scholarly journals Inheritance of Resistance to Halo Blight Flower and Stem Color and Association in Common Beans

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 621d-621
Author(s):  
H.M. Ariyarathne ◽  
D.P. Coyne
Keyword(s):  
Pseudomonas Syringae ◽  
Recombinant Inbred Lines ◽  
Major Gene ◽  
Segregation Ratio ◽  
Inbred Lines ◽  
Gene Control ◽  
Common Beans ◽  
Bacterial Diseases ◽  
Halo Blight ◽  
Ri Lines

Halo blight is one of the most important bacterial diseases of common beans (Phaseolus vulgaris L.). It is serious under moderate temperature and high humidity conditions. The disease is caused by a seed-borne bacterium, Pseudomonas syringae pv. phaseolicola (Burkh.) Dowson (Psp). The inheritance of leaf reactions to Psp, flower, and stem color was studied using greenhouse-grown 109 F9 recombinant inbred lines (RI) from the P. vulgaris cross BelNeb 1 [resistant (R)] (USDA/NE) × A 55 [susceptible (S)] (CIAT). Two Psp strains, HB16 (NE) and 83-Sc2A (NE), were inoculated using the water-soaking method. A segregation ratio of 1 R:1 S RI lines were observed for disease reactions in leaves for both strains indicating major gene control. The presence of recombinants for SR, RS to the strains indicated that different genes were involved. Stem (SC) and flower (FC) color traits were each determined by two major genes. Linkages were found for reactions to the two Psp strains and also between FC and SC. No linkages were observed from FC and also SC with reactions to Psp strains.

scholarly journals Construction of a Genetic Linkage Map and Locations of Halo Blight and Brown Spot Resistance Loci in Common Bean (Phaseolus vulgaris L.) using RAPD Markers

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 451D-451
Author(s):  
H.M. Ariyarathne ◽  
Dermot P. Coyne ◽  
Geunhwa Jung
Keyword(s):  
Linkage Map ◽  
Pseudomonas Syringae ◽  
Phenotypic Variation ◽  
Rapd Markers ◽  
Major Gene ◽  
Interval Mapping ◽  
Brown Spot ◽  
Halo Blight ◽  
Genomic Regions ◽  
Ri Lines

Halo blight (HB), brown spot (BS), and rust incited by the bacterial pathogens Pseudomonas syringae pv. phaseolicola (Psp), Pseudomonas syringae pv. syringae (Pss) and the fungal pathogen Uromyces appendiculatus, respectively, are important diseases of common beans. The objectives were to construct a RAPD linkage map, and to locate HB and BS resistance genes and genes for some other traits. One-hundred-seventy RAPD markers were mapped in 78 RI lines of the cross BelNeb 1 and A 55. Eleven main and nine minor linkage groups were identified. MAPMAKER/QTL, interval mapping, was used to identify genomic regions involved in the genetic control of the traits. One region was found to control HB leaf reactions to strain HB16 while three regions controlled reactions to strain HB 83. These regions accounted for 22% and 18%, 17%, and 17% of phenotypic variation of resistance, respectively. Four putative QTLs were identified for resistance to BS, and accounted for 37%, 26%, 23%, and 19% of the phenotypic variation. Rust resistance was determined by a single major gene to both rust strains US85NP 5-1 and D82vc74fh. However, linked markers were not identified. The V gene controlling flower and stem color was tightly linked with the Operon marker O10.620.

Pathogenic Variation in Halo Blight on Common Beans

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 500f-501
Author(s):  
H.M. Ariyarathne ◽  
D.P. Coyne ◽  
A.K. Vidaver
Keyword(s):  
Pseudomonas Syringae ◽  
Block Design ◽  
Incomplete Block ◽  
Common Beans ◽  
Bacterial Diseases ◽  
Halo Blight ◽  
Pathogenic Variation ◽  
Split Plot ◽  
Differential Cultivars ◽  
New Races

