scholarly journals Regional Substrates of the Brazilian Northeast on Acclimatization and Development Morphological of Minirose

2018 ◽  
Vol 10 (6) ◽  
pp. 341
Author(s):  
David Correia dos Anjos ◽  
Gabrielen De Maria Gomes Dias ◽  
Fernando Felipe Ferreyra Hernandez ◽  
Josefa Diva Nogueira Diniz
Keyword(s):  
In Vitro Culture ◽  
Rice Husk ◽  
Dry Matter ◽  
Good Choice ◽  
Morphological Development ◽  
Shoot Height ◽  
Rosa Chinensis ◽  
Survival Percentage ◽  
Brazilian Northeast

The transition from heterotrophism to autotrophism in acclimatization is a delicate process for most species. The objective of this work was to evaluate combinations of regional substrates of the Brazilian Northeast in the acclimatization and morphological development of minirose (Rosa chinensis ‘Minima’) seedlings. The experiment was carried out in a greenhouse using seedlings with 30 days of in vitro culture. The treatments were constituted by: 1) dry coconut powder; 2) 75% dry coconut powder + 25% rice husk; 3) 50% dry coconut powder + 50% rice husk; 4) 75% dry coconut powder + 25% charcoal rice husk; 5) 50% dry coconut powder + 50% carbonized rice husk; 6) 100% vermiculite; 7) 50% vermiculite + 50% dry coconut powder; 8) commercial substrate; 9) 75% sand + 25% dry coconut powder and 10) 50% sand + 50% dry coconut powder. After 21 days, the following characteristics were evaluated: survival percentage, leaf number, shoot height, dry matter of shoot and root and total dry matter. The regional materials tested showed a good choice of substrates to be used in the acclimatization of minirose seedlings. The vermiculite presented the worst results in the characteristics evaluated. Dry coconut powder as substrate should be used in mixtures with other materials to provide better acclimatization conditions. The use of 50% dry coconut powder + 50% rice husk provided a best development and growth of minirose seedlings (Rosa chinensis ‘Minima’) than other tested substrates

Digestibility and chemical composition of fractions of lucerne during spring and summer

1970 ◽  
Vol 75 (2) ◽  
pp. 213-222 ◽  
Author(s):  
K. R. Christian ◽  
D. B. Jones ◽  
M. Freer
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Dry Weight ◽  
Chemical Components ◽  
Shoot Height ◽  
Time Of Year ◽  
Highly Correlated ◽  
Leaf Composition

SUMMARYBulk harvests of primary growth were made at intervals during the spring and summer of 2 consecutive years from an ungrazed area of lucerne (Medicago sativa) and the digestibility by sheep was measured. At the same times, shoots of known length representative of the sward were collected, divided into 7·5 cm lengths, and separated into leaf and stem for the determination of ash, nitrogen, cellulose, and in vitro organic matter digestibility.Digestibility and leaf: stem ratio of the bulk harvests decreased and dry matter increased as the season advanced. Leaf composition changed little, but stem increased in lignin and decreased in ash and in vitro digestibility. In vitro digestibility and leaf: stem ratio were highly correlated with sheep digestibility.Chemical composition of shoots of known heights was similar to that of the bulk harvests. Leaf weights/shoot tended to increase linearly with height of shoot, whereas stem increased nearly as the square of the height. Dry matter of leaf and stem were inversely related to leaf:stem ratio. Stem digestibility decreased with leaf:stem ratio and with increase in shoot height.Chemical components of shoot fractions were associated with each other and were largely dependent on the distance from the top of the shoot at which they had been taken, irrespective of shoot height or time of year. Bottom stems had lower ash, nitrogen and digestibility but higher cellulose, dry weight and dry matter than top stems, whose composition approached that of leaves. Leaf fractions showed little consistent trend with position on the shoot.Some of the difficulties in studying changes in plant composition under field conditions are discussed.

scholarly journals Acclimatization and growth of ornamental pineapple seedlings under organic substrates

2017 ◽  
Vol 23 (3) ◽  
pp. 257
Author(s):  
Ronan Carlos Colombo ◽  
Vanessa Favetta ◽  
Maria Aparecida da Cruz ◽  
Deived Uilian de Carvalho ◽  
Sergio Ruffo Roberto ◽  
...  
Keyword(s):  
Rice Husk ◽  
Pine Bark ◽  
Ananas Comosus ◽  
Organic Substrates ◽  
Seedling Mortality ◽  
Survival Percentage ◽  
Number Of Leaves ◽  
Physical And Chemical ◽  
The Ideal

