Metagenomic Analysis of Microbial Community Structure and Distribution of Resistance Gene in the Daihai Lake, China

2021 ◽  
Author(s):  
Cai-li Du ◽  
Fang Yang ◽  
Xiao-guang Li ◽  
Haiqing Liao ◽  
Zhong-hong Li ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Resistance Gene ◽  
Microbial Community Structure ◽  
Metagenomic Analysis ◽  
Daihai Lake

scholarly journals Comparative Metagenomic Analysis of Chicken Gut Microbial Community, Function, and Resistome to Evaluate Noninvasive and Cecal Sampling Resources

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1718
Author(s):  
Kelang Kang ◽  
Yan Hu ◽  
Shu Wu ◽  
Shourong Shi
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Fecal Microbiota ◽  
Metagenomic Analysis ◽  
Fecal Samples ◽  
Microbial Community Function ◽  
Community Function ◽  
Rectal Swabs ◽  
Gut Microbial Community

When conducting metagenomic analysis on gut microbiomes, there is no general consensus concerning the mode of sampling: non-contact (feces), noninvasive (rectal swabs), or cecal. This study aimed to determine the feasibility and comparative merits and disadvantages of using fecal samples or rectal swabs as a proxy for the cecal microbiome. Using broiler as a model, gut microbiomes were obtained from cecal, cloacal, and fecal samples and were characterized according to an analysis of the microbial community, function, and resistome. Cecal samples had higher microbial diversity than feces, while the cecum and cloaca exhibited higher levels of microbial community structure similarity compared with fecal samples. Cecal microbiota possessed higher levels of DNA replicative viability than feces, while fecal microbiota were correlated with increased metabolic activity. When feces were excreted, the abundance of antibiotic resistance genes like tet and ErmG decreased, but some antibiotic genes became more prevalent, such as fexA, tetL, and vatE. Interestingly, Lactobacillus was a dominant bacterial genus in feces that led to differences in microbial community structure, metabolism, and resistome. In conclusion, fecal microbiota have limited potential as a proxy in chicken gut microbial community studies. Thus, feces should be used with caution for characterizing gut microbiomes by metagenomic analysis.

scholarly journals Metagenomic analysis of the microbial community structure in protected wetlands in the Maritza River Basin

2019 ◽  
Vol 33 (1) ◽  
pp. 1721-1732 ◽  
Author(s):  
Ivan Iliev ◽  
Mariana Marhova ◽  
Sonya Kostadinova ◽  
Velizar Gochev ◽  
Marinela Tsankova ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
River Basin ◽  
Microbial Community Structure ◽  
Metagenomic Analysis

Microbial community and antibiotic resistance profiles of biomass and effluent are distinctly affected by antibiotic addition to an anaerobic membrane bioreactor

2020 ◽  
Vol 6 (3) ◽  
pp. 724-736 ◽  
Author(s):  
Ali Zarei-Baygi ◽  
Moustapha Harb ◽  
Phillip Wang ◽  
Lauren B. Stadler ◽  
Adam L. Smith
Keyword(s):  
Antibiotic Resistance ◽  
Community Structure ◽  
Microbial Community ◽  
Resistance Gene ◽  
Microbial Community Structure ◽  
Membrane Bioreactor ◽  
Antibiotic Resistance Gene ◽  
Domestic Wastewater ◽  
Bench Scale ◽  
Anaerobic Membrane Bioreactor

A bench-scale AnMBR was operated for the treatment of domestic wastewater containing antibiotics and evaluated microbial community structure and antibiotic resistance gene dynamics in both the biomass and effluent.

scholarly journals Metagenomic Analysis of Microbial Community Structure And Function In A Improved Biofilter With Odorous Gases

2021 ◽  
Author(s):  
Jianguo Ni ◽  
Huayun Yang ◽  
Liqing Chen ◽  
Jiadong Xu ◽  
Liangwei Zheng ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Metabolic Pathway ◽  
Structure And Function ◽  
Metagenomic Analysis ◽  
Industrial Emissions ◽  
Industrial Enterprises ◽  
Before And After ◽  
And Function

