scholarly journals Anatomy of Adventitious Root Formation in Stem Cuttings of Mussaenda erythrophylla L. `Rosea'

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 634b-634
Author(s):  
Rolston St. Hilaire ◽  
Carlos A. Fierro Berwart
Keyword(s):  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Stem Cuttings ◽  
Root Primordia ◽  
Fast Green ◽  
Phloem Parenchyma ◽  
Ornamental Shrubs ◽  
Vascular Connections

Mussaendas (Mussaenda spp.) are ornamental shrubs, and some cultivars are difficult to root. This study was conducted to explore how adventitious roots initiate and develop in the cultivar Rosea, and to determine if anatomical events are associated with difficulty in rooting stem cuttings. Stem cuttings were treated with 5, 10, 15 mm 1H-indole-3-butyric acid (IBA), or distilled water, and sampled every 2 days over 26 days to observe adventitious root formation and development. For analysis by light microscopy, the basal 1 cm of cuttings was embedded in wax and stained with safranin-fast green. Adventitious roots initiated from phloem parenchyma cells and from basal callus in nontreated cuttings. Cuttings treated with 15 mm IBA had a mean of 18 root primordia per basal 1 cm of cutting after 10 days. Root primordia were not observed in non-treated cuttings at 10 days. Root primordia that developed in non-treated cuttings lacked clear vascular connections. These results suggest that non-treated cuttings are difficult to root because few primordia are produced.

scholarly journals Adventitious Root Formation and Development in Cuttings of Mussaenda erythrophylla L. Schum. & Thonn.

HortScience ◽  
1996 ◽  
Vol 31 (6) ◽  
pp. 1023-1025 ◽  
Author(s):  
Rolston St. Hilaire ◽  
Carlos A. Fierro Berwart ◽  
Carlos A. Pérez-Muñoz
Keyword(s):  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Distilled Water ◽  
Stem Cuttings ◽  
Root Primordia ◽  
Phloem Parenchyma ◽  
Parenchyma Cells ◽  
Ornamental Shrubs

Mussaendas (Mussaenda spp.) are ornamental shrubs, and some cultivars are difficult to root. This study was conducted to explore how adventitious roots initiate and develop in the cultivar Rosea and to determine if anatomical events are associated with difficulty in rooting stem cuttings. Stem cuttings were treated with 5, 10, 15 mmol IBA, or distilled water, and sampled every 2 days over 26 days to observe adventitious root formation and development. Adventitious roots initiated from phloem parenchyma cells. Cuttings treated with 15 mmol IBA had a mean of 18 root primordia per basal 1 cm of cutting after 10 days. Primordia were absent in nontreated cuttings at 10 days. These results suggest that nontreated cuttings are difficult to root because few primordia are produced. Chemical name used: 1H-indole-3-butyric acid (IBA).

scholarly journals Highly Successful Adventitious Root Formation of Zamia L. Stem Cuttings Exhibits Minimal Response to Indole-3-Butyric Acid

HortScience ◽  
2020 ◽  
Vol 55 (9) ◽  
pp. 1463-1467 ◽  
Author(s):  
Benjamin E. Deloso ◽  
Anders J. Lindström ◽  
Frank A. Camacho ◽  
Thomas E. Marler
Keyword(s):  
Butyric Acid ◽  
Root Development ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
The Other ◽  
Control Group ◽  
Stem Cuttings ◽  
Adventitious Root Development

The influences of indole-3-butyric acid (IBA) concentrations of 0–30 mg·g−1 on the success and speed of adventitious root development of Zamia furfuracea L.f. and Zamia integrifolia L.f. stem cuttings were determined. Root formation success for both species was greater than 95%. The IBA concentrations did not influence the speed of root development for Z. furfuracea, but the Z. integrifolia cuttings that received IBA concentration of 3 mg·g−1 generated adventitious roots more slowly than the cuttings in the control group. The ending dry weights of the stems, leaves, and roots were not influenced by IBA concentration for either species. Our results indicated that adventitious root formation on stem cuttings of these two Zamia species is successful without horticultural application of IBA. Additional IBA studies are needed on the other 300+ cycad species, especially those that are in a threatened category.

INITIATION AND DEVELOPMENT OF ADVENTITIOUS ROOTS IN STEM CUTTINGS OF HEDERA HELIX: ANATOMICAL STUDIES OF THE MATURE GROWTH PHASE

1967 ◽  
Vol 45 (10) ◽  
pp. 1883-1886 ◽  
Author(s):  
Ronald M. Girouard
Keyword(s):  
Growth Phase ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Stem Cuttings ◽  
Hedera Helix ◽  
Anatomical Studies ◽  
Ray Parenchyma ◽  
English Ivy

Adventitious root formation was studied microscopically in stem cuttings of a difficult-to-root plant, the mature growth phase of Hedera helix, English ivy. Roots were found initiating at the end of 2–4 weeks in phloem ray parenchyma of internodes with and without woundwood and in callus near the basal end of cuttings; roots emerged at the end of 3–5 weeks. The fibro-bundle caps were thicker than those found in juvenile stems, but they did not hamper the development of adventitious roots.

scholarly journals Roles of Auxin and Ethylene in Adventitious Root Formation by a Flood-resistant Tomato Genotype

