scholarly journals Reexamining the Classification of Theobroma cacao L. Using Molecular Markers

1994 ◽  
Vol 119 (5) ◽  
pp. 1073-1082 ◽  
Author(s):  
Antonio Figueira ◽  
Jules Janick ◽  
Morris Levy ◽  
Peter Goldsbrough
Keyword(s):  
Molecular Markers ◽  
Theobroma Cacao ◽  
Rapd Markers ◽  
Continuous Variation ◽  
Clear Distinction ◽  
Species Separation ◽  
Theobroma Cacao L ◽  
Rdna Polymorphism

Genetic similarities among eight Theobroma and two Herrania species, including 29 genotypes of T. cacao, were estimated by rDNA polymorphism. A phenogram based on these genetic similarities significantly separated two clusters: one cluster included all Herrania and Theobroma species, except T. cacao, while the second contained 28 of 29 T. cacao genotypes. There was no clear distinction between Herrania and Theobroma species. Separation of 29 T. cacao genotypes, representing all races and various origins, had no congruency with the conventional classification into three horticultural races: Criollo, Forastero, and Trinitario. Genetic similarities in T. cacao, estimated with RAPD markers, indicated continuous variation among the generally similar but heterogeneous genotypes. The wild genotypes formed an outgroup distinct from the cultivated genotypes, a distinction supported by the rDNA data. The phenograms constructed from RAPD and rDNA data were not similar within the wild and cultivated cacao subsets.

scholarly journals Inheritance of Random Amplified Polymorphic DNA (RAPD) Markers in Theobroma cacao L.

1995 ◽  
Vol 120 (4) ◽  
pp. 681-686 ◽  
Author(s):  
C.M. Ronning ◽  
R.J. Schnell ◽  
D.N. Kuhn
Keyword(s):  
Genetic Analysis ◽  
Theobroma Cacao ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Polymorphic Locus ◽  
Random Amplified Polymorphic Dna ◽  
Phenotypic Expression ◽  
Backcross Population ◽  
Backcross Family ◽  
Theobroma Cacao L

RAPD markers have been used successfully in genetic analysis of several crop plants. This method poses difficulties with a highly heterozygous species such as Theobroma cacao because of the dominant phenotypic expression of bands. A backcross family derived from ctultivars Catongo and Pound 12 was analyzed to determine the efficacy of RAPD markers in analyzing cacao populations. A preliminary screen of the parents and the F1 plant used as the backcross parent was conducted with 180 RAPD primers; of these, 26% were polymorphic and reproducible and produced 104 storable loci. Genomic DNA from 54 individuals of the backcross population was then amplified with these primers; 68.3 % of the loci segregated as expected in a Mendelian fashion. Separation of RAPD fragments on acrylamide revealed an additional polymorphic locus from one primer that was indistinguishable on agarose. The results demonstrated that RAPD markers can be used to study the cacao genome.

scholarly journals Determination of Genetic Stability in Cacao Plants (Theobroma Cacao L.) Derived from Somatic Embryogenesis Using Microsatellite Molecular Markers (SSR)

2021 ◽  
Vol 21 (1) ◽  
pp. 284-298
Author(s):  
Ana María Henao-Ramírez ◽  
Héctor Jaime Salazar Duque ◽  
Arley Fernando Calle Tobón ◽  
Aura Inés Urrea Trujillo
Keyword(s):  
Somatic Embryogenesis ◽  
Molecular Markers ◽  
Genetic Stability ◽  
Theobroma Cacao ◽  
Theobroma Cacao L

Classification of cacao beans (Theobroma cacao L.) of southern Mexico based on chemometric analysis with multivariate approach

