scholarly journals Leaf Blade versus Petiole Nutrient Tests as Predictors of Nitrogen, Phosphorus, and Potassium Status of ‘Pinot Noir’ Grapevines

HortScience ◽  
2017 ◽  
Vol 52 (1) ◽  
pp. 174-184 ◽  
Author(s):  
R. Paul Schreiner ◽  
Carolyn F. Scagel

Grape growers rely on tissue tests of leaf blades or petioles for routine monitoring of vine nutritional health and for diagnosing potential nutrient deficiency or toxicity. There has been a long-standing debate as to which tissue better reflects the nutrient status of vines. A comparison of leaf blade and petiole nutrient concentrations was carried out to investigate which tissue better relates to vine growth, yield, and must nutrient responses of ‘Pinot noir’ grapevines to varying levels of nitrogen (N), phosphorus (P), and potassium (K) supply using data from a pot-in-pot vineyard over 4 years. Leaf blades and petioles were collected at 50% bloom and 50% veraison in each year and N, P, and K concentrations were assessed as predictors of leaf area at veraison, pruning mass at dormancy, yield, and must nutrient concentrations at fruit maturity. Data from commercial ‘Pinot noir’ vineyards were also used to investigate the relationship between leaf blade and petiole N concentrations with must N levels. Results indicated that leaf blades were superior to petioles in predicting vine growth, yield, and must yeast assimilable nitrogen (YAN) responses across a wide range of vine N status at both sampling times. Leaf blade N was a better predictor than petiole N in predicting YAN using data sets from both the pot-in-pot vineyard and commercial vineyards. Relationships between leaf blade and petiole concentrations of P and K and vine response variables generally did not differ and both tissues appeared to be equally effective in predicting P and K effects on growth, yield, and must P or K levels. Although petiole P was slightly better than leaf blade P at bloom in predicting must P levels, and models including both leaf and petiole K simultaneously as predictors relied only on leaf K. For all three nutrients, sampling at bloom and veraison had a similar predictive strength for response variables. Based on these findings, we recommend using leaf blades as opposed to petioles for diagnosing the N, P, and K status of ‘Pinot noir’.

HortScience ◽  
2014 ◽  
Vol 49 (10) ◽  
pp. 1335-1340 ◽  
Author(s):  
R. Paul Schreiner ◽  
Jungmin Lee

‘Pinot noir’ grapevines were grown in a pot-in-pot system using a red-hill-soil where volumetric soil water content (θv) was carefully controlled. Four-year-old vines were supplied with one of two irrigation regimes (wet or dry) between véraison and fruit maturity and the experiment was repeated over 2 years. From véraison to harvest, vines in the wet treatment received irrigation whenever θv approached ≈15% maintaining leaf water potential (Ψleaf) above –1.0 MPa. Vines in the dry treatment received irrigation when θv approached 11% to 12% and experienced significant water stress (Ψleaf ≈–1.4 MPa) before water was re-supplied. Vines were destructively harvested at véraison and at fruit maturity to determine biomass and nutrient content in the current season’s above-ground tissues. Fruit yield, maturity indices, and must nutrient composition were measured at maturity. Irrigation did not influence vine growth in either year nor did it influence yield or fruit maturity indices. Irrigation also had no influence on leaf, whole cluster, or must mineral nutrient concentrations. Vine growth, yield, and nutrient status in leaves and musts varied by year. Vegetative growth was greater in 2007 than 2008, whereas yield and cluster weights were greater in 2008. Also in 2008, whole clusters obtained a greater proportion of dry matter and nutrients after véraison when differing irrigation treatments were imposed. Nonetheless, irrigation did not affect must chemical composition. These findings suggest that periodic post-véraison water deficits that are moderate to severe have little effect on berry nutrient and sugar accumulation in ‘Pinot noir’ cropped at typical levels for this variety.


2016 ◽  
Vol 26 (1) ◽  
pp. 36-45 ◽  
Author(s):  
Lindsay M. Jordan ◽  
Thomas Björkman ◽  
Justine E. Vanden Heuvel

