PENGARUH KOMBINASI INTENSITAS NAUNGAN DENGAN ZAT PENGATUR TUMBUH INDOLE BUTIRIC ACID (IBA), NAPHTHALENE ACETIC ACID (NAA), DAN VITAMIN B1 DALAM AKLIMATISASI PERTUMBUHAN BIBIT GAHARU (Aquilaria beccariana)

2013 ◽  
Vol 14 (3) ◽  
Author(s):  
Daru Mulyono
Keyword(s):  
Acetic Acid ◽  
Factorial Design ◽  
Vitamin B1 ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Optimal Formula

The objective of this research is to know the optimal formula of Indole Butiric Acid (IBA), Naphthalene Acetic Acid (NAA), Vitamine B1 and the combination with shading intensities to the acclimatization of Gaharu stump (Aquilaria beccariana). This research used Factorial Design with basic analysis of Complete Randomized Design in order to know theeffect of treatment. The research was carried out in Agroindustry and Biotechnology Laboratory, Ciampea, Bogor, from July to September 2007. The results of the research showed that after 8 weeks of treatment: (a). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 10 mg/l + Vitamine B1 1 mg/l was the best formula for increasingheight of Gaharu stump 4.660 cm. (b). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 30 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sum of Gaharu leaf stump 12.337 leafs, (c). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sumof Gaharu root stump 3.783 roots, and (d). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing length of Gaharu root stump 3.686 cm.

scholarly journals Multiplikasi Eksplan Tunas Anggrek Hitam (Coelogyne pandurata Lindl.) dengan Penambahan NAA (Naphthalene Acetic Acid) dan Ekstrak Biji Jagung (Zea mays) Secara In Vitro

2021 ◽  
Vol 11 (2) ◽  
pp. 114
Author(s):  
Nurkapita Nurkapita ◽  
Riza Linda ◽  
Zulfa Zakiah
Keyword(s):  
Tissue Culture ◽  
Zea Mays ◽  
Acetic Acid ◽  
Seed Extract ◽  
Naphthalene Acetic Acid ◽  
Shoot Height ◽  
Culture Techniques ◽  
Corn Seed ◽  
Randomized Design

(Article History: Received February 18, 2021; Revised April 27, 2021; Accepted May 19, 2021) ABSTRAKPerkembangbiakan anggrek secara generatif alami membutuhkan bantuan jamur mikoriza untuk perkecambahan biji, sedangkan usaha perbanyakan konvensional memerlukan waktu lama untuk memperoleh tanaman dalam jumlah banyak. Salah satu alternatif untuk perbanyakan anggrek hitam (Coelogyne pandurata Lindl.) adalah melalui multiplikasi tunas anggrek secara in vitro. Tujuan penelitian adalah untuk membuktikan pengaruh pemberian NAA (Naphthalene Acetic Acid) dan ekstrak biji jagung (Zea mays) terhadap multiplikasi tunas anggrek hitam. Penelitian dilakukan di Laboratorium Kultur Jaringan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan dua faktor perlakuan. Faktor pertama adalah NAA terdiri dari 5 taraf konsentrasi yaitu A0 (0 M/ kontrol) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) dan A4 (5x10-6 M ) dan faktor ekstrak biji jagung (B) dengan 5 taraf konsentrasi yaitu B0 (0%), B1 (2,5%), B2 (5%); B3 (7,5%) dan B4 (10%). Pemberian kombinasi NAA dan ekstrak biji jagung berpengaruh nyata terhadap semua parameter yaitu jumlah tunas, jumlah daun, dan tinggi tunas. Hasil terbaik rerata jumlah tunas pada perlakuan A4+B4 yaitu 5x10-6M NAA+10% ekstrak biji jagung. Hasil terbaik pada rerata jumlah daun pada perlakuan A2+B2 yaitu 5x10-7M NAA+5% ekstrak biji jagung dan hasil terbaik pada rerata tinggi tunas pada perlakuan A1+B1 yaitu 10-7M NAA+2,5% ekstrak biji jagung.Kata Kunci: multiplikasi; tunas anggrek hitam; ekstrak biji jagung; NAA. ABSTRACTGenerative reproduction of orchid plants it takes a requires the help of mycorriza mushrooms for seed germination, whereas conventional propagation business takes a long time to obtain large quantities of plants. One alternative to the propagation black orchids (Coelogyne pandurata Lindl.) is required through tissue culture techniques. The purpose of this study is to find the influence and concentration corn seed extract (Zea mays) and NAA (Naphthalene Acetic Acid) on the multiplication black orchids. This research was conducted in the tissue culture laboratory Biology Department Faculty of Mathematics and Natural Sciences Tanjungpura University Pontianak. The study used a Complete Randomized Design (RAL) of factorial patterns with two treatment factors. The first factor is that the NAA consists of 5 concentration levels  A0 (0 M) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) and A4 (5x10-6 M ) and the second factor is that corn seed extract of 5 levels concentratio B0(0%), B1 (2,5%), B2 (5%); B3 (7,5%) and B4 (10%). The administration NAA and corn seed extract in combination has a real effect on all parameters namely the number shoots, the number leaves, and the height shoots. The best results where the average number of shoots in the treatment of A2+B2 namely 5x10-6M NAA + 10% corn seed extract. The best results average number of leaves in the treatment  A2+B2 namely 5x10-7M NAA + 5% corn seed extract and in the best results for shoot height in the treatment of A1+B1 namely 10-7M NAA + 2.5% corn seed extract.Keywords: Multiplication; black orchid’s shoot; corn  seed extract; NAA