2015 ◽  
Vol 240 (6) ◽  
pp. 1117-1128 ◽  
Author(s):  
Alfredo Vázquez-Ovando ◽  
Francisco Molina-Freaner ◽  
Juan Nuñez-Farfán ◽  
David Betancur-Ancona ◽  
Miguel Salvador-Figueroa
Keyword(s):  
Theobroma Cacao ◽  
Chemometric Analysis ◽  
Multivariate Approach ◽  
Southern Mexico ◽  
Theobroma Cacao L

scholarly journals Inheritance of lethal gene ‘luteus-Pa’ from cacao progenies obtained by self and cross-fertilization

2017 ◽  
Vol 70 (2) ◽  
pp. 8123-8128
Author(s):  
Zurel N Meza S ◽  
Luis Fernando García Carrión ◽  
Patricia Vanessa García Rodríguez
Keyword(s):  
Molecular Markers ◽  
Functional Genomics ◽  
Theobroma Cacao ◽  
Genetic Material ◽  
Lethal Gene ◽  
Chi Square ◽  
Mendelian Segregation ◽  
Monogenic Inheritance ◽  
Theobroma Cacao L ◽  
Cross Fertilization

With the aim to study the inheritance of lethal gene ‘luteus-Pa’ in three derivated progenies from self and cross-fertilization of cacao (Theobroma cacao L) varieties, a study was carried out during January to September 2013. As genetic material C-25, S-5 y Pa-150 varieties were utilized and this one were self and cross pollinated and their progenies were divided in two replicates with 100 seeds each and sowed in plastic recipients containing a substrate 2:1(soil: sand). In order to contrast theobserved segregation with its expected homologous 3:1 (monogenic inheritance), the Chi-square (X2) test was utilized. The results shown that only one from self-fertilized progeny of C-25 variety exhibited the mendelian segregation 3:1 in both replicates, so that it lead to infer this one is carrier of lethal gene ‘luteus-Pa’ in heterozygote condition whereas that the progeny from Pa-150 x C-25 cross no found none lethal seedling and submitted to Chi-square (X2) test it could be to infer this one no segregate in the proportion 3:1 therefore, the Pa-150 variety is no carrier of this lethal gene. A better comprehension on photobiological origin, supported by molecular markers and the functional genomics of this mutant can help to identify and understand the factors involve in the photosynthetic mechanisms.

scholarly journals Comparative genetic diversity studies of Theobroma cacao L. using RFLP and RAPD markers

Heredity ◽  
1994 ◽  
Vol 73 (6) ◽  
pp. 589-597 ◽  
Author(s):  
J A K N'Goran ◽  
V Laurent ◽  
A M Risterucci ◽  
C Lanaud
Keyword(s):  
Genetic Diversity ◽  
Theobroma Cacao ◽  
Rapd Markers ◽  
Diversity Studies ◽  
Theobroma Cacao L

Situación económica y social de la población de San José del Carmen, San Cristóbal de las Casas, Chiapas

2019 ◽  
Vol 12 (4) ◽  
Author(s):  
Pedro Cadena-Iñiguez ◽  
Eileen Salinas-Cruz ◽  
Jesús Martínez-Sánchez ◽  
Mariano Morales-Guerra ◽  
Romualdo Vásquez-Ortiz ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Theobroma Cacao ◽  
Zea Mays L ◽  
Solanum Tuberosum L ◽  
San Jose ◽  
Phaseolus Vulgaris L ◽  
Las Casas ◽  
Vicia Faba L ◽  
Las Mujeres ◽  
Theobroma Cacao L

