scholarly journals High-purity DNA extraction from animal tissue using picking in the TRIzol-based method

BioTechniques ◽  
2020 ◽  
Author(s):  
Ye Yin Bo ◽  
Li Dong Liang ◽  
Yang Jiang Hua ◽  
Zhang Zhao ◽  
Mei Si Yao ◽  
...  
Keyword(s):  
Density Ratio ◽  
Spectrophotometric Analysis ◽  
Enzyme Digestion ◽  
Animal Tissue ◽  
Degraded Dna ◽  
Restriction Enzyme Digestion ◽  
Modified Method ◽  
Optical Density Ratio ◽  
Rna Contamination ◽  
Better Than

TRIzol is used for the extraction of RNA, DNA and proteins from tissues or cells. Here, we present a simple picking method to extract DNA from tissues using TRIzol. Spectrophotometric analysis showed that the 260/280 and 260/230 nm optical density ratio of the picking method's DNA is ideal and better than that obtained by the classic TRIzol method. Gel electrophoresis showed that there was no RNA contamination, and the DNA had not degraded. DNA extracted by the picking method had the same performance in restriction enzyme digestion and quantitative PCR as that obtained by the traditional method. Viral DNA in the infected tissue was also obtained. This modified method facilitates various molecular biology assays.

scholarly journals An In-Depth Assessment of the New BDS-3 B1C and B2a Signals

2021 ◽  
Vol 13 (4) ◽  
pp. 788
Author(s):  
Qinghua Zhang ◽  
Yongxing Zhu ◽  
Zhengsheng Chen
Keyword(s):  
Carrier Phase ◽  
Density Ratio ◽  
Signal Strength ◽  
Satellite Orbit ◽  
Satellite System ◽  
Earth Orbit ◽  
Base Line ◽  
Signal Characteristics ◽  
Better Than

An in-depth and comprehensive assessment of new observations from BDS-3 satellites is presented, with the main focus on the Carrier-to-Noise density ratio (C/N0), the quality of code and carrier phase observations for B1C and B2a signal. The signal characteristics of geosynchronous earth orbit (GEO), inclined geosynchronous satellite orbit (IGSO) and medium earth orbit (MEO) satellites of BDS-3 were grouped and compared, respectively. The evaluation results of the new B1C and B2a signals of BDS-3 were compared with the previously B1I/B2I/B3I signals and the interoperable signals of GPS, Galileo and quasi-zenith satellite system (QZSS) were compared simultaneously. As expected, the results clearly show that B1C and B2a have better signal strength and higher accuracy, including code and carrier phase observations. The C/N0 of the B2a signal is about 3 dB higher than other signals. One exception is the code observation accuracy of B3I, which value is less than 0.15 m. The carrier precision of B1C and B2a is better than that of B1I/B2I/B3I. Despite difference-in-difference (DD) observation quantity or zero-base line evaluation is adopted, while B1C is about 0.3 mm higher carrier precision than B2a. The BDS-3 MEO satellite and GPS, Galileo, and QZSS satellites have the same level of signal strength, code and phase observation accuracy at the interoperable frequency, namely 1575.42 MHz and 1176.45 MHz which are very suitable for the co-position application.

The stability of maize nuclei to restriction enzyme digestion differs with transcriptional activity

Caryologia ◽  
1993 ◽  
Vol 46 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Valeria Mirkova ◽  
Maria Ivanchenko ◽  
Lubomir Stoilov ◽  
Jordanka Zlatanova
Keyword(s):  
Restriction Enzyme ◽  
Transcriptional Activity ◽  
Enzyme Digestion ◽  
Restriction Enzyme Digestion ◽  
The Stability

OPTICAL DENSITY RATIO OF THE SUBRETINAL FLUID IN CHOROIDAL MELANOMA AND METASTASIS

Retina ◽  
2019 ◽  
Vol 39 (4) ◽  
pp. 685-691 ◽  
Author(s):  
Ari Leshno ◽  
Vicktoria Vishnevskia-Dai ◽  
Adiel Barak ◽  
Dinah Zur ◽  
Shiran Gabai ◽  
...  
Keyword(s):  
Optical Density ◽  
Density Ratio ◽  
Choroidal Melanoma ◽  
Subretinal Fluid ◽  
Optical Density Ratio

scholarly journals A novel decatenation assay for DNA topoisomerases using a singly-linked catenated substrate

