scholarly journals 186 Berry Quality and Photosynthate Partitioning in Response to Plant Growth Regulators in Grape

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 422E-422
Author(s):  
H. Yakushiji ◽  
K. Morinaga ◽  
Y. Koshita
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Soluble Solids ◽  
Naphthaleneacetic Acid ◽  
Anthocyanin Content ◽  
Grape Berries ◽  
Berry Quality ◽  
C Distribution ◽  
Berry Maturation

The effects of 2,3,5-triiodobenzoic acid (TIBA) and naphthaleneacetic acid (NAA) on berry maturation and photoassimilates partitioning were investigated. Five-year-old potted `Kyoho' grape grown under a non-heating glasshouse were used. TIBA (200 mg/L) and NAA (200 mg/L) were applied to clusters at the beginning of veraison (45 days after full bloom). TIBA application increased not only soluble solids concentration in the juice but also anthocyanin content of peel, compared with those of control. On the other hand, the application of NAA reduced berry growth and delayed the berry maturation with harder flesh, lower soluble solids, higher acidity and poor coloration. In order to examine the effect of both plant growth regulators on photoassimilates partitioning in plant tissues, the whole plants were fed with 13CO2 at 10 days and 20 days after application of TIBA and NAA. The 13C distribution of pericarp and peel in NAA application was found on the lowest among the treatments. However, there were no significant differences in the 13C distribution and 13C absorption rate of pericarps between TIBA and control. These results indicate that NAA weakened the sink activity in grape berries, resulted in smaller berry size and the delay of maturation, whereas the berry ripening induced by TIBA application could not be explained by the distribution of photoassimilates in grape berries.

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

scholarly journals In vitro organogenesis and plant regeneration of Passiflora xishuangbannaensis, a species with extremely small populations

2021 ◽  
Author(s):  
Yuan-yuan Meng ◽  
Shi-jie Song ◽  
Sven Landrein
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Basal Medium ◽  
Ex Situ ◽  
Naphthaleneacetic Acid ◽  
South American ◽  
South American Species

Abstract Passiflora xishuangbannaensis (Passifloraceae) is endemic to a few sites of Mengyang nature reserve in Yunnan, Xishuangbanna and less than 40 individuals have been recorded. Nine Passiflora species are endemic to Yunnan with most species occurring in South America, making P. xishuangbannaensis highly significant and emblematic to the conservation work in the region. This study is designed to provide the first protocol for in vitro organogenesis and plant regeneration for ex situ conservation and reintroduction for an Asian Passiflora species. Using internodes, petioles and tendrils we optimize calli formation and root elongation using several plant growth regulators, individually or in combination. We also assess the genetic stability of regenerated cells. The maximum callus induction and shoot bud differentiation were both achieved on half Murashige and Skoog basal medium supplemented with 4.44 µM 6-Benzylaminopurine and 1.08 µM 1-Naphthaleneacetic acid. The best rooting was achieved from 30 days old, regenerated shoots on half Murashige and Skoog basal medium supplemented with 1.08 µM 1-Naphthaleneacetic acid. Micropropagated plants were subjected to inter simple sequence repeat markers analyses. Collectively, 86 bands were generated from 6 primers of which 12 bands were polymorphic, showing genetic variation between the regenerated plantlets and the original plant. Response to plant growth regulators was more specific than most other studies using South American species, which could be explained by the morphological and physiological differences between South American and Asian Passiflora species

scholarly journals In vitro Multiplication of the Pitcher Plant Sarracenia Purpurea

2018 ◽  
Vol 75 (2) ◽  
pp. 134
Author(s):  
Ileana MICLEA ◽  
Rita BERNAT
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Multiplication ◽  
Time Frame ◽  
Naphthaleneacetic Acid ◽  
Sarracenia Purpurea ◽  
Pitcher Plant ◽  
Frame Number

The aim of the current research was to find the best plant growth regulators for the multiplication of Sarracenia purpurea. Murashige and Skoog medium (MS) was prepared with macronutrients and micronutrients at 1/3 strength, full strength vitamins, supplemented with 30 g/l sucrose and 5 g/l phytagel and autoclaved. After cooling 0.5 mg\l α-naphthaleneacetic acid (NAA), 5 mg\l 6-benzyladenine (BA) or 0.5 mg\l NAA + 3 mg\l BA were added. Young S. purpurea plants were selected and transferred to media with or without plant growth regulators and cultured for 12 weeks. At the end of this time frame number of roots, root length (cm) and number of shoots were evaluated and differences were analysed by the analysis of variance and interpreted using the Tuckey test. The largest number of roots grew in medium supplemented with 0.5 mg\l NAA but the the absence of plant growth regulators increased their length. The best conditions for shoot multiplication were provided by supplementing 1/3MS with 5 mg\l BA.