scholarly journals APLIKASI INDOLE-3-BUTYRIC ACID DAN NAPHTHALENE ACETIC ACID PADA BERBAGAI BAHAN SETEK JAMBU MADU DELI HIJAU DENGAN SISTEM HIDROPONIK NFT

2018 ◽  
Vol 14 (2) ◽  
pp. 92
Author(s):  
Rizka Novi Sesanti ◽  
Hilman Hidayat ◽  
Sismanto Sismanto
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Naphthalene Acetic Acid ◽  
Hydroponic System ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design

This research is about application IBA and NAA with some cutting materials of “Madu Deli Hijau” water apple using NFT hydroponic system was aim to study the growth of water apple’s cuttings was treated by IBA and NAA 500 ppm and study the best kind of cutting materials of water apple that can growth the most.  This research was conducted using completely randomized design in two factors.  The first was consisted of 7 treatments; control (without Auxin), IBA 500 ppm, and NAA 500 ppm. The second was consisted of 4 treatments; woody branch (hardwood), semi woody (semi hardwood), and soft branch, softwood, and shoots. The results showed that the added auxin in the form of IBA and NAA 500 ppm can increase the success of madu deli hijau water apple cuttings and the cuttings from the shoots are the best for the success of madu deli hijau water apple in NFT hydroponic system.

PENGARUH ZAT PENGATUR TUMBUH AUKSIN: INDOLE BUTIRIC ACID (IBA) DAN SITOKININ: BENZIL AMINO PURINE (BAP) dan KINETIN DALAM ELONGASI PERTUNASAN GAHARU (Aquilaria beccariana)

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Daru Mulyono
Keyword(s):  
Factorial Design ◽  
Randomized Design ◽  
Mother Plants ◽  
Effect Of Treatment ◽  
Optimal Formula

The objective of the research is to know the optimal formula of auksine and sitokinine in the Murashige dan Skoog (MS) basic media and to conserve gaharu tree (Aquilaria beccariana) in order to produce young gaharu plants which have similar properties with their mother plants. The research used Factorial Design with basic analysis of Complete Randomized Design in order to know the effect of treatment to the elongation. The results of the research showed that after 8 weeks of the treatment with IBA and the combination with BAP and Kinetin have significant effect to the increase of height and number of segment but have no significant effect to the number of buds. The combination of 0.1 mg/l IBA and BAP 0.05 mg/l was the optimal formula for the elongation of young gaharu plants with the increse of height 1.7 cm and number of segmnent 6.4.

scholarly journals Rooting Osha Crown Cuttings Using IBA and NAA

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1120A-1120
Author(s):  
Bennett J. Sondeno ◽  
Karen L. Panter
Keyword(s):  
Acetic Acid ◽  
Root Formation ◽  
Rocky Mountain ◽  
Deionized Water ◽  
Naphthalene Acetic Acid ◽  
Natural Light ◽  
Randomized Design ◽  
Ligusticum Porteri ◽  
Root Analysis ◽  
Completely Randomized Design

