Nitrite Accumulation and Nitrogen Immobilization in Potting Media Containing Compost
Growth inhibition has been observed with plants grown in potting media containing compost. The objective of this study was to determine if \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{NO}_{2}^{-}\) \end{document} toxicity or N immobilization might be involved. Two potting media were blended from aged pinebark, vermiculite, sphagnum peat, and compost, along with a control medium without compost, and cropped with Dendranthema ×grandiflorum `Bravo'. Pots were fertilized weekly with a 15N–1.1P–12.5K soluble fertilizer at 24 mM N. Plants were harvested and media samples were collected at intervals. Saturated media extracts (SME) were prepared immediately. Separate samples were incubated at 25C for 4 days following addition of 2.5 mmol urea-N/cm3. In SME of one compost-containing medium, \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{NO}_{2}^{-}\) \end{document} was present at 0.25 ± 0.03 mM 2 weeks after transplant, and at progressively lower concentrations thereafter. In incubated samples of the same medium, accumulation of \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{NO}_{2}^{-}\) \end{document} was observed after 3 weeks of cropping, with peak accumulation of \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(0.7{\pm}0.32{\ }{\mu}{\ }\mathrm{mol}\mathrm{NO}_{2}^{-}{/}\mathrm{cm}^{3}\) \end{document} after 9 weeks of cropping. Nitrite was scarcely detectable in other media. Some indication of N immobilization was obtained, as NH+4levels decreased during incubation without any increase in \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{NO}_{3}^{-}\) \end{document}, and \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{NO}_{3}^{-}\) \end{document} present at the beginning of incubation disappeared during incubation. In this study, significant effects of media on plant growth were not related to differences in \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{NO}_{2}^{-}\) \end{document} accumulation or N immobilization. However, subsequent studies appear to have established such a relationship.