scholarly journals Characterization of Physiological Resistance to White Mold and Search for Molecular Markers Linked to Resistance via Advanced Backcross QTL Analysis in an Interspecific Cross between Phaseolus coccineus and P. vulgaris

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 973B-973 ◽  
Author(s):  
J. Erron Haggard ◽  
James R. Myers
Keyword(s):  
Common Bean ◽  
Interspecific Cross ◽  
White Mold ◽  
Resistance Qtl ◽  
Additional Information ◽  
Advanced Backcross ◽  
Backcross Populations ◽  
Scarlet Runner Bean ◽  
Mold Resistance ◽  
High Level

White mold, caused by Sclerotinia sclerotiorum (Lib.) de Bary, causes major losses in dry and snap bean (Phaseolus vulgaris) production. With little genetic variation for white mold resistance in common bean, other potential sources for resistance must be investigated. Accessions of scarlet runner bean (P. coccineus) have been shown to have partial resistance exceeding any to be found in common bean. Resistance is quantitative with at least six QTL found in a P. coccineus intraspecific resistant × susceptible cross. Our goal is to transfer high levels of resistance from P. coccineus into commercially acceptable common bean lines. We developed interspecific advanced backcross populations for mapping and transfer of resistance QTL. 111 BC2F5 lines from a cross between OR91G and PI255956 have been tested in straw tests and oxalate tests, as well as in a field trial. The data show that the OR91G × PI255956 population carries a high level of resistance, but because of the quantitative nature of resistance, it may be necessary to intercross individuals to achieve higher levels. SSR, RAPD, and AFLP markers are being tested in the population to construct a linkage map for placement of QTL. QTL identified from each type of test (straw, oxalate, and field) may provide additional information about the genetic architecture of white mold resistance. Three other populations are from advanced backcrosses of the recurrent parents G122, OR91G, and MO162, with PI433251B as the donor parent in each. Analyses and advance of these populations will follow, the results of which should confirm QTL identified in the OR91G × PI255956 population, as well as possible additional resistance QTL from PI433251B.

Identification of QTL Associated with White Mold Resistance in Common Bean

Crop Science ◽  
2005 ◽  
Vol 45 (6) ◽  
pp. 2482-2490 ◽  
Author(s):  
Marcio Ender ◽  
James D. Kelly
Keyword(s):  
Common Bean ◽  
White Mold ◽  
Mold Resistance

Large-scale density-based screening for pea weevil resistance in advanced backcross lines derived from cultivated field pea (Pisum sativum) and Pisum fulvum

2012 ◽  
Vol 63 (7) ◽  
pp. 612 ◽  
Author(s):  
N. Aryamanesh ◽  
O. Byrne ◽  
D. C. Hardie ◽  
T. Khan ◽  
K. H. M. Siddique ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Large Scale ◽  
Interspecific Cross ◽  
Field Pea ◽  
Separation Method ◽  
Density Separation ◽  
Advanced Backcross ◽  
Pisum Fulvum ◽  
Resistant Lines ◽  
Backcross Populations

The pea weevil, Bruchus pisorum, is one of the most intractable pest problems of cultivated field pea (Pisum sativum) in the world. Pesticide application, either as a contact insecticide spray to the field pea crop or fumigation of the harvested seed, is the only available method for its control. The aim of the study was to develop a quick and reliable method to screen for pea weevil resistance and increase efficiency in breeding for this important trait. Backcrossed progenies derived from an interspecific cross between cultivated field pea and its wild relative (Pisum fulvum, source of resistance for pea weevil) were subjected to natural infestation in field plots. Mature seeds were hand-harvested, stored to allow development of adult beetles, and then separated into infested and non-infested using a density separation method in 30% caesium chloride (CsCl). Susceptibility and resistance of the progenies were calculated based on this method and further confirmed by a glasshouse bioassay. Resistance in backcross populations improved considerably through selection of resistant lines using the density separation method. We found that the method using CsCl separation is a useful tool in breeding for pea weevil resistance. We were able to introgress pea weevil resistance from P. fulvum into cultivated field pea through backcrossing to produce several advanced pea weevil resistant lines following this procedure.

scholarly journals 061 Examining the Phaseolus coccineus Collection for White Mold Resistance

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 399A-399 ◽  
Author(s):  
Barbara Gilmore ◽  
James R. Myers
Keyword(s):  
Field Test ◽  
Phaseolus Coccineus ◽  
White Mold ◽  
Small Lesion ◽  
Dry Bean ◽  
Blue Lake ◽  
Total Plant ◽  
Physiological Resistance ◽  
Scarlet Runner Bean ◽  
Mold Resistance

