scholarly journals Responses of Broccoli Seedlings to Light Quality during Low-temperature Storage in Vitro: II. Sugar Content and Photosynthetic Efficiency

HortScience ◽  
1998 ◽  
Vol 33 (7) ◽  
pp. 1258-1261 ◽  
Author(s):  
Sandra B. Wilson ◽  
Keiko Iwabuchi ◽  
Nihal C. Rajapakse ◽  
Roy E. Young
Keyword(s):  
Low Temperature ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Photon Flux ◽  
Media Composition ◽  
Low Temperature Storage ◽  
Photosynthetic Ability ◽  
Temperature Storage

Broccoli (Brassica oleracea L. Botrytis group `Green Duke') seeds were cultured photoautotrophically (without sugar) or photomixotrophically (with sugar) in vitro for 3 weeks at 23 °C and150 μmol·m-2·s-1 photosynthetic photon flux (PPF). In vitro seedlings were stored for 0, 4, 8, or 12 weeks at 5 °C in darkness or under 5 μmol·m-2·s-1 of white (400–800 nm), blue (400–500 nm), or red (600–700 nm) light. Photosynthetic ability and soluble sugar contents were determined after removal from storage. Photomixotrophic seedlings contained approximately five times more soluble sugars than did photoautotrophic seedlings. Dark storage reduced soluble sugars in both photoautotrophic and photomixotrophic plants, but photosynthetic ability was maintained for up to 8 weeks in the latter whereas it decreased in the former. Illumination in storage increased leaf soluble sgars in both photoautotrophic and photomixotrophic seedlings. Soluble sugars in stems decreased during storage regardless of illumination, but remained higher in illuminated seedlings. Red light was more effective in increasing or maintaining leaf and stem soluble sugars than was white or blue light. Regardless of media composition or illumination, storage for more tan 8 weeks resulted in dramatic losses in quality and recovery, as well as photosynthetic ability. Seedlings stored for 12 weeks comletely lost their photosynthetic ability regardless of media composition or illumination. The results suggest that carbohydrate, supplied in the media or through illumination, is essential for maintenance of photosynthetic ability during low-temperature storage for up to 4 or 8 weeks.

scholarly journals The Effect of Light and Temperature on the Physiological Status of Bedding Plant Plugs during Short-term Low-temperature Storage

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 517C-517
Author(s):  
Efstratia Papanikou ◽  
Paul H. Jennings
Keyword(s):  
Low Temperature ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Weather Conditions ◽  
Physiological Status ◽  
Growth Responses ◽  
Short Term ◽  
Low Temperature Storage ◽  
Bedding Plant ◽  
Temperature Storage

Previous research has shown that low-temperature storage can be used to maintain bedding plants in plug trays when weather conditions in spring make scheduling of transplanting difficult. The objective of this study was to determine what physiological changes occur during the short-term, low-temperature storage of plug seedlings. Plants of two bedding plant species, Geranium and Vinca, were stored at 2, 6, or 10°C and under low light or dark conditions for 4 weeks. Data were collected at three sampling dates (0, 2, or 4 weeks after beginning of storage) and included dry and fresh mass, total leaf area, leaf chlorophyll content and chlorophyll fluorescence as well as electrolyte leakage and soluble sugar content of leaf and root tissue. The parameters will be discussed in relationship to plug seedling survivability, quality, and growth responses under the experimental storage treatments.

scholarly journals Low-temperature Storage of Rooted Chrysanthemum Cuttings: Relationship to Carbohydrate Status of Cultivars

1996 ◽  
Vol 121 (4) ◽  
pp. 740-745 ◽  
Author(s):  
Nihal C. Rajapakse ◽  
William B. Miller ◽  
John W. Kelly
Keyword(s):  
Low Temperature ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Storage Duration ◽  
Rooted Cuttings ◽  
Low Temperature Storage ◽  
Leaf Necrosis ◽  
Storage Potential ◽  
Temperature Storage

