Late Fall Site‐Specific Soil Nitrate Upper Limits for Groundwater Protection Purposes

1995 ◽  
Vol 24 (4) ◽  
pp. 725-733 ◽  
Author(s):  
R. R. Van Der Ploeg ◽  
H. Ringe ◽  
Galina Machulla
1970 ◽  
Vol 50 (2) ◽  
pp. 151-162 ◽  
Author(s):  
C. A. CAMPBELL ◽  
W. S. FERGUSON ◽  
F. G. WARDER

Cylinders of a loam soil were placed in the field in late fall and sampled in midwinter and early spring. In soil wetted to near field capacity, nitrate and moisture moved upwards in winter and downwards again in early spring. The amount of movement was negligible in a soil wet to near the wilting percentage. To inhibit nitrification, N-serve was applied in 10 cm of water to field plots (120 × 120 cm) in late fall. Other plots received water but no N-serve. Regular monitoring of soil nitrate, exchangeable ammonium, and soil moisture and temperature in the top 90 cm of these plots showed evidence of upward moisture and nitrate movement as the soil froze. Large and sudden unexplainable decreases in exchangeable ammonium occurred following steady fall build-up.


2017 ◽  
Vol 8 (2) ◽  
pp. 293-298 ◽  
Author(s):  
J. H. Grove ◽  
E. M. Pena-Yewtukhiw

There is evidence that well managed winter cereal cover crops can scavenge a goodly amount of post summer cereal harvest residual nitrogen (N), reducing nitrate-N losses to leaching or runoff. The objective of this study was to compare nitrate-N phytoremediation areas derived from five sources of information: site specific, non-site specific, or a combination. The non-site specific source was a single “composite” soil nitrate sample. The site specific sources were: a) a dense soil nitrate-N grid sampling; and b) a N removal map calculated from yield and grain N concentration, both determined at the same grid density as soil nitrate-N. The source combinations were: a) a yield map and a single grain N concentration value taken from published information; and b) a yield map and a single field “composite” grain N concentration value. The results indicated that the published grain N value was inferior to measured grain N values, and that the maize (Zea mays L.) yield map best serves as a stratification tool, delineating similar crop performance areas. Random soil sampling within those areas further optimizes residual nitrate-N recovery management. Site specific technologies can guide establishment of N scavenging cover crops to simultaneously improve resource use efficiency and water quality.


1969 ◽  
Vol 20 ◽  
pp. 27-30 ◽  
Author(s):  
Flemming Jørgensen ◽  
Rasmus Rønde Møller ◽  
Peter B.E. Sandersen ◽  
Lars Nebel

Contamination of groundwater with pesticides and nitrate has compelled the Danish Government to launch a major hydrogeological mapping programme covering about 40% of the land area of Denmark. Numerous geophysical surveys are currently being carried out in order to acquire the necessary data. These new data are crucial for the 3-D geological models that are used in the planning of future water supply and landuse. Normally, site-specific groundwater protection zones (Thomsen et al. 2004) are based on groundwatermodelled catchment areas for each well, but proper 3-D geological models are needed in order to create a valid basis for the groundwater models. Since most of the Danish nearsurface geology is complex, a full geological understanding is required combined with in-depth interpretation of geological and geophysical data.


1999 ◽  
Vol 79 (1) ◽  
pp. 65-72 ◽  
Author(s):  
J. B. Sanderson ◽  
J. A. MacLeod ◽  
J. Kimpinski

