scholarly journals Efficient Propagation Technique of Euphorbia ×lomi Thai Hybrids

HortScience ◽  
2009 ◽  
Vol 44 (2) ◽  
pp. 495-498 ◽  
Author(s):  
Giancarlo Fascella ◽  
Giovanvito Zizzo
Keyword(s):  
Acetic Acid ◽  
Root Zone ◽  
Naphthalene Acetic Acid ◽  
Rooted Cuttings ◽  
Potted Plants ◽  
Coir Dust ◽  
Mother Plants ◽  
Propagation Technique ◽  
Rooting Capacity

A simple and efficient in vivo propagation protocol of two cultivars of Euphorbia ×lomi Rauh is described. Mother plants of ‘Nguen Muang’ and ‘Porn Ying Yai’ were soilless grown in 1 coconut coir dust:1 perlite (by volume) and 2 sphagnum peat:1 perlite (by volume) to produce cuttings. Plants cultured in peat-based substrate showed thicker stems (36.0 versus 30.5 mm, respectively), higher production of cuttings (10.4 versus 5.6 per plant), higher water absorption (265.7 versus 153.5 mL/plant/d), and higher content of nutrients in the root zone as compared with those cultivated in coir dust mixture. Cuttings harvested from 1-year-old mother plants of both cultivars were treated with 4000 ppm of α-naphthalene acetic acid (NAA) powder and inserted into bottom-heated benches filled with different substrates [perlite, 1 perlite:1 coir dust (by volume), coir dust] for rooting. Cuttings in pure perlite evidenced higher rooting percentage (86.2%) and longer roots (6.2 cm) than those in other media. Application of NAA resulted in higher rooting capacity (78.8 versus 68.3%, respectively) and higher amount of roots per cutting (13.4 versus 10.2) than treatment without auxin. Rooted cuttings grown in 1 peat:1 perlite medium (by volume) produced higher marketable potted plants than in 2 peat:1 perlite medium.

scholarly journals Influence of phytohormones on vegetative propagation of different forms of pannonian thyme (Thymus pannonicus All.)

2020 ◽  
Vol 26 (2) ◽  
pp. 39-52
Author(s):  
Vladimir Filipović ◽  
Vladan Ugrenović ◽  
Zoran Maksimović ◽  
Vera Popović ◽  
Danica Paunović ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Root Hairs ◽  
Morphological Properties ◽  
Naphthalene Acetic Acid ◽  
Plant Establishment ◽  
Rooted Cuttings ◽  
Average Percentage ◽  
Plant Collection ◽  
Rooting Of Cuttings ◽  
The Impact

The paper examined the impact of the application of three different phytohormones (INCIT 2, INCIT 5 and INCIT 8, all based on a-Naphthalene Acetic Acid (NAA)), with the cuttings of two forms of Pannonian thyme (the L-16 form, with hairy leaves, and the L-9 form, with hairless leaves), established during two periods (March and May), on the percentage of the rooted cuttings and the morphological properties of the seedlings. As the control, the variant without the application of the phytohormones was taken. The research was conducted in the period from 2019 to 2020, in a plastic greenhouse, using the plant collection of the Institute for Medicinal Plants Research "Dr Josif Pančić", which is located in Pančevo city, Serbia (44°52'20"N; 20°42'06"E; 74 m.a.s.l.). For this research was used the Pannonian thyme species (Thymus pannonicus All.) from the Lamiaceae family, which is grown and produced at the Institute. Plant establishment was carried out in four repetitions with 33 cuttings per each variant. Standard measures of care were used during the period of production of Pannonian thyme. The measurement was performed after 60 days from the date of conducted the experiment. The following parameters were measured: the percentage of rooted cuttings (%), the mass of the rooted plantlings (g), the mass of roots (g), the length of rooting (cm) and the number of root hairs of the formed rootings. The measuring was done using a ruler, millimetre paper and an analytical balance. Given the results achieved, INCIT 2 proved to be the most suitable phytohormone for the rooting of Pannonic thyme cuttings, with the average percentage of rooted cuttings of 61.3%. The lowest rooting percentage was recorded in the control variant, only 29.4%. Satisfactory rooting was found in the cuttings treated with INCIT 8 (57.6%). The L-16 form cuttings showed a higher rooting rate, an average of 53.8%, whereas the L-9 form cuttings had a lower average percentage of rooted cuttings (45.0%) for both plant establishment periods. Greater success and quality in the rooting of cuttings was recorded in the second (May) period of plant establishment, averaging 58.1%, which was higher by 17.4% than the percentage of rooted cuttings in the first establishment period (40.7%). It is important to note that the rooting of the L-9 form was significantly higher in the second establishment period (58.1%) than that in the first establishment period (31.9%).

