. Kelsey Jr.

Keyword(s):

North America ◽

Genetic Variability ◽

Genetic Analysis ◽

Genetic Distance ◽

Microsatellite Markers ◽

Microsatellite Loci ◽

Narrow Range ◽

The Other ◽

The North ◽

Expected Heterozygosity

The genetic variability of 257 sheep from 10 breeds; North Country Cheviot (NCC), Cheviot (CHE), Dorset (DOR), Suffolk (SUF), Scottish Blackface (SBF), Texel (TEX), Romanov (ROM), Finnish Landrace (FIN), Icelandic (ICE) and Red Masai (MAS) was assessed using 10 microsatellite loci. The average number of alleles per locus was 4.3 in ROM, 5.0 in MAS, and spanned a narrow range (5.4 to 6.0) in the other breeds. Estimates of expected heterozygosity (HE) of the breeds varied within 0.05 point of each other (0.62 in FIN to 0.67 in CHE), except for ROM (0.53) which was lower (P < 0.05) than those of the other breeds, and in MAS (0.57), which was lower (P < 0.05) than those of NCC, CHE and SBF. Estimates of observed heterozygosity (HO) of the breeds were the lowest in MAS, ROM and SUF (0.50 to 0.53) and the highest in NCC and CHE (0.64 and 0.67). The HE was greater (P < 0.01) than HO only in SUF. The results suggest that there have not been drastic losses of genetic variability in the intensely selected breeds. The low genetic variability of ROM was probably due to a small number of animals imported to North America. The British breeds (NCC, CHE, SUF, DOR, SBF) were genetically close to each other, as were the North European breeds (ROM, ICE, FIN). MAS was remotely related to the British breeds, but it was surprisingly close to the North European breeds. TEX was more closely related to the British breeds than to the North European breeds. More than 90% of 1000 simulated individuals from each breed were assigned to the correct breed, indicating that this panel of markers is useful for the identification of breed membership of individual animals, and could be used to protect the integrity of registered breeds. Key words: Sheep, genetic variability, genetic distance, microsatellites

Download Full-text

Analysis of variance of partial diallel tables

Genetics and Molecular Biology ◽

10.1590/s1415-47572000000100040 ◽

2000 ◽

Vol 23 (1) ◽

pp. 229-234 ◽

Cited By ~ 1

Author(s):

José Marcelo Soriano Viana ◽

Cosme Damião Cruz ◽

Antonio Américo Cardoso ◽

Adair José Regazzi

Keyword(s):

Genetic Variability ◽

Common Bean ◽

Statistical Tests ◽

Diallel Analysis ◽

The Other ◽

F1 Hybrids ◽

Dominance Testing ◽

The Common ◽

The Mean ◽

Partial Diallel

The theory of variance analysis of partial diallel tables, following Hayman's proposal of 1954, is presented. As several statistical tests yield similar inferences, the present analysis mainly proposes to assess genetic variability in two groups of parents and to study specific, varietal and mean heteroses. Testing the nullity of specific heteroses equals testing absence of dominance. Testing equality of varietal heteroses of the parents of a group is equivalent to testing the hypothesis that in the other group allelic genes have the same frequency. Rejection of the hypothesis that the mean heterosis is null indicates dominance. The information obtained complements that provided by diallel analysis involving parents and their F1 hybrids or F2 generations. An example with the common bean is included.

Download Full-text

The use of random amplified polymorphic DNA to evaluate the genetic variability of Ponkan mandarin (Citrus reticulata Blanco) accessions

Genetics and Molecular Biology ◽

10.1590/s1415-47572000000100031 ◽

2000 ◽

Vol 23 (1) ◽

pp. 169-172 ◽

Cited By ~ 8

Author(s):

Helvécio Della Coletta Filho ◽

Marcos Antonio Machado ◽

M. Luiza P.N. Targon ◽

Jorgino Pompeu Jr.

Keyword(s):

Genetic Variability ◽

Rapd Analysis ◽

Random Amplified Polymorphic Dna ◽

Point Mutations ◽

The Other ◽

Citrus Reticulata ◽

Random Primers ◽

Citrus Reticulata Blanco ◽

Ponkan Mandarin

RAPD analysis of 19 Ponkan mandarin accessions was performed using 25 random primers. Of 112 amplification products selected, only 32 were polymorphic across five accessions. The absence of genetic variability among the other 14 accessions suggested that they were either clonal propagations with different local names, or that they had undetectable genetic variability, such as point mutations which cannot be detected by RAPD.

