Taurine and vitamin E supplementations have minimal effects on body composition, hepatic lipids, and blood hormone and metabolite concentrations in healthy Sprague Dawley rats

The effects that chronic cocaine administration (CCA) have on craving, cocaine metabolite concentrations and cytochrome P450 3A4 isoenzyme (CYP450 3A4) activities in Sprague-Dawley rats following the administration of Salako Nutritional Supplements (SNS) were examined. Five groups of fifty rats were used to assess the effect of the SNS following CCA. Craving was analyzed for each rat using a Conditioned Place Preference protocol. Blood samples were obtained at regular intervals and used to measure cocaine plasma metabolite levels. CYP450 3A4 activity was determined in the liver. Administration of the SNS reduced craving of cocaine significantly, upon discontinuing cocaine in the rats. Blood plasma analysis showing higher benzoylecgonine equilibrium and the CYP450 3A4 levels demonstrated that the SNS possibly aided in the removal of the stored metabolites indicative of increased metabolism of cocaine, enhanced by the Supplements. Results indicate that the SNS formulation reduces craving caused by CCA by increasing the liver CYP450 3A4 activity, resulting in better plasma clearance.

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Effects of megestrol acetate and testosterone on body composition in castrated male Sprague-Dawley rats

Nutrition ◽

10.1016/s0899-9007(99)00053-2 ◽

1999 ◽

Vol 15 (6) ◽

pp. 465-473 ◽

Cited By ~ 13

Author(s):

Ellen S Engelson ◽

F.Xavier Pi-Sunyer ◽

Donald P Kotler

Keyword(s):

Body Composition ◽

Megestrol Acetate ◽

Sprague Dawley ◽

Sprague Dawley Rats

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Vitamin E Concentration in Rat Skeletal Muscle and Liver after Exercise

International Journal of Sport Nutrition ◽

10.1123/ijsn.8.2.105 ◽

1998 ◽

Vol 8 (2) ◽

pp. 105-112 ◽

Cited By ~ 6

Author(s):

Jon N. Swift ◽

James P. Kehrer ◽

K. Stephen Seiler ◽

Joseph W. Starnes

Keyword(s):

Skeletal Muscle ◽

Vitamin E ◽

High Performance ◽

Time Course ◽

Vastus Lateralis ◽

Sprague Dawley ◽

Rat Skeletal Muscle ◽

Sedentary Control ◽

Sprague Dawley Rats ◽

Response To Exercise

The purpose of this study was to determine whether submaximal exercise significantly changes the concentration of vitamin E (αToc) in rat liver and skeletal muscle and to establish a time course for the return to basal levels. Male Sprague-Dawley rats, age 8 to 10 weeks, were randomly divided into sedentary control (Con) (n = 7) and exercise n = 17) groups. Exercised animals ran 100 min on a motorized treadmill at approximately 70% VO2max for 3 consecutive days. They were then sacrificed immediately postexercise (0Post), 24 hr post (24Post), or 72 hr post (72Post). The gastrocnemius, red vastus lateralis (RV), white vastus lateralis (WV), and liver were excised and analyzed for αToc concentration by high-performance liquid chromolography utilizing electrochemical detection. We found that after 3 consecutive days of exercise, αToc was reduced in RV and WV at 0Post and 24Post but returned to control values by 72Post. Liver αToc content was not changed at OPost but was significantly reduced at 24 Post and 72 Post. No significant changes in αToc were observed in the gastrocnemius in response to exercise. The data indicate that following an exercise-related decrease, skeletal muscle vitamin E concentration requires more than 24 hr to return to the preexercise concentration, and that the replenishment process may involve redistribution of vitamin E from liver to muscle.