scholarly journals Rooted cuttings by IBA (Indole butyric acid) and NAA (Naphthalene acetic acid) in black pepper (piper nigrum) - A case study in Vietnam

2020 ◽  
Vol 8 (3) ◽  
pp. 1880-1885
Author(s):  
Tran Quyen Nguyen ◽  
Ba Huy Nguyen ◽  
Dieu Hien Tran Thi ◽  
Oanh Duong Thi ◽  
Quang Ngoc Nguyen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Naphthalene Acetic Acid ◽  
Indole Butyric Acid ◽  
Rooted Cuttings

scholarly journals Hormone-injected leaf cutting, a new efficient in vivo multiplication protocol for two succulent plants

2017 ◽  
Author(s):  
Xiaodan Xu ◽  
Wei Zheng
Keyword(s):  
Acetic Acid ◽  
Commercial Production ◽  
The Other ◽  
Naphthalene Acetic Acid ◽  
Shoot Induction ◽  
Induction Frequency ◽  
Leaf Cutting ◽  
Succulent Plants

This study aimed to establish a simple and efficient in vivo multiplication protocol by leaf cutting to satisfy the supply of young succulent ornamentals Pachyveria pachytoides and Sedum morganianum. The regenerability of leaves injected with 6-benzylaminopurine (BAP) and α-naphthalene acetic acid (NAA) in vivo were tested with common leaf cutting as control. Results showed a 100% shoot induction frequency using hormone-injeceted methods for the two species. The number of shoots per leaf of 4.0 or 6.0 mg l −1 BAP and 0.1 mg l −1 NAA injected in vivo (5.08-5.14 in P. pachytoides, and 6.22-6.74 for S. morganianum) were significantly greater than that of the other treatments. Since the h ormone-injected leaf cutting needs no aseptic operation which is necessary for in vitro multiplication, it is simple for the commercial production of the two species. The new in vivo propagation method would be of great interest for growers and breeders of succulent plants.

scholarly journals 599 Flooding Effects on Stomatal Conductance and Leaf Color Vary among Genotypes of Red and Freeman Maples

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 550C-550
Author(s):  
James A. Zwack ◽  
William R. Graves ◽  
Alden M. Townsend
Keyword(s):  
Stomatal Conductance ◽  
Root Zone ◽  
Numerical Data ◽  
Control Treatment ◽  
Blue Color ◽  
Leaf Color ◽  
Similar Reduction ◽  
Rooted Cuttings ◽  
Potted Plants