Download Full-text

Analysis of intraspecific polymorphism of Nitraria sibirica Pall. using the ISSR technique

BIO Web of Conferences ◽

10.1051/bioconf/20213800054 ◽

2021 ◽

Vol 38 ◽

pp. 00054

Author(s):

Sofia A. Khozyaykina ◽

Evgeny V. Banaev

Keyword(s):

Genetic Variability ◽

Genetic Distance ◽

Dna Polymorphism ◽

Natural Populations ◽

Dna Amplification ◽

Issr Marker ◽

Intraspecific Polymorphism ◽

Nitraria Sibirica ◽

The Republic ◽

Issr Primers

The analysis of DNA polymorphism of Nitraria sibirica Pall. was carried out at 13 natural populations of the Republic of Altai and Altai Territory using the ISSR technique. Seven effective ISSR primers have been identified to analyze DNA polymorphism in N. sibirica. 99 DNA fragments were yielded at DNA amplification with these primers, 66 of them were polymorphic. The genetic distance Nei (D) between the studied populations of N. sibirica averaged 0.32, at mean 0.09 - within populations. An identification ISSR marker has been revealed, which can be used to study the genetic variability of the genus Nitraria L. (Nitrariaceae) species.

Download Full-text

Study of genetic variability of umbilical cord blood using RAPD assay

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v20i4.54144 ◽

2021 ◽

Vol 20 (4) ◽

pp. 848-854

Author(s):

Md Faruque Miah ◽

Md Shad Ebna Rahaman ◽

Sanjana Fatema Chowdhury ◽

Mohammad Golam Rob Mahmud

Keyword(s):

Genetic Variability ◽

Genetic Distance ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Gene Diversity ◽

Human Subjects ◽

Random Amplified Polymorphic Dna ◽

Medical Science ◽

Wiener Index

Background: The genetic variability of Umbilical Cord Blood (UCB) is most important for newborn screening, therapeutic possibility of haematological disorders as well as for the establishment of cord blood banking and stem cell research. Method: Genetic variability of umbilical cord blood (UCB) of 22 human subjects was evaluated first time by applying Random Amplified Polymorphic DNA (RAPD) assay using six decamar primers (B-14, OPB-05, OPB-08, OPB-12, OPB-19 and UBC-122). Result: A total number of bands were recorded 312 from 116 polymorphic loci and single monomorphic locus. All the markers showed highest polymorphism (100%) except the primer OPB 08 (92.31%) among tested individuals. The genetic distance was observed with highest 1.0 and lowest 0.72 respectively whereas mean genetic distance was recorded 0.90. Considering Shannon-Wiener index average diversity was recorded 0.139365. The mean Nei genetic similarity was found 0.17 which was found opposition to genetic distances. A phylogenetic relationship among the individual subjects was also observed between the linkage distances of 11 to 27 with 8 clades, 3 subclusters and a cluster. In addition, average allele frequency p and q was observed 0.08156 and 0.948751 respectively whereas highest intra locus gene diversity and average gene diversity were found 3.323817 and 0.144572 respectively. Conclusion: Considering different parameters, higher genetic variability was found among the experimental subjects, probably due to the mixture DNA of parents and newborn. Bangladesh Journal of Medical Science Vol.20(4) 2021 p.848-854

Download Full-text

Study of genetic differences among Slovak Tsigai populations using microsatellite markers

Czech Journal of Animal Science ◽

10.17221/1670-cjas ◽

2009 ◽

Vol 54 (No. 10) ◽

pp. 468-474 ◽

Cited By ~ 3

Author(s):

S. Kusza ◽

E. Gyarmathy ◽

J. Dubravska ◽

I. Nagy ◽

A. Jávor ◽

...

