Unknown Cultivars of Cold-hardy Grape Can Be Successfully Identified by Their Simples Sequence Repeat (SSR) Fingerprints

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 516d-517
Author(s):  
Warren F. Lamboy ◽  
Christopher A. Alpha ◽  
David V. Peterson
Keyword(s):  
New York ◽  
Practical Method ◽  
Pinot Noir ◽  
Sequence Repeat ◽  
Blind Test ◽  
Commercially Important ◽  
Cold Hardy ◽  
Grape Cultivars ◽  
Ssr Fingerprinting ◽  
Cool Climate

Simple sequence repeat DNA fragments (SSRs) have been suggested as the method of choice for DNA fingerprinting of grape cultivars. Nevertheless, the use of SSRs as a practical fingerprinting method is not without its pitfalls. For example, when the polymerase chain reaction is used to amplify SSR sequences, potentially confusing “stutter” bands may occur, or there may be non-template directed addition of an “A” to the end of synthesized fragments, or other artifactual amplification products may be produced. Since we would like to fingerprint our entire cold-hardy grape collection of ≈1300 cultivars, we decided to conduct a blind test to determine if SSR fingerprinting actually would be practical in our circumstances. First, SSR fingerprints were established for 45 commercially important cool-climate grape cultivars, the known standards. Then, SSR fingerprints were produced for 44 “unknown” cultivars grown in the Finger Lakes Region of New York. The identities of these were known only to the third author. To independently identity these “unknowns,” their fingerprints were compared to those of the known standards. By this means, 42 of the 44 “unknowns” were immediately correctly identified. The identity of one of the two remaining unknowns was truly not known to the vineyard owner; it was identified as Cabernet Franc, a grape commonly grown in the region. The final “unknown” was a plant of Pinot Blanc, whose fingerprint matched those of both the known and the unknown Pinot Gris and Pinot Noir plants, but did not match that of the Pinot Blanc plants used as standards. This was surprising, since all three Pinot's varieties are simply fruit color mutants of the same genotype. Further investigation revealed that the known plants of “Pinot Blanc” had been misidentified, and actually were the cultivar Melon. Thus, identification of the “unknown” Pinot Blanc as Pinot Noir or Pinot Gris was correct, as were the identifications of the 43 other `unknowns.” This study confirmed that SSR fingerprinting is a practical method for identifying cool-climate grape cultivars.

scholarly journals Viticultural Performance of Hybrids and Vitis vinifera Varieties Established in Annapolis Valley (Nova Scotia)

Horticulturae ◽  
2021 ◽  
Vol 7 (9) ◽  
pp. 291
Author(s):  
Francisco Diez-Zamudio ◽  
Rodrigo Laytte ◽  
Cecilia Grallert ◽  
Nedret Neslihan Ivit ◽  
Gastón Gutiérrez-Gamboa
Keyword(s):  
New York ◽  
Nova Scotia ◽  
Vitis Vinifera ◽  
Scientific Evidence ◽  
Pinot Noir ◽  
Good Tolerance ◽  
Spring Frost ◽  
Local Experience ◽  
Cold Hardy ◽  
Annapolis Valley

(1) Background: Cold-hardy interspecific hybrid grapes (CIHG) are well adapted to the Annapolis Valley edaphoclimatic conditions. The main characteristics of CIHG are the high bud hardiness tolerance to winter frost, the short growing cycle, and the good tolerance to cryptogamic diseases. Based on local experience, the Vitis vinifera varieties should be grown in the warmest areas of the Annapolis Valley (Nova Scotia, Canada). Despite this, there is little scientific evidence that shows the viticultural behavior of these varieties under the edaphoclimatic conditions of this valley. (2) Methods: Thus, the aim of this research was to evaluate the viticultural behavior of two CIHG (L’Acadie and New York Muscat) and three V. vinifera varieties (Chardonnay, Riesling, and Pinot Noir) growing in the Annapolis Valley over three consecutive seasons. (3) Results: The coldest season (2019) produced a delay in grapevine phenology of at least 18 days for budburst compared to the warm seasons (2017 and 2018). In addition, in the coldest season from budburst to bloom the duration decreased compared to the rest of the seasons. The main phenological stages started earlier in L’Acadie than in the V. vinifera varieties. L’Acadie presented lower N petiole content than the V. vinifera varieties, which conditioned shoot growth in the studied seasons. CIHG presented low B petiole levels and produced musts with low malic acid content, while V. vinifera varieties produced musts with high N content. L’Acadie was the only variety that could bud out, and differentially produced fruit after the spring frost of −2 to −3 °C for 2 h in 2018 in this trial. (4) Conclusions: L’Acadie, and to a lesser extent, Riesling, hold an interesting adaptation to the edaphoclimatic conditions of the Annapolis Valley.