Abstract Biofilters have been broadly applied to degrade the odorous gases from industrial emissions. A industrial scale biofilter was set up to treat the odorous gases. To explore biofilter potentials, the microbial community structure and function must be well defined. Using of improved biofilter, the differences in microbial community structures and functions in biofilters before and after treatment were investigated by metagenomic analysis. Odorous gases have the potential to alter the microbial community structure in the sludge of biofilter. A total of 90,016 genes assigned into various functional metabolic pathways were identified. In the improved biofilter, the dominant phyla were Proteobacteria, Planctomycetes, and Chloroflexi, and the dominant genera were Thioalkalivibrio, Thauera, and Pseudomonas. Several xenobiotic biodegradation-related pathways showed significant changes during the treatment process. Compared with the original biofilter, Thermotogae and Crenarchaeota phyla were significantly enriched in the improved biofilter, suggesting their important role in nitrogen-fixing. Furthermore, several nitrogen metabolic pathway-related genes, such as nirA and nifA, and sulfur metabolic pathway-related genes, such as fccB and phsA, were considered to be efficient genes that were involved in removing odorous gases. Our findings can be used for improving the efficiency of biofilter and helping the industrial enterprises to reduce the emission of waste gases.

Marine microbial community structure assessed from combined metagenomic analysis and ribosomal amplicon deep-sequencing

2015 ◽  
Vol 12 (1) ◽  
pp. 30-42 ◽  
Author(s):  
Savvas Genitsaris ◽  
Sebastien Monchy ◽  
Jeremie Denonfoux ◽  
Stephanie Ferreira ◽  
Konstantinos Ar. Kormas ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Deep Sequencing ◽  
Microbial Community Structure ◽  
Metagenomic Analysis

Study on the differences in sludge toxicity and microbial community structure caused by catechol, resorcinol and hydroquinone with metagenomic analysis

2022 ◽  
Vol 302 ◽  
pp. 114027
Author(s):  
Xiurong Chen ◽  
Xueyang Hu ◽  
Quanling Lu ◽  
Yingying Yang ◽  
Shanshan Linghu ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Metagenomic Analysis

scholarly journals Metagenomic analysis of microbial community structure and function in a improved biofilter with odorous gases

2021 ◽  
Author(s):  
Jianguo Ni ◽  
Liqing Chen ◽  
Jiadong Xu ◽  
Liangwei Zheng ◽  
Guojian Xie ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Metabolic Pathway ◽  
Structure And Function ◽  
Metagenomic Analysis ◽  
Industrial Emissions ◽  
Waste Gas ◽  
Industrial Enterprises ◽  
And Function

Abstract BackgroundBiofilters have been broadly applied to degrade the odorous gases from industrial emissions. To explore biofilter potentials, the microbial community structure and function must be well defined. Using of improved biofilter, changes in microbial community structures and functions were investigated by metagenomic analysis. ResultsOdorous gases have the potential to alter the microbial community structure in the sludge of biofilter. A total of 90,016 unigenes assigned into various functional metabolic pathways were identified. In the improved biofilter, the dominant phyla were Proteobacteria, Planctomycetes, and Chloroflexi, and the dominant were Thioalkalivibrio, Thauera, and Pseudomonas. Several xenobiotic biodegradation-related pathways showed significant changes during the treatment process. Compared with the original biofilter, Thermotogae and Crenarchaeota phyla were significantly up-regulated in the improved biofilter, suggesting its important role in nitrogen-fixing. Furthermore, nitrogen metabolic pathway-related genes, such as nirA and nifA, and sulfur metabolic pathway-related genes, such as fccB and phsA, were considered to be efficient genes involved in removing odorous gases. ConclusionsOur findings can be used for improving the efficiency of biofilter and helping the industrial enterprises to reduce the emission of waste gas.

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