scholarly journals Plant growth regulators to enhance fruit color of 'Gala' apples

2017 ◽  
Vol 52 (11) ◽  
pp. 1118-1122 ◽  
Author(s):  
Alberto Fontanella Brighenti ◽  
Douglas André Würz ◽  
Mateus da Silveira Pasa ◽  
Leo Rufato
Keyword(s):  
Abscisic Acid ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Positive Control ◽  
Fruit Weight ◽  
Fruit Color ◽  
Soluble Solids ◽  
Flesh Firmness ◽  
Red Color

Abstract: The objective of this work was to investigate the effect of plant growth regulators for enhancing fruit color of 'Gala Standard' apples (Malus domestica). The experiment was carried out in the 2015 and 2016 crop seasons. The treatments consisted of water, as a control; 300 mg L-1 ethephon, as a positive control; 400 mg L-1 prohydrojasmonate; and 400 mg L-1 abscisic acid. Flesh firmness, soluble solids content, fruit weight, and red color were assessed after harvest. Plant growth regulators enhanced red color of fruit and chlorophyll degradation. Prohydrojasmonate and abscisic acid did not reduce flesh firmness, in the 2016 season.

Multiresidue Analysis of Plant Growth Regulators in Grapes by Triple Quadrupole and Quadrupole–Time of Flight-Based Liquid Chromatography/Mass Spectrometry

2011 ◽  
Vol 94 (6) ◽  
pp. 1715-1721 ◽  
Author(s):  
Dasharath P Oulkar ◽  
Kaushik Banerjee ◽  
Sunil Kulkarni
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Multiple Reaction Monitoring ◽  
Time Of Flight ◽  
Accurate Mass ◽  
Multiresidue Analysis ◽  
Chlormequat Chloride ◽  
Grape Berries

Abstract A selective and sensitive LC-MS/MS method is presented for simultaneous determination of 12 plant growth regulators, viz., indol-3-acetic acid, indol-3-butyric acid, kinetin, zeatin, 6-benzyl aminopurine, gibberellic acid, abscisic acid, chlormequat chloride, forchlorfenuron, paclobutrazole, daminozide, and 2,4-dichlorophenoxy acetic acid, in bud sprouts and grape berries. The sample preparation method involved extraction of homogenized sample (5 g) with 40 mL methanol (80%), and final determination was by LC-MS/MS in the multiple reaction monitoring (MRM) mode with time segmentation for quantification supported by complementary analysis by quadrupole-time of flight (Q-TOF) MS with targeted high-resolution MS/MS scanning for confirmatory identification based on accurate mass measurements. The recovery of the test compounds ranged within 90–107% with precision RSD less than 5% (n = 6). The method could be successfully applied in analyzing incurred residue samples, and the strength of accurate mass analysis could be utilized in identifying the compounds in cases where the qualifier MRM ions were absent or at an S/N less than 3:1 due to low concentrations.

TECHNOLOGICAL PROFILE OF SWEET SORGHUM JUICE cv. CMSXS-646 SUBMITTED TO CHEMICAL RIPENERS APPLICATION AND SAMPLING PERIODS

2018 ◽  
Vol 17 (3) ◽  
pp. 420 ◽  
Author(s):  
BRUNO RAFAEL ALMEIDA MOREIRA ◽  
RONALDO SILVA VIANA ◽  
GUSTAVO PAVAN MATEUS ◽  
MATHEUS LUIS OLIVEIRA CUNHA
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Sweet Sorghum ◽  
Reducing Sugars ◽  
Statistical Tests ◽  
Hydraulic Press ◽  
Soluble Solids ◽  
Control Treatment ◽  
Total Reducing Sugars