Halo blight, caused by a bacterium, Pseudomonas syringae pv. phaseolicola (Psp), is one of the most important bacterial diseases of common beans (Phaseolus vulgaris L.). The objective of this study was to evaluate the pathogenic variation of Psp strains with emphasis on those collected in Nebraska. Twenty-nine Psp strains were inoculated on 20-day-old leaves of eight differential cultivars/lines and a resistant check line great northern (GN) Nebraska #1 sel. 27. Two experiments were conducted in growth chambers under 20 °C and 12-h photoperiod. A split-plot design was used with nine cultivars/lines as whole plots in a randomized complete block design (RCBD) and strains as sub-plots in an incomplete block arrangement. Data were analyzed as a split-plot with a RCB arrangement because incomplete block was not significant. Strains were classified into five races with six, 16, and two strains classified as races 1, 6, and 7, respectively. The remaining strains were designated tentatively as new races 10 and 11. GN Nebraska #1 sel. 27 was resistant to all Psp strains tested. This information is useful to breeders interested in developing germplasm and cultivars resistant to Psp.

scholarly journals A major autosomal gene effect on activity of glucose 6-phosphate dehydrogenase segregating between recombinant inbred lines of mice

1980 ◽  
Vol 36 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Robert P. Erickson ◽  
Kenneth Harper
Keyword(s):  
Recombinant Inbred ◽  
Coat Color ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Autosomal Gene ◽  
Parental Line ◽  
Males And Females ◽  
Parental Lines ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Ri Lines

SUMMARYG6PD measurements (expressed per g haemoglobin) in both males and females of the parental lines C57BL/6J and C57L/J and four recombinant inbred (RI) lines between them showed segregation of G6PD levels as parental classes (one line showed the higher C57BL/6J activity, three lines were indistinguishable from C57L/J). Haemoglobin also varied significantly among the parental and RI lines but this variation did not explain the variation in G6PD. NADP levels (expressed per g wet wt of erythrocytes) correlated with G6PD levels but one RI line was intermediate in males while two RI lines were lower than either parental line in females. Thus, the major autosomal locus affecting G6PD levels which segregates between these two inbred lines probably does not act directly on NADP levels. The variation in the G6PD levels in the four RI lines co-segregated with the brown coat color locus but the association is not significant because of the small number of RI lines available.

scholarly journals Identification of Gray Leaf Spot Disease Candidate Gene in Narrow-Leafed Lupin (Lupinus angustifolius L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Gaofeng Zhou ◽  
Huaan Yang ◽  
Daniel Renshaw ◽  
Meilin Zou ◽  
Geoff Thomas ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Leaf Spot ◽  
Recombinant Inbred Lines ◽  
Major Gene ◽  
Segregation Ratio ◽  
Gray Leaf Spot ◽  
Wild Accession ◽  
Leaf Spot Disease ◽  
Breeding Line ◽  
Phylogenetic Tree Analysis

Selection for resistance against gray leaf spot (GLS) is a major objective in the lupin breeding programs. A segregation ratio of 1:1 (resistant:susceptible) in F8 recombinant inbred lines (RIL8) derived from a cross between a breeding line 83A:476 (resistant to GLS) and a wild accession P27255 (susceptible to GLS) indicated that GLS was controlled by a single major gene. To develop molecular markers linked to GLS, in the beginning, only 11 resistant lines and six susceptible lines from the 83A:476 and P27255 population were genotyped with MFLP markers, and three MFLP markers were identified to be co-segregated with GLS. This method was very efficient, but the markers were located outside of the gene, and could not be used in other germplasms. Then QTL analysis and fine mapping were conducted to identify the gene. Finally, the gene was narrowed down to a 241-kb region containing two disease resistance genes. To further identify the candidate gene, DNA variants between accessions Tanjil (resistant to GLS) and Unicrop (susceptible to GLS) were analyzed. The results indicated that only one SNP was detected in the 241 kb region. This SNP was located in the TMV resistance protein N-like gene region and also identified between 83A:476 and P27255. Genotyping the Tanjil/Unicrop RIL8 population showed that this SNP co-segregated with GLS resistance. The phylogenetic tree analysis of this gene among 18 lupin accessions indicates that Australian resistant breeding line/varieties were clustered into one group and carry two resistant alleles, while susceptible accessions were clustered into different groups.

