A BIOCHEMICAL STUDY OF THE EFFECT OF HIGH MOLECULAR WEIGHT HYALURONIC ACID IN MANAGEMENT OF TEMPOROMANDIBULAR JOINT OSTEOARTHRITIS IN RATS

Temporomandibular joint osteoarthritis (TMJ OA) is a low-inflammatory disorder with multifactorial etiology. The aim of this review was to present the current state of knowledge regarding the mechanisms of action and the efficacy of hyaluronic acid (HA), corticosteroids (CS) and platelet-rich plasma (PRP) in the treatment of TMJ OA.: The PubMed database was analyzed with the keywords: “(temporomandibular joint) AND ((osteoarthritis) OR (dysfunction) OR (disorders) OR (pain)) AND ((treatment) OR (arthrocentesis) OR (arthroscopy) OR (injection)) AND ((hyaluronic acid) OR (corticosteroid) OR (platelet rich plasma))”. After screening of 363 results, 16 studies were included in this review. Arthrocentesis alone effectively reduces pain and improves jaw function in patients diagnosed with TMJ OA. Additional injections of HA, either low-molecular-weight (LMW) HA or high-molecular-weight (HMW) HA, or CS at the end of the arthrocentesis do not improve the final clinical outcomes. CS present several negative effects on the articular cartilage. Results related to additional PRP injections are not consistent and are rather questionable. Further studies should be multicenter, based on a larger group of patients and should answer the question of whether other methods of TMJ OA treatment are more beneficial for the patients than simple arthrocentesis.

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Preparation and Characterization of the High Molecular Weight [3H]Hyaluronic Acid

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19922151 ◽

1992 ◽

Vol 57 (10) ◽

pp. 2151-2156 ◽

Cited By ~ 2

Author(s):

Peter Chabreček ◽

Ladislav Šoltés ◽

Hynek Hradec ◽

Jiří Filip ◽

Eduard Orviský

Keyword(s):

Molecular Weight ◽

Hyaluronic Acid ◽

High Molecular Weight ◽

Methyl Bromide ◽

High Performance ◽

Hydrogen Atoms ◽

Isolation And Characterization ◽

Labelling Procedure ◽

Enzymatic Depolymerization

Two methods for the preparation of high molecular weight [3H]hyaluronic acid were investigated. In the first one, hydrogen atoms in the molecule were replaced by tritium. This isotopic substitution was performed in aqueous solution using Pd/CaCO3 as the catalyst. In the second method, the high molecular weight hyaluronic acid was alkylated with [3H]methyl bromide in liquid ammonia at a temperature of -33.5 °C. High-performance gel permeation chromatographic separation method was used for the isolation and characterization of the high molecular weight [3H]hyaluronic acid. Molecular weight parameters for the labelled biopolymers were Mw = 128 kDa, Mw/Mn = 1.88 (first method) and Mw = 268 kDa, Mw/Mn = 1.55 (second method). The high molecular weight [3H]hyaluronic acid having Mw = 268 kDa was degraded further by specific hyaluronidase. Products of the enzymatic depolymerization were observed to be identical for both, labelled and cold biopolymer. This finding indicates that the described labelling procedure using [3H]methyl bromide does not induce any major structural rearrangements in the molecule.

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High molecular weight form of hyaluronic acid reduces neuroinflammatory response in injured sciatic nerve via the intracellular domain of CD44

Journal of Biomedical Materials Research Part B Applied Biomaterials ◽

10.1002/jbm.b.34731 ◽

2020 ◽

Vol 109 (5) ◽

pp. 673-680

Author(s):

I‐Ming Jou ◽

Tung‐Tai Wu ◽

Che‐Chia Hsu ◽

Cheng‐Chang Yang ◽

Jhy‐Shrian Huang ◽

...

Keyword(s):

Molecular Weight ◽

Hyaluronic Acid ◽

Sciatic Nerve ◽

High Molecular Weight ◽

Intracellular Domain ◽

Neuroinflammatory Response ◽

High Molecular Weight Form ◽

Molecular Weight Form

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Temporomandibular Joint Osteoarthritis: Regenerative Treatment by a Stem Cell Containing Advanced Therapy Medicinal Product (ATMP)—An In Vivo Animal Trial

International Journal of Molecular Sciences ◽

10.3390/ijms22010443 ◽

2021 ◽

Vol 22 (1) ◽

pp. 443

Author(s):

Robert Köhnke ◽

Marcus Oliver Ahlers ◽

Moritz Alexander Birkelbach ◽

Florian Ewald ◽

Michael Krueger ◽

...

Keyword(s):

Stem Cell ◽

Hyaluronic Acid ◽

Medicinal Product ◽

Temporomandibular Joint ◽

Stromal Cells ◽

Stromal Cell ◽

Advanced Therapy Medicinal Product ◽

Animal Trial ◽

Advanced Therapy ◽

Temporomandibular Joint Osteoarthritis

Temporomandibular joint osteoarthritis (TMJ-OA) is a chronic degenerative disease that is often characterized by progressive impairment of the temporomandibular functional unit. The aim of this randomized controlled animal trial was a comparative analysis regarding the chondroregenerative potency of intra-articular stem/stromal cell therapy. Four weeks after combined mechanical and biochemical osteoarthritis induction in 28 rabbits, therapy was initiated by a single intra-articular injection, randomized into the following groups: Group 1: AB Serum (ABS); Group 2: Hyaluronic acid (HA); Group 3: Mesenchymal stromal cells (STx.); Group 4: Mesenchymal stromal cells in hyaluronic acid (HA + STx.). After another 4 weeks, the animals were euthanized, followed by histological examination of the removed joints. The histological analysis showed a significant increase in cartilage thickness in the stromal cell treated groups (HA + STx. vs. ABS, p = 0.028; HA + ST.x vs. HA, p = 0.042; STx. vs. ABS, p = 0.036). Scanning electron microscopy detected a similar heterogeneity of mineralization and tissue porosity in the subchondral zone in all groups. The single intra-articular injection of a stem cell containing, GMP-compliant advanced therapy medicinal product for the treatment of iatrogen induced osteoarthritis of the temporomandibular joint shows a chondroregenerative effect.

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