Osha (Ligusticum porteri) is a Rocky Mountain native frequently used as a medicinal herb. It is currently harvested largely from the wild. Studies have been under way since 2001 to find ways to propagate and produce the plant. To potentially increase rooting success of crown cuttings of osha, two different rooting hormones were used, each at two concentrations. Treatments were controls, 2500 ppm, and 5000 ppm solutions each of indole-3-butyric acid (IBA) and α-naphthalene acetic acid (NAA). Cuttings were soaked in deionized water or treatment solutions for 2 min. After soaking cuttings were stuck in sterilized sand in 725-mL2 containers, one cutting per container. Containers were placed on a mist propagation bench at 21 °C in a completely randomized design under natural light and day lengths. Data taken were days to visible root and shoot, and presence or absence of root formation after 50 days. Results indicated only one of 70 cuttings (<1%) produced a shoot. Roots formed on 14% of control cuttings, 64% in 2500 ppm IBA, 86% in 5000 ppm IBA, 36% in 2500 ppm NAA, and 14% in 5000 ppm NAA. Days to rooting ranged from 14.9 (2500 ppm IBA) to 29.0 (5000 ppm NAA). Due to considerable variation in days to rooting, and the number of cuttings that did not root, analysis of variance showed no differences among treatments. Frequency analysis indicated differences among treatments in root presence or absence. The 2500 and 5000 ppm IBA treatments showed more root formation than the controls or either NAA treatment. This indicates IBA may enhance rooting of osha crown cuttings.

scholarly journals Physiological potential of piquin pepper seeds in response to pregermination treatments

2021 ◽  
Vol 45 ◽  
Author(s):  
Miguel Ángel Cano-González ◽  
Benjamín Abraham Ayil-Gutiérrez ◽  
Rafael Delgado-Martínez ◽  
Eduardo Osorio-Hernández ◽  
José Antonio Rangel-Lucio ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salicylic Acid ◽  
Growth Regulators ◽  
Germination Percentage ◽  
Wild Plant ◽  
Naphthalene Acetic Acid ◽  
Distilled Water ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Pharmaceutical Properties

ABSTRACT Wild piquin pepper fruits are consumed and traded because of their flavor, nutritional and pharmaceutical properties. The heterogeneous germination of its seeds has caused pregermination treatments to be unstandardized. Because of that, our goal was to evaluate pregermination treatments of piquin pepper seeds from a wild plant from the municipality of Victoria, Tamaulipas, Mexico, consisting of immersion for different periods (2, 24, 48 h) in solutions with variable concentrations of the growth regulators naphthalene acetic acid (NAA; 0.1, 1.0, 2.5 mM), salicylic acid (SA; 1.0, 5.0, 10 mM), gibberellic acid (GA3, 1.0, 5.0, 10 mM), indole-3-butyric acid (IBA; 1.0, 2.0, 3.0 mM), the commercial product Biozyme (Bioz) containing minerals and growth regulators (Bioz; 0.5, 1, 2%), and sulfuric acid (H2SO4; 2, 5, 10%) in a completely randomized design, using distilled water as the control. For each treatment, we measured the imbibition ratio, germination percentage, time to obtain 50% germination (T50), and coefficient of variation of germination time (CVt). Our results showed that the imbibition was similar in the control and the treatments, but it differed between treatments. The highest imbibition recorded in 48 h was 87%. The highest germination percentage (79.2%) was achieved with 2 h immersion in 2% Bioz, followed by 1 mM NAA (62%) and 5 mM SA (56%). T50 and CVt were 10-26 d and 0-39%, respectively. According to our results, we concluded that the pregermination treatments we assayed improved the physiological potential for the germination of piquin pepper seeds.

scholarly journals The effect of naftalene acetic acid (NAA) on growth and essential oil contents of jasmine callus (Jasminum sambac (L.) Ait.)

2006 ◽  
Vol 4 (2) ◽  
pp. 41-44 ◽  
Author(s):  
GENINGSIH WIDYAWATI ◽  
SOLICHATUN SOLICHATUN ◽  
SOERYA DEWI MARLIYANA
Keyword(s):  
Gas Chromatography ◽  
Acetic Acid ◽  
Essential Oil ◽  
Significant Influence ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Jasminum Sambac

The objectives of the research was to determine the effect of naphthalene acetic acid (NAA) in variation concentration on the growth and the essential oil contents in Jasminum sambac (L.) Ait. callus. The research used a completely randomized design with one treatment due to NAA concentration, with five replications. The treatment was the application of NAA (0 mg/L; 0.5 mg/L; 1.0 mg/L; 1.5 mg/L; and 2.0 mg/L) on the MS media. The growth of J. sambac callus was examined after 5 weeks incubation. Essential oil contents of J. sambac callus were tested by Gas Chromatography (GC). The results of the research indicated that NAA did not give a significant influence on the growth and the essential oil contents of J. sambac callus.

scholarly journals RESPON PERTUMBUHAN ANGGREK TEBU (Grammatophyllum speciosum BLUME) SECARA IN VITRO TERHADAP PEMBERIAN BENZYL AMINO PURIN, KINETIN, NAFTALENA ACETIC ACID DAN EKSTRAK PISANG AMBON DALAM MEDIA DASAR SETENGAH MURASHIGE AND SKOOG