scholarly journals Genotyping of Peach and Nectarine Cultivars with SSR and SRAP Molecular Markers

2004 ◽  
Vol 129 (2) ◽  
pp. 204-210 ◽  
Author(s):  
Riaz Ahmad ◽  
Dan Potter ◽  
Stephen M. Southwick
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Sweet Cherry ◽  
Prunus Persica ◽  
Sequence Repeat ◽  
Srap Markers ◽  
Upgma Cluster Analysis ◽  
Commercially Important ◽  
Simple Sequence

Simple sequence repeat (SSR) and sequence related amplified polymorphism (SRAP) molecular markers were evaluated for detecting intraspecific variation in 38 commercially important peach and nectarine (Prunus persica) cultivars. Out of the 20 SSR primer pairs 17 were previously developed in sweet cherry and three in peach. The number of putative alleles revealed by SSR primer pairs ranged from one to five showing a low level of genetic variability among these cultivars. The average number of alleles per locus was 2.2. About 76% of cherry primers produced amplification products in peach and nectarine, showing a congeneric relationship within Prunus species. Only nine cultivars out of the 38 cultivars could be uniquely identified by the SSR markers. For SRAP, the number of fragments produced was highly variable, ranging from 10 to 33 with an average of 21.8 per primer combination. Ten primer combinations resulted in 49 polymorphic fragments in this closely related set of peaches and nectarines. Thirty out of the 38 peach and nectarine cultivars were identified by unique SRAP fingerprints. UPGMA Cluster analysis based on the SSR and SRAP polymorphic fragments was performed; the relationships inferred are discussed with reference to the pomological characteristics and pedigree of these cultivars. The results indicated that SSR and SRAP markers can be used to distinguish the genetically very close peach and nectarine cultivars as a complement to traditional pomological studies. However, for fingerprinting, SRAP markers appear to be much more effective, quicker and less expensive to develop than are SSR markers.

scholarly journals Chemical markers in the aroma profiles of South Moravian red wine distillates

2012 ◽  
Vol 30 (No. 4) ◽  
pp. 369-376 ◽  
Author(s):  
L. Veverka ◽  
M. Jelínková ◽  
K. Hron ◽  
J. Balík ◽  
J. Stávek ◽  
...  
Keyword(s):  
Red Wine ◽  
Pinot Noir ◽  
The Other ◽  
Cabernet Sauvignon ◽  
Chemical Markers ◽  
The Czech Republic ◽  
Linalool Oxide ◽  
South Moravia ◽  
Log Ratio ◽  
Grape Cultivars

HSSPME-GC/MS method was used to investigate the volatile compounds responsible for varietal character in the aroma of wine distillates made from 16 different red wine grape cultivars: Andre, Blue Frankish, Merlot, Cabernet Moravia, Rubinet, Pinot Noir, Ariana, Alibernet, Laurot, Dornfelder, Blauer Portugieser, Agni, Neronet, Zweigeltrebe, Cabernet Sauvignon, and Domina. The grapes were all grown in the same vineyard in South Moravia, an important viticultural region in the south of the Czech Republic bordering Austria. The isometric log-ratio transformation was used to compute variances prior to statistical analysis, and a compositional biplot was used to interpret the data and identify the main chemical markers. A comparison of the key terpenoids present in the aroma profiles indicated that these were consistent with the known relationships between the cultivars based on their parentage. There were similarities in the terpenoid elements of the aroma profiles of Blue Frankish and its relatives Andre, Laurot, Agni, and Zweigeltrebe, which are dominated by (Z)-linalool oxide, linalool, isoborneol, terpinen-4-ol and α-terpineol. On the other hand, the aroma profiles of Pinot Noir, Blauer Portugieser, Cabernet Sauvignon and their related hybrids are dominated by o-cymene, limonene, (E)-sabinyl acetate, and (E)-calamenene.  

Nitrogen use efficiency, partitioning, and storage in cool climate potted Pinot Noir vines

2022 ◽  
Vol 291 ◽  
pp. 110603
Author(s):  
Harriet V. Walker ◽  
Nigel D. Swarts ◽  
Joanna E. Jones ◽  
Fiona Kerslake
Keyword(s):  
Nitrogen Use Efficiency ◽  
Pinot Noir ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
And Storage ◽  
Cool Climate

Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.)