ABSTRACT - There is a little information about sweet sorghum crop behavior under chemical ripeners spraying management. Therefore, the aim of this study was to assess the technological profile of sweet sorghum (cv. CMSXS-646) juice under spraying of plant growth regulators and sampling periods. A randomized complete block experimental factorial design was used, with an additional control treatment (arrangement 2x2+1), corresponding to two chemical ripeners with three-doses for each product, glyphosate (0.2, 0.4, and 0.8 l c.p. ha-1) and methyl-sulfumeturon (10, 20, and 30 g c.p. ha-1), two sampling periods (15 and 30-days after spraying - DAS) and one control treatment (water spraying). After crop harvest, stalks were milled using a hydraulic press and the derived juice was assessed for total soluble solids (°Brix), apparent sucrose (POL), purity (PRT), reducing sugars (RS), total reducing sugars (TRS), fibers (FIB) and recoverable theoretical sugars (RTS). The dataset was subjected to the Shapiro-Wilk, Fisher, Tukey, Pearson and multiple linear regression statistical tests (p<0.05). There was an interaction between causes of variation for almost all assessed characteristics except to RTS, only affected by the sampling period. As conclusion, it is possible to grow sweet sorghum (cv. CMSXS-646) till thirty-days after spraying of chemical ripeners, treated by glyphosate 0.4 l c.p. ha-1 or sulfumeturon-methyl 10 g c.p. ha-1, because these doses caused feedstock with high °Brix, POL, total reducing sugars, purity levels.Keywords: Sorghum spp., phytorregulators, plant growth regulators, glyphosate, sulfumeturon-methyl. PERFIL TECNOLÓGICO DE CALDO DE SORGO SACARINO (cv. CMSXS-646) SUBMETIDO À APLICAÇÃO DE MATURADORES QUÍMICOS E ÉPOCAS DE AMOSTRAGEMRESUMO – Há poucas informações sobre o comportamento da cultura do sorgo sacarino sob manejo de aplicação de maturadores químicos. Portanto, o objetivo desta pesquisa foi avaliar o perfil tecnológico de caldo de sorgo sacarino cv. CMSXS-646 submetido à aplicação de maturadores químicos e períodos de amostragem. Aplicou-se o delineamento de blocos inteiramente casualizados, em esquema de fatorial (2x2+1), correspondente a dois maturadores químicos com três dosagens - glifosato (0,2; 0,4 e 0,8 l p.c. ha-1) e sulfumeturon-metil (10, 20 e 30 g p.c. ha-1); dois períodos de amostragem (15 e 30 dias após aplicação - DAA); e um tratamento controle (aplicação de água). Após a colheita, destinaram-se colmos à moagem, realizada em prensa hidráulica, para extração de caldo; avaliaram-se sólidos solúveis totais (°Brix), sacarose aparente (POL), pureza (PRT), açúcares redutores (AR), açúcares redutores totais (ART), fibras (FIB) e açúcares teóricos recuperáveis (ATR). Ao conjunto de dados, aplicaram-se testes estatísticos, Shapiro- Wilk, Fisher, Tukey, Pearson e regressão múltipla, ao nível de 5% de probabilidade. Houve interação entre as causas de variação para quase todas as características, exceto ATR, influenciado, somente, pelo período de amostragem. Concluiu-se que a aplicação dos maturadores químicos glifosato 0,4 l c.p. ha-1 e sulfumeturon-metil 10 g p.c. ha-1 apresentou um incremento na qualidade da matéria-prima com alto teor de °Brix, POL, açúcares redutores totais e níveis de pureza trinta dias após a pulverização dos maturadores.Palavras-chave: Sorghum spp., fitorreguladores, reguladores de crescimento vegetal,

scholarly journals Effect of plant growth regulators on two different types of eggplant flowers regarding style length and fruit setting

2021 ◽  
Vol 19 (4) ◽  
pp. e0906-e0906
Author(s):  
Sedighehsadat Khaleghi ◽  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Fruit Set ◽  
Floral Morphology ◽  
Pollen Viability ◽  
Polar Axis ◽  
Naphthaleneacetic Acid ◽  
Fruit Setting ◽  
Final Yield

Aim of study: (i) to explore differences between eggplant flowers capable of setting fruit including long (LGs) and medium style flowers (MEs) and those which suffer from severe problems with fertility and fruit setting including short style ones (SRTs); (ii) to study the effect of plant growth regulators on floral morphology and fruit setting. Area of study: Isfahan University of Technology, Isfahan, Iran, 2017 and 2018. Material and methods: First the floral morphology and initial fruit setting of 13 eggplant genotypes from Iran were investigated. Then the differences between LGs and SRTs of two genotypes were explored. Finally, the effect of 1-naphthaleneacetic acid (NAA) and spermidine (Spd) on floral morphology and initial and final fruit setting of these two genotypes was determined. Main results: Results showed SRTs were not capable of fruit setting. Compared to SRTs, LGs had larger central canals, higher protein, total sugar, reducing sugar and K concentrations, as well as longer polar axis and pollen tubes and greater pollen viability. Although 1.5 mM Spd and 20 mg L-1 NAA resulted in increasing of LGs and MEs, and also total initial fruit set, surprisingly, no significant differences were observed in the final yield and final fruit set between the control and these treatments. Researching highlights: Since the rate of fruit dropping was higher in those treatments compared to the control, plants with more SRTs likely regulate their final load by abscising their flowers, and plants with more LGs regulate them by abscising their fruits.

