scholarly journals Mapping of the Egusi Seed Trait Locus (eg) and Quantitative Trait Loci Associated with Seed Oil Percentage in Watermelon

2012 ◽  
Vol 137 (5) ◽  
pp. 311-315 ◽  
Author(s):  
Jason Prothro ◽  
Katherine Sandlin ◽  
Rattandeep Gill ◽  
Eleni Bachlava ◽  
Victoria White ◽  
...  

The egusi watermelon (Citrullus lanatus) is popular in West Africa for its oil and protein-rich seed, which is consumed in soups and stews. The egusi phenotypic trait is controlled by a single recessive gene (eg) and is characterized by large seed size and fleshy, thick pericarp. An F2 mapping population was derived from Strain II (PI 279461) of the Japanese cultivar Yamato-cream with normal seed type and low seed oil percentage (SOP = 25.2%) and an egusi type from Nigeria [Egusi (PI 560023)] with high SOP (40.6%). Genetic analysis confirmed that the egusi seed trait is controlled by a single recessive gene (eg) and the location of the gene was mapped to 57.8 cM on linkage group (LG) 2, between markers NW0248325 and NW0250248. Four main quantitative trait loci (M-QTL) were identified for SOP in the population with the eg locus contributing 84% of the explained phenotypic variation (R2). A significant epistatic interaction (E-QTL) was identified between, the eg locus and an M-QTL on LG 9B. The present study reports the location of the eg locus responsible for the egusi seed trait in watermelon on LG 2 as well as M-QTL and E-QTL associated with SOP.

2016 ◽  
Vol 96 (3) ◽  
pp. 423-432 ◽  
Author(s):  
Xiaomao Cheng ◽  
Shu Xia ◽  
Xihua Zeng ◽  
Jianxun Gu ◽  
Yuan Yang ◽  
...  

Seed oil content is a key seed quality trait determining the economic value of rapeseed (Brassica napus L.). However, it is a complex quantitative trait controlled by multiple genes. To this point, its genetic mechanism in rapeseed remains to be revealed. In the present study, we separately identified the quantitative trait loci (QTL) controlling seed oil content of B. napus using three generations of recombinant inbred line (RIL) populations (F4:5, F5:6, and F6:7) derived from a cross of two contrasting parents (M201, a high-oil parent, and M202, a low-oil parent) in four trials. The results indicated that the additive effects may be the primary factors contributing to the variation in seed oil content in B. napus. A total of 15 QTL for seed oil content were mapped. Two of them, namely qOC-A9-3 and qOC-A10, were consistently detected across two and all four environments, respectively. Meanwhile, qOC-A10 showed a large effect on phenotypic variation in seed oil content. The stability and significance of qOC-A10 was also validated in the near isogenic lines (NILs-qOC-A10) developed from the RIL population (F4:5) using marker-assisted selection. The qOC-A10 is of particular interest for further fine mapping and map-based cloning.


2007 ◽  
Vol 2007 ◽  
pp. 6-6
Author(s):  
H.D. Daetwyler ◽  
F.S. Schenkel ◽  
M. Sargolzaei ◽  
J.A.B. Robinson

Quantitative trait loci (QTL) are chromosome regions which are significantly associated with the expression of a phenotypic trait in a particular population. Detection of a QTL is carried out using association with a genetic marker, such as a single nucleotide polymorphism (SNP), which is in linkage disequilibrium (LD) with the QTL. The two main categories of association studies are linkage analyses (LA), which consider LD within families and linkage disequilibrium methods, which make use of LD across an entire population. The recent reduction in genotyping costs has allowed for testing individuals for a large number of SNP. This substantial increase in genotypic data has lead to denser marker distributions on the bovine genome thus potentially increasing the power of QTL detection studies. The objective of this study was to scan the bovine genome to detect QTL for 305 day lactation milk yield (MY), 305 day lactation fat yield (FY), 305 day lactation protein yield (PY), herd life (HL), somatic cell score (SCS), interval from calving to first service in cows (CTFS) and age at first service in heifers (AFS). HL is a measure of longevity measured in the number of lactations a cow stays in the herd. SCS refers to the amount of somatic cells a cow has in her milk and is an important indicator trait for mastitis. CTFS is the period from parturition to first insemination in days and AFS is the age in days at which a heifer was artificially inseminated for the first time. Fertility traits, such as CTFS and AFS, are indicators of reproductive efficiency.


2008 ◽  
Vol 21 (2) ◽  
pp. 198-207 ◽  
Author(s):  
Ophélie Sicard ◽  
Olivier Loudet ◽  
Joost J. B. Keurentjes ◽  
Thierry Candresse ◽  
Olivier Le Gall ◽  
...  

In compatible interactions between plants and viruses that result in systemic infection, symptom development is a major phenotypic trait. However, host determinants governing this trait are mostly unknown, and the mechanisms underlying it are still poorly understood. In a previous study on the Arabidopsis thaliana–Plum pox virus (PPV) pathosystem, we showed a large degree of variation in symptom development among susceptible accessions. In particular, Cvi-1 (Cape Verde islands) accumulates viral particules but remains symptomless, Col-0 (Columbia) sometimes shows weak symptoms compared with Ler (Landsberg erecta), which always shows severe symptoms. Genetic analyses of Col × Ler and Cvi × Ler F2 and recombinant inbred line (RIL) populations suggested that symptom development as well as viral accumulation traits are polygenic and quantitative. Three of the symptom quantitative trait loci (QTL) identified could be confirmed in near-isogenic lines, including PSI1 (PPV symptom induction 1), which was identified on the distal part of chromosome 1 in both RIL populations. With respect to viral accumulation, several factors have been detected and, interestingly, in the Col × Ler population, two out of three viral accumulation QTL colocalized with loci controlling symptom development, although correlation analysis showed weak linearity between symptom severity and virus accumulation. In addition, in the Cvi × Ler RIL population, a digenic recessive determinant controlling PPV infection was identified.


Author(s):  
Priyono Priyono ◽  
Ucu Sumirat ◽  
Crouzillat Crouzillat

Recently the use of molecular markers has been successfully applied for some crops. For coffee, new opportunities have been opened since Nestlé R&D Centre in collaboration with ICCRI completed the first genetic map of Coffea canephora. This study was aimed both to evaluate the phenotypic trait and also to identify the quantitative trait loci (QTLs) controlling the vegetative growth in Robusta coffee. Present study used three C. canephora populations and six genetic maps developed based on these populations using simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs) markers. A total of 17 different quantitative data were used for the detection of QTLs on each of three populations. Present result showed that most of these traits were not heritable. The nine vegetative traits have been identified and distributed over seven different linkage groups. Due to some QTLs determining one given trait were overlapping on the same linkage group and were coming from the same favourable parent, a total of 19 QTLs detected for vegetative traits might finally be considered as only 12 QTLs involved. However, only two of them were shared for different traits. One involved for the number/length of primary branches and width of the canopy while the other for length of internodes and width of canopy. These two QTLs might determine the size of the tree canopy in this species. Key words: Coffea canephora, heridity, quantitative trait loci (QTLs), vegetative growth.


2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Yanjie Yao ◽  
Qingbo You ◽  
Guozhan Duan ◽  
Jianjun Ren ◽  
Shanshan Chu ◽  
...  

Euphytica ◽  
2014 ◽  
Vol 202 (3) ◽  
pp. 411-426 ◽  
Author(s):  
Shi Liu ◽  
Peng Gao ◽  
Xuezheng Wang ◽  
Angela R. Davis ◽  
Abdul Majeed Baloch ◽  
...  

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