EFFECT OF HONEYBEE VENOM (APIS MELLIFERA) ON RESPIRATORY FUNCTIONS OF HYPERCHOLESTEROLEMIC MALE ALBINO RATS

The objective of this study was to determine the clinico-therapeutic effect of worker honeybee venom in sows with oligogalactic syndrome postpartum. Comparison between bee venom- and drug-treated groups was our main concern in the present study. Sows after parturition were assigned to bee venom- and drug-treated groups, respectively. In the bee venom-treated group, 22 sows were bee-acupunctured once a day for 3 consecutive days. Honeybees (Apis mellifera L.) for bee acupuncture were about 15 days old after metamorphosis. Live bees were used to sting the acupoints known as Yang-ming (ST-18, 1.5 cm lateral to the base of the last two pairs of teats) and Jiao-chao (GV-1, at the indentation between the base of tail and the anus). In the drug-treated group, 20 sows were intramuscularly injected with a standard dose of penicillin G (400,000 IU/head) once a day for 3 consecutive days. On post-treatment day 4, 85.0% of the drug-treated group and 90.9% of the bee venom-treated group recovered from oligogalactic syndrome postpartum. The result suggested that apitherapy using worker honeybee is an effective treatment for sows with oligogalactic syndrome postpartum.

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IgE cross-reactivity of phospholipase A2 and hyaluronidase of Apis dorsata (Giant Asian Honeybee) and Apis mellifera (Western Honeybee) venom: Possible use of A. mellifera venom for diagnosis of patients allergic to A. dorsata venom

Toxicon ◽

10.1016/j.toxicon.2017.07.015 ◽

2017 ◽

Vol 137 ◽

pp. 27-35 ◽

Cited By ~ 4

Author(s):

Peshala Gunasekara ◽

S.M. Handunnetti ◽

Sunil Premawansa ◽

E.W.R.A. Witharana ◽

W.M.D.K. Dasanayake ◽

...

Keyword(s):

Apis Mellifera ◽

Phospholipase A2 ◽

Cross Reactivity ◽

Apis Dorsata ◽

Honeybee Venom ◽

Asian Honeybee

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Effects of renovascular hypertension on rat adrenal zona glomerulosa

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100083783 ◽

1978 ◽

Vol 36 (2) ◽

pp. 582-583

Author(s):

G. Mazzocchi ◽

P. Rebuffat ◽

C. Robba ◽

P. Vassanelli ◽

G. G. Nussdorfer

Keyword(s):

Renovascular Hypertension ◽

Left Kidney ◽

Renin Angiotensin System ◽

Plasma Renin ◽

Zona Glomerulosa ◽

Ultrastructural Changes ◽

Albino Rats ◽

Left Renal Artery ◽

Angiotensin System ◽

And Control

It is well known that the rat adrenal zona glomerulosa steroidogenic activity is controlled by the renin-angiotensin system. The ultrastructural changes in the rat zona glomerulosa cells induced by renovascular hypertension were described previously, but as far as we are aware no correlated biochemical and morphometric investigations were performed.Twenty adult male albino rats were divided into 2 experimental groups. One group was subjected to restriction of blood flow to the left kidney by the application of a silver clip about the left renal artery. The other group was sham-operated and served as a control. Renovascular hypertension developed in about 10 days: sistolic blood pressure averaged 165 ± 6. 4 mmHg, whereas it was about 110 ± 3. 8 mmHg in the control animals. The hypertensive and control rats were sacrificed 20 days after the operation. The blood was collected and plasma renin activity was determined by radioimmunological methods. The aldosterone concentration was radioimmunologically assayed both in the plasma and in the homogenate of the left capsular adrenal gland.