scholarly journals Inheritance; Low Correlations of Leaf, Pod, and Seed Reactions to Common Blight Disease in Common Beans; and Implications for Selection

1994 ◽  
Vol 119 (1) ◽  
pp. 116-121 ◽  
Author(s):  
Eladio Arnaud-Santana ◽  
D.P. Coyne ◽  
K.M. Eskridge ◽  
A.K. Vidaver
Keyword(s):  
Recombinant Inbred Lines ◽  
Genetic Correlations ◽  
Inbred Lines ◽  
Breeding Strategy ◽  
Common Beans ◽  
Leaf Pubescence ◽  
Dry Bean ◽  
The Cross ◽  
Blight Disease ◽  
Pearson Correlations

The heritability of leaf, pod, and seed reactions; stem color and abaxial leaf pubescence; and the association of these traits were studied in advanced dry bean recombinant inbred lines derived from the Phaseolus vulgaris crosses `PC-SO' × XAN-159, `PC-50' × BAC-6, and `Venezuela 44' × BAC-6. The reaction to Xcp was quantitatively inherited in all three plant organs. Qualitative inheritance was found for stem color and leaf pubescence. Low to intermediate heritability values were obtained for the leaf and seed reactions to Xcp. Heritability estimates were consistently low for the pod reaction to Xcp. Low nonsignificant Pearson correlations were detected between leaf and pod reactions, leaf and seed reactions, and pod and seed reactions, except for the latter two correlations, which were low and significant in lines from the cross `PC-50' × XAN-154. Genetic correlations between leaf and pod reactions and leaf and seed reactions were low and significant in lines from all crosses, except for Venezuela 44 × BAC-6 in the latter case. Genetic correlations between pod and seed reactions were low and nonsignificant, except in the cross `PC-50' × XAN-159, for which a low significant correlation was observed. No significant association was found between Xcp leaf reaction and stem color or leaf pubescence. A breeding strategy for improving resistance to Xcp in P. vulgaris is discussed.

scholarly journals Diagnosis of leaf bacterial diseases of coffee reveals the prevalence of halo blight

2021 ◽  
Vol 45 ◽  
Author(s):  
Melina Korres Raimundi ◽  
Ricardo Magela de Souza ◽  
Antônia dos Reis Figueira ◽  
Gustavo Matheus Silva ◽  
Ana Carolina de Paula Santos ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Leaf Spot ◽  
Biochemical Properties ◽  
Minas Gerais ◽  
Bacterial Leaf Spot ◽  
Bacterial Diseases ◽  
Halo Blight ◽  
Rpod Gene ◽  
Minas Gerais State ◽  
Species Specific

ABSTRACT The diagnosis of foliar bacterial diseases in coffee (Coffea arabica), such as halo blight (Pseudomonas syringae pv. garcae), bacterial leaf spot (P. syringae pv. tabaci), bacterial blight (P. cichorii), and dark leaf spot (Robbsia andropogonis), is considered a challenge for plant pathologists. The misidentification has been occurring when the diagnosis is solely based on symptoms and biochemical properties. Thus, the objective of this study was to identify and differentiate species and pathovars of Pseudomonas pathogenic to coffee plants, enabling a survey of the occurrence of these bacteria in the main producing regions of Minas Gerais state, Brazil. Firstly, the pathogenicity of the isolates was confirmed by inoculation in C. arabica cv. Catuaí Vermelho IAC 99. Then, biochemical analyses, combined with, repetitive element-polymerase chain reaction (rep-PCR) and phylogeny based on rpoD gene sequences were used to characterize 84 Pseudomonas isolates from coffee crops and nurseries. Based on rpoD-phylogeny, 73 isolates were identified as P. syringae pv. garcae, five as P. syringae pv. tabaci and six as P. cichorii. The rep-PCR results suggest a high genetic variability in populations of Pseudomonas syringae pv. garcae and P. cichorii. This is the first report of the occurrence of bacterial leaf spot (P. syringae pv. tabaci) in the coffee-producing filed in Minas Gerais State. The findings confirmed the prevalence of P. syringae pv. garcae in coffee production fields in the State and the generated knowledge will contribute for the development of species-specific primers for the identification and detection of this pathogen.

