scholarly journals MOLECULAR MARKERS FOR CHARACTERIZATION OF GRAPE CULTIVARS II

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 660f-660
Author(s):  
Y. Gogorcena ◽  
S. Arulsekar ◽  
D.E. Parfitt
Keyword(s):  
Molecular Markers ◽  
Extraction Method ◽  
Rapd Markers ◽  
Pinot Noir ◽  
Specific Reference ◽  
Series Of Experiments ◽  
Rapd Makers ◽  
Grape Cultivars

The work reported here is an extension of studies reported in 1990. The general objective was to develop molecular markers for genotype `fingerprinting', with specific reference to possible clonal differences among `Pinot noir' clones. Leaf DNA from 8 cultivars and 9 `Pinot noir' clones were isolated. RFLP and RAPD markers were identified and used to characterize the genotypes. 65 32-P labelled cloned probes were constructed with the pUC18 plasmid and Hind-III digested `Pinot noir' DNA. The probes were tested for their ability to discriminate among the 8 cultivars. 3 probes pGAD10, pGAD15, and pGAD44 showed polymorphisms among the cultivars. pGAD15 was most useful, with 5 polymorphisms for the 8 cultivars. RAPD makers were also tested for `fingerprinting'. Several primers were tested and polymorphisms were identified among cultivars. However, significant problems with repeatability for some bands were observed. Therefore, a series of experiments were conducted to test the effect of season and extraction method. These factors did not account for the inconsistancy which seemed to be more a function of the primer used. None of these studies showed clear evidence that the `Pinot noir' clones tested were geetically different.

scholarly journals Morphologic and molecular characterization of Myrciaria spp species

2005 ◽  
Vol 27 (3) ◽  
pp. 507-510 ◽  
Author(s):  
Márcio Pereira ◽  
Antonio Luis de Oliveira ◽  
Regina Eli de Almeida Pereira ◽  
Janete Apparecida Desidério Sena ◽  
Juliana Regina Vieira da Costa ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Rapd Markers ◽  
Fruit Trees ◽  
Sao Paulo ◽  
São Paulo ◽  
Paulo State ◽  
Morphologic Characteristics ◽  
Botanical Classification ◽  
Made In

The jabuticaba tree is considered one of the most typical Brazilian fruit trees. However, few studies of this plant are found in the literature and even its botanical classification is very controversial. The present research reports some comparisons between jabuticaba species, using morphologic (organography) and molecular markers techniques. The morphologic characteristics of the plant used as markers were compared with specimens of the herbaria from São Paulo and Minas Gerais States and with the descriptions reported in the literature. Molecular differences between the species were identified by the use of RAPD markers. The experiment was made in Piracicaba, Jaboticabal and Ituverava municipal districts in São Paulo State, Brazil. Morphologic and molecular differences between the studied plants were identified and four groups of species were defined: Myrciaria cauliflora (Mart.) O. Berg, M. coronata Mattos, M. jaboticaba (Vell.) O. Berg. and Myrciaria phytrantha (Kiaersk.) Mattos. Both molecular and morphologic markers techniques showed to be important tools for the identification of jabuticaba tree species.

scholarly journals Characterization of potato genotypes using molecular markers

2004 ◽  
Vol 39 (9) ◽  
pp. 871-878 ◽  
Author(s):  
Eliana Antonia Silveira Collares ◽  
Eva Choer ◽  
Arione da Silva Pereira
Keyword(s):  
Molecular Markers ◽  
Gel Electrophoresis ◽  
Rapd Markers ◽  
Polyacrylamide Gel Electrophoresis ◽  
Group Method ◽  
Jaccard Coefficient ◽  
Leaf Extracts ◽  
Arithmetic Average ◽  
Pair Group

The objective of this work was to characterize 27 potato genotypes, using molecular markers. Polyacrylamide gel electrophoresis, RAPD techniques and isozymes of esterase, phosphoglucomutase and soluble proteins were analyzed in tubers, and isocitrate dehydrogenase, aspartate transaminase, phosphoglucomutase and peroxidase, in leaves. Eighteen primers were tested and four were chosen, kits OPX (01, 04 and 09) and OPY (07), to analyze RAPD markers in leaf extracts. Similarity and cluster analysis were conducted using Jaccard coefficient and the unweighted pair-group method using arithmetic average. Despite the differences detected in the analysis of proteins and isozymes in the tubers, as well as of isozymes in the leaves, the characterization of all genotypes through gel electrophoresis was not possible, while RAPD markers were efficient to characterize all the 27 genotypes.

scholarly journals Morphological and genetic characterization of vine grape cultivars of Herzegovina

2017 ◽  
Vol 3 (2) ◽  
pp. 1-9
Author(s):  
Zrinka Knezović ◽  
Ana Mandić ◽  
Nikica Perić ◽  
Jure Beljo ◽  
Maja Žulj Mihaljević
Keyword(s):  
Molecular Markers ◽  
Genetic Characterization ◽  
Morphological Characterization ◽  
Similarity Coefficient ◽  
Value Range ◽  
Grape Cultivars

Abstract In Herzegovina, different genotypes of grapevine are called the same, and the origin of these cultivars is mainly unexplored. Two groups of autochthonous cultivars are grown. The first group consists of more widespread Žilavka and Blatina, and accompanying Bena, Krkošija, Dobrogostina and Trnjak, and the second one of less common cultivars that should be preserved from extinction. Considering the small number of studies on the comparison of different methods used for analyses of similarities and relatedness of grapevine germplasm, it was interesting to assess the use of morphological characterization with molecular markers in identification of cultivars in Herzegovina. The results indicate that a similarity coefficient had a broad value range for both methods. Both methods successfully in different ways differentiated all analysed varieties, and for complete and comprehensive identification of grapevine varieties should be use.