The in vitro propagation techniques are commonly used to produce ornamental pineapple seedlings in commercial scale, aiming to attend the growers with genetic and sanitary quality seedlings. However, the choice of the ideal substrate is essential for the acclimatization and growth stage of the seedlings propagated by this technique, since some substrates can increase the seedling mortality and/or limit the seedling growth due to its physical and chemical characteristics. Thus, the aim of this study was to evaluate the acclimatization of ornamental pineapple [Ananas comosus (L.) Merr. var. ananassoides (Baker) Coppens & Leal] on different substrates. Seedlings with approximately seven centimeters, obtained from in vitro culture, were transplanted into styrofoam trays filled with the following substrates: sphagnum; semi-composed pine bark; carbonized rice husk; sphagnum + semicomposed pine bark; sphagnum + carbonized rice husk; and semi-composed pine bark + carbonized rice husk. Each treatment was replicated five times using 10 plants. At 180 days, there were evaluated the following variables: survival percentage, plant height, number of leaves, leaf area, largest root length, and shoot and root dry matter. The substrate semi-composed pine bark + carbonized rice husk presented the lowest mean (62%) for survival percentage. The semi-composed pine bark and semi-composed pine bark + carbonized rice husk treatments presented significant increments in some evaluated biometric characteristics. The semi-composed pine bark is the most favorable substrate for the A. comosus var. ananassoids acclimatization.

scholarly journals In Vitro Ruminal Fermentation and Degradability of Rice Husk on Rice Bran Substitution

2020 ◽  
Vol 24 (1) ◽  
pp. 50
Author(s):  
Rusli Fidriyanto ◽  
Roni Ridwan ◽  
Wulansih Dwi Astuti ◽  
Rohmatussolihat Rohmatussolihat ◽  
Nurul Fitri Sari ◽  
...  
Keyword(s):  
Organic Matter ◽  
Rice Husk ◽  
Rice Bran ◽  
Dry Matter ◽  
Acid Level ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Fatty Acid Production ◽  
Dry Matter Digestibility

Rice bran is a by-product of the rice milling process and has been well used as livestock feed. Rice bran is often adulterated with rice husk. The objective of this study was to evaluate the in vitro ruminal fermentation characteristics of rice bran with various compositions of rice husk and assess the relationship between rice husk addition and rice bran quality. The experiment was arranged in a completely randomized design with rice husk addition as a factor and three replications. Data of proximate value, gas production, ruminal degradability, and volatile fatty acid production were analyzed by analysis of variance. Moreover, significant effects of each treatment in the in vitro fermentation were further analyzed by Duncan's multiple range test (P<0.05). It was shown that the addition of rice husk to rice bran could increase acetic acid level, but it reduced potential gas production, gas production rate, organic matter and dry matter digestibility, and propionic acid level. Interestingly, the linear regression of dry matter digestibility, organic matter digestibility, and potential gas production showed the high adjusted R2 values. Moreover, this study also revealed that 10% of rice husk substitution on rice bran could significantly reduce the dry matter digestibility.

scholarly journals IMPACT OF VARIOUS 2, 4-D CONCENTRATIONS AND DIFFERENT VITAMIN MIXTURES ON in vitro CULTURE OF COCONUT (Cocos nucifera L.) BY UTILIZING SEEDLINGS SHOOT TIP

2020 ◽  
Vol 19 (2) ◽  
pp. 135-145
Author(s):  
Sherif Fathy ElGioushy ◽  
Liu Rui ◽  
Haikuo Fan
Keyword(s):  
In Vitro Culture ◽  
Cocos Nucifera ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Survival Percentage ◽  
High Concentrations ◽  
Cocos Nucifera L ◽  
The Impact ◽  
Vitamin Mixture

In this experiment, seedlings as sources for shoot tips was assembled from the coconut research institute (CRI) of Chinese Academy of tropical agricultural sciences germplasm, Wenchang, Hainan, China. The shoot tips were cultured on Y3 medium to study the impact of ten concentrations of 2, 4-D and three vitamin mixtures on survival, death and development % on in vitro culture establishment stage of coconut (Cocos nucifera L.). The obtained results revealed that all 2,4-D concentrations 4,7, 75, 100, 120 and 140 mg/L + any vitamin mixture did not give any development and high concentrations of 2, 4-D increased the death %. In any case, it can be concluded that the rate of differences in survival, death and development percentages exhibited by 2, 4-D concentrations was more pronounced than the analogous ones resulted by vitamin mixtures. At any rate, utilizing of (2, 4-D at concentration 25 mg/L+ vitamin mixture 1 or 2) exhibited the greatest values of survival percentage and reduced death percentage. Just as, increased the percentages of development in in vitro culture establishment stage of coconut (Cocos nucifera L.) through seedlings shoot tip.