scholarly journals Heritability of Resistance and Associations of Leaf, Pod, and Seed Reactions to Xanthomonas campestris pv. Phaseoli (DYE) in Common Beans

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 840A-840
Author(s):  
H.M. Ariyarathne ◽  
D.P. Coyne ◽  
A.K. Vidaver ◽  
K. Eskridge
Keyword(s):  
Phaseolus Vulgaris ◽  
Xanthomonas Campestris ◽  
Recombinant Inbred Lines ◽  
Genetic Correlations ◽  
Flower Color ◽  
Inbred Lines ◽  
Seed Infection ◽  
Quantitative Inheritance ◽  
Common Beans ◽  
Plant Organs

The inheritance and heritability (H) of leaf and pods reactions and seed infection of common beans (Phaseolus vulgaris L.) to Xanthomonas campestris pv. phaseoli (Smith) Dye (Xcp) were studied in three crosses along with flower and stem color, and the association of reactions to Xcp in the plant organs. Recombinant inbred lines from the crosses `PC 50' × XAN 159, BAC 6 × HT 7719, and BelNeb 1 × A 55 were used. Quantitative inheritance patterns were observed for disease reactions in leaves, pods, and seeds. Stem and flower color were inherited qualitatively. Low to intermediate and intermediate H estimates were found for pod reactions when inoculated on the same time, allowing the infection to occur in a uniform environment. Intermediate to high H estimates were found for leaf and seed reactions to Xcp, respectively. Significant positive intermediate to moderately high correlations were found between the reactions to Xcp of the first trifoliolate with later-developed leaves and pods in all three populations. The moderately high genetic correlations between leaves and pods suggested that some common genes may control the reactions to Xcp in these plant organs. No association was detected between flower or stem color and reactions to Xcp.

Diverse sources of resistance to Spodoptera litura (F.) in groundnut

2019 ◽  
Vol 79 (01S) ◽  
Author(s):  
M. A. Saleem ◽  
G. K. Naidu ◽  
H. L. Nadaf ◽  
P. S. Tippannavar
Keyword(s):  
Recombinant Inbred ◽  
Spodoptera Litura ◽  
Hot Spot ◽  
Recombinant Inbred Lines ◽  
Insect Pest ◽  
Inbred Lines ◽  
Leaf Damage ◽  
Botanical Variety ◽  
Resistant Genotypes ◽  
Botanical Varieties

Spodoptera litura an important insect pest of groundnut causes yield loss up to 71% in India. Though many effective chemicals are available to control Spodoptera, host plant resistance is the most desirable, economic and eco-friendly strategy. In the present study, groundnut mini core (184), recombinant inbred lines (318) and elite genotypes (44) were studied for their reaction to Spodoptera litura under hot spot location at Dharwad. Heritable component of variation existed for resistance to Spodoptera in groundnut mini core, recombinant inbred lines and elite genotypes indicating scope for selection of Spodoptera resistant genotypes. Only 29 (15%) genotypes belonging to hypogaea, fastigiata and hirsuta botanical varieties under mini core set, 15 transgressive segregants belonging to fastigiata botanical variety among 318 recombinant inbred lines and three genotypes belonging to hypogaea and fastigiata botanical varieties under elite genotypes showed resistance to Spodoptera litura with less than 10% leaf damage. Negative correlation existed between resistance to Spodoptera and days to 50 per cent flowering indicating late maturing nature of resistant genotypes. Eight resistant genotypes (ICG 862, ICG 928, ICG 76, ICG 2777, ICG 5016, ICG 12276, ICG 4412 and ICG 9905) under hypogaea botanical variety also had significantly higher pod yield. These diverse genotypes could serve as potential donors for incorporation of Spodoptera resistance in groundnut.

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