scholarly journals In silico identification of candidate microRNAs and their targets in potato somatic hybrid Solanum tuberosum (+) S. pinnatisectum for late blight resistance

Plant Omics ◽  
2016 ◽  
Vol 9 (2) ◽  
pp. 159-164 ◽  
Author(s):  
Ritu Singh ◽  
◽  
Jagesh Kumar Tiwari ◽  
Shashi Rawat ◽  
Vinay Sharma ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Somatic Hybrid ◽  
In Silico ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
In Silico Identification

scholarly journals Tagging QTLs for late blight resistance and plant maturity from diploid wild relatives in a cultivated potato (Solanum tuberosum) background

2007 ◽  
Vol 115 (1) ◽  
pp. 101-112 ◽  
Author(s):  
J. Śliwka ◽  
H. Jakuczun ◽  
R. Lebecka ◽  
W. Marczewski ◽  
C. Gebhardt ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Late Blight Resistance ◽  
Wild Relatives ◽  
Blight Resistance ◽  
Cultivated Potato ◽  
Plant Maturity

Genome sequence analysis provides insights on genomic variation and late blight resistance genes in potato somatic hybrid (parents and progeny)

2021 ◽  
Vol 48 (1) ◽  
pp. 623-635
Author(s):  
Jagesh Kumar Tiwari ◽  
Shashi Rawat ◽  
Satish K. Luthra ◽  
Rasna Zinta ◽  
Sarika Sahu ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Late Blight ◽  
Genome Sequence ◽  
Resistance Genes ◽  
Somatic Hybrid ◽  
Late Blight Resistance ◽  
Genomic Variation ◽  
Blight Resistance ◽  
Genome Sequence Analysis

Introgression of late blight resistance into Solanum tuberosum

1999 ◽  
pp. 25-32 ◽  
Author(s):  
G. Ramsay ◽  
H. E. Stewart ◽  
W. De Jong ◽  
J. E. Bradshaw ◽  
G. R. Mackay
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Late Blight Resistance ◽  
Blight Resistance

Interspecific potato somatic hybrids between Solanum tuberosum and S. cardiophyllum, potential sources of late blight resistance breeding

2015 ◽  
Vol 123 (3) ◽  
pp. 579-589 ◽  
Author(s):  
Poonam Chandel ◽  
Jagesh Kumar Tiwari ◽  
Nilofer Ali ◽  
Sapna Devi ◽  
Shashi Sharma ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Somatic Hybrids ◽  
Resistance Breeding ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Potential Sources

scholarly journals Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nam Phuong Kieu ◽  
Marit Lenman ◽  
Eu Sheng Wang ◽  
Bent Larsen Petersen ◽  
Erik Andreasson
Keyword(s):  
Late Blight ◽  
Late Blight Resistance ◽  
Susceptibility Genes ◽  
Blight Resistance ◽  
R Genes ◽  
Guide Rnas ◽  
Knockout Mutants ◽  
Growth Phenotype ◽  
Allelic Deletion ◽  
S Genes

AbstractThe use of pathogen-resistant cultivars is expected to increase yield and decrease fungicide use in agriculture. However, in potato breeding, increased resistance obtained via resistance genes (R-genes) is hampered because R-gene(s) are often specific for a pathogen race and can be quickly overcome by the evolution of the pathogen. In parallel, susceptibility genes (S-genes) are important for pathogenesis, and loss of S-gene function confers increased resistance in several plants, such as rice, wheat, citrus and tomatoes. In this article, we present the mutation and screening of seven putative S-genes in potatoes, including two DMR6 potato homologues. Using a CRISPR/Cas9 system, which conferred co-expression of two guide RNAs, tetra-allelic deletion mutants were generated and resistance against late blight was assayed in the plants. Functional knockouts of StDND1, StCHL1, and DMG400000582 (StDMR6-1) generated potatoes with increased resistance against late blight. Plants mutated in StDND1 showed pleiotropic effects, whereas StDMR6-1 and StCHL1 mutated plants did not exhibit any growth phenotype, making them good candidates for further agricultural studies. Additionally, we showed that DMG401026923 (here denoted StDMR6-2) knockout mutants did not demonstrate any increased late blight resistance, but exhibited a growth phenotype, indicating that StDMR6-1 and StDMR6-2 have different functions. To the best of our knowledge, this is the first report on the mutation and screening of putative S-genes in potatoes, including two DMR6 potato homologues.

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