scholarly journals Rooted cuttings by IBA (Indole butyric acid) and NAA (Naphthalene acetic acid) in black pepper (piper nigrum) - A case study in Vietnam

2020 ◽  
Vol 8 (3) ◽  
pp. 1880-1885
Author(s):  
Tran Quyen Nguyen ◽  
Ba Huy Nguyen ◽  
Dieu Hien Tran Thi ◽  
Oanh Duong Thi ◽  
Quang Ngoc Nguyen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Naphthalene Acetic Acid ◽  
Indole Butyric Acid ◽  
Rooted Cuttings

scholarly journals Growth Response of Two Pepper (Piper nigrum L.) Stem Cuttings on Application of IBA (Indole Butyric Acid) and NAA (Naphthalene Acetic Acid)

2018 ◽  
Vol 1 (1) ◽  
pp. 87-95
Author(s):  
Rizky Wulandari ◽  
Yaya Hasanah ◽  
Meiriani Meiriani
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Growth Response ◽  
Block Design ◽  
Primary Branch ◽  
Piper Nigrum ◽  
Naphthalene Acetic Acid ◽  
Secondary Branch ◽  
Indole Butyric Acid ◽  
Significant Difference

Using fruit branch for pepper shrub propagation is one of alternatives for an efficient pepper multiplication which usually uses underlayer cuttings. This research is aimed at finding the growth response of two pepper (Piper nigrum L.) cuttings to the administration of IBA (Indole Butyric Acid) and NAA (Naphthalene Acetic Acid). This research was conducted at the greenhouse of the Faculty of Agriculture, University of Sumatera Utara, Medan (± 32 m above sea level), from April to August 2017 using a factorial randomized block design with 2 factors, pepper branch cuttings (primary branch cuttings and secondary branch cuttings) and the administration of IBA and NAA (0+0 ppm, 2500+0 ppm, 0+2500 ppm, 1500+1000 ppm, and 1000+1500 ppm). The results show that the emerging shoot rate in the secondary branch cuttings was significantly faster than in the primary branch cuttings, but the volume of root in the primary branch cuttings is significantly larger than in the secondary branch cuttings. There was no significant difference in the administration of IBA and NAA on all observed variables. The highest interaction of shoot length was found in the  primary branch cuttings with the administration of  IBA 1500 ppm + NAA 1000 ppm and the highest percentage of root and root volume was found in the primary branch cuttings with the administration of IBA 2500 ppm + NAA 0 ppm.

scholarly journals PENGARUH PEMBERIAN IBA (Indole Butyric Acid) DAN KONSENTRASI NAA (Naphthalene Acetic Acid) TERHADAP KEBERHASILAN PENYETEKAN SIRIH MERAH (Piper crocatum Ruiz and Pav.)

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Desi Maulida ◽  
Rugayah Rugayah ◽  
Tri Dewi Andalasari
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Block Design ◽  
Naphthalene Acetic Acid ◽  
Indole Butyric Acid ◽  
Randomized Block Design ◽  
The Difference ◽  
The Given

The research was conducted at the cutting red bettle (Piper crocatum Ruiz and Pav.) to determined the effect of (1) the difference growth of cuttings of red bettle which are given IBA and without IBA, (2) the concentration of NAA on growth of cutting red bettle, (3) the concentration of NAA on growth of cutting red bettle on each given IBA. The treatment was arranged in factorial (2 x 4) in randomized block design with three replication. The first factor were without IBA (A) and the given of 1000 ppm IBA (A1). The second factor were the concentration of NAA consists of: 0 ppm (B), 1000 ppm (B1), 2000 ppm (B2), and 4000 ppm (B). The results showed that the NAA concentration of 4000 ppm produced the most number of roots on either at the node or at the base of cutting. Planting cutting red bettle which given IBA 1000 ppm was able to accelerated the time leaves open and increased the number of cutting which germinate. The mixtured of giving IBA 1000 ppm and the concentration of NAA 4000 ppm, produced the most number of cutting that germinated.

scholarly journals Propagation Techniques for a Spineless Acacia wrightii

HortScience ◽  
2005 ◽  
Vol 40 (6) ◽  
pp. 1832-1837 ◽  
Author(s):  
Donita L. Bryan ◽  
Michael A. Arnold ◽  
R. Daniel Lineberger ◽  
W. Todd Watson
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Tissue Culture Propagation

