SNP‐Based Improvement of a Microsatellite Marker Associated with Karnal Bunt Resistance in Wheat

Abstract Utilization of modern breeding techniques for developing high yielding and uniform plant types ultimately narrowing the genetic makeup of most crops. Narrowed genetic makeup of these crops has made them vulnerable towards disease and insect epidemics. For sustainable crop production, genetic variability of these crops must be broadened against various biotic and abiotic stresses. One of the ways to widen genetic configuration of these crops is to identify novel additional sources of durable resistance. In this regard crops wild relatives are providing valuable sources of allelic diversity towards various biotic, abiotic stress tolerance and quality components. For incorporating novel variability from wild relative’s wide hybridization technique has become a promising breeding method. For this purpose, wheat-Th. bessarabicum amphiploid, addition and translocation lines have been screened in field and screen house conditions to get novel sources of yellow rust and Karnal bunt resistant. Stripe rust screening under field conditions has revealed addition lines 4JJ and 6JJ as resistant to moderately resistant while addition lines 3JJ, 5JJ, 7JJ and translocation lines Tr-3, Tr-6 as moderately resistant wheat-Thinopyrum-bessarabicum genetic stock. Karnal bunt screening depicted addition lines 5JJ and 4JJ as highly resistant genetic stock. These genetic stocks may be used to introgression novel stripe rust and Karnal bunt resistance from the tertiary gene pool into susceptible wheat backgrounds.

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Immunological characterization of Karnal bunt resistance by differential response of pathogen restriction in a dual culture system

Food and Agricultural Immunology ◽

10.1080/09540100400016024 ◽

2005 ◽

Vol 16 (1) ◽

pp. 59-71

Author(s):

N. Sethi ◽

G. K. Garg ◽

U. S. Singh ◽

J. P. Jaiswal ◽

A. Kumar

Keyword(s):

Culture System ◽

Differential Response ◽

Karnal Bunt ◽

Dual Culture ◽

Immunological Characterization ◽

Bunt Resistance

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Molecular mapping of QTLs for Karnal bunt resistance in two recombinant inbred populations of bread wheat

Theoretical and Applied Genetics ◽

10.1007/s00122-007-0654-6 ◽

2007 ◽

Vol 116 (1) ◽

pp. 147-154 ◽

Cited By ~ 12

Author(s):

Sukhwinder Singh ◽

Indu Sharma ◽

Sunish K. Sehgal ◽

Navtej S. Bains ◽

Zhigang Guo ◽

...

Keyword(s):

Bread Wheat ◽

Recombinant Inbred ◽

Molecular Mapping ◽

Karnal Bunt ◽

Inbred Populations ◽

Bunt Resistance

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Pre-emptive Breeding Against Karnal Bunt Infection in Common Wheat: Combining Genomic and Agronomic Information to Identify Suitable Parents

Frontiers in Plant Science ◽

10.3389/fpls.2021.675859 ◽

2021 ◽

Vol 12 ◽

Author(s):

Livinus Emebiri ◽

Shane Hildebrand ◽

Mui-Keng Tan ◽

Philomin Juliana ◽

Pawan K. Singh ◽

...

Keyword(s):