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Genetic Distance ◽

Microsatellite Markers ◽

Mating System ◽

Population Differentiation ◽

Genetic Relationships ◽

The Other ◽

Potential Risks ◽

Substantial Degree

In this study genetic diversity, population structure and genetic relationships of Tsigai populations in Slovakia were investigated using microsatellite markers. Altogether 195 animals from 12 populations were genotyped for 16 microsatellites. 212 alleles were detected on the loci. The number of identified alleles per locus ranged from 11 to 35. In the majority of the populations heterozygosity deficiency and potential risks of inbreeding could be determined. High values of <I>F</I><sub>ST</sub> (0.133) across all the loci revealed a substantial degree of population differentiation. The estimation of genetic distance value showed that the Slovak Vojin population was the most different from the other populations. The 12 examined populations were able to group into 4 clusters. With this result our aim is to help the Slovak sheep breeders to establish their own mating system, to avoid genetic loss and to prevent diversity of Tsigai breed in Slovakia.

Download Full-text

Application of 7 STR markers for parentage testing and genetic distance study of Equidae / Zastosowanie siedmiu markerów STR do weryfikacji pochodzenia i badania dystansu genetycznego u koniowatych

Annals of Animal Science ◽

10.2478/aoas-2013-0005 ◽

2013 ◽

Vol 13 (2) ◽

pp. 229-239

Author(s):

Ewelina Semik ◽

Tomasz Ząbek

Keyword(s):

Genetic Distance ◽

Tandem Repeats ◽

The Other ◽

Sequence Structure ◽

Fragment Analysis ◽

Str Markers ◽

Parentage Testing ◽

Przewalski Horse ◽

Suitable Marker ◽

Allelic Differentiation

Abstract The genotyping efficiency and polymorphism of 7 microsatellite markers (AHT084, COR006, COR017, COR018, COR040, COR055, COR088) was evaluated in order to apply them to parentage testing among a number of warm-blooded, cold-blooded and primitive horse breeds and to illustrate genetic differences between the breeds investigated. The amplification and sequence structure of these STR markers was also verified in other Equidae like zebra, kulan, donkey and Przewalski horse. Microsatellite allelic differentiation was similar to the allele numbers reported, with an extremely wide allelic range observed at AHT084 locus. However, due to genotyping difficulties AHT084 is not a suitable marker for parentage testing. The use of the other 6 STR markers among most of the horse breeds studied allows excluding wrongly assigned parentage with a probability of 0.99. Fragment analysis and sequencing of STR alleles confirmed the presence of investigated tandem repeats in other Equidae species. Clustering of investigated horse breeds on the tree of Fst distance was consistent with their breeding history, clearly separating breeds into 3 horse types mentioned above.

Download Full-text

Pollination biology and genetic variability of a giant perfumed flower (Aristolochia gigantea Mart. and Zucc., Aristolochiaceae) visited mainly by small Diptera

Botany ◽

10.1139/b2012-047 ◽

2012 ◽

Vol 90 (9) ◽

pp. 815-829 ◽

Cited By ~ 10

Author(s):

Juliana Hipólito ◽

Blandina Felipe Viana ◽

Alessandra Selbach-Schnadelbach ◽

Leonardo Galetto ◽

Peter G. Kevan

Keyword(s):

Genetic Variability ◽

The Other ◽

Floral Traits ◽

Pollination Syndrome ◽

Chapada Diamantina ◽

Species Characteristics ◽

Structural Plan ◽

Low Levels ◽

Compatible Pollen ◽

And Behavior

The genus Aristolochia presents conserved features in its basic structural plan of trap flowers and in its pollination syndrome. Visitors, usually flies, are attracted to the rotting-meat scented flowers and remain trapped until the second day, when the protogynous flowers release them. Aristolochia gigantea Mart. and Zucc showed many of these floral traits but display a citronella-like odour, giving the opportunity to study the reproductive ecology in this atypical species. Characteristics and behavior of pollinators, coupled with the distances between individual plants and populations, are important factors in patterns of genetic diversity. Thus, our objectives were to study the flower visitors, reproductive success, and gene flow in A. gigantea in the Chapada Diamantina, Bahia, Brazil. Results suggest that pollination by flies remains as a conserved interaction within the genus Aristolochia. The main pollinators appeared to be Megaselia spp. (Phoridae), evidenced primarily by the frequency of their occurrence within flowers and the transference of compatible pollen. On the other hand, the moderate to low levels of intrapopulation genetic variability found in A. gigantea, combined with low effective pollination and its reproductive dependence on biotic vectors, indicate that this species may be suffering gene erosion in the remaining populations located at Chapada Diamantina.

Download Full-text