1G1536 Atmospheric scanning electron microscopy (ASEM) directly observes protein microcrystals in liquid(Protein: Structure 1,The 49th Annual Meeting of the Biophysical Society of Japan)

Mutations in the human amelogenin gene (AMELX, Xp22.3) cause a phenotypically diverse set of inherited enamel malformations. We hypothesize that the effects of specific mutations on amelogenin protein structure and expression will correlate with the enamel phenotype, clarify amelogenin structure/function relationships, and improve the clinical diagnosis of X-linked amelogenesis imperfecta (AI). We have identified two kindreds with X-linked AI and characterized the AMELX mutations underlying their AI phenotypes. The two missense mutations are both in exon 2 and affect the translation initiation codon and/or the secretion of amelogenin (p.M1T and p.W4S), resulting in hypoplastic enamel. Primary anterior teeth from affected females with the p.M1T mutation were characterized by light and scanning electron microscopy. The thin enamel had defective prism organization, and the surface was rough and pitted. Dentin was normal. The severity of the enamel phenotype correlated with the predicted effects of the mutations on amelogenin expression and secretion.

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2P019 Structural analysis of needle complex from shigella flexneri by cryo electron microscopy(01A. Protein: Structure,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Seibutsu Butsuri ◽

10.2142/biophys.54.s198_1 ◽

2014 ◽

Vol 54 (supplement1-2) ◽

pp. S198

Author(s):

Naoko Kajimura ◽

Fumiaki Makino ◽

Martin P Cheung ◽

Takayuki Kato ◽

Ariel J Blocker ◽

...

Keyword(s):

Electron Microscopy ◽

Protein Structure ◽

Structural Analysis ◽

Annual Meeting ◽

Shigella Flexneri ◽

Cryo Electron Microscopy ◽

Biophysical Society

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Non-Thrombogenic Characteristics of Hybrid Vascular Prostheses

10.1055/s-0039-1684580 ◽

1979 ◽

Author(s):

P.N. Sawyer ◽

B. Stanczewski

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Protein Structure ◽

Thrombus Formation ◽

Vascular Prosthesis ◽

Short Term ◽

Vascular Prostheses ◽

Complete Occlusion ◽

Dacron Prostheses ◽

Scanning Electron

In our continuing efforts to develop a compound, coated vascular prosthesis, combining the best characteristics of biologic and nonbiologic materials, we have produced a hybri graft with pseudo biological coating. Five types of velour and knit dacron prostheses, have been cross linked subsequently with negatively charged proteins. Albumin and gelatin were used to coat the prosthesis or the combination of both. Following electrochemical characterization coated prostheses were implanted into the carotid and femoral arteries of dogs for 1 sec. 2 hr. and 1 mo. Preparations that showed promising short term results were implanted in the abdominal aorta of dogs for 18 months. Controls included uncoated prostheses and prostheses that had been coated but not negatively charged. Upon removal, the grafts were examined for thrombi and other luminal narrowings (photography, light and scanning electron microscopy). Fifty two uncoated and coated grafts have been examined. These exhibit a range of thrombus formation, from none to complete occlusion, in the various knit/coating combinations. We believe these hybrid prostheses will prove to be very useful in vascular reconstructive surgery due to (i) the nonthrombogenic characteristics of the negatively charged protein structure, (ii) more uniform healing and (iii) ease of implantation since preclotting is not necessary.

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Pathogenesis of Human Hair Defects

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010005007x ◽

1974 ◽

Vol 32 ◽

pp. 12-13

Author(s):

P.S. Porter ◽

T. Aoyagi ◽

R. Matta

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Human Hair ◽

Standard Techniques ◽

Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

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Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100080614 ◽

1977 ◽

Vol 35 ◽

pp. 638-639

Author(s):

P.J. Dailey

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Salivary Glands ◽

Secretory Vesicles ◽

The Past ◽

Transmission Electron ◽

Means Of Transmission ◽

Gromphadorhina Portentosa ◽

Scanning Electron ◽

Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

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Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100081279 ◽

1978 ◽

Vol 36 (2) ◽

pp. 82-83 ◽

Cited By ~ 1

Author(s):

Nakazo Watari ◽

Yasuaki Hotta ◽

Yoshio Mabuchi

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Transmission Electron Microscopy ◽

Fine Structure ◽

Light Microscopy ◽

Thin Sections ◽

Transmission Electron ◽

Identical Cell ◽

Electron Microscopes ◽

Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

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