Registration of High Fiber Strength Cotton Germplasm Line NM970513

Cotton (Gossypium L.) is the most important fiber crop worldwide. Here, transcriptome analysis was conducted on developing fibers of a G. mustelinum introgression line, IL9, and its recurrent parent, PD94042, at 17 and 21 days post-anthesis (dpa). Differentially expressed genes (DEGs) of PD94042 and IL9 were identified. Gene Ontology (GO) enrichment analysis showed that the annotated DEGs were rich in two main biological processes and two main molecular functions. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis likewise showed that the annotated DEGs were mainly enriched in metabolic pathways and biosynthesis of secondary metabolites. In total, 52 DEGs were selected as candidate genes based on comparison of the DEGs and GO function annotation information. Quantitative real-time PCR (RT-qPCR) analysis results for 12 randomly selected DEGs were consistent with transcriptome analysis. SNP identification based on G. mustelinum chromatin segment introgression showed that 394 SNPs were identified in 268 DEGs, and two genes with known functions were identified within fiber strength quantitative trait loci (QTL) regions or near the confidence intervals. We identified 52 key genes potentially related to high fiber strength in a G. mustelinum introgression line and provided significant insights into the study of cotton fiber quality improvement.

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Tracking tendon fibers to their insertion – a 3D analysis of the Achilles tendon enthesis in mice

10.1101/2020.02.25.964759 ◽

2020 ◽

Author(s):

Julian Sartori ◽

Heiko Stark

Keyword(s):

Achilles Tendon ◽

Fiber Strength ◽

Homogeneous Distribution ◽

3D Analysis ◽

Cross Sectional ◽

High Fiber ◽

Distinct Portion ◽

Fiber Number ◽

Low Levels ◽

Distribution Of Stress

AbstractTendon insertions to bone are heavily loaded transitions between soft and hard tissues. The fiber courses in the tendon have profound effects on the distribution of stress along and across the insertion. We tracked fibers of the Achilles tendon in mice in micro-computed tomographies and extracted virtual transversal sections. The fiber tracks and shapes were analyzed from a position in the free tendon to the insertion with regard to their mechanical consequences. The fiber number was found to stay about constant along the tendon. But the fiber cross-sectional areas decrease towards the insertion. The fibers mainly interact due to tendon twist, while branching only creates small branching clusters with low levels of divergence along the tendon. The highest fiber curvatures were found within the unmineralized entheseal fibrocartilage. The fibers inserting at a protrusion of the insertion area form a distinct portion within the tendon. Tendon twist is expected to contribute to a homogeneous distribution of stress among the fibers. According to the low cross-sectional areas and the high fiber curvatures, tensile and compressive stress are expected to peak at the insertion. These findings raise the question whether the insertion is reinforced in terms of fiber strength or by other load-bearing components besides the fibers.

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Microanalyses Of Structural Defects In Polymer-Derived Ceramic Fibers

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100134910 ◽

1990 ◽

Vol 48 (2) ◽

pp. 262-263

Author(s):

H. A. Freeman ◽

J. A. Rabe

Keyword(s):

Surface Defects ◽

Fiber Strength ◽

Thermal Exposure ◽

Ceramic Composites ◽

Silicon Oxynitride ◽

Structural Defects ◽

Ceramic Fibers ◽

High Fiber ◽

Polymer Derived Ceramic ◽

Primary Fracture

Microanalyses were performed with a Cameca MBX scanning electron microprobe and a JEM 2000FX analytical electron microscope to determine the composition of foreign defect structures found on primary fracture surfaces of ceramic fibers derived from melt-spun Si-N-C-O polymeric precursors. The defects were the critical flaws responsible for deterioration of tensile strength in these fibers after thermal exposure in the range of 1400°C and above. High fiber strength is necessary to provide reinforcement and toughening in structural ceramic composites during high temperature use.Among the defects found were roughly spherical internal nodular flaws which contained Pb (Fig. 1) as well as others (Fig. 2) containing both Pb and Sn. Granular aggregates were also seen in which Fe and Cr were detected. Other nodular surface defects contained Ca, minor Al, and levels of 0 higher than that in the surrounding fiber (Fig. 3) . Extremely fine whisker growths at discrete sites along the length of some fibers were identified as silicon oxynitride by electron diffraction.

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