2021 ◽  
Vol 46 (1) ◽  
pp. 59
Author(s):  
Ellok Dwi Sulichantini ◽  
Eliyani Eliyani ◽  
Alvera Prihatini Dewinazari ◽  
Susylowati Susylowati ◽  
Agusty Saputra
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Leaf Length ◽  
Leaf Number ◽  
Leaf Width ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
The Media ◽  
Range Test

Kalimantan whose existence is increasingly extinct so it needs to be preserved by in vitro propagation. This study aims to determine the effect of a combination of the growth regulators, Benzyl Amino Purin (BAP), Kinetin, Naphthalene Acetic Acid (NAA) and banana extract on the growth of sugar cane orchids in vitro. The basic media used were Half Murashige and Skoog (1/2 MS), on the base media were added 30 g.L-1 sugar, 8 g.L-1 seaweed commercial powder,  plus BAP 3 ppm, Kinetin 3 ppm, NAA 0.5 ppm, and ambon banana extract 100 g.L-1, the acidity (pH) of the media is adjusted to around 5.8. The research design used was a Completely Randomized Design (CRD), each treatment was repeated six times. Data were analyzed using variance analysis and continued with DMRT (Duncan Multiple Range Test) tests with a level of 5%.            The results showed that the half media Murashige and Skoog was good for the propagation of sugarcane orchids.  The addition of BAP growth regulators, Kinetin, NAA, and banana extracts showed a good response to growth culture. The addition of BAP 3 ppm + NAA 0.5 ppm showed the best results for the height increased, leaf number increased, leaf length increased, leaf width increased, and the number of shots increased. The addition of Ambon banana extract to media containing growth regulators tended to decrease the growth response, seen in the parameters of plant height increased, leaf number increased, leaf length increased, leaf width increased, the number of shoots increased, the number of roots increased and in root length increased.

FTIR, GC-MS and HPLC analysis of Baliospermum montanum (Willd.) Muell. Arg.

2020 ◽  
Vol 11 (4) ◽  
pp. 5059-5066
Author(s):  
Sushma B K ◽  
Raveesha H R
Keyword(s):  
Acetic Acid ◽  
Quantitative Estimation ◽  
Chemical Constituents ◽  
Standard Procedure ◽  
Naphthalene Acetic Acid ◽  
Root Extract ◽  
Pentanoic Acid ◽  
Trimethylsilyl Ester ◽  
Isolation And Characterization ◽  
Ms Analysis

The present work is aimed to determine the chemical constituents in Baliospermum montanum methanolic extracts. An in vitro regenerated procedure was developed for the induction of callus from stem explant cultured on Murashige and Skoog (MS) medium fortified with various concentration and permutations of 2, 4-dichloro phenoxy acetic acid, 1-naphthalene acetic acid, 6-benzyl amino purine and gibberellic acid. FTIR &amp; GC-MS analysis was done according to standard procedure. The quantitative estimation of β-sitosterol was done by HPLC method. Maximum fresh and dry weight of callus was estimated in the combination of GA3 (0.5 mg/L) + NAA (2 mg/L) compared to other concentration. The FTIR analysis showed various functional compounds with different characteristic peak values in the extracts. Major bioactive constituents were recognized in the GC-MS analysis. Root extract revealed the existence of 1-hexadecanol, pentanoic acid, 2-(aminooxy)- and 1-hexacosanol. Leaf extract showed the presence of propanoic acid, 2-oxo-, trimethylsilyl ester, 9,12-octadecadienoic acid (z,z)-, trimethylsilyl ester, docosane, 1,22-dibromo- and pentatriacontane. Stem and stem derived callus exhibit the presence of 1,6,3,4-dihydro-2-deoxy-beta-d-lyxo-hexopyranose, n-hexadecanoic acid and pentanoic acid, 2-(aminooxy). The methanolic extract of leaf exhibited 0.2149 % of β-sitosterol content. There were no peaks observed in the root, stem and stem derived callus. Further studies are necessary for the isolation and characterization of bioactive compounds from B. montanum.

The effect of naphthalene acetic acid and some rooting media on rooting abilities and shoot growth of Dwarf Nerium cuttings

2017 ◽  
Vol 38 (5(SI)) ◽  
pp. 903-909
Author(s):  
H. Akat ◽  
◽  
G.C. Demirkan ◽  
A.L. Tuna ◽  
◽  
...  
Keyword(s):  
Acetic Acid ◽  
Shoot Growth ◽  
Naphthalene Acetic Acid ◽  
Rooting Media
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