Low-temperature storage potential of rooted cuttings of garden chrysanthemum [Dendranthema ×grandiflorum (Ramat.) Kitamura] cultivars and its relationship with carbohydrate reserves were evaluated. Storage of chrysanthemum cuttings at -1 and -3 °C resulted in freezing damage. Visual quality of rooted cuttings stored at 0 or 3 °C varied among cultivars. Quality of `Emily' and `Naomi' cuttings was reduced within a week by dark storage at 0 or 3 °C due to leaf necrosis, while `Anna' and `Debonair' cuttings could be held for 4 to 6 weeks without significant quality loss. In `Anna' and `Debonair', low-temperature storage reduced the number of days from planting to anthesis regardless of storage duration. However, flowers of plants grown from stored cuttings were smaller than those of nonstored cuttings. At the beginning of storage, `Emily' and `Naomi' had lower sucrose, glucose, and fructose (soluble sugars) content compared to `Anna' and `Debonair'. Regardless of temperature, leaf soluble sugar was significantly reduced by dark storage for 4 weeks. In stems, sucrose and glucose were reduced while fructose generally increased during low-temperature storage probably due to the breakdown of fructans. Depletion of soluble sugars and a fructan-containing substance during low-temperature dark storage was greater in `Emily' and `Naomi' than in `Anna' and `Debonair'. Low irradiance [about 10 μmol·m-2·s-1 photosynthetically active radiation (PAR) from cool-white fluorescent lamps] in storage greatly improved overall quality and delayed the development of leaf necrosis in `Naomi'. Cuttings stored under light were darker green and had a higher chlorophyll content. Leaf and stem dry weights increased in plants stored under medium and high (25 to 35 μmol·m-2·s-1 PAR) irradiance while no change in dry weight was observed under dark or low light. Results suggest that the low-temperature storage potential of chrysanthemum cultivars varies considerably, and provision of light is beneficial in delaying the development of leaf necrosis and maintaining quality of cultivars with short storage life at low temperatures.

scholarly journals Low-temperature Storage for Quality Preservation and Growth Suppression of Broccoli Plantlets Cultured in Vitro

HortScience ◽  
1994 ◽  
Vol 29 (10) ◽  
pp. 1191-1194 ◽  
Author(s):  
Chieri Kubota ◽  
Toyoki Kozai
Keyword(s):  
Low Temperature ◽  
Dry Weight ◽  
Photon Flux ◽  
Photosynthetic Photon Flux ◽  
Fluorescence Kinetics ◽  
Low Temperature Storage ◽  
Fluorescent Lamps ◽  
Chlorophyll Concentrations ◽  
Temperature Storage

Broccoli (Brassica oleracea L. Botrytis Group `Ryokurei') plantlets, cultured photoautotrophically (without sugar in the medium) in vitro for 3 weeks at 23C and 160 μmol·m–2·s–1 photosynthetic photon flux (PPF), were stored for 6 weeks at 5, 10, or 15C under 0 (darkness) or 2 μmol·m–2·s–1 PPF (continuous lighting) supplied by fluorescent lamps (white light). Dry weight of the plantlets stored for 6 weeks at 5 or 10C in light was not significantly different from that of the plantlets before storage. Dry weight of the plantlets decreased as temperature increased and was maintained at higher levels in light than in darkness. Chlorophyll concentrations of the plantlets were higher at the lower temperatures. Chlorophyll fluorescence kinetics indicated higher activities of chlorophyll of the plantlets stored in light than in darkness. Lighting at as low as 2 μmol·m–2·s–1 PPF was important to preserve photosynthetic and regrowth abilities and dry weight of the plantlets during low-temperature storage.

scholarly journals Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollen

2006 ◽  
Vol 108 (1) ◽  
pp. 91-94 ◽  
Author(s):  
J. Lora ◽  
M.A. Pérez de Oteyza ◽  
P. Fuentetaja ◽  
J.I. Hormaza
Keyword(s):  
Low Temperature ◽  
In Vitro Germination ◽  
Low Temperature Storage ◽  
Annona Cherimola ◽  
Temperature Storage

scholarly journals Hypothermic and Low-Temperature Storage of Garlic (Allium sativum L.) for in Vitro Collections

2017 ◽  
Vol 27 (2) ◽  
pp. 110-120
Author(s):  
Tetyana V. Ivchenko ◽  
◽  
Tatyana I. Vitsenya ◽  
Nadiya A. Shevchenko ◽  
Natalia O. Bashtan ◽  
...  
Keyword(s):  
Low Temperature ◽  
Allium Sativum ◽  
Low Temperature Storage ◽  
Temperature Storage

scholarly journals Optimum Harvest of Low-chill Melting and Non-melting Flesh Peach Cultivars for Direct Ripening and Ripening following Low Temperature Storage

HortScience ◽  
2020 ◽  
Vol 55 (4) ◽  
pp. 487-495
Author(s):  
Ming-Wei S. Kao ◽  
Jeffrey K. Brecht ◽  
Jeffrey G. Williamson
Keyword(s):  
Low Temperature ◽  
Linear Correlation ◽  
Soluble Sugar ◽  
Storage Conditions ◽  
Soluble Solids ◽  
Total Soluble Sugar ◽  
Low Temperature Storage ◽  
Flesh Firmness ◽  
Harvest Maturity ◽  
Temperature Storage

