Studies on in Vitro Culture of the Australian Fan Flower, Scaevola

Plants of genus Scaevola (family, Goodeniaceae), commonly known as “fan flowers,” are mostly endemic to Australia. Commercially popular species are Scaevola aemula, S. albida, S. striata, and S. phlebopetala. These plants are used as ground covers in Australia and as hanging baskets, window boxes, and garden bed plants in Europe and America. Two aspects of in vitro culture of Scaevola are reported here; micropropagation and direct shoot regeneration. A number of commercially available cultivars of S. aemula, S. phlebopetala, S. striata and wild-collected S. phlebopetala, S. glandulifera, S. hookeri, and S. ramonissima were used for micropropagation experiments. Micropropagation medium contained salts, vitamins, L-cysteine, sucrose, and agar. Tissue-cultured shoots were rooted in hormone-free medium. A high survival percentage (>95%) was obtained when plants were transferred to soil under glasshouse conditions. Results on in vitro shoot induction and regeneration response of leaf, stem, root, node, and flower explants of two horticulturally important species of the Australian fan flower, Scaevola aemula and Scaevola striata arealso presented. Of all the explants tested, node explants of these species were the first to respond in tissue culture. Maximum number of shoot induction and regeneration was achieved from node explants of Scaevola aemula and node and stem explants of Scaevola striata. More than 95% of the regenerated shoots were rooted on the medium supplemented with 4 mg/L of IBA. The significance of above findings in assisting breeding program for new horticultural desirable cultivars of Australian fan flowers will be discussed.

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Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d211248 ◽

2020 ◽

Vol 21 (12) ◽

Author(s):

Erma Prihastanti ◽

ENDAH D. HASTUTI ◽

SRI W.A. SUEDY

Keyword(s):

In Vitro Culture ◽

Shoot Growth ◽

Short Communication ◽

Stem Explants ◽

Citrus Reticulata ◽

Culture Methods ◽

Treatment Groups ◽

Physiological Conditions ◽

Growth Differences

Abstract. Prihastanti E, Hastuti ED, Suedy SWA. 2020. Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods. Biodiversitas 21: 5845-5849. Several efforts have been made to preserve Citrus reticulata var. Tawangmangu and Citrus reticulata var. Garut as indigenous Indonesian mandarin cultivars, including in vitro tissue culture methods. This study aimed to determine growth differences of the stem explants of C. reticulata var. Tawangmangu and C. reticulata var. Garut,which planted on the same Murashige and Skoog (MS) media. The treatment groups were derived from different explants, grown in 4 separate culture bottles for 35 days at 25°C. The observed parameters included the percentage of explants indicating callus development browned-colored explants, and the contaminated explants. Among C. reticulata var. Tawangmangu explants, 23.53% indicated callus development, 29.42% were browned-colored explants, and 0% indicated contamination. In contrast, among the C. reticulata var. Garut explants, 0% indicated callus development, 7.14% brown-colored, and 7.14% indicated contamination. The stems explants from C. reticulata var. Tawangmangu showed a tendency to develop calluses, but the explants of C. reticulata var. Garut was able to support the growth of shoots. C. reticulata var. Tawangmangu and Garut mandarin stem explants showed differences of shoot growth because physiological conditions varied according to the variety.

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Micropropagation of Scaevola —Australian native of ornamental horticulture

Australian Journal of Experimental Agriculture ◽

10.1071/ea98020 ◽

1998 ◽

Vol 38 (4) ◽

pp. 399 ◽

Cited By ~ 8

Author(s):

P. L. Bhalla ◽

K. Sweeney

Keyword(s):

Tissue Culture ◽

Butyric Acid ◽

High Survival ◽

Root Initiation ◽

Free Medium ◽

Skoog Medium ◽

Survival Percentage ◽

Murashige And Skoog Medium ◽

Ornamental Horticulture

Summary. A number of commercially available cultivars of Scaevola aemula, S. albida, S. phlebopetala, S. striata and material collected from the wild of S. glandulifera, S. hookeri and S. ramonissima were successfully propagated by tissue culture. Shoot segments 3–4 cm in length were multiplied in Murashige and Skoog medium without hormones. Addition of 25–150 µmol kinetin/L in the micropropagation medium of S. aemula and S. phlebopetala resulted in the formation of deformed shoots. Tissue cultured shoots rooted in hormone-free medium in 4–6 weeks. Indole-3-butyric acid (10–20 µmol/L) had an effect on rate of root initiation of S. phlebopetala but not on percentage of rooting. A high survival percentage (>95%) was obtained when plants were transferred to soil under glasshouse conditions indicating that micropropagation of Scaevola is feasible.