White mold, (Sclerotinia sclerotiorum), is an aggressive pathogen of beans and is capable of inflicting devastating damage on yield. Finding resistance is a major concern to bean breeders. The scarlet runner bean (Phaseolus coccineus) is generally known to have greater resistance to white mold than does the common bean, (P. vulgaris). Since it is possible to cross these two species, we have started to examine the NPGS core collection of P. coccineus for resistance to this pathogen. A straw test was used to measure physiological resistance of bean stems to white mold. A rating of one equates to a small lesion, resulting from contact with inoculum, and a rating of nine describes total plant collapse. Controls that were used were two common beans, 91G, a commercially produced, blue lake type snap bean and ExRico, a small, white dry bean. The bean 91G received a straw test rating of 8.3, which correlates to a field test rating of 8.5. ExRico rated 7.4 with the straw test and had a field test score of 6.5. Within the P. coccineus collection we found very strong resistance, with straw test values of 1 and 2 in several individual plants and in some accessions. Accessions that had individuals that displayed the strongest resistance of all the plants tested were: PI201299, PI361302, PI406938, and PI535278. These accessions appeared to be segregating for white mold resistance. Accessions showing the best average resistance were: PI313221, PI361372, PI361539, and PI583553. Because P. coccineus is outcrossed, we expected to find variation within accessions for white mold resistance. Some accessions had uniformly high levels of resistance, while other accessions showed variability.

Large-seeded Common Bean PRA152, PRA154, and PRA155 with High Levels of Broad-Spectrum White Mold Resistance

2017 ◽  
Vol 11 (3) ◽  
pp. 305-310 ◽  
Author(s):  
Shree P. Singh ◽  
Howard F. Schwartz ◽  
Henry Terán ◽  
Carlos Centeno ◽  
Kristen Otto
Keyword(s):  
Common Bean ◽  
Broad Spectrum ◽  
White Mold ◽  
Mold Resistance

Introgressing White Mold Resistance from Phaseolus Species of the Secondary Gene Pool into Common Bean

Crop Science ◽  
2009 ◽  
Vol 49 (5) ◽  
pp. 1629-1637 ◽  
Author(s):  
Shree P. Singh ◽  
Henry Terán ◽  
Howard F. Schwartz ◽  
Kristen Otto ◽  
Margarita Lema
Keyword(s):  
Common Bean ◽  
Gene Pool ◽  
White Mold ◽  
Mold Resistance ◽  
Phaseolus Species

scholarly journals Inheritance of White Mold Resistance in Phaseolus vulgaris × P. coccineus Crosses

Plant Disease ◽  
2006 ◽  
Vol 90 (9) ◽  
pp. 1167-1170 ◽  
Author(s):  
Howard F. Schwartz ◽  
Kristen Otto ◽  
Henry Terán ◽  
Margarita Lema ◽  
Shree P. Singh
Keyword(s):  
Phaseolus Vulgaris ◽  
Dominant Gene ◽  
The United States ◽  
White Mold ◽  
Interspecific Crosses ◽  
Dry Bean ◽  
Breeding Lines ◽  
Fort Collins ◽  
Scarlet Runner Bean ◽  
Mold Resistance

The fungus Sclerotinia sclerotiorum, cause of white mold, is known to attack >400 plant species. It is a widespread problem in dry bean (Phaseolus vulgaris) in the United States, causing >30% average yield losses. Low to moderate levels of resistance are found in dry bean. However, some accessions of P. coccineus (commonly known as scarlet runner bean) possess a relatively higher level of resistance. Our objective was to verify the reaction of 13 known white mold-resistant P. coccineus germ plasms and determine inheritance of resistance in accessions PI 433246 and PI 439534. Pinto Othello was crossed with PI 433246, and the resulting interspecific F1 was back-crossed onto Othello and allowed to produce F2 seed. Similarly, pinto UI 320 was crossed with PI 439534. The F1 was backcrossed onto UI 320 and allowed to produce F2 seed. The two parents, F1, F2, and backcross to dry bean of each set were evaluated in the greenhouse using the straw test at Fort Collins, CO in 2004. All 13 P. coccineus accessions and the two F2 also were evaluated using the modified petiole test at Kimberly, ID in 2005. All 13 P. coccineus accessions were variable in a 2002 straw test when rated for white mold reaction on a 1-to-9 scale, because the mean disease score ranged from 1.9 for PI 433246 to 4.4 for PI 189023 and 8.8 for the susceptible check Bill Z. For the petiole test, when rated on a 1-to-9 scale, the accessions exhibited an intermediate white mold score of 4 or 5 in 2005. In 2004, the susceptible check Othello exhibited a mean score of 7.9 compared with 3.4, 3.2, and 2.1 for PI 433246, UI 320, and PI 439534, respectively. The white mold reaction of PI 433246 and PI 439534 was dominant in their respective F1. The F2 segregation further indicated that white mold resistance in PI 433246 and PI 439534 was controlled by a single dominant gene. These two and other white mold-resistant P. coccineus accessions and selected breeding lines from the interspecific crosses should be useful for future improvement of white mold resistance of pinto and other market classes of dry and green or snap bean.