We compared two putative Freeman maples [`Jeffersred', (Autumn Blaze ®) and `Indian Summer'] and five red maples [`Franksred' (Red Sunset ®), `Autumn Flame', `PNI 0268' (October Glory®), `Fairview Flame', and unnamed selection 59904] for effects of flooding on stomatal conductance. A method for quantifying changes in leaf color that occurred on flooded plants also was developed. Potted plants grown from rooted cuttings in a greenhouse were subjected to 75 days of root-zone inundation (flood treatment) or were irrigated frequently (control treatment). Across genotypes, stomatal conductance of flooded plants initially increased by about 20% and then fell to and was sustained below 50 mmol·s–1·m–2. Stomatal conductance of flooded plants of `Indian Summer' decreased to 20 mmo·s–1·m–2 after 8 days of inundation, and two of three flooded `Indian Summer' plants died during treatment. Other genotypes required at least twice this time to display a similar reduction in stomatal conductance, indicating `Indian Summer' may be particularly flood sensitive. Intensities of red, green, and blue color at a consistent interveinal position were analyzed with Visilog software by using scanned leaf images of the youngest fully expanded leaf of each plant in both treatments. A genotype × irrigation interaction existed for the ratio of green to red intensity. This method provided numerical data that corresponded well to differences among genotypes we observed visually. For example, while flooding did not alter the color of `Autumn Flame' leaves, the ratio of green to red was three times greater for controls of Autumn Blaze® than for the flooded plants of this cultivar.

Morphological evaluation and antioxidant activity of in vitro- and in vivo-derived Echinacea purpurea plants

2012 ◽  
Vol 7 (4) ◽  
pp. 698-707 ◽  
Author(s):  
Ely Zayova ◽  
Ira Stancheva ◽  
Maria Geneva ◽  
Maria Petrova ◽  
Rumiana Vasilevska-Ivanova
Keyword(s):  
Acetic Acid ◽  
Basal Medium ◽  
Echinacea Purpurea ◽  
Water Soluble ◽  
Cultivated Plants ◽  
Naphthalene Acetic Acid ◽  
High Survival ◽  
Ms Medium

AbstractAn effective in vitro protocol for rapid clonal propagation of Echinacea purpurea (L.) Moench through tissue culture was described. The in vitro propagation procedure consisted of four stages: 1) an initial stage - obtaining seedlings on Murashige and Skoog (MS) basal medium with 0.1 mg L−1 6-benzylaminopurine, 0.1 mg L−1 α-naphthalene acetic acid and 0.2 mg L−1 gibberellic acid; 2) a propagation stage — shoot formation on MS medium supplemented with 1 mg L−1 6-benzylaminopurine alone resulted in 9.8 shoots per explant and in combination with 0.1 mg L−1 α-naphthalene acetic acid resulted in 16.2 shoots per explant; 3) rooting stage — shoot rooting on half strength MS medium with 0.1 mg L−1 indole-3-butyric acid resulted in 90% rooted microplants; 4) ex vitro acclimatization of plants. The mix of peat and perlite was the most suitable planting substrate for hardening and ensured high survival frequency of propagated plants. Significant higher levels were observed regarding water-soluble and lipid-soluble antioxidant capacities (expressed as equivalents of ascorbate and α-tocopherol) and total pnenols content in extracts of Echinaceae flowers derived from in vitro propagated plants and adapted to field conditions in comparison with traditionally cultivated plants.

scholarly journals Hormone-injected leaf cutting, a new efficient in vivo multiplication protocol for two succulent plants

2017 ◽  
Author(s):  
Xiaodan Xu ◽  
Wei Zheng
Keyword(s):  
Acetic Acid ◽  
Commercial Production ◽  
The Other ◽  
Naphthalene Acetic Acid ◽  
Shoot Induction ◽  
Induction Frequency ◽  
Leaf Cutting ◽  
Succulent Plants

This study aimed to establish a simple and efficient in vivo multiplication protocol by leaf cutting to satisfy the supply of young succulent ornamentals Pachyveria pachytoides and Sedum morganianum. The regenerability of leaves injected with 6-benzylaminopurine (BAP) and α-naphthalene acetic acid (NAA) in vivo were tested with common leaf cutting as control. Results showed a 100% shoot induction frequency using hormone-injeceted methods for the two species. The number of shoots per leaf of 4.0 or 6.0 mg l −1 BAP and 0.1 mg l −1 NAA injected in vivo (5.08-5.14 in P. pachytoides, and 6.22-6.74 for S. morganianum) were significantly greater than that of the other treatments. Since the h ormone-injected leaf cutting needs no aseptic operation which is necessary for in vitro multiplication, it is simple for the commercial production of the two species. The new in vivo propagation method would be of great interest for growers and breeders of succulent plants.

