The Calcium Binding Protein S100β Marks Hedgehog-Responsive Perivascular Stem Cells That Contribute to Intimal Thickening Following Iatrogenic Flow Restriction

2020 ◽  
Author(s):  
Mariana Di Luca ◽  
Emma Fitzpatrick ◽  
Denise Burtenshaw ◽  
Weimin Liu ◽  
Jay-Christian Helt ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Intimal Thickening ◽  
Flow Restriction ◽  
Perivascular Stem Cells

scholarly journals The calcium binding protein S100β marks Hedgehog-responsive perivascular stem cells that contribute to intimal thickening following iatrogenic flow restriction

2020 ◽  
Author(s):  
Mariana Di Luca ◽  
Emma Fitzpatrick ◽  
Denise Burtenshaw ◽  
Weimin Liu ◽  
Jay-Christian Helt ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Binding ◽  
Myogenic Differentiation ◽  
Subclinical Atherosclerosis ◽  
Lineage Tracing ◽  
Genetic Lineage ◽  
Intimal Thickening ◽  
Lesion Formation ◽  
Flow Restriction

SummaryA hallmark of subclinical atherosclerosis is the accumulation of vascular smooth muscle cell (SMC)-like cells leading to intimal thickening. While medial SMCs contribute, the participation of hedgehog responsive resident vascular stem cells (vSCs) to lesion formation remains unclear. Using transgenic eGFP mice and genetic lineage tracing of S100β vSCs in vivo, we identified S100β/Sca1 cells derived from a perivascular S100β non-SMC parent population within lesions following iatrogenic flow restriction, an effect attenuated following hedgehog inhibition with cyclopamine. Multipotent S100β/Sca1 vSCs acquired the stable SMC epigenetic H3K4me2 histone mark at the Myh11 locus and underwent myogenic differentiation in response to sonic hedgehog (SHh) in vitro. S100β cells were enriched in human arteriosclerotic lesions while SHh promoted myogenic differentiation of human induced pluripotent stem cell derived S100β neuroectoderm progenitors in vitro. We conclude that hedgehog responsive S100β vSCs contribute to lesion formation and support targeting hedgehog signalling to treat subclinical arteriosclerosis.

Calcium-binding protein 1 of Entamoeba histolytica transiently associates with phagocytic cups in a calcium-independent manner

2008 ◽  
Vol 10 (6) ◽  
pp. 1373-1389 ◽  
Author(s):  
Ruchi Jain ◽  
Julien Santi-Rocca ◽  
Narendra Padhan ◽  
Sudha Bhattacharya ◽  
Nancy Guillen ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Independent Manner ◽  
Phagocytic Cups

scholarly journals Regulation of adenylate cyclase from glial tumor cells by calcium and a calcium-binding protein.

1976 ◽  
Vol 251 (15) ◽  
pp. 4744-4750 ◽  
Author(s):  
M A Brostrom ◽  
C O Brostrom ◽  
B M Breckenridge ◽  
D J Wolff
Keyword(s):  
Adenylate Cyclase ◽  
Tumor Cells ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Glial Tumor
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