Linked candidate genes of different functions for white mold resistance in common bean (Phaseolus vulgaris. L) are identified by QTL-based pooled sequencing

2019 ◽  
Author(s):  
Atena Oladzadabbasabadi ◽  
Sujan Mamidi ◽  
Phillip N. Miklas ◽  
Rian Lee ◽  
Phillip McClean
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Candidate Genes ◽  
White Mold ◽  
Potential Candidate ◽  
Sequencing Analysis ◽  
Phaseolus Vulgaris L ◽  
Pooled Sequencing ◽  
Physiological Resistance ◽  
Mold Resistance

Abstract Background White mold (WM) is a major disease in common bean ( Phaseolus vulgaris L.), and its complex quantitative genetic control has limited the development of WM resistant cultivars. WM2.2 is one of the nine meta-QTL that has a major effect on WM tolerance. This QTL explains up to 35% of the phenotypic variation and was previously mapped to a large interval on Pv02. Our objective was to narrow the interval of this QTL using QTL-based bulk segregant analysis.Results The phenotypic and genotypic data from two RIL populations (R31 and Z0726-9), which possess different genetic backgrounds for white mold resistance, were used to select resistant and susceptible lines to generate subpopulations for bulk DNA sequencing, and reads were aligned against the sequence of the resistance parent. The QTL physical intervals for each RIL population were mapped by fixed SNPs in 10kb-2kb sliding windows. WM2.2 QTL was split into two regions WM2.2a (3.54-4.56 Mbp; euchromatic) and WM 2.2b (12.19 to 26.41 Mbp; heterochromatic) in populations R31 and Z0726-9, respectively. For each QTL interval, the possible functional contribution of significant non-synonymous and synonymous polymorphisms was investigated. Gene models encoding for pentatricopeptide repeat, gibberellin 2-oxidase, and heat-shock proteins are the likely candidate genes associated with WM2.2a resistance. A TIR-NBS-LRR class of disease resistance protein and a EF-TU receptor are potential candidate genes associated with WM2.2b resistance and most likely trigger a physiological resistance response to WM.Conclusion QTL-based pooled sequencing analysis revealed that the large genomic region associated with WM2.2 meta QTL consists of two major QTL each associated with a different WM resistance function. WM2.2a region is most likely associated with avoidance mechanisms while WM2.2b region triggers physiological resistance.

scholarly journals Genome-Environment Association Analysis for Bio-Climatic Variables in Common Bean (Phaseolus vulgaris L.) from Brazil

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1572
Author(s):  
Júlio Cesar F. Elias ◽  
Maria Celeste Gonçalves-Vidigal ◽  
Andrea Ariani ◽  
Giseli Valentini ◽  
Maria da Conceição Martiniano-Souza ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Candidate Genes ◽  
Climatic Variables ◽  
Limiting Factor ◽  
Gene Pools ◽  
Sequencing Analysis ◽  
Phaseolus Vulgaris L ◽  
Population Structure Analysis ◽  
Bioclimatic Variables

Abiotic stress is a limiting factor for common bean (Phaseolus vulgaris L.) production globally. The study of the genotypic, phenotypic, and bio-climatic variables in a broad set of accessions may assist the identification of genomic regions involved in the climatic adaptation of the common bean. We conducted a genotyping-by-sequencing analysis using 28,823 SNPs on 110 georeferenced common bean accessions from Brazil to discover associations between SNPs and bio-climatic indexes. The population structure analysis clustered the accessions into two groups corresponding to the Andean and Mesoamerican gene pools. Of the 19 bioclimatic variables, 17 exhibited a significant association with SNPs on chromosomes Pv01, Pv02, Pv03, Pv04, Pv06, Pv09, Pv10, and Pv11 of common bean. Ten candidate genes were associated with specific bio-climatic variables related to temperature and precipitation. The candidate genes associated with this significant Pv09 region encode a Platz transcription factor family protein previously reported to be an essential regulator of drought stress. The SNP markers and candidate genes associated with the bio-climatic variables should be validated in segregating populations for water stress, which could further be used for marker-assisted selection. As a result, bean breeding programs may be able to provide advances in obtaining drought-tolerant cultivars.

Efficacy of three greenhouse screening methods for the identification of physiological resistance to white mold in dry bean

2009 ◽  
Vol 89 (4) ◽  
pp. 755-762 ◽  
Author(s):  
H Terán ◽  
S P Singh
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Sclerotinia Sclerotiorum ◽  
Screening Method ◽  
White Mold ◽  
Screening Methods ◽  
Post Inoculation ◽  
Dry Bean ◽  
Physiological Resistance ◽  
Nueva Granada