Three spineless phenotypes of Acacia wrightii G. Bentham ex A. Gray were identified with aesthetic landscape potential. Experiments in seed, cutting, grafting, and tissue culture propagation were undertaken to perpetuate this desired spineless phenotype. Germination percentages for mechanically scarified seeds ranged from 33% to 94%, however yield of spineless seedlings was low (0% to 34%). Sulfuric acid scarification for 10, 20, 30, or 60 minutes hastened and unified germination compared to nontreated seeds by 7 to 8 days. Vegetative propagation was successful for softwood cuttings. Rooting measures increased with auxin (2:1 indole butyric acid to naphthalene acetic acid) concentrations from 0 to 15000 mg·L–1, with maximum rooting percentage (70%), root number (9.2), and root length (12.4 cm) per softwood cutting at 15000 mg·L–1 auxin 8 weeks after treatment. Rooting was not successful for semi-hardwood or hardwood cuttings. Whip-and-tongue or T-bud grafting was not successful. Tissue culture of shoots from in vitro germinated seedlings indicated that shoot proliferation was greatest in Murashige and Skoog (MS) medium with 15 μm zeatin. The number of shoots that rooted in vitro increased with increasing concentrations of indole-3-butyric acid from 0 to 25 μm.

scholarly journals Influence of phytohormones on vegetative propagation of different forms of pannonian thyme (Thymus pannonicus All.)

2020 ◽  
Vol 26 (2) ◽  
pp. 39-52
Author(s):  
Vladimir Filipović ◽  
Vladan Ugrenović ◽  
Zoran Maksimović ◽  
Vera Popović ◽  
Danica Paunović ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Root Hairs ◽  
Morphological Properties ◽  
Naphthalene Acetic Acid ◽  
Plant Establishment ◽  
Rooted Cuttings ◽  
Average Percentage ◽  
Plant Collection ◽  
Rooting Of Cuttings ◽  
The Impact

The paper examined the impact of the application of three different phytohormones (INCIT 2, INCIT 5 and INCIT 8, all based on a-Naphthalene Acetic Acid (NAA)), with the cuttings of two forms of Pannonian thyme (the L-16 form, with hairy leaves, and the L-9 form, with hairless leaves), established during two periods (March and May), on the percentage of the rooted cuttings and the morphological properties of the seedlings. As the control, the variant without the application of the phytohormones was taken. The research was conducted in the period from 2019 to 2020, in a plastic greenhouse, using the plant collection of the Institute for Medicinal Plants Research "Dr Josif Pančić", which is located in Pančevo city, Serbia (44°52'20"N; 20°42'06"E; 74 m.a.s.l.). For this research was used the Pannonian thyme species (Thymus pannonicus All.) from the Lamiaceae family, which is grown and produced at the Institute. Plant establishment was carried out in four repetitions with 33 cuttings per each variant. Standard measures of care were used during the period of production of Pannonian thyme. The measurement was performed after 60 days from the date of conducted the experiment. The following parameters were measured: the percentage of rooted cuttings (%), the mass of the rooted plantlings (g), the mass of roots (g), the length of rooting (cm) and the number of root hairs of the formed rootings. The measuring was done using a ruler, millimetre paper and an analytical balance. Given the results achieved, INCIT 2 proved to be the most suitable phytohormone for the rooting of Pannonic thyme cuttings, with the average percentage of rooted cuttings of 61.3%. The lowest rooting percentage was recorded in the control variant, only 29.4%. Satisfactory rooting was found in the cuttings treated with INCIT 8 (57.6%). The L-16 form cuttings showed a higher rooting rate, an average of 53.8%, whereas the L-9 form cuttings had a lower average percentage of rooted cuttings (45.0%) for both plant establishment periods. Greater success and quality in the rooting of cuttings was recorded in the second (May) period of plant establishment, averaging 58.1%, which was higher by 17.4% than the percentage of rooted cuttings in the first establishment period (40.7%). It is important to note that the rooting of the L-9 form was significantly higher in the second establishment period (58.1%) than that in the first establishment period (31.9%).

scholarly journals Determining the Rooting Ability of Hardwood Cuttings of Blueberry (Vaccinium corymbosum L.) cv. ‘Bluecrop’ Stored in Refrigerating Conditions

2020 ◽  
Vol 8 (3) ◽  
pp. 81
Author(s):  
Sabri Braha ◽  
Petrit Rama ◽  
Agim Zajmi
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Adventitious Roots ◽  
Vaccinium Corymbosum ◽  
Mother Plant ◽  
Winter Period ◽  
Naphthalene Acetic Acid ◽  
Intact Control ◽  
Rooting Ability ◽  
Time Periods

This experiment has been carried out in order to determine the rooting ability of hardwood cuttings of the blueberry cultivar ‘Bluecrop’ collected during the deep dormant winter period in the last part of January. In order to induce the adventitious roots formation, the cuttings were stimulated using Indol Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA) in various concentrations (1500, 3000, 4500 ppm), whilst one row in each box remained intact (control).  The collection of cuttings off the mother plant at the beginning of January enable the nutrients and other matter that help the process of rooting not to move from the base of cutting towards the top and consequently they remain at the base of cutting which results with quite a high rooting percentage (up to 72.5%) compared to the cuttings collected in February with rooting percentage of 67.5% and in March (52%). In both types of substrate, turf and turf-perlite 2:1 and in different time periods when cuttings have been collected, IBA proved to be better in inducing adventitious roots compared to NAA.