scholarly journals Studies on in Vitro Culture of the Australian Fan Flower, Scaevola

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 548B-548
Author(s):  
Prem L. Bhalla ◽  
Katherine Tozer
Keyword(s):  
In Vitro Culture ◽  
Stem Explants ◽  
High Survival ◽  
Shoot Induction ◽  
Free Medium ◽  
Direct Shoot Regeneration ◽  
Important Species ◽  
Survival Percentage ◽  
Regeneration Response

Plants of genus Scaevola (family, Goodeniaceae), commonly known as “fan flowers,” are mostly endemic to Australia. Commercially popular species are Scaevola aemula, S. albida, S. striata, and S. phlebopetala. These plants are used as ground covers in Australia and as hanging baskets, window boxes, and garden bed plants in Europe and America. Two aspects of in vitro culture of Scaevola are reported here; micropropagation and direct shoot regeneration. A number of commercially available cultivars of S. aemula, S. phlebopetala, S. striata and wild-collected S. phlebopetala, S. glandulifera, S. hookeri, and S. ramonissima were used for micropropagation experiments. Micropropagation medium contained salts, vitamins, L-cysteine, sucrose, and agar. Tissue-cultured shoots were rooted in hormone-free medium. A high survival percentage (>95%) was obtained when plants were transferred to soil under glasshouse conditions. Results on in vitro shoot induction and regeneration response of leaf, stem, root, node, and flower explants of two horticulturally important species of the Australian fan flower, Scaevola aemula and Scaevola striata arealso presented. Of all the explants tested, node explants of these species were the first to respond in tissue culture. Maximum number of shoot induction and regeneration was achieved from node explants of Scaevola aemula and node and stem explants of Scaevola striata. More than 95% of the regenerated shoots were rooted on the medium supplemented with 4 mg/L of IBA. The significance of above findings in assisting breeding program for new horticultural desirable cultivars of Australian fan flowers will be discussed.

scholarly journals BIOBRAS-16 IMPROVES THE ACCLIMATIZATION OF SPANISH CARNATION (DIANTHUS CARYOPHYLLUS L.) PLANTS OBTAINED IN VITRO

2015 ◽  
Vol 5 (3) ◽  
pp. 741-747
Author(s):  
Yanelis Castilla Valdés ◽  
María Esther González Vega
Keyword(s):  
In Vitro Culture ◽  
Dianthus Caryophyllus ◽  
Survival Percentage ◽  
Controlled Conditions ◽  
The Moment

The Spanish carnation (Dianthus caryophyllus L.) is one of the most commercialized flowers worldwide. Its accelerated propagation is possible due to the utilization of micropropagation methods, especially if combined with the employment of different bioproducts, between them the brassinosteroid analogous, known as Biobras-16. However, up to the moment, there is a lack of references about the acclimatization of Spanish carnation plants, micropropagated with this brassinosteroid analogue. For this reason, in the present work we proposed the aim of determine the survival of plants of Spanish carnation acclimatized with the employment of Biobras-16. The plants were obtained from meristems, cultured in medium with different concentrations of Biobras-16 (10-1; 10-2 and 10-3 mg·L-1), and from them, two propagations were made by cuttings, without the addition of Biobras-16 but respecting the treatments of origin of the plants. The plants obtained from the second propagation, were acclimatized under semi controlled conditions, using two assays: the first one, without treatment with Biobras-16, and the second one, with the application to the leaves of a solution with the same concentrations of Biobras-16 used in each treatment of the meristems culture. In general, in the control treatments was obtained an intermediate survival of the plants of Spanish carnation, confirming it is a bounding and transcendental stage for the successful culmination of the micropropagation process. In the first assay, the plants survival overcame significantly to the control in the treatmentwhich employed 10-3 mg·L-1 of Biobras-16 during the micropropagation. In the second assay, in the treatments where the plants were sprayed with 10-1 and 10-3 mg·L-1of brassinosteroid analogue, were obtained higher survival percentages than the obtained in the first assay. It was appreciated the feasibility of the use of the Biobras-16, because its use in the in vitro culture and its spraying during the acclimatization phase of the Spanish carnation vitroplants, it is capable to produce increases in the survival percentage between 12,5% and 17%, which increment the efficiency of the micropropagation process.  

