Effect of transplanting dates on yield attributing characters of tomato (Lycopersicon esculentum Mill.) variety

The research was conducted on the field of Chagunarayan municipality (Tathali) from 23rd March to 17th June 2020 to observe the effect date of transplanting on attributing character of tomato Genotype. 23rd March, 6th April, 21st April, and 6th May are the four sowing dates laid out in the RCBD design with three replications. And the data were observed in 10 days intervals for each planting time. The attributing character like plant height, leaf number, no of branches shows positive impact for early shown plant species, and development for later sowing date shows decreasing result. Therefore, the species planted before the planting time is beneficial from an economic point of view where the plant shows a positive growth rate on attributing character, and it can be considered for further research programs as well.

Automated assembly scaffolding elevates a new tomato system for high-throughput genome editing

Advancing crop genomics requires efficient genetic systems enabled by high-quality personalized genome assemblies. Here, we introduce RagTag, a toolset for automating assembly scaffolding and patching, and we establish chromosome-scale reference genomes for the widely used tomato genotype M82 along with Sweet-100, a rapid-cycling genotype that we developed to accelerate functional genomics and genome editing. This work outlines strategies to rapidly expand genetic systems and genomic resources in other plant species.

Genotype and Maturity Stage Affect the Content and Composition of Polyamines in Tomato—Possible Relations to Plant and Human Health

Polyamines (PAs) are molecules affecting several physiological characteristics in all living organisms with cell protective effects, thereby impacting plant and human health. Here, we used HPLC-DAD-ESI-MS to evaluate the content and composition of PAs in eight tomato genotypes over their maturation period, and related the content and composition to other quality traits and possible implications for plant and human health. The tomato genotype, maturity stage and their interactions, significantly affected the content and composition of PAs. Two of the genotypes, ‘Huichol’ and ‘Rio Grande’ showed consistently lower levels of PAs than the other evaluated genotypes. The variation in content and composition of PAs among genotypes was found to vary inconsistently over the maturation period. Putrescine content in the different genotypes either did not vary significantly, increased, or showed the lowest level in the middle of the maturation period, while spermidine content decreased or did not show significant variation. The genotypes ‘HT36’ and ‘HT25’ showed high levels of PAs during red and green maturity stages, respectively, and can thereby be seen as suitable health promoting red and green candidate tomatoes. Depiction of variation of the PAs creates opportunities for breeding and production of health promoting tomato as a food or food additive.

A Novel Protein Hydrolysate-Based Biostimulant Improves Tomato Performances under Drought Stress

Abiotic stresses adversely affect crop production causing yield reductions in important crops, including tomato (Solanum lycopersicum L.). Among the different abiotic stresses, drought is considered to be the most critical one, since limited water availability negatively impacts plant growth and development, especially in arid and semi-arid areas. The aim of this study was to understand how biostimulants may interact with critical physiological response mechanisms in tomato under limited water availability and to define strategies to improve tomato performances under drought stress. We investigated the physiological responses of the tomato genotype ‘E42’ grown in open fields under optimal conditions (100% irrigation) and limited water availability (50% irrigation) treated or not with a novel protein hydrolysate-based biostimulant (CycoFlow, Agriges, BN, Italy). Plants treated with the protein hydrolysate showed a better water status and pollen viability, which also resulted in higher yield under drought stress compared to untreated plants. The treatment with the biostimulant had also an effect on antioxidant contents and activity in leaves and fruits depending on the level of irrigation provided. Altogether, these results indicate that the application of protein hydrolysates on tomato improved plant performances under limited water availability and in different experimental fields.

Evaluation of tomato rootstocks to Ralstonia solanacearum and R. pseudosolanacearum in Mata mesoregion, PE

ABSTRACT Bacterial wilt limits tomato production and resistant rootstocks could be important for the integrated management of the disease. Since there is an interaction between local bacterial strains and tomato genotype, this study aimed to evaluate 14 tomato rootstocks to bacterial wilt in the Mata mesoregion of Pernambuco state, Brazil. The rootstocks reaction to two sequevars of Ralstonia solanacearum and two of R. pseudosolanacearum was evaluated in four experiments carried out in the greenhouse using the completely randomized experimental design, with four replications composed of four plants each. Seven genotypes were selected to evaluate the reaction to bacterial wilt as rootstocks grafting in tomato plants ‘Tomini F1’ in a production area with disease history in the Chã Grande municipality, using randomized block design with four plants per treatment in each block. In the field experiment, disease symptoms were not observed in the grafted plants in ‘Guardião’, ‘Woodstock’, and ‘Yoshimatsu’. Regarding all experiments, ‘Guardião’ and ‘Muralha’ showed the best resistance levels and could be used in the integrated management of bacterial wilt and studied in plant breeding programs.

The intensity of root colonization by phytopathogenic fungus and rhizobacterium depends on the genotype of tomatoes and abscisic acid

The intensity of root colonization by phytopathogenic fungus and rhizobacterium differs depending on the tomato genotype. Inoculation of wild-type tomatoes Ailsa Craig, but not of its ABA deficient mutant flacca, with Novosphingobium sp. P6W inhibits root colonization by Fusarium oxysporum MF-G284.

Tomato Genotype Modulates Selection and Responses to Root Microbiota

Using microbial inoculants to enhance plant health is promising for crop improvement. However, for success, knowledge of how different cultivars within a crop species select and respond to the root microbiome is critical. The aims of this study were to (i) determine the contribution of tomato genotype to the tomato root bacterial microbiome and (ii) investigate whether closely related tomato genotypes differ in their selection of and response to root endophytes. We used 16S ribosomal RNA amplicon sequencing to examine the root bacterial communities of six Solanum lycopersicum (domesticated tomato) and two S. pimpinellifolium (wild tomato) accessions. We found that, across accessions, both the root endosphere and rhizosphere were affected by genotype. Genotype accounted for 10% of the variation in root microbiota. Two bacterial families, Bacillaceae and Rhizobiaceae, were significantly enriched in the root endosphere in at least six of the eight tomato genotypes. To investigate whether closely related tomato genotypes differed in selection of these endosphere-enriched taxa, we profiled the root endosphere of 20 recombinant inbred lines (RILs) derived from two of the genotypes. The abundance of Bacillaceae and Rhizobiaceae isolates varied quantitatively in the root endosphere of the RILs. Inoculation of 16 RILs with a Bacillaceae isolate identified from the root endosphere of field-grown tomato showed that RIL responses, in terms of shoot and root growth, varied from less than 5% growth enhancement to more than 40%. Our data show that tomato genotypes have distinct but overlapping root bacterial microbiomes and respond differently to specific bacterial endophytes.