scholarly journals Studies on DNA Polymorphism of Silver Maple (Acer saccharinum L.)

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 783A-783
Author(s):  
A. Virginia Freire ◽  
John E. Preece ◽  
David A. Lightfoot
Keyword(s):  
Genetic Relationship ◽  
Dna Polymorphism ◽  
Rapd Analysis ◽  
Pcr Amplification ◽  
Cesium Chloride ◽  
Biomass Feedstock ◽  
Red Maple ◽  
Silver Maple ◽  
Natural Range

Silver maple has great potential as a biomass feedstock. We compared three clones from each of seven provenances located on east to west and north to south transects across the natural range of silver maple and one red maple. DNA extracted by a modification of the CTAB technique (Murray and Thompson, 1980) was not suitable for RAPD analysis. Using this technique, polymorphism was either not reproducible or there was poor amplification for some clones. A new DNA extraction technique using PVPP, chloroform, and cesium chloride was tested (a modification of Yoon et al., 1991). this method yielded DNA that was more suitable for PCR amplification. Both RAPD and DAF (Caetano-Anolles and Gresshoff, 1994) methods were used for amplification. Polymorphism was detected among and within provenances. DAF was more efficient than RAPDs for determination of the genetic relationship among silver maple clones.

scholarly journals 665 PB 107 DNA POLYMORPHISM IN SILVER MAPLE (ACER SACCHARINUM L.)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 528b-528
Author(s):  
A. Virginia Freire ◽  
John E. Preece ◽  
David A. Lightfoot
Keyword(s):  
Polymerase Chain Reaction ◽  
High Frequency ◽  
Interspecific Hybrids ◽  
Dna Polymorphism ◽  
Biomass Feedstock ◽  
Natural Area ◽  
Chain Reaction ◽  
Silver Maple ◽  
Polymerase Chain

Silver maple has great potential as a biomass feedstock. We selected 21 elite silver maple clones representing 7 provenances located on east to west and north to south transects across the natural area of distribution. In addition five different red maples including one commercial cultivar as well as four interspecific hybrids between red and silver maple were compared to the silver maples. DNA was extracted using a modification of the CTAB technique (Murray and Thompson, 1980). Polymerase chain reaction was used with random primers from the OPF series (1-20) and primers used by Krahl et al. (1993). Polymorphism was detected at high frequency. Greater polymorphism was observed between species than within species. However, we have observed DNA concentration dependent polymorphism. RAPD technology has potential for determination of genetic relationship among silver maple clones.

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

2011 ◽  
Vol 14 (2) ◽  
pp. 285-286 ◽  
Author(s):  
J. Karakulska ◽  
A. Pobucewicz ◽  
P. Nawrotek ◽  
M. Muszyńska ◽  
A. Furowicz ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Species Identification ◽  
Genetic Relationship ◽  
Molecular Typing ◽  
Rapd Analysis ◽  
Milk Samples ◽  
Rapd Pcr ◽  
Pcr Rflp ◽  
Mastitic Milk ◽  
Coa Gene

Molecular typing ofStaphylococcus aureusbased on PCR-RFLP ofcoagene and RAPD analysisThe aim of this study was molecular identification ofS. aureusstrains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains thegapgene (930 bp) was amplified, which enabled their affiliation to theStaphylococcusgenus to be established. PCR-RFLP withAluI endonuclease of thegapgene as well asnuc(450 bp) andcoa(1130 bp) gene amplification allowed preciseS. aureusspecies identification. One hundred percent of the genetic relationship between strains was establishedviaRAPD-PCR and coa-typing.

scholarly journals Direct Profiling of Environmental Microbial Populations by Thermal Dissociation Analysis of Native rRNAs Hybridized to Oligonucleotide Microarrays

2003 ◽  
Vol 69 (4) ◽  
pp. 2377-2382 ◽  
Author(s):  
Said El Fantroussi ◽  
Hidetoshi Urakawa ◽  
Anne E. Bernhard ◽  
John J. Kelly ◽  
Peter A. Noble ◽  
...  
Keyword(s):  
Thermal Dissociation ◽  
Pcr Amplification ◽  
Estuarine Sediments ◽  
Microbial Populations ◽  
Oligonucleotide Microarrays ◽  
Dissociation Curve ◽  
Melting Profile ◽  
Probe Target ◽  
Study Sites

ABSTRACT Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences.

Genetic Relationship Among Tephrosia Species as Revealed by RAPD Analysis

2007 ◽  
Vol 1 (1) ◽  
pp. 1-10 ◽  
Author(s):  
P. Lakshmi ◽  
P. Akbar Ali Khan ◽  
P. Narasimha Reddy ◽  
K. Lakshminar ◽  
S. Ganapaty
Keyword(s):  
Genetic Relationship ◽  
Rapd Analysis

scholarly journals Molecular Characterization of Yarrowia lipolytica and Candida zeylanoides Isolated from Poultry

2000 ◽  
Vol 66 (10) ◽  
pp. 4340-4344 ◽  
Author(s):  
T. Deak ◽  
J. Chen ◽  
L. R. Beuchat
Keyword(s):  
Yarrowia Lipolytica ◽  
Rapd Analysis ◽  
Restriction Analysis ◽  
Pcr Amplification ◽  
Yeast Cells ◽  
Chromosomal Dna ◽  
Intergenic Sequences ◽  
Species Specific ◽  
Candida Zeylanoides ◽  
Its Pcr

