scholarly journals A comparison of diagnostic panels in the immunohistochemical analysis of lung cancer

2019 ◽  
Vol Volume 11 ◽  
pp. 7-15
Author(s):  
Sarita Prabhakaran ◽  
Guang Xing ◽  
Ashleigh Hocking ◽  
Matthew Hussey ◽  
Douglas W. Henderson ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Immunohistochemical Analysis

scholarly journals A detailed immunohistochemical analysis of the PI3K/AKT/mTOR pathway in lung cancer: Correlation with PIK3CA, AKT1, K-RAS or PTEN mutational status and clinicopathological features

2013 ◽  
Vol 30 (2) ◽  
pp. 623-636 ◽  
Author(s):  
ELENI ANDRIANA TRIGKA ◽  
GEORGIA LEVIDOU ◽  
ANGELICA A. SAETTA ◽  
ILENIA CHATZIANDREOU ◽  
PERIKLIS TOMOS ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Immunohistochemical Analysis ◽  
Mtor Pathway ◽  
Clinicopathological Features ◽  
Mutational Status

scholarly journals Prognostic Significance of Stromal Periostin Expression in Non-Small Cell Lung Cancer

2020 ◽  
Vol 21 (19) ◽  
pp. 7025
Author(s):  
Katarzyna Ratajczak-Wielgomas ◽  
Alicja Kmiecik ◽  
Jedrzej Grzegrzołka ◽  
Aleksandra Piotrowska ◽  
Agnieszka Gomulkiewicz ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Prognostic Factor ◽  
Small Cell Lung Cancer ◽  
Mrna Expression ◽  
Cancer Cells ◽  
Cell Lung Cancer ◽  
Prognostic Significance ◽  
Immunohistochemical Analysis ◽  
Small Cell ◽  
Small Cell Lung

Background: The microenvironment of solid tumours is significant in cancer development and progression. The aim of this study was to determine periostin (POSTN) expression by cancer-associated fibroblasts (CAFs) in non-small-cell lung cancer (NSCLC), as well as to assess associations with clinicopathological factors and prognosis. Materials and Methods: Immunohistochemical analysis of POSTN expression was performed on NSCLC (N = 700) and non-malignant lung tissue (NMLT) (N = 110) using tissue microarrays. Laser capture microdissection (LCM) for isolation of stromal and cancer cells of NSCLC was employed, and subsequently, POSTN mRNA expression was detected by real-time PCR. Immunofluorescence reaction and colocalisation analysis were performed by confocal microscopy. Results: Expression of POSTN in CAFs was significantly higher in NSCLC and in the adenocarcinoma (AC) and squamous cell carcinoma (SCC) subtypes compared to NMLT. POSTN expression in CAFs increased with clinical cancer stage, grades (G) of malignancy, and lymph node involvement in NSCLC. Higher POSTN expression in CAFs was an independent prognostic factor for overall survival (OS). LCM confirmed significantly higher POSTN mRNA expression in the stromal cells (CAFs) compared to the lung cancer cells. Conclusions: POSTN produced by CAFs might be crucial for NSCLC progression and can be an independent negative prognostic factor in NSCLC.

Changes in PD-L1 expression in non-small cell lung cancer by immunohistochemical analysis.

2015 ◽  
Vol 33 (15_suppl) ◽  
pp. e22118-e22118 ◽  
Author(s):  
Shota Omori ◽  
Hirotsugu Kenmotsu ◽  
Masato Abe ◽  
Reiko Watanabe ◽  
Takashi Sugino ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Immunohistochemical Analysis ◽  
Small Cell ◽  
Small Cell Lung

scholarly journals BMI1,ALDH1A1, andCD133Transcripts Connect Epithelial-Mesenchymal Transition to Cancer Stem Cells in Lung Carcinoma

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Ana Koren ◽  
Matija Rijavec ◽  
Izidor Kern ◽  
Eva Sodja ◽  
Peter Korosec ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Mrna Expression ◽  
Whole Blood ◽  
Epithelial Mesenchymal Transition ◽  
Immunohistochemical Analysis ◽  
Underlying Mechanism ◽  
Primary Tumors ◽  
Mesenchymal Transition ◽  
Cellular Models ◽  
Nsclc Patients

Epithelial-mesenchymal transition (EMT) is the underlying mechanism of tumor invasion and metastasis. Evidences from lung cancer cellular models show EMT can trigger conversion to a cancer stem cell (CSC) phenotype. In this study, we assessed mRNA expression levels of EMT-inducing transcription factors (BMI1,TWIST1), CSC (CD133,ALDH1A1), and epithelial (EpCAM) markers in primary tumor and whole blood samples obtained from 57 patients with operable non-small-cell lung cancer (NSCLC) as well as in circulating tumor cells (CTCs) of 13 patients with metastatic disease; then possible associations between marker expressions were evaluated. In primary tumors as well as in whole blood, correlations betweenBMI1andALDH1A1and betweenBMI1andCD133mRNA expressions were identified. No correlations betweenTWIST1and CSC markers were observed.BMI1mRNA expression in tumors positively correlated withBMI1mRNA expression in blood. The immunohistochemical analysis confirmed coexpression of BMI1 and CSC markers in tumors. Gene expression profiling in CTCs revealed upregulated expression of EMT/CSC markers in CTCs. Our results suggest CSCs are present in both, tumor tissue and blood of NSCLC patients, whereas Bmi1 may play an important role in initiation and maintenance of CSCs and might be involved in the blood-borne dissemination of NSCLC.

927: Immunohistochemical analysis reveals frequent tumoural loss of ATM protein expression in lung cancer

2014 ◽  
Vol 50 ◽  
pp. S227 ◽  
Author(s):  
A.M. Weber ◽  
A.M. Devery ◽  
S.M. Bokobza ◽  
A.J. Ryan
Keyword(s):  
Lung Cancer ◽  
Protein Expression ◽  
Immunohistochemical Analysis ◽  
Atm Protein
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