Objetivo: Establecer una línea base como fundamento para la intervención e inducción de innovaciones a través de escuelas de campo y planes de negocios. Diseño/metodología/aproximación: El estudio se desarrolló en San José del Carmen, San Cristóbal de Las Casas, Chiapas, México. Se elaboró una encuesta como instrumento para recabar en campo información de familias de la localidad con la finalidad de conocer su situación actual. La localidad de trabajo se eligió utilizando como criterio las poblaciones que se encuentran dentro de Cruzada Nacional contra el hambre y en la clasificación de la pobreza extrema Resultados: La localidad de San José del Carmen es considerada como de alta marginación, la población es bilingüe, su principal lengua es el Tzotzil, el nivel de estudios promedio es hasta el tercer año de primaria, las principales actividades productivas son la siembra de maíz (Zea mays L.), frijol (Phaseolus vulgaris L.), papa (Solanum tuberosum L.) y haba (Vicia faba L.) que son utilizadas principalmente para autoconsumo.  No cuentan con agua potable ya que su sistema es a través de la recaudación de agua de lluvia o a través de un jagüey. Sus principales actividades económicas son la albañilería por parte de los hombres, y venta de productos, tales como el pozol (bebida energética a base de maíz y cacao (Theobroma cacao L.), tostadas y algunos vegetales por parte de las mujeres. Las tostadas son producidas en forma artesanal con un proceso de doble nixtamalización y vendida en bolsas de 20 tostadas cada una, a un costo de MX$10.00 en los mercados de San Cristóbal de las Casas, una a dos veces por semana alrededor de 40 bolsas por día de venta. Limitaciones del estudio/implicaciones: La afiliación zapatista de alguno de los pobladores y el hecho de que algunos habitantes no hablen español, fueron factores que limitaron desarrollar al 100% el estudio; sin embargo, se considera que se tienen los elementos necesarios para establecer la línea base de la situación de San José del Carmen. Conclusiones: El diagnóstico servirá como una herramienta para establecer una intervención a través de innovaciones y planes de negocios en la comunidad de estudio. Los pobladores cuentan con herramientas necesarias para obtener nuevos conocimientos que ayuden a un desarrollo de su producción. El idioma no deberá de ser una limitante para ello.

CLASSIFICAÇÃO DE AMÊNDOAS E QUALIDADE DE LIQUOR DE CACAU (Theobroma cacao L.) SUPERIOR BAHIA

2013 ◽  
Vol 15 (4) ◽  
pp. 391-396 ◽  
Author(s):  
B.A. Melo Neto ◽  
E.A. Carvalho ◽  
D.L.N. Mello ◽  
A.C. Anjos ◽  
A.C.R. Ferreira ◽  
...  
Keyword(s):  
Theobroma Cacao ◽  
Theobroma Cacao L

Genetic Diversity of Basil (Ocimum spp. - Lamiaceae) Based on RAPD Markers and Volatile Oil Constituents

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 492f-493
Author(s):  
Roberto F. Vieira ◽  
James E. Simon ◽  
Peter Goldsbrough ◽  
Antonio Figueira
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Volatile Oil ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Traditional Medicines ◽  
The Tropics ◽  
High Degree ◽  
First Time

Essential oils extracted from basil (Ocimum spp.) by steam distillation are used to flavor foods, oral products, in fragrances, and in traditional medicines. The genus Ocimum contains around 30 species native to the tropics and subtropics, with some species naturalized and/or cultivated in temperate areas. Interand intraspecific hybridization have created significant confusion in the botanical systematics of this genus. Taxonomy of basil (O. basilicum) is also complicated by the existence of numerous varieties, cultivars, and chemotypes within the species that do not differ significantly in morphology. In this study we are using RAPD markers and volatile oil composition to characterize the genetic diversity among the most economically important Ocimum species. We hypothesize that the genetic similarity revealed by molecular markers will more accurately reflect the morphological and chemical differences in Ocimum than essential oil composition per se. Preliminary research using five Ocimum species, four undetermined species, and eight varieties of O. basilicum (a total of 19 accessions) generated 107 polymorphic fragments amplified with 19 primers. RAPDs are able to discriminate between Ocimum species, but show a high degree of similarity between O. basilicum varieties. The genetic distance between nine species and among 55 accessions within the species O. americanum, O. basilicum, O. campechianum, O. × citriodorum, O. gratissimum, O. kilimandscharium, O. minimum, O. selloi, and O. tenuiflorum will be analyzed by matrix of similarity and compared to the volatile oil profile. This research will for the first time apply molecular markers to characterize the genetic diversity of Ocimum associate with volatile oil constituent.

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