In the cool and humid climate of the northeastern United States, vegetation is typically maintained between the rows of wine grape (Vitis vinifera) vineyards, but the area directly beneath vines is conventionally kept bare using herbicides or cultivation, to reduce competition for water and nutrients. Yet with rising concerns of herbicide resistance, environmental contamination, and soil erosion, alternatives to maintaining bare ground in vineyards should be considered. In warmer and more arid climates, using cover crops as an alternative to bare soil has sometimes resulted in reduced vine growth and yields. In more cool and humid climates, like in the northeastern United States, where conditions can promote excessive vine growth, replacing bare soil with under-vine cover crops was hypothesized to improve vine growth characteristics and fruit quality from reducing excessive vigor. This study compared three annual under-vine cover crops of resident vegetation (RES), buckwheat (BW) (Fagopyrum esculentum), and annual ryegrass (ARG) (Lolium multiflorum), planted in the 1-m-wide strip directly under vines at the start of each growing season, against the conventional weed-free under-vine row maintained with glyphosate. The experiment was established in 2011 and repeated in 2012 and 2013 in a 20-year-old block of ‘Riesling’ wine grapes (clone 198 on S04 rootstock) in a commercial vineyard in the Finger Lakes region of New York State. Harvested grapes were fermented in duplicate using standard white wine procedures. Among the four under-vine treatments, no significant differences were found in measures of vegetative growth, yield, petiole nutrient concentrations at veraison, or predawn and midday stem water potentials. Under-vine treatments were not found to significantly affect soil organic matter, aggregate stability, and nutrient concentrations. Juice characteristics were also not significantly different among treatments. In this study, the mature vines in this rain-fed ‘Riesling’ vineyard likely had a well-developed and extensive rooting system that was able to overcome any competition effects for water or nutrients from the comparatively shallow root systems of the annually established cover crops. Without any induced competition in the conditions of this study, under-vine cover crops had no effects on vine growth, yield, or juice characteristics when compared with conventional herbicide use in the under-vine row. When multidimensional scaling (MDS) analysis was used to determine differences in aroma among wine treatment replicates, treatments were found to significantly impact the perceived aromatic properties of the wines, even though no measures of growth or juice characteristics were affected. Using under-vine vegetation may be a viable alternative to conventional herbicide use for vineyard floor management in mature wine grape vineyards in cool and humid climates.


Author(s):  
Gunta Čekstere ◽  
Anita Osvalde ◽  
Māris Laiviņš

Abstract The research objectives were: 1) to determine healthy young ash supply with nutrients and their concentrations in natural growth conditions; 2) to estimate nutrient balance and inter-correlations within a system “soil-plants”; and 3) to determine the potential effect of environmental conditions (forest type, plant phytosociological group, soil group, etc.) on nutrient accumulation in soil and leaves of young ash. The investigation was conducted in 28 different forest sites with young ash in Latvia. The results demonstrated that young ash grows well on a wide range of site types in terms of soil composition, forest type and phytosociological tree group. Although highly heterogeneous, soil in ash stands in Latvia can provide a sufficient supply of nutrients. Therefore, the nutrient status of healthy young ash leaves can be characterized as sufficient, although low levels of P, Zn, and K in leaves was found for most sites. Significant impact of soil group and forest type was found on nutrient composition of ash soil, while leaf nutrient concentrations were more dependent on the forest type and phytosociological tree group. The obtained results confirmed the ability of ash to accumulate nutrients within a certain range from soils of different fertility, organic matter content and soil reaction in Latvia.


2012 ◽  
Vol 39 (12) ◽  
pp. 1038 ◽  
Author(s):  
Ruth Reef ◽  
Nele Schmitz ◽  
Britt A. Rogers ◽  
Marilyn C. Ball ◽  
Catherine E. Lovelock

Salinisation of the soil can cause plant water deficits, ion and nutrient imbalances and toxic reactions. The halophyte, Avicennia marina (Forssk.) Vierh., is a mangrove that tolerates a wide range of soil salinities. In order to understand how salinity affects plant growth and functioning and how salinity responses are influenced by the water deficit signalling hormone abscisic acid (ABA) we grew A. marina seedlings under two non-growth limiting salinities: 60% seawater and 90% seawater and with and without exogenously supplied ABA. We measured growth, photosynthesis, sap flow, aquaporin gene expression, hydraulic anatomy and nutrient status as well as sap ABA concentrations. ABA addition resulted in a drought phenotype (reduced sap flow, transpiration rates and photosynthesis and increased water use efficiency and aquaporin expression). In contrast, growth in high salinity did not lead to responses that are typical for water deficits, but rather, could be characterised as drought avoidance strategies (no reduction in sap flow, transpiration rates and photosynthesis and reduced aquaporin expression). Tissue nutrient concentrations were higher in seedlings grown at high salinities. We did not find evidence for a role for ABA in the mangrove salinity response, suggesting ABA is not produced directly in response to high concentrations of NaCl ions.


Author(s):  
Agustina Sánchez-Parada ◽  
Miguel Ángel López-López ◽  
Armando Gómez-Guerrero ◽  
Marlín Pérez-Suárez

Pinus patula is one of the most planted wood conifer species worldwide; however, no foliar nutrient standards exist for this species up to date. The objective of the present study was to generate and verify two sets of foliar nutrient standards for nearly ten-year-old P. patula trees: critical nutrient concentrations and DRIS norms. Nutrients studied were N, P, K, Ca, Mg, Fe, Cu, Zn, Mn, and B. The reference standards were verified experimentally by installing two fertilization trials; one of them located in Huayacocotla, state of Veracruz and the other one in Aquixtla, state of Puebla, Mexico. Nutrient status of each fertilization trial was correctly predicted by critical nutrient values and DRIS as well. Both standards were able to detect the secondary growth-limiting nutrient deficiency in the Huayacocotla trial, where the primary limitation for growth was scarcity of solar radiation within tree crowns. The limiting nutrient in both experimental trials was K.


