Evaluation of Olives Cultivated in Southern Italy by Simple Sequence Repeat Markers

Olive (Olea europaea L.) is a species of great economic importance in the Mediterranean basin. Italy is very important for the olive industry; in fact, olive's genetic patrimony is very rich and characterized by an abundance of cultivars. At present, the majority of ancient landraces are vegetatively propagated by farm. It is likely that the number of cultivars is underestimated because of inadequate information on minor local cultivars that are widespread in different olive-growing areas. The existence of many cultivars reinforces the need for a reliable identification method. It is important to improve the ex situ plant germplasm collection and fairly to characterize all cultivars for future breeding programs. In the present report, we used 11 loci microsatellites to characterize 211 olive cultivars of an olive collection cultivated in six regions of southern Italy. These regions represent the major area for olive cultivation in Italy and have a strategic geographical location in the Mediterranean basin. The dendrogram obtained, using the unweighted pair group method with arithmetic mean clustering algorithm, depicts the pattern of relationships between the studied cultivars. There is a clear structuring of the variability relative to the geographic origin of olive cultivars. This work, for the very high number of the Italian olive cultivars analyzed, highlights the degree and distribution of genetic diversity of this species for better exploitation of olive resources and for the design of plant breeding programs. Besides, the use of molecular markers, like simple sequence repeats, is imperative to build a database for cultivar analysis, for traceability of processed food, and for appropriate management of olive germplasm collections.