Genome ◽  
1996 ◽  
Vol 39 (4) ◽  
pp. 628-633 ◽  
Author(s):  
J. E. Bowers ◽  
G. S. Dangl ◽  
R. Vignani ◽  
C. P. Meredith
Keyword(s):  
Vitis Vinifera ◽  
Silver Staining ◽  
Simple Sequence Repeat ◽  
Pinot Noir ◽  
Sequence Repeat ◽  
Wine Grapes ◽  
Table Grapes ◽  
Isolation And Characterization ◽  
Ssr Loci ◽  
Simple Sequence

Four new simple sequence repeat (SSR) loci (designated VVMD5, VVMD6, VVMD7, and VVMD8) were characterized in grape and analyzed by silver staining in 77 cultivars of Vitis vinifera. Amplification products ranged in size from 141 to 263 base pairs (bp). The number of alleles observed per locus ranged from 5 to 11 and the number of diploid genotypes per locus ranged from 13 to 27. At each locus at least 75% of the cultivars were heterozygous. Alleles differing in length by only 1 bp could be distinguished by silver staining, and size estimates were within 1 or 2 bp, depending on the locus, of those obtained by fluorescence detection at previously reported loci. Allele frequencies were generally similar in wine grapes and table grapes, with some exceptions. Some alleles were found only in one of the two groups of cultivars. All 77 cultivars were distinguished by the four loci with the exception of four wine grapes considered to be somatic variants of the same cultivar, 'Pinot noir', 'Pinot gris', 'Pinot blanc', and 'Meunier'; two table grapes that are known to be synonymous, 'Keshmesh' and 'Thompson Seedless'; and three table grapes, 'Dattier', 'Rhazaki Arhanon', and 'Markandi', the first two of which have been suggested to be synonymous. Although the high polymorphism at grape SSR loci suggests that very few loci would theoretically be needed to separate all cultivars, the economic and legal significance of grape variety identification requires the increased resolution that can be provided by a larger number of loci. The ease with which SSR markers and data can be shared internationally should encourage their broad use, which will in turn increase the power of these markers for both identification and genetic analysis of grape. Key words : grape, Vitis, microsatellite, simple sequence repeat, DNA typing, identification.

scholarly journals MOLECULAR MARKERS FOR CHARACTERIZATION OF GRAPE CULTIVARS II

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 660f-660
Author(s):  
Y. Gogorcena ◽  
S. Arulsekar ◽  
D.E. Parfitt
Keyword(s):  
Molecular Markers ◽  
Extraction Method ◽  
Rapd Markers ◽  
Pinot Noir ◽  
Specific Reference ◽  
Series Of Experiments ◽  
Rapd Makers ◽  
Grape Cultivars

The work reported here is an extension of studies reported in 1990. The general objective was to develop molecular markers for genotype `fingerprinting', with specific reference to possible clonal differences among `Pinot noir' clones. Leaf DNA from 8 cultivars and 9 `Pinot noir' clones were isolated. RFLP and RAPD markers were identified and used to characterize the genotypes. 65 32-P labelled cloned probes were constructed with the pUC18 plasmid and Hind-III digested `Pinot noir' DNA. The probes were tested for their ability to discriminate among the 8 cultivars. 3 probes pGAD10, pGAD15, and pGAD44 showed polymorphisms among the cultivars. pGAD15 was most useful, with 5 polymorphisms for the 8 cultivars. RAPD makers were also tested for `fingerprinting'. Several primers were tested and polymorphisms were identified among cultivars. However, significant problems with repeatability for some bands were observed. Therefore, a series of experiments were conducted to test the effect of season and extraction method. These factors did not account for the inconsistancy which seemed to be more a function of the primer used. None of these studies showed clear evidence that the `Pinot noir' clones tested were geetically different.

scholarly journals Prospects for Fingerprinting the USDA–ARS Cold-hardy Vitis Germplasm Collection using Simple Sequence Repeat (SSR) Markers

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 620e-620
Author(s):  
Warren F. Lamboy ◽  
Christopher G. Alpha
Keyword(s):  
Simple Sequence Repeat ◽  
Genetic Relationships ◽  
Plant Genetic Resources ◽  
Germplasm Collection ◽  
Genetic Identity ◽  
Dna Fragments ◽  
Sequence Repeat ◽  
Cold Hardy ◽  
Types Of Information ◽  
Simple Sequence

