A Capillary Zone Electrophoresis Method for Analysis of Glycosylated Recombinant Human Follicle-Stimulating Hormone

2019 ◽  
Vol 35 (4) ◽  
pp. 55-64
Author(s):  
M.E. Sushkin ◽  
A.S. Simbirtsev
Keyword(s):  
Sodium Chloride ◽  
Capillary Zone Electrophoresis ◽  
Protein Separation ◽  
Follicle Stimulating Hormone ◽  
Background Electrolyte ◽  
Sialic Acids ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone ◽  
Stimulating Hormone

Conditions for analyzing sialylated isoforms of recombinant follicle-stimulating hormone (FSH) by the method of capillary zone electrophoresis have been selected. Follicle-stimulating hormone is a glycosylated heterodimer with the large number of sialic acids that affect its basic biological and physicochemical properties. The optimum protein separation into 6 isoforms was achieved using as a background electrolyte of a solution with pH 4.3 containing 0.01 M trycin, 0.01 sodium chloride, 7 M urea and 2.5 mM 1,4-diaminobutane. follicle-stimulating hormone isoforms, capillary zone electrophoresis, sialic acids, follicle-stimulating hormone.

scholarly journals Letrozole Determination by Capillary Zone Electrophoresis and UV Spectrophotometry Methods

2017 ◽  
Vol 63 (2) ◽  
pp. 80-86 ◽  
Author(s):  
Aura Rusu ◽  
Maria-Alexandra Sbanca ◽  
Nicoleta Todoran ◽  
Camil-Eugen Vari
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Bulk Material ◽  
Background Electrolyte ◽  
Pharmaceutical Formulation ◽  
Urine Samples ◽  
Uv Spectrophotometry ◽  
Validation Parameters ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone

Abstract Objective: Letrozole is a highly potent oral nonsteroidal aromatase inhibitor triazole derivative. The aim of this study was to quantify letrozole from bulk, pharmaceutical formulation, and spiked urine samples by developing a simple, rapid and cost effective capillary electrophoresis method. Methods: A capillary zone electrophoresis method was optimized and validated. Additionally, an UV spectrophotometry method was used for comparing results. Results:The capillary zone electrophoresis method using a 90 mM sodium tetraborate background electrolyte proved to be an efficient method for determination of letrozole in a very short time, less than 2 minutes, using 20 kV voltage, 50 mbar/2 seconds pressure and 50°C temperature as optimum parameters. Additionally, the UV spectrophotometry method proved to be simple and efficient to quantify letrozole from bulk material and pharmaceutical formulation with linearity of response between 5 to 20 μg·mL-1 concentrations. For both methods, validation parameters, including linearity, detection and quantification limits were determined. Also we proved that our electrophoretic method has potential in analyzing letrozole from biological samples, obtaining encouraging results on estimation of letrozole from spiked urine samples without any special treatment. Conclusions: To quantify letrozole from bulk material, pharmaceutical preparations, and spiked urine samples the capillary zone electrophoresis method using a tetraborate sodium background electrolyte has proven to be simple and appropriate. Also a simple UV spectrophotometric method has been developed and validated for the same purposes.

scholarly journals Development of a Capillary Electrophoresis Method for the Separation of Fluoroquinolone Derivatives in Acidic Background Electrolyte

2014 ◽  
Vol 60 (3) ◽  
pp. 109-115
Author(s):  
Aura Rusu ◽  
G. Hancu ◽  
Á. Gyéresi
Keyword(s):  
Capillary Zone Electrophoresis ◽  
High Performance ◽  
Background Electrolyte ◽  
Injection Pressure ◽  
Effective Length ◽  
Separation Performance ◽  
Electrophoretic Behavior ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone

Abstract Introduction: Antibacterial quinolones class comprises a series of synthetic antibacterial agents, following the model of nalidixic acid. Because of their common 6-fl uorosubtituent on the quinolone ring, fluroquinolones are the most potent analogues with extended spectrum of activity and great pharmacokinetic properties. The applicability of capillary zone electrophoresis for the separation of fl uoroquinolones in acidic background electrolyte has been studied, our aim being the development of a capillary zone electrophoresis method for the simultaneous separation of six fl uoroquinolones and also to optimize the analytical conditions. The six studied fl uoroquinolones were ciprofloxacin, enoxacin, enrofloxacin, moxifloxacin, ofloxacin and sarafloxacin. Material and methods: Preliminary, we studied the electrophoretic behavior of six fluoroquinolones in an acidic pH, which highlighted the possibility of developing a separation method in this area of pH. Electrophoretic parameters infl uencing separation performance were studied and optimized. Results: A fast and reliable method has been developed, using a background electrolyte containing 100 mM phosphoric acid and the following conditions: applied voltage: + 25 kV, temperature: 20°C, injection pressure 30 mbar - 5 sec, UV detection at 280 nm, capillary: 60 cm (52 cm effective length) x 50 μm, analyte concentration: 167 μg/ml. The separation of the studied fl uoroquinolones was achieved in less than 8 minutes. Conclusions: Capillary zone electrophoresis using an acidic background electrolyte proved to be an efficient tool in the separation of fluoroquinolones from different generations. Also the proposed methods are particular environment-friendly replacement and improvement of a common high performance liquid chromatography determination with rapid analysis time without using any organic solvents.

Simple and Rapid Capillary Zone Electrophoresis Method for the Detection of Coronamic Acid, a Precursor to thePseudomonas syringaePhytotoxin Coronatine

2009 ◽  
Vol 57 (22) ◽  
pp. 10518-10523 ◽  
Author(s):  
Aswathy Sreedharan ◽  
Alejandro Penaloza-Vazquez ◽  
Ma. Cristina Escober ◽  
Carol L. Bender ◽  
Patricia Rayas-Duarte
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone

scholarly journals Separation of Arsenic and Selenium Species by Capillary Zone Electrophoresis in a Coated Capillary

1999 ◽  
Vol 82 (6) ◽  
pp. 1587-1593 ◽  
Author(s):  
Natalia G Vanifatova ◽  
Boris Y Spivakov ◽  
Juergen Mattusch ◽  
Rainer Wennrich
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Sensitivity Enhancement ◽  
Selenium Species ◽  
Long Term Stability ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone ◽  
Matrix Removal

Abstract A capillary zone electrophoresis method was developed for the determination of 8 arsenic and selenium species in a polymer-coated capillary. Large-volume stacking with matrix removal was used for sensitivity enhancement. The entire analysis time was only a few minutes. The experimental sensitivity enhancement for 7 species was found to be near its theoretical value. Coating capillaries for long-term stability in alkaline buffer electrolytes was also investigated.

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