HortScience ◽  
1991 ◽  
Vol 26 (1) ◽  
pp. 57-58 ◽  
Author(s):  
Steven T. McNamara ◽  
Cary A. Mitchell
Keyword(s):  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Formation ◽  
Subsequent Development ◽  
Fold Increase ◽  
Adventitious Root Formation ◽  
Silver Thiosulphate ◽  
Root Primordia ◽  
Tomato Genotype ◽  
Auxin Accumulation

The relative contributions of auxin and ethylene (C2H4) in stimulating the initiation of adventitious root primordia (ARP) and their subsequent development into adventitious roots (ARs) by flooded tomato (Lycopersicon esculentum Mill. PI 406966) seedlings were evaluated using TIBA and STS. Flooded plants treated with STS (F + STS) produced ≈ 40% as many emerged ARs as plants that were flooded only (F). Only 7% of the ARP initiated by F + STS plants developed enough to emerge through the epidermis by 120 hours of treatment compared with 95% emerged for F plants. A band of TIBA applied below the lowest leaves of flooded plants (F + TIBA) virtually eliminated AR formation. Plants with two or four leaves below the TIBA band produced 16- and 35-fold more ARs, respectively, than those with no leaves below the TIBA band. Relative to nonflooded (NF) plants, F + STS plants exhibited a nearly 40-fold increase in C2H4 evolution, while F and F + TIBA plants exhibited about a 5-fold increase in C2H4 production. These results suggest that auxin accumulation at or above the floodline is essential for ARP initiation and that auxin action is not mediated through C2H4. Ethylene may be required for elongation of flood-induced ARP leading to their emergence as ARs. Chemical names used: 2,3.5 -triiodobenzoic acid (TIBA): silver thiosulphate (STS).

scholarly journals Gene expression profiling during adventitious root formation in carnation stem cuttings

BMC Genomics ◽  
2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Carlos Villacorta-Martín ◽  
Ana Belén Sánchez-García ◽  
Joan Villanova ◽  
Antonio Cano ◽  
Miranda van de Rhee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Adventitious Root ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Stem Cuttings

scholarly journals Prophylactic Treatments of Cycas Stem Wounds Influence Vegetative Propagation

2020 ◽  
Vol 13 ◽  
pp. 194008292092059 ◽  
Author(s):  
Thomas E. Marler ◽  
Benjamin E. Deloso ◽  
Gil N. Cruz
Keyword(s):  
Adventitious Root ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Petroleum Jelly ◽  
Stem Cuttings ◽  
Open Wound ◽  
Asexual Propagation ◽  
Pruning Wound ◽  
Modeling Clay ◽  
Parenchyma Tissue

Cycad stem cuttings will develop adventitious roots if the open wound is first treated with a sealant to protect the exposed parenchyma tissue. The commercial pruning wound sealant that is often employed for this purpose is not available in many locations. We used commonly available products as a prophylactic sealant on Cycas edentata , Cycas micronesica , and Cycas nitida cuttings to determine efficacy for sealing the wound and enabling adventitious root formation. Success was quantified after 7 months in a sand propagation substrate. Mortality was 100% for control cuttings with no wound sealant and about 60% for cuttings with candle wax as the sealant. Cuttings that received petroleum jelly, lanolin paste, modeling clay, honeycomb wax, or commercial pruning sealant exhibited 100% survival. Success in adventitious root formation ranged from 75% to 92% among the five successful prophylactic treatments and did not differ among the species. The results indicated that four of the products we evaluated were as effective as commercial pruning sealant for treating the exposed parenchyma on Cycas stem cuttings and enabling asexual propagation success. The candle wax was less effective because it was brittle and cracked to expose the stem’s parenchyma tissue.

scholarly journals A comprehensive phylogeny of auxin homeostasis genes involved in adventitious root formation in carnation stem cuttings

PLoS ONE ◽  
2018 ◽  
Vol 13 (4) ◽  
pp. e0196663 ◽  
Author(s):  
Ana Belén Sánchez-García ◽  
Sergio Ibáñez ◽  
Antonio Cano ◽  
Manuel Acosta ◽  
José Manuel Pérez-Pérez
Keyword(s):  
Adventitious Root ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Stem Cuttings ◽  
Auxin Homeostasis

Polyamines and adventitious root formation in Vitis vinifera L.

OENO One ◽  
2002 ◽  
Vol 36 (2) ◽  
pp. 97
Author(s):  
Laurence Geny ◽  
R. Dalmasso ◽  
Michel Broquedis
Keyword(s):  
Vitis Vinifera ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Free Polyamines ◽  
Conjugated Polyamines ◽  
Mean Size ◽  
The Mean

<p style="text-align: justify;">The effects of polyamines were examined for growth and polyamine contents in cultings, callus and primary adventitious roots of <em>Vitis vinifera</em> L. Variations in free, conjugated and wall-bound polyamines in cuttings were observed during rhizogenesis. The main polyamines in cuttings were conjugated polyamines while in callus and primary adventitious roots they were free polyamines. Exogenous polyamine addition did not modify the total number of roots per cutting but increased the mean size and number of long roots. Moreover, exogenous polyamines increased polyamine levels in callus and roots, particurlarly wall-bound and conjugated polyamines. The involvement of these classes of polyamines in morphogenic processes is discussed.</p>

Sign in / Sign up

Export Citation Format

Share Document