Curators of plant genetic resources collections must preserve germplasm possessing known useful characteristics as well as material displaying general genetic diversity. In order to ensure that both types of germplasm are included in a collection, germplasm curators require three fundamental types of information about each accession: taxonomic identity, genetic identity, and genetic relationship. Because simple sequence repeat DNA fragments (SSRs) have been successfully used to determine the genetic identity of grape clones, we conducted a study to determine if SSRs would supply all three types of information for the accessions in the cold-hardy Vitis (grape) germplasm collection. SSR fragments were amplified at six different loci for 23 accessions of cold-hardy grape spanning the range of species diversity in the collection. The minimum number of different alleles found at a locus was 9; the maximum was 26. Heterozygosity values ranged between 0.565 and 0.783, while gene diversity values were in the range 0.785 to 0.944. Two hundred fifty-two pairs of plants out of a possible 253 could be distinguished by their SSR profiles. Nei's genetic identities were computed between all pairs of plants and used in a UPGMA cluster analysis. The relationships obtained did not correspond well to expected relationships based on geography and taxonomy. Four species of grapes were represented by two or more accessions in this study. No DNA fragments found at these six loci served to unambiguously distinguish one species from another. Thus, SSR fragments from the six loci studied were useful in determining genetic identity of accessions, but were not helpful in determining genetic relationships or taxonomic identities. We are searching for additional loci that are informative for these types of information. Meanwhile we highly recommend SSRs for determining genetic identity in germplasm resources collections.

scholarly journals THE INFLUENCE OF HAIL AND TREATMENTS WITH AMINO-ACIDS ON THE FERTILITY AND BIOLOGIC BALANCE OF GRAPEVINES

2020 ◽  
Vol 1 (1) ◽  
pp. 111-118
Author(s):  
Sabin Baniță ◽  
Sabin Baniță ◽  
Adriana Sestraș ◽  
Ion Olteanu ◽  
Anamaria Călugăr ◽  
...  
Keyword(s):  
Amino Acids ◽  
Red Wine ◽  
White Wine ◽  
Climatic Changes ◽  
Pinot Noir ◽  
Natural Phenomenon ◽  
Vitis Vinifera L ◽  
Recovery Index ◽  
Grape Cultivars ◽  
Number Of Shoots

Climatic accidents increase in frequency due to climatic changes. Hailstorms are typically localized events and are known few studies about their effect on grapevine. The objective of this study was to examine the vine vegetative balance responses to natural hail, registered when shoots were 10-15 cm height. Four grape cultivars (Vitis vinifera L.), two for red wine (Feteascǎ Neagrǎ, Pinot Noir) and two for white wine (Chardonnay, Tǎmȃioasǎ romȃneascǎ) were studied in Dealu Mare, one of the most known vineyards in Romania. Visibly, the extent of the hailstorm damage was great, enough to injure or remove about 90% of shoots growth till hail fall. Three treatments with Delfan Plus (amino-acids complex) were applied on grapevine canopy. Number of shoots/vine and number of fertile shoots/vine were counted and biologic balance of grapevine indexes were calculated. Our results indicated that natural hail affect significantly the number of fertile shoots. Delfan Plus treatments positively influenced the number of shoots and fertile shoots. Delfan Plus treatments had little influence on Recovery index, vine Balance indexes and Ensurance Coefficient after one single year of study. The capacity of recovery after hail fall, in this case, is more influenced by the grape cultivar. The present work is the first attempt to enhance our understanding on the vegetative responses of grapevines to the use of biostimulants and to natural hail, which is an extreme and complex natural phenomenon.

scholarly journals Variation in Bloom Time in a Sour Cherry Germplasm Collection

HortScience ◽  
1992 ◽  
Vol 27 (10) ◽  
pp. 1113-1114 ◽  
Author(s):  
Amy F. Iezzoni ◽  
Colleen A. Mulinix
Keyword(s):  
United States ◽  
Interspecific Hybrid ◽  
Selection Pressure ◽  
Germplasm Collection ◽  
Sour Cherry ◽  
The United States ◽  
Prunus Cerasus ◽  
Commercially Important ◽  
Cold Hardy ◽  
Selection Of

Bloom times were evaluated for seedlings from four full-sib and 14 open-pollinated families of sour cherry (Prunus cerasus L.). Time of anthesis for individual seedlings ranged over 17and 16-day periods in 1989 and 1990, respectively. In both years, most seedlings bloomed later than `Montmorency', the only commercially important sour cherry cultivar in the United States. `Pitic de Iasi', the parent of the latest-blooming family, is a natural interspecific hybrid between sour cherry and the cold-hardy Russian ground cherry (P. fruticosa Pall.). Hybridization between sour and ground cherry and intense selection pressure in the colder areas of the sour cherry habitat may have favored selection of the late-blooming character.

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