The effects of glyphosate application and time of tillage of red clover on soil NO3-N profiles, the response of the subsequent potato crop to varying rates of N fertilizer application, and root and soil nematodes, were evaluated. The study was conducted over three cropping seasons on a Charlottetown fine sandy loam on Prince Edward Island. Red clover was moldboard ploughed in mid-September (early fall), or moldboard or chisel ploughed in mid-October (late fall), or moldboard ploughed in the spring. Glyphosate was applied in early fall and the dead clover was moldboard or chisel ploughed in mid-October or left undisturbed until spring. A barley control, where barley was harvested and straw incorporated with a tandem disc harrow in early September, was included. Potato were planted with 6 rates of N (0 to 250 kg ha–1 in 50-kg increments) band applied at planting. The barley control treatment produced lower potato yield without fertilizer N in all 3 yr of the study compared to red clover treatments, but potato yields were similar at high rates of applied N. Potato tuber yields after spring moldboard ploughing of red clover were higher at low N rates and lower at high N rates than yields after late fall treatment in one year of the study. Concentrations of nitrate-N in the soil in mid-November were highest following the early fall moldboard ploughing and lowest in the undisturbed clover plots. Fall soil nitrate-N levels were intermediate following the glyphosate application and late fall tillages. Early spring soil nitrate-N levels in the surface 30 cm were generally highest with the spring and late fall tillage and lowest with the early fall tillage. Levels of nitrate-N in potato petioles increased with increased rates of N application and generally increased as tillage of the red clover was delayed from early fall to late fall to spring. In general, cultivation and the application of glyphosate did not affect soil and root nematode populations. In two instances, the moldboard plough tillage treatments were associated with higher levels of the clover-cyst nematode, Heterodera trifolii. One of the tillage treatments was combined with glyphosate, but this was the only case where the herbicide had an impact on nematodes. To maximize the benefits to the subsequent potato crop and to minimize leaching of nitrate, incorporation of legume residue should be delayed until the rate of mineralization and nitrification of the legume N is minimized. It is suggested that incorporation of red clover be delayed until after mid-October for clover–potato systems on PEI. Key words: Glyphosate, nematodes, nitrate leaching, petiole nitrate-N, potato, soil nitrate-N, tillage time


Author(s):  
Richard D. Powell ◽  
James F. Hainfeld ◽  
Carol M. R. Halsey ◽  
David L. Spector ◽  
Shelley Kaurin ◽  
...  

Two new types of covalently linked, site-specific immunoprobes have been prepared using metal cluster labels, and used to stain components of cells. Combined fluorescein and 1.4 nm “Nanogold” labels were prepared by using the fluorescein-conjugated tris (aryl) phosphine ligand and the amino-substituted ligand in the synthesis of the Nanogold cluster. This cluster label was activated by reaction with a 60-fold excess of (sulfo-Succinimidyl-4-N-maleiniido-cyclohexane-l-carboxylate (sulfo-SMCC) at pH 7.5, separated from excess cross-linking reagent by gel filtration, and mixed in ten-fold excess with Goat Fab’ fragments against mouse IgG (obtained by reduction of F(ab’)2 fragments with 50 mM mercaptoethylamine hydrochloride). Labeled Fab’ fragments were isolated by gel filtration HPLC (Superose-12, Pharmacia). A combined Nanogold and Texas Red label was also prepared, using a Nanogold cluster derivatized with both and its protected analog: the cluster was reacted with an eight-fold excess of Texas Red sulfonyl chloride at pH 9.0, separated from excess Texas Red by gel filtration, then deprotected with HC1 in methanol to yield the amino-substituted label.


2020 ◽  
Vol 64 (1) ◽  
pp. 135-153 ◽  
Author(s):  
Lauren Elizabeth Smith ◽  
Adelina Rogowska-Wrzesinska

Abstract Post-translational modifications (PTMs) are integral to the regulation of protein function, characterising their role in this process is vital to understanding how cells work in both healthy and diseased states. Mass spectrometry (MS) facilitates the mass determination and sequencing of peptides, and thereby also the detection of site-specific PTMs. However, numerous challenges in this field continue to persist. The diverse chemical properties, low abundance, labile nature and instability of many PTMs, in combination with the more practical issues of compatibility with MS and bioinformatics challenges, contribute to the arduous nature of their analysis. In this review, we present an overview of the established MS-based approaches for analysing PTMs and the common complications associated with their investigation, including examples of specific challenges focusing on phosphorylation, lysine acetylation and redox modifications.


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