FTIR, GC-MS and HPLC analysis of Baliospermum montanum (Willd.) Muell. Arg.

2020 ◽  
Vol 11 (4) ◽  
pp. 5059-5066
Author(s):  
Sushma B K ◽  
Raveesha H R
Keyword(s):  
Acetic Acid ◽  
Quantitative Estimation ◽  
Chemical Constituents ◽  
Standard Procedure ◽  
Naphthalene Acetic Acid ◽  
Root Extract ◽  
Pentanoic Acid ◽  
Trimethylsilyl Ester ◽  
Isolation And Characterization ◽  
Ms Analysis

The present work is aimed to determine the chemical constituents in Baliospermum montanum methanolic extracts. An in vitro regenerated procedure was developed for the induction of callus from stem explant cultured on Murashige and Skoog (MS) medium fortified with various concentration and permutations of 2, 4-dichloro phenoxy acetic acid, 1-naphthalene acetic acid, 6-benzyl amino purine and gibberellic acid. FTIR & GC-MS analysis was done according to standard procedure. The quantitative estimation of β-sitosterol was done by HPLC method. Maximum fresh and dry weight of callus was estimated in the combination of GA3 (0.5 mg/L) + NAA (2 mg/L) compared to other concentration. The FTIR analysis showed various functional compounds with different characteristic peak values in the extracts. Major bioactive constituents were recognized in the GC-MS analysis. Root extract revealed the existence of 1-hexadecanol, pentanoic acid, 2-(aminooxy)- and 1-hexacosanol. Leaf extract showed the presence of propanoic acid, 2-oxo-, trimethylsilyl ester, 9,12-octadecadienoic acid (z,z)-, trimethylsilyl ester, docosane, 1,22-dibromo- and pentatriacontane. Stem and stem derived callus exhibit the presence of 1,6,3,4-dihydro-2-deoxy-beta-d-lyxo-hexopyranose, n-hexadecanoic acid and pentanoic acid, 2-(aminooxy). The methanolic extract of leaf exhibited 0.2149 % of β-sitosterol content. There were no peaks observed in the root, stem and stem derived callus. Further studies are necessary for the isolation and characterization of bioactive compounds from B. montanum.

PENGARUH KOMBINASI INTENSITAS NAUNGAN DENGAN ZAT PENGATUR TUMBUH INDOLE BUTIRIC ACID (IBA), NAPHTHALENE ACETIC ACID (NAA), DAN VITAMIN B1 DALAM AKLIMATISASI PERTUMBUHAN BIBIT GAHARU (Aquilaria beccariana)

2013 ◽  
Vol 14 (3) ◽  
Author(s):  
Daru Mulyono
Keyword(s):  
Acetic Acid ◽  
Factorial Design ◽  
Vitamin B1 ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Optimal Formula

The objective of this research is to know the optimal formula of Indole Butiric Acid (IBA), Naphthalene Acetic Acid (NAA), Vitamine B1 and the combination with shading intensities to the acclimatization of Gaharu stump (Aquilaria beccariana). This research used Factorial Design with basic analysis of Complete Randomized Design in order to know theeffect of treatment. The research was carried out in Agroindustry and Biotechnology Laboratory, Ciampea, Bogor, from July to September 2007. The results of the research showed that after 8 weeks of treatment: (a). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 10 mg/l + Vitamine B1 1 mg/l was the best formula for increasingheight of Gaharu stump 4.660 cm. (b). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 30 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sum of Gaharu leaf stump 12.337 leafs, (c). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sumof Gaharu root stump 3.783 roots, and (d). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing length of Gaharu root stump 3.686 cm.

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