BioTechniques ◽  
2020 ◽  
Vol 69 (5) ◽  
pp. 356-362
Author(s):  
Nidda F Waraich ◽  
Shruti Jain ◽  
Sean D Colloms ◽  
William Marshall Stark ◽  
Nicolas P Burton ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Enzyme Digestion ◽  
Restriction Enzyme Digestion ◽  
Kinetoplast Dna ◽  
Dna Topoisomerases ◽  
In Vitro Drug Screening ◽  
Drug Inhibition ◽  
Dna Substrate

Decatenation is a crucial in vivo reaction of DNA topoisomerases in DNA replication and is frequently used in in vitro drug screening. Usually this reaction is monitored using kinetoplast DNA as a substrate, although this assay has several limitations. Here we have engineered a substrate for Tn 3 resolvase that generates a singly-linked catenane that can readily be purified from the DNA substrate after restriction enzyme digestion and centrifugation. We show that this catenated substrate can be used with high sensitivity in topoisomerase assays and drug-inhibition assays.

scholarly journals Clinical Implications of Suspended Scattering Particles in Motion Observed by Optical Coherence Tomography Angiography

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jaemoon Ahn ◽  
Sangheon Han ◽  
So Min Ahn ◽  
Seong-Woo Kim ◽  
Jaeryung Oh
Keyword(s):  
Optical Coherence Tomography ◽  
Regression Model ◽  
Treatment Response ◽  
Optical Coherence Tomography Angiography ◽  
Density Ratio ◽  
Response To Treatment ◽  
Optical Coherence ◽  
Binary Logistic Regression Model ◽  
Hyperreflective Foci ◽  
Optical Density Ratio

AbstractThe objective of this study was to investigate the relationship between suspended scattering particles in motion (SSPiM) in optical coherence tomography angiography (OCTA) and treatment response in diabetic macular edema (DME). We retrospectively reviewed the medical records of patients diagnosed with DME who had undergone intravitreal injection. The optical density ratio (ODR) of the intraretinal cyst and the numbers of hyperreflective foci from OCT images and SSPiM from OCTA images were compared, and their association with treatment response was analyzed. Forty-five eyes from 45 patients were included in this study. Twenty-four patients were treated with anti-vascular endothelial growth factor, and 21 patients were treated with a steroid. Binary logistic regression model showed that SSPiM in OCTA images was associated with hyperreflective foci numbers (P = 0.038) and mean ODR of the intraretinal cyst (P = 0.006). Linear regression model showed that SSPiM in the inner nuclear layer was related to treatment response (P = 0.006). SSPiM on OCTA images is related to the poor structural response to treatment in DME.

Evidence for selection for the tyrosine-86 allele of the pfmdr 1 gene of Plasmodium falciparum by chloroquine and amodiaquine

Parasitology ◽  
1997 ◽  
Vol 114 (3) ◽  
pp. 205-211 ◽  
Author(s):  
M. T. DURAISINGH ◽  
C. J. DRAKELEY ◽  
O. MULLER ◽  
R. BAILEY ◽  
G. SNOUNOU ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Plasmodium Falciparum ◽  
Genetic Basis ◽  
Drug Trial ◽  
Enzyme Digestion ◽  
Restriction Enzyme Digestion ◽  
Blood Samples ◽  
Allelic Status ◽  
Group A ◽  
Selection For

The 4-aminoquinolines chloroquine (CQ) and amodiaquine (AM) were used to treat Gambian children with uncomplicated falciparum malaria in a randomized drug trial. Blood samples were taken immediately before treatment (day 0), and at day 7 and day 28 after treatment. Samples from those parasitologically positive at day 7 following treatment (‘early positives’) and those positive at day 28 but negative at day 7 (‘late positives’) have been studied by PCR followed by restriction enzyme digestion to determine the allelic status of the pfmdr 1 locus at the codon-86 position (asparagine or tyrosine), previously associated with resistance to CQ. A significantly higher prevalence of the tyr-86 allele was observed in samples taken immediately before treatment (day 0) in the early positives group when compared with the late positives group. This suggests the tyr-86 allele contributes to drug resistance in the early positives group. This association remained significant for both CQ and AM groups, implying a common genetic basis of resistance. Predominance of the allele at day 7 is consistent with a strong selection in the first week following treatment. In the late positives group, a significantly higher prevalence of the tyr-86 allele was observed in the samples at day 28 when compared with those at day 0, suggestive of selection during the period day 7 to day 28. Differences were observed in the extent of this selection in the CQ and AM groups. The samples were genotyped at 3 unlinked polymorphic loci. These analyses suggested that most parasites observed at day 7 were probably recrudescences whereas most of those at day 28 were reinfections.

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