White mold (WM) caused by Sclerotinia sclerotiorum (Lib.) de Bary is the most devastating disease of common bean (dry and snap or garden bean) (Phaseolus vulgaris L.) in North America. The use of a reliable screening method (SM) in common bean is crucial to improve physiological resistance to WM. The objective of this study was to compare the efficacy of three SM to identify physiological resistance in dry bean genotypes with different evolutionary origins and levels of resistance. Screening methods tested were: (i) the modified straw test or cut–stem (CSM); (ii) infected bean flower (IFL); and (iii) infected oat seed (IOS). A 195, ICA Bunsi, Othello, and VCW 54 dry bean were tested with the three SM. The experimental design was a split plot in randomized complete blocks with three replications in 2007 and 2008. Two independent inoculations 1 wk apart for each SM were made. The WM reaction was scored at 16, 23, and 33 d post-inoculation (DPI) using a 1 to 9 scale. There were highly significant differences between SM and its interaction with years. The CSM and IFL were the most consistent and highly correlated (r > 0.70, P < 0.01). Interspecific breeding line VCW 54 consistently had the highest WM resistance across years, SM, and evaluation dates, followed by A 195. White mold scores increased with delayed evaluations. Thus, CSM or IFL with disease assessed 33 DPI should be used for identifying common bean genotypes with high levels of physiological resistance to WM.Key words: Common bean, growth habit, race Mesoamerica, race Nueva Granada, Phaseolus vulgaris, Sclerotinia sclerotiorum

Identification of candidate genes associated with CBB resistance in common bean HR45 (Phaseolus vulgaris L.) using cDNA-AFLP

2010 ◽  
Vol 38 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Chun Shi ◽  
Sarita Chaudhary ◽  
Kangfu Yu ◽  
Soon J. Park ◽  
Alireza Navabi ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Candidate Genes ◽  
Phaseolus Vulgaris L

Inheritance of seed phytate and phosphorus levels in common bean (Phaseolus vulgaris L.) and association with newly-mapped candidate genes

2012 ◽  
Vol 30 (3) ◽  
pp. 1265-1277 ◽  
Author(s):  
Matthew W. Blair ◽  
Andrea Lorena Herrera ◽  
Tito Alejandro Sandoval ◽  
Gina Viviana Caldas ◽  
Marizia Filleppi ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Candidate Genes ◽  
Phaseolus Vulgaris L ◽  
Phosphorus Levels

Bacillus sp. FSQ1: a Promising Biological Control Agent Against Sclerotinia sclerotiorum, the Causal Agent of white Mold in Common Bean (Phaseolus vulgaris L.)

2021 ◽  
Vol 48 (6) ◽  
pp. 729-739
Author(s):  
María Fernanda Villarreal-Delgado ◽  
Fannie Isela Parra-Cota ◽  
Luis Alberto Cira-Chávez ◽  
María Isabel Estrada-Alvarado ◽  
Sergio de los Santos-Villalobos
Keyword(s):  
Biological Control ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Sclerotinia Sclerotiorum ◽  
Biological Control Agent ◽  
Control Agent ◽  
Causal Agent ◽  
White Mold ◽  
Bacillus Sp ◽  
Phaseolus Vulgaris L

scholarly journals Incidence and severity of white mold in common bean submitted to different cultivation practices

2017 ◽  
Vol 21 (10) ◽  
pp. 691-696 ◽  
Author(s):  
David R. P. Montes ◽  
Rubens A. Oliveira ◽  
Luan B. Giovanelli ◽  
Reginaldo M. de Oliveira ◽  
Júlio C. M. Baptestini ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Block Design ◽  
White Mold ◽  
Phaseolus Vulgaris L ◽  
Main Method ◽  
Randomized Block Design ◽  
Cultivation Practices ◽  
Favorable Conditions

ABSTRACT Due to the socioeconomic importance of common bean in Brazil, studies that allow establishing favorable conditions for not compromising the crop with white mold, in view of the enormous damages caused by this fungus, become vital to Brazilian agriculture. Therefore, the aim of this study was to evaluate the incidence and severity of white mold in common bean (Phaseolus vulgaris L.), cultivar ‘Madrepérola’, subjected to different irrigation intervals, planting densities and fungicide application. The research was conducted in Viçosa-MG, Brazil, in the years 2011 and 2012. The statistical analysis used the split-split-plot scheme. The fungicide applications (with or without fungicide) were allocated to plots, the irrigation intervals (3, 6, 9 and 12 days) to sub-plots and planting densities (6, 9, 12 and 15 plants per linear meter) to sub-subplots, in a randomized block design with three replicates. The variation of the irrigation intervals did not contribute significantly to the control of white mold. It is recommended to use lower planting densities in order to minimize the damages caused by the disease. The use of the fungicide was the main method of white mold control in two consecutive years.

scholarly journals Effectiveness of Moringa oleifera (Lam) Extracts against Sclerotinia sclerotorium (Lib) De Bary, the Causative Agent of White Mold of Common Bean (Phaseolus vulgaris L.)

2020 ◽  
Vol 9 (11) ◽  
pp. 812-820
Author(s):  
Atindo Songwe Thierry ◽  
Ndongo Bekolo ◽  
Kuate Tueguem William Norbert ◽  
Ngatsi Zemko Patrice ◽  
Manga Anaba Désiré ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Causative Agent ◽  
Moringa Oleifera ◽  
White Mold ◽  
Phaseolus Vulgaris L ◽  
Moringa Oleifera Lam
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