The physical and chemical characteristics of two melting flesh (MF) cultivars, TropicBeauty and Flordaprince, and two non-melting flesh (NMF) cultivars, UFSun and Gulfking, with advancing maturities, were determined at harvest, after ripening at 20 °C for 7 days (i.e., direct ripening) and after storage at 0 °C for 14 days then ripening at 20 °C for 7 days (i.e., ripening following low temperature storage). The NMF cultivars were able to retain flesh firmness better than the MF cultivars as fruit matured and ripened on the tree and after the two storage treatments. The NMF fruit of the least mature to the most advanced maturity groups (MGs) were ≈2 to 7 times firmer than the MF fruit in the same MGs after ripening in both storage conditions. For both MF and NMF fruit, a significant reduction of titratable acidity (TA) occurred with no significant changes in soluble solids content (SSC) and total soluble sugar (TSS) as maturity and ripening progressed on the tree and after ripening in both storage conditions. Minimum quality standards of “ready for consumption” peaches were used as general guidelines to determine the optimum harvest maturity of all four cultivars. The NMF fruit ripened directly had wider optimum harvest maturity ranges and could be harvested at more advanced stages than the MF fruit. The MF fruit that ripened following low temperature storage needed to be picked at earlier maturity stages than those that were directly ripened. The optimum harvest maturity of NMF UFSun for the low temperature storage treatment was more advanced than that of the other three cultivars due to abnormal softening found in the lower MGs after ripening. Linear correlation analyses showed that the skin ground color (GC) a* values of both MF cultivars and NMF ‘UFSun’ were highly correlated with the flesh color (FC) a* values, suggesting that GC a* values can be an informative harvest indicator for this NMF cultivar instead of the traditionally used FC. The GC a* values also had high linear correlation with TA for all four cultivars, suggesting that TA can be a potential maturity index for both MF and NMF peaches. Significant correlations of GC a* values and flesh firmness (GC-FF) were found in all four cultivars in one year but only in MF peaches in both years, showing that flesh firmness was the most consistent maturity indicator for the MF cultivars in this study.

scholarly journals Proteomic and metabolic profile analysis of low-temperature storage responses in Ipomoea batata Lam. tuberous roots

2020 ◽  
Author(s):  
Peng Cui ◽  
Yongxin Li ◽  
Chenke Cui ◽  
Yanrong Huo ◽  
Guoquan Lu ◽  
...  
Keyword(s):  
Low Temperature ◽  
Metabolic Pathways ◽  
Metabolic Profile ◽  
Soluble Sugar ◽  
Sucrose Metabolism ◽  
Glucosinolate Biosynthesis ◽  
Low Temperature Storage ◽  
Leading Role ◽  
Tuberous Roots ◽  
Temperature Storage

Abstract Background: Sweetpotato (Ipomoea batatas L.) is one of the seven major food crops grown worldwide, with 70-75% of production in China. Cold stress often can cause protein expression pattern and substance contents variations for tuberous roots of sweetpotato during low-temperature storage. Recently, we developed proteometabolic profiles of the fresh sweetpotatoes (cv. Xinxiang) in an attempt to discern the cold stress-responsive mechanism of tuberous root crops during post-harvest storage. Results: For roots stored under 4℃ condition, the CI index, REC and MDA content in roots were significantly higher than them at control temperature (13℃). The activities of SOD, CAT, APX, O2.- producing rate, proline and especially soluble sugar contents were also significantly increased. Most of the differentially expressed proteins (DEPs) were implicated in pathways related to metabolic pathway (~22%), especially phenylpropanoids (~10%) and followed by starch and sucrose metabolism (~3%). Proteins including L-ascorbate peroxidase 3 and catalase were down-regulated during low temperature (4℃) storage. α-amylase, sucrose synthase and fructokinase were significantly up-regulated in starch and sucrose metabolism, while β-glucosidase, glucose-1-phosphate adenylyl-transferase and starch synthase were opposite. Furthermore, metabolome profiling revealed that glucosinolate biosynthesis, tropane, piperidine and pyridine alkaloid biosynthesis as well as protein digestion and absorption played a leading role in metabolic pathways of sweetpotato roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis. Data are available via ProteomeXchange with identifier PXD017728.Conclusions: Our proteomic and metabolic profile analysis of sweetpotato roots stored at low temperature reveal that the antioxidant enzymes activities, proline and especially soluble sugar content were significantly increased. Most of the DEPs were implicated in phenylpropanoids and followed by starch and sucrose metabolism. The discrepancy between proteomic (L-ascorbate peroxidase 3 and catalase) and biochemical (APX/CAT enzyme activity) data may be explained by higher H2O2 levels and increased glutathione and ascorbate redox states, which enhanced the CAT/APX enzyme activity indirectly. Glucosinolate biosynthesis played a leading role in metabolic pathways of sweetpotao roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis.

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