235 PROGESTERONE AFFECTS THE MESSENGER RNA EXPRESSION OF IN VITRO-PRODUCED BOVINE EMBRYOS

2013 ◽  
Vol 25 (1) ◽  
pp. 265
Author(s):  
K. Knauer ◽  
H. Stinshoff ◽  
S. Wilkening ◽  
C. Wrenzycki
Keyword(s):  
In Vitro Culture ◽  
Messenger Rna ◽  
Blastocyst Stage ◽  
Developmental Competence ◽  
Morphological Development ◽  
Bovine Embryo ◽  
Development Rates ◽  
Vitro Model

It is known that the progesterone (P4) provided by the corpus luteum is essential for the maintenance of pregnancy. It has been suggested that supplying external P4 in vivo is beneficial to the establishment and upkeep of pregnancy. The aim of the present study was to assess the effects of supplementation with different concentrations of P4 on either of 2 days of in vitro culture (IVC) on early bovine embryo development in an in vitro model. A total of 5073 cumulus–oocyte complexes were matured and fertilized in vitro. Before culture, they were collected in groups of 30 and allocated to 1 of 9 groups. The groups were supplemented with 10, 20, or 100 ng of P4 on Days 4 or 5 of IVC (IVF = Day 0). Alcohol (ETOH) was used as the solvent, so 8 µL of ETOH was used per supplementation. Therefore, two additional groups were supplemented with only ETOH on Day 4 or 5 of IVC. The presumptive zygotes allocated to group 9 were not supplemented. A culture system without oil overlay was used to prevent the lipophilic P4 from moving into the oil. Embryo cleavage and development rates were determined solely on Day 8 of IVC. Single expanded blastocysts were stored at –80°C for RT-qPCR. Subsequently, the relative amounts of six developmentally important gene transcripts (IGF1R, SLC2A1, HSD3B1, IFNT, PGRMC1, and PGRMC2) were analysed in single embryos of all groups. Statistical analysis was performed using one-way and two-way ANOVA, and the level of significance was set at P ≤ 0.05. Cleavage and development rates did not differ among groups (see Table 1). The relative abundance of IGF1R, SLC2A1, PGRMC1, and PGRMC2 was not affected by either the concentration or the timing of P4 supplementation. Nevertheless, there was a statistically significant interaction between the day of treatment and the concentration used for the expression of HSD3B1 mRNA. When 20 ng of P4 was added on Day 5 of IVC, significantly more HSD3B1 transcripts were detected than if 10 ng, 100 ng, or ETOH alone was added. The expression of IFNT was not affected by the day of supplementation, only by the concentration used. Thus, supplementation with 20 ng of P4 resulted in a significantly higher level of transcripts than when 10 ng or ETOH was supplemented. The results indicate that the amount of P4 present during early embryonic development and the timing of its presence had an impact on molecular developmental competence. However, no effects concerning morphological development up to the blastocyst stage could be detected. Table 1.Cleavage and development rates (± SEM) of embryos supplemented with 10, 20, or 100 ng on Day 4 or 5 of in vitro culture (P ≥ 0.05) The financial support of the FBF e.V. is acknowledged.

scholarly journals Multiplicação in vitro de Codonanthe devosiana

2018 ◽  
Vol 24 (1) ◽  
pp. 58-62
Author(s):  
Aquélis Armiliato Emer ◽  
Mara Cíntia Winhelmann ◽  
Gislaine Taís Grzeça ◽  
Claudimar Sidnei Fior ◽  
Gilmar Schafer
Keyword(s):  
Dry Matter ◽  
Root Formation ◽  
Shoot Height ◽  
The Family ◽  
Multiple Buds ◽  
Number Of Shoots

Codonanthe devosiana is a species endemic to Brazil belonging to the family Gesneriaceae. It features ornamental potential for use in hanging pots and shaded environments. The aim is to evaluate the effects of the combination of different concentrations of BAP and GA3 on the multiplication and development of in vitro explants of C. devosiana. We used MS media supplemented with concentrations of 0.0, 0.2 and 0.4 mg L-1 of BAP and 0.0, 0.25 and 0.50 mg L-1 of GA3 . The number, bud dry matter and height, callus dry matter and presence of roots were evaluated in agglomerates with multiple buds. The interaction between BAP and GA3 concentrations was verified for number of shoots, shoot dry matter and callus dry matter. For shoot height and root formation, only BAP concentrations were observed. The highest number of shoots (4.8) occurred when combined with the concentration of BAP 0.2 mg L-1 and 0.5 mg L-1 GA3 . The lowest shoot height was observed without the use of BAP, as well as the greatest rooting. It is concluded that the combination of 0.2 mg L-1 of BAP with 0.5 mg L-1 of GA3 allows for a more efficient multiplication of C. devosiana explants

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