scholarly journals White mold resistance-associated quantitative trait loci in the Jalo x Small White common bean population

2016 ◽  
Vol 15 (3) ◽  
Author(s):  
D.A. Souza ◽  
M. Balestre ◽  
A.K.A. Pamplona ◽  
M.E. Leite ◽  
J.A. Dias ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Common Bean ◽  
Quantitative Trait ◽  
White Mold ◽  
Trait Loci ◽  
Mold Resistance

Linked candidate genes of different functions for white mold resistance in common bean (Phaseolus vulgaris. L) are identified by QTL-based pooled sequencing

2019 ◽  
Author(s):  
Atena Oladzadabbasabadi ◽  
Sujan Mamidi ◽  
Phillip N. Miklas ◽  
Rian Lee ◽  
Phillip McClean
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Candidate Genes ◽  
White Mold ◽  
Potential Candidate ◽  
Sequencing Analysis ◽  
Phaseolus Vulgaris L ◽  
Pooled Sequencing ◽  
Physiological Resistance ◽  
Mold Resistance

Abstract Background White mold (WM) is a major disease in common bean ( Phaseolus vulgaris L.), and its complex quantitative genetic control has limited the development of WM resistant cultivars. WM2.2 is one of the nine meta-QTL that has a major effect on WM tolerance. This QTL explains up to 35% of the phenotypic variation and was previously mapped to a large interval on Pv02. Our objective was to narrow the interval of this QTL using QTL-based bulk segregant analysis.Results The phenotypic and genotypic data from two RIL populations (R31 and Z0726-9), which possess different genetic backgrounds for white mold resistance, were used to select resistant and susceptible lines to generate subpopulations for bulk DNA sequencing, and reads were aligned against the sequence of the resistance parent. The QTL physical intervals for each RIL population were mapped by fixed SNPs in 10kb-2kb sliding windows. WM2.2 QTL was split into two regions WM2.2a (3.54-4.56 Mbp; euchromatic) and WM 2.2b (12.19 to 26.41 Mbp; heterochromatic) in populations R31 and Z0726-9, respectively. For each QTL interval, the possible functional contribution of significant non-synonymous and synonymous polymorphisms was investigated. Gene models encoding for pentatricopeptide repeat, gibberellin 2-oxidase, and heat-shock proteins are the likely candidate genes associated with WM2.2a resistance. A TIR-NBS-LRR class of disease resistance protein and a EF-TU receptor are potential candidate genes associated with WM2.2b resistance and most likely trigger a physiological resistance response to WM.Conclusion QTL-based pooled sequencing analysis revealed that the large genomic region associated with WM2.2 meta QTL consists of two major QTL each associated with a different WM resistance function. WM2.2a region is most likely associated with avoidance mechanisms while WM2.2b region triggers physiological resistance.

Quantitative Trait Loci Linked to White Mold Resistance in Common Bean

Crop Science ◽  
2007 ◽  
Vol 47 (6) ◽  
pp. 2285-2294 ◽  
Author(s):  
Judd J. Maxwell ◽  
Mark A. Brick ◽  
Patrick F. Byrne ◽  
Howard F. Schwartz ◽  
Xueyan Shan ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Common Bean ◽  
Quantitative Trait ◽  
White Mold ◽  
Trait Loci ◽  
Mold Resistance

Comparative QTL Map for White Mold Resistance in Common Bean, and Characterization of Partial Resistance in Dry Bean Lines VA19 and I9365-3

Crop Science ◽  
2011 ◽  
Vol 51 (1) ◽  
pp. 123-139 ◽  
Author(s):  
Marilyn Soule ◽  
Lyndon Porter ◽  
Juliana Medina ◽  
Gloria P. Santana ◽  
Matthew W. Blair ◽  
...  
Keyword(s):  
Common Bean ◽  
Partial Resistance ◽  
White Mold ◽  
Dry Bean ◽  
Qtl Map ◽  
Mold Resistance
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