scholarly journals An efficient and novel technology for the extraction of parasite genomic DNA from whole blood or culture

BioTechniques ◽  
2020 ◽  
Vol 68 (2) ◽  
pp. 79-84 ◽  
Author(s):  
David J Clark ◽  
Catherine M Moore ◽  
Marc Flanagan ◽  
Katrien Van Bocxlaer ◽  
Evangelia-Theophano Piperaki ◽  
...  
Keyword(s):  
Whole Blood ◽  
Genomic Dna ◽  
Leishmania Donovani ◽  
Small Volume ◽  
Small Sample ◽  
Blood Samples ◽  
Novel Technology ◽  
Spin Column ◽  
Whole Blood Samples ◽  
Pathogen Dna

The aim of this study was to assess pathogen DNA extraction with a new spin column-based method (DNA-XT). DNA from either whole-blood samples spiked with Plasmodium falciparum or  Leishmania donovani amastigote culture was extracted with DNA-XT and compared with that produced by a commercial extraction kit (DNeasy®). Eluates from large and small sample volumes were assessed by PCR and spectroscopy. Using a small volume (5 μl) of blood, the DNA-XT and DNeasy methods produced eluates with similar DNA concentrations (0.63 vs 1.06 ng/μl, respectively). The DNA-XT method produced DNA with lower PCR inhibition than DNeasy. The new technique was also twice as fast and required fewer plastics and manipulations but had reduced total recovered DNA compared with DNeasy.

scholarly journals A protocol for extraction of total RNA from finger stick whole blood samples preserved with TempusTM solution

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1739 ◽  
Author(s):  
Basirudeen Syed Ahamed Kabeer ◽  
Sara Tomei ◽  
Valentina Mattei ◽  
Tobias Brummaier ◽  
Rose McGready ◽  
...  
Keyword(s):  
Gene Expression ◽  
Whole Blood ◽  
Small Volume ◽  
Rna Extraction ◽  
Cellular Mechanisms ◽  
Blood Samples ◽  
Total Rna ◽  
Low Volume ◽  
Whole Blood Samples ◽  
Finger Stick

Monitoring of blood transcriptional changes during disease or treatment could improve the understanding of cellular mechanisms associated with that particular condition. This can be achieved through serial sampling of small blood volumes. However, molecular analysis of gene expression from low volume samples remains a challenging task. To address this issue, we have developed a set of standard operating procedures (SOP), starting from collection of small volume blood to measurement of gene expression. Previously we published an SOP for the collection of a small volume of blood via finger stick and stabilization of RNA. The aim of this manuscript is to share a modified TempusTM solution based RNA extraction method, developed in our lab, for the extraction of total RNA from low volume whole blood samples collected via finger stick.

scholarly journals Hepatitis C Virus RNA Quantitation in Venous and Capillary Small-Volume Whole-Blood Samples

2009 ◽  
Vol 47 (10) ◽  
pp. 3231-3240 ◽  
Author(s):  
T. Bruns ◽  
K. Steinmetzer ◽  
E. Ermantraut ◽  
A. Stallmach
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Whole Blood ◽  
Small Volume ◽  
Hepatitis C Virus Rna ◽  
Blood Samples ◽  
Virus Rna ◽  
Whole Blood Samples

scholarly journals Comparison of commercial DNA extraction kits for the detection of bacterial genomic DNA from whole-blood samples using a broad-range PCR

Critical Care ◽  
2009 ◽  
Vol 13 (Suppl 4) ◽  
pp. P13
Author(s):  
B Krulova ◽  
E Nemcova ◽  
B Zaloudikova ◽  
P Nemec ◽  
T Freiberger
Keyword(s):  
Dna Extraction ◽  
Whole Blood ◽  
Genomic Dna ◽  
Blood Samples ◽  
Whole Blood Samples

Hepatitis C virus (HCV) RNA quantitation in small-volume whole blood samples

2009 ◽  
Vol 47 (01) ◽  
Author(s):  
T Bruns ◽  
K Steinmetzer ◽  
E Ermantraut ◽  
A Stallmach
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Whole Blood ◽  
Small Volume ◽  
Hcv Rna ◽  
Blood Samples ◽  
Whole Blood Samples
Sign in / Sign up

Export Citation Format

Share Document