Agronomic Traits ◽

Wheat Variety ◽

Cost Effective ◽

Karnal Bunt ◽

World Population ◽

Gwas Study ◽

Genome Wide ◽

A Genome ◽

Ideal Genotype ◽

Bunt Resistance

Wheat (Triticum aestivum L.) is the most widely grown cereal crop in the world and is staple food to half the world’s population. The current world population is expected to reach 9.8 billion people by 2050, but food production is not expected to keep pace with demand in developing countries. Significant opportunities exist for traditional grain exporters to produce and export greater amounts of wheat to fill the gap. Karnal bunt, however, is a major threat, due to its use as a non-tariff trade barrier by several wheat-importing countries. The cultivation of resistant varieties remains the most cost-effective approach to manage the disease, but in countries that are free of the disease, genetic improvement is difficult due to quarantine restrictions. Here we report a study on pre-emptive breeding designed to identify linked molecular markers, evaluate the prospects of genomic selection as a tool, and prioritise wheat genotypes suitable for use as parents. In a genome-wide association (GWAS) study, we identified six DArTseq markers significantly linked to Karnal bunt resistance, which explained between 7.6 and 29.5% of the observed phenotypic variation. The accuracy of genomic prediction was estimated to vary between 0.53 and 0.56, depending on whether it is based solely on the identified Quantitative trait loci (QTL) markers or the use of genome-wide markers. As genotypes used as parents would be required to possess good yield and phenology, further research was conducted to assess the agronomic value of Karnal bunt resistant germplasm from the International Maize and Wheat Improvement Center (CIMMYT). We identified an ideal genotype, ZVS13_385, which possessed similar agronomic attributes to the highly successful Australian wheat variety, Mace. It is phenotypically resistant to Karnal bunt infection (<1% infection) and carried all the favourable alleles detected for resistance in this study. The identification of a genotype combining Karnal bunt resistance with adaptive agronomic traits overcomes the concerns of breeders regarding yield penalty in the absence of the disease.

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Additive genes at nine loci govern Karnal bunt resistance in a set of common wheat cultivars

Euphytica ◽

10.1007/s10681-005-2436-z ◽

2005 ◽

Vol 142 (3) ◽

pp. 301-307 ◽

Cited By ~ 8

Author(s):

Indu Sharma ◽

Navtej Singh Bains ◽

Kuldeep Singh ◽

Govinder Singh Nanda

Keyword(s):

Common Wheat ◽

Karnal Bunt ◽

Wheat Cultivars ◽

Additive Genes ◽

Bunt Resistance

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Genome Wide Association Study of Karnal Bunt Resistance in a Wheat Germplasm Collection from Afghanistan

International Journal of Molecular Sciences ◽

10.3390/ijms20133124 ◽

2019 ◽

Vol 20 (13) ◽

pp. 3124 ◽

Cited By ~ 5

Author(s):

Vikas Gupta ◽

Xinyao He ◽

Naresh Kumar ◽

Guillermo Fuentes-Davila ◽

Rajiv K. Sharma ◽

...

Keyword(s):

Association Study ◽

Genome Wide Association Study ◽

Germplasm Collection ◽

Resistance Breeding ◽

Snp Markers ◽

Genome Wide Association ◽

Karnal Bunt ◽

Genome Wide ◽

Genomic Regions ◽

Bunt Resistance

Karnal bunt disease of wheat, caused by the fungus Neovossia indica, is one of the most important challenges to the grain industry as it affects the grain quality and also restricts the international movement of infected grain. It is a seed-, soil- and airborne disease with limited effect of chemical control. Currently, this disease is contained through the deployment of host resistance but further improvement is limited as only a few genotypes have been found to carry partial resistance. To identify genomic regions responsible for resistance in a set of 339 wheat accessions, genome-wide association study (GWAS) was undertaken using the DArTSeq® technology, in which 18 genomic regions for Karnal bunt resistance were identified, explaining 5–20% of the phenotypic variation. The identified quantitative trait loci (QTL) on chromosome 2BL showed consistently significant effects across all four experiments, whereas another QTL on 5BL was significant in three experiments. Additional QTLs were mapped on chromosomes 1DL, 2DL, 4AL, 5AS, 6BL, 6BS, 7BS and 7DL that have not been mapped previously, and on chromosomes 4B, 5AL, 5BL and 6BS, which have been reported in previous studies. Germplasm with less than 1% Karnal bunt infection have been identified and can be used for resistance breeding. The SNP markers linked to the genomic regions conferring resistance to Karnal bunt could be used to improve Karnal bunt resistance through marker-assisted selection.

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