Biology ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 426
Author(s):  
Kimberly Sánchez-Alonzo ◽  
Fabiola Silva-Mieres ◽  
Luciano Arellano-Arriagada ◽  
Cristian Parra-Sepúlveda ◽  
Humberto Bernasconi ◽  
...  

Helicobacter pylori, a Gram-negative bacterium, has as a natural niche the human gastric epithelium. This pathogen has been reported to enter into Candida yeast cells; however, factors triggering this endosymbiotic relationship remain unknown. The aim of this work was to evaluate in vitro if variations in nutrient concentration in the cultured medium trigger the internalization of H. pylori within Candida cells. We used H. pylori–Candida co-cultures in Brucella broth supplemented with 1%, 5% or 20% fetal bovine serum or in saline solution. Intra-yeast bacteria-like bodies (BLBs) were observed using optical microscopy, while intra-yeast BLBs were identified as H. pylori using FISH and PCR techniques. Intra-yeast H. pylori (BLBs) viability was confirmed using the LIVE/DEAD BacLight Bacterial Viability kit. Intra-yeast H. pylori was present in all combinations of bacteria–yeast strains co-cultured. However, the percentages of yeast cells harboring bacteria (Y-BLBs) varied according to nutrient concentrations and also were strain-dependent. In conclusion, reduced nutrients stresses H. pylori, promoting its entry into Candida cells. The starvation of both H. pylori and Candida strains reduced the percentages of Y-BLBs, suggesting that starving yeast cells may be less capable of harboring stressed H. pylori cells. Moreover, the endosymbiotic relationship between H. pylori and Candida is dependent on the strains co-cultured.


2021 ◽  
pp. 1-17
Author(s):  
Anna Assimakopoulou ◽  
Ioannis Salmas ◽  
Aikaterina Tsikra ◽  
Alexandros-Iasonas Bastas ◽  
Maria Bakea ◽  
...  

BMJ Open ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. e047007
Author(s):  
Mari Terada ◽  
Hiroshi Ohtsu ◽  
Sho Saito ◽  
Kayoko Hayakawa ◽  
Shinya Tsuzuki ◽  
...  

ObjectivesTo investigate the risk factors contributing to severity on admission. Additionally, risk factors of worst severity and fatality were studied. Moreover, factors were compared based on three points: early severity, worst severity and fatality.DesignAn observational cohort study using data entered in a Japan nationwide COVID-19 inpatient registry, COVIREGI-JP.SettingAs of 28 September 2020, 10480 cases from 802 facilities have been registered. Participating facilities cover a wide range of hospitals where patients with COVID-19 are admitted in Japan.ParticipantsParticipants who had a positive test result on any applicable SARS-CoV-2 diagnostic tests were admitted to participating healthcare facilities. A total of 3829 cases were identified from 16 January to 31 May 2020, of which 3376 cases were included in this study.Primary and secondary outcome measuresPrimary outcome was severe or nonsevere on admission, determined by the requirement of mechanical ventilation or oxygen therapy, SpO2 or respiratory rate. Secondary outcome was the worst severity during hospitalisation, judged by the requirement of oxygen and/orinvasive mechanical ventilation/extracorporeal membrane oxygenation.ResultsRisk factors for severity on admission were older age, men, cardiovascular disease, chronic respiratory disease, diabetes, obesity and hypertension. Cerebrovascular disease, liver disease, renal disease or dialysis, solid tumour and hyperlipidaemia did not influence severity on admission; however, it influenced worst severity. Fatality rates for obesity, hypertension and hyperlipidaemia were relatively lower.ConclusionsThis study segregated the comorbidities influencing severity and death. It is possible that risk factors for severity on admission, worst severity and fatality are not consistent and may be propelled by different factors. Specifically, while hypertension, hyperlipidaemia and obesity had major effect on worst severity, their impact was mild on fatality in the Japanese population. Some studies contradict our results; therefore, detailed analyses, considering in-hospital treatments, are needed for validation.Trial registration numberUMIN000039873. https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000045453


2015 ◽  
Vol 47 (2) ◽  
pp. 169-206 ◽  
Author(s):  
Andrew S. Fullerton ◽  
Jun Xu

Adjacent category logit models are ordered regression models that focus on comparisons of adjacent categories. These models are particularly useful for ordinal response variables with categories that are of substantive interest. In this article, we consider unconstrained and constrained versions of the partial adjacent category logit model, which is an extension of the traditional model that relaxes the proportional odds assumption for a subset of independent variables. In the unconstrained partial model, the variables without proportional odds have coefficients that freely vary across cutpoint equations, whereas in the constrained partial model two or more of these variables have coefficients that vary by common factors. We improve upon an earlier formulation of the constrained partial adjacent category model by introducing a new estimation method and conceptual justification for the model. Additionally, we discuss the connections between partial adjacent category models and other models within the adjacent approach, including stereotype logit and multinomial logit. We show that the constrained and unconstrained partial models differ only in terms of the number of dimensions required to describe the effects of variables with nonproportional odds. Finally, we illustrate the partial adjacent category logit models with empirical examples using data from the international social survey program and the general social survey.


Sign in / Sign up

Export Citation Format

Share Document