scholarly journals Characterization of sweet cassava accessions based on molecular, quantitative and qualitative data

2011 ◽  
Vol 11 (3) ◽  
pp. 232-240 ◽  
Author(s):  
Eduardo Alano Vieira ◽  
Josefino de Freitas Fialho ◽  
Fábio Gelape Faleiro ◽  
Graciele Bellon ◽  
Kenia Graciele da Fonseca ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Quantitative Traits ◽  
Rapd Markers ◽  
Qualitative Data ◽  
Germplasm Bank ◽  
Qualitative And Quantitative ◽  
Qualitative Characters ◽  
Genetic Dissimilarity ◽  
Sweet Cassava

The purpose of this study was to estimate the genetic divergence in sweet cassava accessions by molecular markers and quantitative and qualitative characters, as well as determine the correlation between these estimates. Sixteen sweet cassava accessions of the Regional Cassava Germplasm Bank of the Cerrado were evaluated under field conditions, for 13 quantitative and 33 qualitative characters. In the laboratory, the accessions were evaluated with RAPD markers. Subsequently, matrixes of genetic dissimilarity/distance among the accessions were estimated based on molecular markers and quantitative and qualitative characters. Besides, the significance of the correlation between the matrixes was estimated. The RAPD, qualitative and quantitative data indicated the existence of high divergence among the accessions. The divergences estimated by molecular markers and by quantitative traits were weakly associated with each other and moderately with the divergence estimated by qualitative characters.

Evaluation of Molecular Markers Specific for 1BL∙1RS Translocation and Characterization of 1RS Chromosome in Wheat Varieties from Different Origins

2009 ◽  
Vol 35 (11) ◽  
pp. 2107-2115 ◽  
Author(s):  
Huai-Jun TANG ◽  
Gui-Hong YIN ◽  
Xian-Chun XIA ◽  
Jian-Jun FENG ◽  
Yan-Ying QU ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Wheat Varieties ◽  
Different Origins

Genetic Diversity of Basil (Ocimum spp. - Lamiaceae) Based on RAPD Markers and Volatile Oil Constituents

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 492f-493
Author(s):  
Roberto F. Vieira ◽  
James E. Simon ◽  
Peter Goldsbrough ◽  
Antonio Figueira
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Volatile Oil ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Traditional Medicines ◽  
The Tropics ◽  
High Degree ◽  
First Time

Essential oils extracted from basil (Ocimum spp.) by steam distillation are used to flavor foods, oral products, in fragrances, and in traditional medicines. The genus Ocimum contains around 30 species native to the tropics and subtropics, with some species naturalized and/or cultivated in temperate areas. Interand intraspecific hybridization have created significant confusion in the botanical systematics of this genus. Taxonomy of basil (O. basilicum) is also complicated by the existence of numerous varieties, cultivars, and chemotypes within the species that do not differ significantly in morphology. In this study we are using RAPD markers and volatile oil composition to characterize the genetic diversity among the most economically important Ocimum species. We hypothesize that the genetic similarity revealed by molecular markers will more accurately reflect the morphological and chemical differences in Ocimum than essential oil composition per se. Preliminary research using five Ocimum species, four undetermined species, and eight varieties of O. basilicum (a total of 19 accessions) generated 107 polymorphic fragments amplified with 19 primers. RAPDs are able to discriminate between Ocimum species, but show a high degree of similarity between O. basilicum varieties. The genetic distance between nine species and among 55 accessions within the species O. americanum, O. basilicum, O. campechianum, O. × citriodorum, O. gratissimum, O. kilimandscharium, O. minimum, O. selloi, and O. tenuiflorum will be analyzed by matrix of similarity and compared to the volatile oil profile. This research will for the first time apply molecular markers to characterize the genetic diversity of Ocimum associate with volatile oil constituent.

Characterization of High-Copy-Number Retrotransposons From the Large Genomes of the Louisiana Iris Species and Their Use as Molecular Markers

Genetics ◽  
2003 ◽  
Vol 164 (2) ◽  
pp. 685-697 ◽  
Author(s):  
Edward K Kentner ◽  
Michael L Arnold ◽  
Susan R Wessler
Keyword(s):  
Molecular Markers ◽  
Copy Number ◽  
High Copy Number ◽  
Marker System ◽  
Transposon Display ◽  
Plant Genomes ◽  
Large Plant ◽  
Backcross Hybrids ◽  
Abundance And Distribution

Abstract The Louisiana iris species Iris brevicaulis and I. fulva are morphologically and karyotypically distinct yet frequently hybridize in nature. A group of high-copy-number TY3/gypsy-like retrotransposons was characterized from these species and used to develop molecular markers that take advantage of the abundance and distribution of these elements in the large iris genome. The copy number of these IRRE elements (for iris retroelement), is ∼1 × 105, accounting for ∼6–10% of the ∼10,000-Mb haploid Louisiana iris genome. IRRE elements are transcriptionally active in I. brevicaulis and I. fulva and their F1 and backcross hybrids. The LTRs of the elements are more variable than the coding domains and can be used to define several distinct IRRE subfamilies. Transposon display or S-SAP markers specific to two of these subfamilies have been developed and are highly polymorphic among wild-collected individuals of each species. As IRRE elements are present in each of 11 iris species tested, the marker system has the potential to provide valuable comparative data on the dynamics of retrotransposition in large plant genomes.

Cloning and characterization of RAPD markers of parasitic nematodesTrichinella spiralis andT. pseudospiralis

2000 ◽  
Vol 34 (5) ◽  
pp. 702-706
Author(s):  
G. G. Chrisanfova ◽  
S. K. Semyenova ◽  
A. P. Ryskov
Keyword(s):  
Rapd Markers
Sign in / Sign up

Export Citation Format

Share Document