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Regional Substrates of the Brazilian Northeast on Acclimatization and Development Morphological of Minirose

Journal of Agricultural Science ◽

10.5539/jas.v10n6p341 ◽

2018 ◽

Vol 10 (6) ◽

pp. 341

Author(s):

David Correia dos Anjos ◽

Gabrielen De Maria Gomes Dias ◽

Fernando Felipe Ferreyra Hernandez ◽

Josefa Diva Nogueira Diniz

Keyword(s):

In Vitro Culture ◽

Rice Husk ◽

Dry Matter ◽

Good Choice ◽

Morphological Development ◽

Shoot Height ◽

Rosa Chinensis ◽

Survival Percentage ◽

Brazilian Northeast

The transition from heterotrophism to autotrophism in acclimatization is a delicate process for most species. The objective of this work was to evaluate combinations of regional substrates of the Brazilian Northeast in the acclimatization and morphological development of minirose (Rosa chinensis ‘Minima’) seedlings. The experiment was carried out in a greenhouse using seedlings with 30 days of in vitro culture. The treatments were constituted by: 1) dry coconut powder; 2) 75% dry coconut powder + 25% rice husk; 3) 50% dry coconut powder + 50% rice husk; 4) 75% dry coconut powder + 25% charcoal rice husk; 5) 50% dry coconut powder + 50% carbonized rice husk; 6) 100% vermiculite; 7) 50% vermiculite + 50% dry coconut powder; 8) commercial substrate; 9) 75% sand + 25% dry coconut powder and 10) 50% sand + 50% dry coconut powder. After 21 days, the following characteristics were evaluated: survival percentage, leaf number, shoot height, dry matter of shoot and root and total dry matter. The regional materials tested showed a good choice of substrates to be used in the acclimatization of minirose seedlings. The vermiculite presented the worst results in the characteristics evaluated. Dry coconut powder as substrate should be used in mixtures with other materials to provide better acclimatization conditions. The use of 50% dry coconut powder + 50% rice husk provided a best development and growth of minirose seedlings (Rosa chinensis ‘Minima’) than other tested substrates

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A Microarray Analysis of the Effects of Serum-free Medium on Gene Expression Changes in Human Mesenchymal Stem Cells during the in Vitro Culture

YAKUGAKU ZASSHI ◽

10.1248/yakushi.130.1387 ◽

2010 ◽

Vol 130 (10) ◽

pp. 1387-1393 ◽

Cited By ~ 5

Author(s):

Rumi SAWADA ◽

Takashi YAMADA ◽

Toshie TSUCHIYA ◽

Atsuko MATSUOKA

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

In Vitro Culture ◽

Microarray Analysis ◽

Human Mesenchymal Stem Cells ◽

Serum Free Medium ◽

Free Medium ◽

Serum Free

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An efficient in vitro propagation protocol for snowdrop anemone (Anemone sylvestris L.)

Horticultural Science ◽

10.17221/266/2015-hortsci ◽

2017 ◽

Vol 44 (No. 4) ◽

pp. 186-194 ◽

Cited By ~ 3

Author(s):

Jana Šedivá ◽

Pavla Zahumenická ◽

Eloy Fernández Cusimamani

Keyword(s):

Plant Growth ◽

In Vitro Propagation ◽

Shoot Multiplication ◽

Growth Characteristics ◽

Root Induction ◽

Shoot Induction ◽

Free Medium ◽

In Vitro Production ◽

Vitro Production

This study investigated in vitro production of diploid (AS2) and tetraploid (AS4) cytotypes of snowdrop anemone. The effect of plant growth regulators (PGRs) on in vitro shoot multiplication and rooting was investigated. The effect of activated charcoal (AC) on root induction was also studied. Ploidy level affected growth characteristics during multiplication and rooting. Shoot induction in AS4 was higher on medium supplemented with cytokinin (3.2–3.6), while the AS2 clone formed the most shoots on PGR-free medium (3.6). The highest rooting percentage was achieved on PGR-free medium in both genotypes (AS2 clone, 100% and AS4 clone, 93.3%). The addition of AC to the PGR media largely increased root induction and root length. Rooted plantlets were successfully acclimatised in the greenhouse with 100% survival. Thus, the described micropropagation protocol represents a rapid and effective in vitro propagation method for utilisation in horticulture and conservation programmes of snowdrop anemone.

