Superiority of Leukemic Stem Cell-Based Minimal Residual Disease Assay to Traditional Multiparameter Flow Cytometry-Based Method for Relapse Prediction in AML Patients: A Prospective Study with Randomized Training and Validation Sets

2021 ◽  
Author(s):  
Si-Qi Li ◽  
Lan-Ping Xu ◽  
Yu Wang ◽  
Xiao-Hui Zhang ◽  
Huan Chen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Stem Cell ◽  
Prospective Study ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Multiparameter Flow Cytometry ◽  
Leukemic Stem Cell ◽  
Relapse Prediction ◽  
A Prospective Study ◽  
Minimal Residual

scholarly journals Multiparameter flow cytometry for assessment of minimal residual disease in patients with myelodysplastic syndromes treated with allogeneic stem cell transplantation

2020 ◽  
Vol 51 (2) ◽  
pp. 88-94
Author(s):  
Katarzyna Wiśniewska-Piąty ◽  
Joanna Dziaczkowska-Suszek ◽  
Agata Wieczorkiewicz-Kabut ◽  
Karolina Chromik ◽  
Anna Koclęga ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Minimal Residual Disease ◽  
Cell Transplantation ◽  
Allogeneic Stem Cell Transplantation ◽  
Myelodysplastic Syndromes ◽  
Residual Disease ◽  
Multiparameter Flow Cytometry ◽  
Minimal Residual

AbstractBackgroundMyelodysplastic syndromes (MDSs) are a heterogeneous group of clonal myeloid neoplasms. Allogeneic stem cell transplantation (allo-SCT) remains the curative method for MDS treatment. Little is known about the monitoring of minimal residual disease (MRD) in patients with MDS after allo-SCT.AimWe aimed to evaluate the significance of leukemia-associated immunophenotypes (LAIPs) identified in acute myeloid leukemia (AML) for MRD monitoring in patients with MDS after allo-SCT.Material and methodsSeven males and 4 females with a median age of 55 years were included. The diagnosis of MDS was established according to 2016 World Health Organization (WHO) criteria. The significance of eight LAIPs in bone marrow samples using multiparameter flow cytometry (MFC) was evaluated for MRD.ResultsEight patients were positive for several LAIPs before allo-SCT. The identified LAIPs included the presence of aberrant lymphoid antigens on myeloblasts and lack of CD33 expression on myeloblasts. All studied MDS patients were negative for LAIPs at Day +30 after the procedure. This was followed by full-donor chimerism in all cases. The Ogata score after allo-SCT decreased in all patients in whom it was indicative for MDS before allo-SCT.ConclusionsMFC could be useful in monitoring MRD in MDS patients after allo-SCT. Further studies in this field are needed.

scholarly journals High-risk cytogenetics and persistent minimal residual disease by multiparameter flow cytometry predict unsustained complete response after autologous stem cell transplantation in multiple myeloma

Blood ◽  
2012 ◽  
Vol 119 (3) ◽  
pp. 687-691 ◽  
Author(s):  
Bruno Paiva ◽  
Norma C. Gutiérrez ◽  
Laura Rosiñol ◽  
María-Belén Vídriales ◽  
María-Ángeles Montalbán ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Flow Cytometry ◽  
Stem Cell ◽  
High Risk ◽  
Stem Cell Transplantation ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Complete Response ◽  
Multiparameter Flow Cytometry ◽  
Minimal Residual

Abstract The achievement of complete response (CR) after high-dose therapy/autologous stem cell transplantation (HDT/ASCT) is a surrogate for prolonged survival in multiple myeloma; however, patients who lose their CR status within 1 year of HDT/ASCT (unsustained CR) have poor prognosis. Thus, the identification of these patients is highly relevant. Here, we investigate which prognostic markers can predict unsustained CR in a series of 241 patients in CR at day +100 after HDT/ASCT who were enrolled in the Spanish GEM2000 (n = 140) and GEM2005 < 65y (n = 101) trials. Twenty-nine (12%) of the 241 patients showed unsustained CR and a dismal outcome (median overall survival 39 months). The presence of baseline high-risk cytogenetics by FISH (hazard ratio 17.3; P = .002) and persistent minimal residual disease by multiparameter flow cytometry at day +100 after HDT/ASCT (hazard ratio 8.0; P = .005) were the only independent factors that predicted unsustained CR. Thus, these 2 parameters may help to identify patients in CR at risk of early progression after HDT/ASCT in whom novel treatments should be investigated.

scholarly journals Persistence of minimal residual disease assessed by multiparameter flow cytometry is highly prognostic in younger patients with acute myeloid leukemia

Cancer ◽  
2016 ◽  
Vol 123 (3) ◽  
pp. 426-435 ◽  
Author(s):  
Farhad Ravandi ◽  
Jeffrey Jorgensen ◽  
Gautam Borthakur ◽  
Elias Jabbour ◽  
Tapan Kadia ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Acute Myeloid Leukemia ◽  
Minimal Residual Disease ◽  
Myeloid Leukemia ◽  
Residual Disease ◽  
Multiparameter Flow Cytometry ◽  
Younger Patients ◽  
Minimal Residual ◽  
Acute Myeloid

scholarly journals A probabilistic approach for the evaluation of minimal residual disease by multiparameter flow cytometry in leukemic B-cell chronic lymphoproliferative disorders

2008 ◽  
Vol 73A (12) ◽  
pp. 1141-1150 ◽  
Author(s):  
C.E. Pedreira ◽  
E.S. Costa ◽  
J. Almeida ◽  
C. Fernandez ◽  
S. Quijano ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
B Cell ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Probabilistic Approach ◽  
Lymphoproliferative Disorders ◽  
Multiparameter Flow Cytometry ◽  
Minimal Residual

A prospective study of minimal residual disease in patients with diffuse large B-cell lymphoma using an Ig-NGS assay

2020 ◽  
pp. 1-4
Author(s):  
Matthew L. Chase ◽  
Reid Merryman ◽  
David C. Fisher ◽  
Eric Jacobsen ◽  
Ann LaCasce ◽  
...  
Keyword(s):  
Prospective Study ◽  
B Cell ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Large B Cell Lymphoma ◽  
A Prospective Study ◽  
Minimal Residual ◽  
Large B Cell
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