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Vitamin E reduces glomerulosclerosis, restores renal neuronal NOS, and suppresses oxidative stress in the 5/6 nephrectomized rat

AJP Renal Physiology ◽

10.1152/ajprenal.00260.2006 ◽

2007 ◽

Vol 292 (5) ◽

pp. F1404-F1410 ◽

Cited By ~ 61

Author(s):

You-Lin Tain ◽

Gary Freshour ◽

Anna Dikalova ◽

Kathy Griendling ◽

Chris Baylis

Keyword(s):

Oxidative Stress ◽

Nitric Oxide ◽

Vitamin E ◽

Kidney Cortex ◽

Antioxidant Vitamin ◽

No Production ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Endothelial Nitric Oxide

Chronic kidney disease is accompanied by nitric oxide (NO) deficiency and oxidative stress, which contribute to progression. We investigated whether the antioxidant vitamin E could preserve renal function and NO bioavailability and reduce oxidative stress in the 5/6th nephrectomy (NX) rat model. We studied the following three groups of male Sprague-Dawley rats: sham ( n = 6), 5/6 NX control ( n = 6), and 5/6 NX treated with vitamin E (5,000 IU/kg chow; n = 5). The 5/6 NX group showed increased severity of glomerulosclerosis vs. sham, and this was ameliorated by vitamin E therapy. Both 5/6 NX groups showed similar elevations in plasma creatinine and proteinuria and decreased 24-h creatinine clearance compared with sham. There was increased NADPH-dependent superoxide production in 5/6 NX rats vs. sham that was prevented by vitamin E. Total NO production was similarly reduced in both 5/6 NX groups. There was unchanged abundance of endothelial nitric oxide synthesis (NOS) in renal cortex and medulla and neuronal (n) NOS in medulla. However, in kidney cortex, 5/6 NX rats had lower nNOS abundance than sham, which was restored by vitamin E. An increased plasma asymmetric dimethylarginine occurred with 5/6 NX associated with decreased renal dimethylarginine dimethylaminohydrolase activity and increased type 1 protein arginine methyltransferase expression.

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Influence of Plane of Nutrition on Body Composition, Organ Size and Energy Utilization of Sprague-Dawley Rats

Journal of Nutrition ◽

10.1093/jn/116.12.2525 ◽

1986 ◽

Vol 116 (12) ◽

pp. 2525-2535 ◽

Cited By ~ 44

Author(s):

C. L. Ferrell ◽

K. J. Koong

Keyword(s):

Body Composition ◽

Organ Size ◽

Energy Utilization ◽

Sprague Dawley ◽

Sprague Dawley Rats

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Effects ofPiper nigrumextracts: Restorative perspectives of high-fat diet-induced changes on lipid profile, body composition, and hormones in Sprague–Dawley rats

Pharmaceutical Biology ◽

10.3109/13880209.2014.980585 ◽

2015 ◽

Vol 53 (9) ◽

pp. 1318-1328 ◽

Cited By ~ 12

Author(s):

BrahmaNaidu Parim ◽

Nemani Harishankar ◽

Meriga Balaji ◽

Sailaja Pothana ◽

Ramgopal Rao Sajjalaguddam

Keyword(s):

Body Composition ◽

Lipid Profile ◽

High Fat Diet ◽

Sprague Dawley ◽

High Fat ◽

Sprague Dawley Rats ◽

Induced Changes

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Effects of Dietary Carnosine and Vitamin E on Antioxidant and Oxidative Status of Rats

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.78.45.230 ◽

2008 ◽

Vol 78 (45) ◽

pp. 230-237 ◽

Cited By ~ 10

Author(s):

Wissam Ibrahim ◽

Vickie Tatumi ◽

Che-Chung Yeh ◽

Chuen Bin Hong ◽

Ching Kuang Chow

Keyword(s):