scholarly journals Optimization of the micropropagation of elite adult trees of Sequoia sempervirens: forest species of interest in the Basque Country, Spain

Bionatura ◽  
2021 ◽  
Vol 6 (1) ◽  
pp. 1511-1519
Author(s):  
Alejandra Rojas Vargas ◽  
A Castander-Olarieta ◽  
P Moncaleán ◽  
I.A Montalbán
Keyword(s):  
Climate Change ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Pinus Radiata ◽  
Basque Country ◽  
Sequoia Sempervirens ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Forest Species ◽  
Forestry Sector

Forest trees are renewable sources of timber and other valuable non-timber products. Nowadays, the increase in population and demand for forestry products results in overexploitation of forestry. Therefore, there is an urgent need to produce elite plants with higher productivity under stress derived from climate change to have available to afforestation. For this reason, propagation methods should be improved to be more efficient in terms of quality and productivity. The main species planted in the Basque Country is Pinus radiata; during the last three years, Pinus radiata plantations have suffered a fungus attack affecting mainly needles until the tree's death. This crisis is caused by the combined action of two fungi of the genus Dothistroma and Lecanosticta acicola, whose expansion seems to have been enhanced by weather-related factors, such as humid and hot summers. Although we have evidence of this disease's presence in our mountains since 1942, the disease has had a speedy expansion with an aggressive effect for reasons that are not scientifically known today. For the above, Basque Country forestry sector is looking for alternative species to be used in its plantations. Part of the forestry sector considers that Sequoia sempervirens could be a good choice for plantations. Besides, its high-quality wood and its tolerance to the attack of several pathogens and other diseases derived from climate change are characteristics that could confer some advantage over other forest species. The main goal of this study was to optimize the micropropagation of adult elite trees of Sequoia sempervirens. The effect of 6-benzylaminopurine, meta-Topolin and Kinetin, and 4 types of explant in the multiplication stage were analyzed to carry out this objective. Furthermore, the effect of two types of auxins: 1-naphthalene acetic acid, indole-3-butyric acid, and a mixture of both, were evaluated on the induction of roots and their subsequent effect on the acclimatization process. The best multiplication index was obtained when 4.4 µM 6-benzylaminopurine and apical explants longer than 1.5 cm of length were used. The root induction percentage was 75% in the most responsive genotype analyzed when 4.4 µM 6-benzylaminopurine was used on the induction stage, and 50 µM of 1-naphthalene acetic acid was used for rooting. Finally, after 3 months in the greenhouse, the explants cultured with Kinetin and rooted in a culture medium with indole-3-butyric acid showed the highest acclimatization success (94%).

scholarly journals Efficient Propagation Technique of Euphorbia ×lomi Thai Hybrids

HortScience ◽  
2009 ◽  
Vol 44 (2) ◽  
pp. 495-498 ◽  
Author(s):  
Giancarlo Fascella ◽  
Giovanvito Zizzo
Keyword(s):  
Acetic Acid ◽  
Root Zone ◽  
Naphthalene Acetic Acid ◽  
Rooted Cuttings ◽  
Potted Plants ◽  
Coir Dust ◽  
Mother Plants ◽  
Propagation Technique ◽  
Rooting Capacity

A simple and efficient in vivo propagation protocol of two cultivars of Euphorbia ×lomi Rauh is described. Mother plants of ‘Nguen Muang’ and ‘Porn Ying Yai’ were soilless grown in 1 coconut coir dust:1 perlite (by volume) and 2 sphagnum peat:1 perlite (by volume) to produce cuttings. Plants cultured in peat-based substrate showed thicker stems (36.0 versus 30.5 mm, respectively), higher production of cuttings (10.4 versus 5.6 per plant), higher water absorption (265.7 versus 153.5 mL/plant/d), and higher content of nutrients in the root zone as compared with those cultivated in coir dust mixture. Cuttings harvested from 1-year-old mother plants of both cultivars were treated with 4000 ppm of α-naphthalene acetic acid (NAA) powder and inserted into bottom-heated benches filled with different substrates [perlite, 1 perlite:1 coir dust (by volume), coir dust] for rooting. Cuttings in pure perlite evidenced higher rooting percentage (86.2%) and longer roots (6.2 cm) than those in other media. Application of NAA resulted in higher rooting capacity (78.8 versus 68.3%, respectively) and higher amount of roots per cutting (13.4 versus 10.2) than treatment without auxin. Rooted cuttings grown in 1 peat:1 perlite medium (by volume) produced higher marketable potted plants than in 2 peat:1 perlite medium.

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