ABSTRACT Yeast isolates from raw and processed poultry products were characterized using PCR amplification of the internally transcribed spacer (ITS) 5.8S ribosomal DNA region (ITS-PCR), restriction analysis of amplified products, randomly amplified polymorphic DNA (RAPD) analysis, and pulsed-field gel electrophoresis (PFGE). ITS-PCR resulted in single fragments of 350 and 650 bp, respectively, from eight strains of Yarrowia lipolytica and seven strains of Candida zeylanoides. Digestion of amplicons with HinfI andHaeIII produced two fragments of 200 and 150 bp fromY. lipolytica and three fragments of 350, 150, and 100 bp from C. zeylanoides, respectively. Although these fragments showed species-specific patterns and confirmed species identification, characterization did not enable intraspecies typing. Contour-clamped heterogeneous electric field PFGE separated chromosomal DNA of Y. lipolytica into three to five bands, most larger than 2 Mbp, whereas six to eight bands in the range of 750 to 2,200 bp were obtained from C. zeylanoides. Karyotypes of both yeasts showed different polymorphic patterns among strains. RAPD analysis, using enterobacterial repetitive intergenic sequences as primers, discriminated between strains within the same species. Cluster analysis of patterns formed groups that correlated with the source of isolation. For ITS-PCR, extraction of DNA by boiling yeast cells was successfully used.

Genotyping of Acanthamoeba Species Isolated from Keratitis Patients by PCR Sequencing Methods in Tehran, Iran

2019 ◽  
Author(s):  
Tooran Nayeri Chegeni ◽  
Fatemeh Ghaffarifar ◽  
Majid Pirestani ◽  
Fariba Khoshzaban ◽  
Abdolhosein Dalimi Asl ◽  
...  
Keyword(s):  
Contact Lenses ◽  
Pcr Amplification ◽  
Acanthamoeba Keratitis ◽  
Local Alignment ◽  
Sequencing Analysis ◽  
Search Tool ◽  
Skin Nodules ◽  
Acanthamoeba Species ◽  
Eye Clinic

  Background and Aims: Amoebae of the genus Acanthamoeba are unicellular amphizoic opportunistic pathogens that may cause fatal granulomatous encephalitis, eye keratitis, amebic pneumonitis and skin nodules as well as abscesses in humans and animals. Acanthamoeba keratitis is caused by trauma to the eye, contaminated cleaning solutions and the use of contact lenses. The aim of the present study was to identify the genotypes of Acanthamoeba in all patients with a clinical diagnosis of Acanthamoeba keratitis referring to eye clinic in Tehran using polymerase chain reaction (PCR).  Materials and Methods: In this study, samples were collected from 35 patients who had referred to the eye clinic and were cultured on 1.5% non-nutrient agar. DNA was extracted, and then PCR amplification was performed using genus specific primers. Sequencing analysis and basic local alignment search tool search were conducted to determine the genotypes. Phylogenetic tree was generated using maximum likely algorithm in phylogenetic program MEGA version 6.  Results: Eight cases were positive for Acanthamoeba using genus specific primer pairs. All specimens were reported as genotype T4. Conclusions: Determination of genotypes showed all isolates belonging to genotype T4; this abundance may be due to its higher prevalence in the environment or its greater virulence. However, further analysis of clinical and environmental samples is necessary to clarify this property.   

Classifying forestland from model-generated tree species habitat suitability in the Western Ecoregion of Nova Scotia, Canada

2013 ◽  
Vol 43 (6) ◽  
pp. 517-527 ◽  
Author(s):  
Mark Baah-Acheamfour ◽  
Charles P.-A. Bourque ◽  
Fan-Rui Meng ◽  
D. Edwin Swift
Keyword(s):  
Nova Scotia ◽  
Tree Species ◽  
Habitat Suitability ◽  
Sugar Maple ◽  
Forest Succession ◽  
Red Maple ◽  
White Birch ◽  
Permanent Sample Plots ◽  
Natural Range ◽  
Hybrid Classification

Forestland classification is central to the sustainable management of forests. In this paper, we explore the possibility of classifying forestland from species–habitat–suitability indices and a hybrid classification of modeled data. Raster-based calculations of species–habitat–suitability were derived as a function of landscape-level descriptions of incident photosynthetically active radiation (PAR), soil water content (SWC), and growing degree-days (GDD) for southwestern Nova Scotia, Canada. PAR and SWC were both generated with the LanDSET model and GDD from thermal data captured with the space-borne MODIS sensor. We compared the distribution of predicted forestland types with the natural range of target species as found in the provincial permanent sample plots (PSPs). Reasonable agreement (≥50% accuracy) existed between some forestland types (e.g., red maple – white birch – red oak and balsam fir – red maple) and PSP-based assessments of species presence–absence. Agreement was noticeably lower for other forestland types, such as sugar maple – beech – yellow birch (<50% accuracy). This discrepancy is attributed to forest-forming factors not directly addressed by the model, e.g., forest succession, stand interventions, and disturbance. Their addition in the model could change the dynamics of tree-species preference in southwest Nova Scotia and is worth examining. True model inaccuracies accounted for about 0.3%–15.0% of the total reported error.

scholarly journals Determination of the Synergism/Antagonism Parameters during Co-gasification of Potassium-Rich Biomass with Non-biomass Feedstock

2017 ◽  
Vol 31 (2) ◽  
pp. 1842-1849 ◽  
Author(s):  
Roxin Fernandes ◽  
Josephine M. Hill ◽  
Jan Kopyscinski
Keyword(s):  
Biomass Feedstock
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