Vitamin E ◽

Antioxidant Status ◽

Conjugated Dienes ◽

Oxidative Status ◽

Tocopheryl Acetate ◽

Protein Carbonyls ◽

Dietary Vitamin ◽

Sprague Dawley ◽

Deficient Diet ◽

Sprague Dawley Rats

The purpose of this study was to determine if moderate levels of carnosine supplement, alone or in combination with vitamin E, enhance antioxidant status and/or provide protection against oxidative stress. Fiftyfour one-month-old male Sprague-Dawley rats were fed a basal vitamin E-deficient diet supplemented with either 0, 200, or 1000 mg L-carnosine, and either 0, 10, or 100 IU vitamin E (as all rec-α-tocopheryl acetate) per kg diet for 15 weeks. The antioxidant and oxidative status were assessed in the skeletal muscle, liver, and blood. Dietary vitamin E, but not carnosine, increased levels of vitamin E, decreased tissue peroxidizability, prevented incidence of myodegeneration, and reduced erythrocyte hemolytic stress. The levels of conjugated dienes, protein carbonyls, ascorbic acid, and nonprotein sulfhydryls, and activities of catalase, glutathione (GSH) peroxidase, and aldehyde dehydrogenase were not significantly altered by dietary carnosine or vitamin E. The results obtained suggest that supplementation of carnosine at levels of up to 1000 mg/kg diet does not significantly affect the antioxidant and oxidative status of rats.

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A Novel Micronutrient Supplement to Mitigate the Transgenerational Effects of Paternal Obesity on Body Composition of Male Offspring (P11-138-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz048.p11-138-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Author(s):

Md Mustahsan Billah ◽

Saroj Khatiwada ◽

Virginie Lecomte ◽

Margaret Morris ◽

Chris Maloney

Keyword(s):

Body Composition ◽

Fat Mass ◽

Lean Mass ◽

Fat Deposition ◽

Sprague Dawley ◽

Transgenerational Effects ◽

Funding Sources ◽

Sprague Dawley Rats ◽

Offspring Health ◽

Paternal Obesity

Abstract Objectives Emerging evidence suggests that paternal obesity plays a significant role on offspring health. Our previous work in rodents showed that high fat diet (HFD) induced paternal obesity reduced offspring glucose tolerance. It has also been reported that paternal obesity can initiate metabolic disturbance in subsequent generations. Therefore, we designed a novel micronutrient supplement to ameliorate the transgenerational effects of paternal obesity. Methods Founder (F0) male Sprague Dawley rats (3 weeks, 12 per group) were weaned on control (CD) or HFD or diets containing micronutrient supplement (CS; HS), after which they were mated with CD fed females at 19 weeks of age. Twelve F1 offspring from each litter across the four diet groups were weaned on day 21 onto CD, generating four F1 groups. After 14 weeks on CD, they were mated with CD fed females. Male F2 siblings were fed CD or HFD from day 21. After 6 weeks on diet, they underwent EchoMRI (See attached study design). Results HFD increased adiposity (CD: 12.3%, HFD: 18.6%) in F0 which was normalized by supplementation (HS: 11.7%). Though no paternal effect on adiposity was found in F1 males across the four groups, supplementing F0 had significant impact on F2 body composition (fat mass, fat and lean percentage) without influencing body weight and lean mass after 6 weeks on diet. In F2, post weaning HFD significantly increased fat mass in rats from CD and HFD fed founders (CH vs CC P = 0.0001; HH vs HC P = 0.009) but this effect was significantly reduced by F0 supplementation irrespective of F0 diets (CSH vs CH P = 0.017; and HSH vs HH P = 0.04) [3-way ANOVA][See attached table]. Moreover, lean percentage was decreased by post weaning HFD in rats from both non-supplemented F0 diet groups but interestingly this effect was significantly reduced only in rats sired by supplemented CD fed F0 (CSH > CH; P = 0.04). Conclusions Supplementing CD fed grandfathers ameliorated the deleterious effect of HFD, decreasing fat deposition and increasing lean percentage whereas supplementing HFD fed F0 reduced only fat deposition without affecting lean mass of male grand offspring. Therefore, our designed supplement could eventually reduce the transgenerational effect of paternal obesity. Funding Sources Supported by Australian NHMRC grant to MJM and CAM; MMB is supported by Scientia PhD Scholarship, UNSW. Supporting Tables, Images and/or Graphs

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