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Use of plasma-free medium for the in vitro culture of human lymphocytes

Cancer ◽

10.1002/1097-0142(197304)31:4<882::aid-cncr2820310418>3.0.co;2-4 ◽

1973 ◽

Vol 31 (4) ◽

pp. 882-888 ◽

Cited By ~ 4

Author(s):

R. C. Bagai ◽

T. H. M. Stewart ◽

J. E. Harris

Keyword(s):

In Vitro Culture ◽

Human Lymphocytes ◽

Free Medium

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In Vitro Culture of Heuchera villosa ‘Caramel’

HortScience ◽

10.21273/hortsci11340-16 ◽

2017 ◽

Vol 52 (4) ◽

pp. 622-624 ◽

Cited By ~ 2

Author(s):

Hua Q. Zhao ◽

Qing H. He ◽

Li L. Song ◽

Mei F. Hou ◽

Zhi G. Zhang

Keyword(s):

Acetic Acid ◽

In Vitro Culture ◽

Butyric Acid ◽

Shoot Regeneration ◽

Ms Medium ◽

Shoot Induction ◽

Induction Rate ◽

Regenerated Plantlets ◽

Indole 3 Acetic Acid

The procedure for Heuchera villosa ‘Caramel’ propagation was investigated, which involves shoot regeneration, rooting of regenerated shoots, and acclimation of regenerated plantlets. Petioles, as explants, were cultured on MS medium supplemented with 1-naphthylacetic acid (NAA), benzylaminopurine (BA), thidiazuron (TDZ) and callus formed on all media. Shoots were observed to proliferate from callus on media with BA and NAA, whereas no shoots regenerated on media with TDZ and NAA. On media containing 0.5 or 1.0 mg·L−1 BA in combination with NAA, the regenerated shoots showed severe hyperhydricity, whereas on media containing 0.1 mg·L−1 BA in combination with NAA, the regenerated shoots grew normally. The highest shoot induction rate, 90.6%, was obtained on media containing 0.1 mg·L−1 BA and 0.01 mg·L−1 NAA. The effects of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and NAA on rooting of H. villosa ‘Caramel’ was explored. The highest rooting rate (95%) was obtained on 1/2 MS medium containing 0.2 mg·L−1 NAA. In the subsequent acclimation experiments, about 85% of rooted plantlets survived and grew normally.

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Improvement of In Vitro Proliferation and Elongation of Habanero Pepper Shoots (Capsicum chinense Jacq.) by Temporary Immersion

HortScience ◽

10.21273/hortsci.45.7.1093 ◽

2010 ◽

Vol 45 (7) ◽

pp. 1093-1098 ◽

Cited By ~ 9

Author(s):

Jericó J. Bello-Bello ◽

Adriana Canto-Flick ◽

Eduardo Balam-Uc ◽

Eunice Gómez-Uc ◽

Manuel L. Robert ◽

...

Keyword(s):

Shoot Elongation ◽

Nodal Segments ◽

High Survival ◽

Shoot Induction ◽

Capsicum Chinense ◽

Temporary Immersion Bioreactor ◽

In Vitro Proliferation ◽

Temporary Immersion ◽

Habanero Pepper

This article describes the performance of nodal segments from Habanero pepper (Capsicum. chinense) during shoot induction and elongation under different semisolid and liquid culture conditions with various degrees of ventilation in which they were exposed to different levels of immersion and growth regulators. The ethylene content in non-ventilated containers, the age of the explant donor plants as well as the effect of thidiazuron and paclobutrazol on shoot induction and of gibberellic acid and AgNO3 on shoot elongation were also evaluated. A temporary immersion bioreactor (BioMINT™) was used for the multiplication and elongation of isolated shoots with very good results. We report an efficient protocol for the in vitro propagation of Habanero pepper that produces plants with a high survival rate when transplanted to soil.

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