scholarly journals Investigation of callogenesis and indirect regeneration of Freesia × hybrida Bailey ‘Argenta’

2015 ◽  
Vol 27 (2) ◽  
pp. 123-134 ◽  
Author(s):  
Ali Pourkhaloee ◽  
Morteza Khosh-Khui
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Cold Storage ◽  
Embryogenic Callus ◽  
Naphthaleneacetic Acid ◽  
Light Conditions ◽  
High Quality ◽  
Freesia Hybrida ◽  
Different Types

AbstractAn investigation was conducted to study the effects of explant sources, plant growth regulators, carbohydrates and light conditions on indirect cormlet regeneration and the induction of embryogenic callus of freesia (Freesia×hybridaBailey ‘Argenta’). Sections of two different types of explants, corms and pupae (cold storage-produced corms), were placed on Murashige and Skoog (MS) media containing different concentrations of plant growth regulators. The results showed that the highest percentage of callus induction (100%), the highest callus growth (15 mm diameter) and the best type of calli were achieved for pupa explants grown on the medium that contained 4 mg L−11-naphthaleneacetic acid (NAA) and 2 mg L−16-benzylaminopurine (BAP) in the dark. Increasing BAP up to 3 to 4.5 mg L−1resulted in the maximum number of regenerated cormlets from 1 cm2calli (2 cormlets) under light conditions. Overall, the best rooting of regenerated cormlets was achieved on MS media supplemented with 1 mg L−1indole-3-butyric acid (IBA). In the next stage, high quality calli were subcultured on MS media containing sorbitol, sucrose, maltose and mannitol (0, 5, 10 and 15 g L−1). The results indicated that 15 g L−1maltose was able to induce the highest percentage of embryogenic callus, with an average of 88.9% on media containing 2 mg L−1BAP and 1 mg L−1NAA.

Exogenous application of plant growth regulators enhances color and anthocyanin content of cherry fruits

2017 ◽  
pp. 621-626 ◽  
Author(s):  
E.G.L. Nagpala ◽  
M. Noferini ◽  
L. Piccinini ◽  
G. Fiori ◽  
S. Vidoni ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Anthocyanin Content ◽  
Exogenous Application

scholarly journals Direct and Efficient Plant Regeneration from Different Explants Sources of Potato Cultivars as Influenced by Plant Growth Regulators

2012 ◽  
Vol 12 ◽  
pp. 1-6 ◽  
Author(s):  
Shambhu P. Dhital ◽  
Hak T. Lim ◽  
Hira K. Manandhar
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Solid Medium ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Petiole Explants ◽  
Number Of Shoots

Response of widely grown potato cv. Superior and newly developed cvs. Gui valley and Bora valley to plant growth regulators (PGRs) for direct plant regeneration from internode, leaf blade and petiole explants were investigated. The explants were cultured on a MS solid medium supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA), zeatin, indole-3-acetic acid (IAA) and gibberellic acid (GA3). Potato cv. Superior, regenerated direct shoot without callus and root formation on MS solid medium supplemented with BAP or zeatin, proliferous roots were produced on NAA or IAA supplemented medium and only some calli were produced on GA3 supplemented medium. The regeneration response varied with different concentrations of PGRs, singly and also in combinations. In the case of combined application of PGRs, the highest shoot regeneration (75.3%) and number of shoot per explant (11.5) and number of roots per explant (7.0) were obtained from the MS solid medium supplemented with zeatin (2 mg l-1), NAA (0.1 mg l-1) and GA3 0.1 mg l-1). Among the three types of explants evaluated, internodes produced the highest number of shoots and roots for both potato cvs. Gui valley and Bora valley, and petiole produced the least number of shoots and roots. The regenerated shoots were rooted in PGRs-free MS solid medium and successfully established under glasshouse condition. Leaf, flower, and tuber morphology were identical to in vitro control and mother plants in the same conditions. This optimized regeneration system can be used for rapid shoot proliferation and also for gene transformation.DOI: http://dx.doi.org/10.3126/njst.v12i0.6471 Nepal Journal of Science and Technology 12 (2011) 1-6 

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