Rapid detection of Listeria monocytogenes in meat products by loop-mediated isothermal amplification (LAMP) – bioluminescent assay

Vsyo o myase ◽  
2019 ◽  
pp. 40-41
Author(s):  
T. Mori ◽  
◽  
K. Kishino ◽  
S. Wada ◽  
T. Nanba ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Rapid Detection ◽  
Meat Products ◽  
Isothermal Amplification ◽  
Bioluminescent Assay ◽  
Loop Mediated Isothermal Amplification

Reverse transcription – loop-mediated isothermal amplification assay for the rapid detection of pathogenic Listeria monocytogenes in meat products

2019 ◽  
Vol 65 (12) ◽  
pp. 913-921
Author(s):  
Ling-Zhi Zhan ◽  
Da-Feng Song ◽  
Qing Gu ◽  
Ting-Ting Yan ◽  
Cong-Cong Ma
Keyword(s):  
Listeria Monocytogenes ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Meat Products ◽  
Isothermal Amplification ◽  
National Standard ◽  
Raw Meat ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay ◽  
Meat Samples

This study reports the use of reverse transcription – loop-mediated isothermal amplification (RT–LAMP) to detect Listeria monocytogenes in meat. The assay was designed to target the iap gene of L. monocytogenes, to which four primers, recognizing six distinct iap sites, were designed. We optimized the RT–LAMP conditions and established the following optimal systems: 60 min, 63 °C, 2.0 mmol/L MgSO4, 1.0 mol/L betaine, 2.0 mmol/L dNTPs, 320 U/mL Bst DNA polymerase, 0.4 μmol/L outer primers, and 0.8 μmol/L inner primers. The RT–LAMP amplification products were identified by a visible white Mg2P2O7 precipitate or electrophoresis on a 2% agarose gel. RT–LAMP has a sensitivity of 7.3 × 101 CFU/mL, which is 2-fold higher than that of LAMP. When commercially available raw meat samples (including beef, pork, mutton, and rabbit) were analyzed simultaneously with RT–LAMP and the Chinese National Standard GB 4789.30-2016, their abilities to detect L. monocytogenes were the same. Samples containing L. monocytogenes killed by 15 psi at 121 °C for 15 min were used to confirm the specificity of RT–LAMP for live microorganisms. Thus, we used RT–LAMP to efficiently detect L. monocytogenes in meat products.

Rapid Detection of Listeria monocytogenes in Raw Milk with Loop-Mediated Isothermal Amplification and Chemosensor

2011 ◽  
Vol 76 (9) ◽  
pp. M611-M615 ◽  
Author(s):  
Deguo Wang ◽  
Gaiping Zhang ◽  
Chengping Lu ◽  
Ruiguang Deng ◽  
Aimin Zhi ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Rapid Detection ◽  
Raw Milk ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

A LOOP-MEDIATED ISOTHERMAL AMPLIFICATION ASSAY FOR RAPID DETECTION OF LISTERIA MONOCYTOGENES

2011 ◽  
Vol 31 (4) ◽  
pp. 546-552 ◽  
Author(s):  
XIAO-LI WANG ◽  
FENG-ZHEN GENG ◽  
XIAN-ZHOU ZHANG ◽  
YU WANG ◽  
XIAO-YAN MA ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Rapid Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay

Rapid detection of food-borne Listeria monocytogenes by real-time quantitative loop-mediated isothermal amplification

2012 ◽  
Vol 21 (1) ◽  
pp. 101-106 ◽  
Author(s):  
Xiaoxiao Shan ◽  
Yaoqi Zhang ◽  
Zhigang Zhang ◽  
Miaorui Chen ◽  
Yongyu Su ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Real Time ◽  
Rapid Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Food Borne
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