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Further Studies on the Ultrastructure of Harderian Gland in Adult Rats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051451 ◽

1974 ◽

Vol 32 ◽

pp. 288-289

Author(s):

Alfredo Feria-Velasco ◽

Guadalupe Tapia-Arizmendi

Keyword(s):

Fine Structure ◽

Domestic Fowl ◽

Animal Species ◽

Acinar Cells ◽

Harderian Gland ◽

Myoepithelial Cells ◽

The Other ◽

Albino Rats ◽

Adult Rats ◽

Cell Components

The fine structure of the Harderian gland has been described in some animal species (hamster, rabbit, mouse, domestic fowl and albino rats). There are only two reports in the literature dealing on the ultrastructure of rat Harderian gland in adult animals. In one of them the author describes the myoepithelial cells in methacrylate-embbeded tissue, and the other deals with the maturation of the acinar cells and the formation of the secretory droplets. The aim of the present work is to analize the relationships among the acinar cell components and to describe the two types of cells located at the perifery of the acini.

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Cell Fine Structure as Revealed in Dessicator-Dried Resinless Sections

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099532 ◽

1981 ◽

Vol 39 ◽

pp. 622-623

Author(s):

R. P. Becker ◽

J. J. Wolosewick ◽

J. Ross-Stanton

Keyword(s):

Fine Structure ◽

Tannic Acid ◽

Three Dimensional ◽

Albino Rats ◽

Cell Organelles ◽

Vascular Perfusion ◽

Thin Sections ◽

Carbon Coated ◽

Embedding Medium ◽

Polyethylene Glycol 6000

Methodology has been introduced recently which allows transmission and scanning electron microscopy of cell fine structure in semi-thin sections unencumbered by an embedding medium. Images obtained from these “resinless” sections show a three-dimensional lattice of microtrabeculfee contiguous with cytoskeletal structures and membrane-bounded cell organelles. Visualization of these structures, especially of the matiiDra-nous components, can be facilitated by employing tannic acid in the fixation step and dessicator drying, as reported here.Albino rats were fixed by vascular perfusion with 2% glutaraldehyde or 1.5% depolymerized paraformaldehyde plus 2.5% glutaraldehyde in 0.1M sodium cacodylate (pH 7.4). Tissues were removed and minced in the fixative and stored overnight in fixative containing 4% tannic acid. The tissues were rinsed in buffer (0.2M cacodylate), exposed to 1% buffered osmium tetroxide, dehydrated in ethyl alcohol, and embedded in pure polyethylene glycol-6000 (PEG). Sections were cut on glass knives with a Sorvall MT-1 microtome and mounted onto poly-L-lysine, formvar-carbon coated grids while submerged in a solution of 95% ethanol containing 5% PEG.

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Differentiation Processes of the Ocellar Retina of the Honeybee (Apis Mellifera L.)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100159692 ◽

1990 ◽

Vol 48 (3) ◽

pp. 430-431

Author(s):

Maria Anna Pabst

Keyword(s):

Apis Mellifera ◽

Distal Part ◽

Cell Layer ◽

Single Layer ◽

Photoreceptor Cells ◽

Distal Segment ◽

Compound Eyes ◽

Thin Sections ◽

Ultrastructural Studies ◽

Adult Worker

In addition to the compound eyes, honeybees have three dorsal ocelli on the vertex of the head. Each ocellus has about 800 elongated photoreceptor cells. They are paired and the distal segment of each pair bears densely packed microvilli forming together a platelike fused rhabdom. Beneath a common cuticular lens a single layer of corneagenous cells is present.Ultrastructural studies were made of the retina of praepupae, different pupal stages and adult worker bees by thin sections and freeze-etch preparations. In praepupae the ocellar anlage consists of a conical group of epidermal cells that differentiate to photoreceptor cells, glial cells and corneagenous cells. Some photoreceptor cells are already paired and show disarrayed microvilli with circularly ordered filaments inside. In ocelli of 2-day-old pupae, when a retinogenous and a lentinogenous cell layer can be clearly distinguished, cell membranes of the distal part of two photoreceptor cells begin to interdigitate with each other and so start to form the definitive microvilli. At the beginning the microvilli often occupy the whole width of the developing rhabdom (Fig. 1).

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