Morphology of immatures of the thelytokous ant, Monomorium triviale Wheeler (Formicidae: Myrmicinae: Solenopsidini) with descriptions of the larval caste dimorphism

The ant genus Monomorium is one of the most species-rich but taxonomically problematic groups in the hyperdiverse subfamily Myrmicinae. An East Asian species, M. triviale Wheeler, W. M., produces both reproductive queens and sterile workers via obligate thelytokous parthenogenesis. Here, we describe the immature forms of M. triviale based on light and scanning electron microscopy observations, with a note on the striking caste dimorphism in the last larval instar. The last-instar queen larvae were easily recognized by their large size, "aphaenogastoroid" body shape, and rows of doorknob-like tubercles on the lateral and dorsal body surface. This type of queen-specific structure has not been found in ants in general, let alone congeneric species found in Japan. In stark contrast to the queen larvae, worker larvae showed a "pheidoroid" body shape and a body surface similar to other ants. The worker larvae were estimated to have three instars, consistent with previously described congeners. The pupae of both castes also had no cocoon, a characteristic commonly described in other Myrmicinae species. In total, the developmental period from egg to adult worker averaged 59 days under 25℃. We discuss possible functions of the tubercles of queen larvae based on previous studies.

INNOCENCE AT THREAT: A STUDY OF CHILD LABOUR IN INDIA

In this article I have found out that, how a child labour is the exclusionary form of social practice in our society, for which the child is too young to deal with such exploitation intelligently. I revealed how their innocence is in threat with the presence of various forms exploitation or human rights violation due to child labour.Besides this I also revealed about the presence of lack of awareness in the society about the child rights which allows them to employ the poor child as they find them as cheap labourand hence they can be paid half of what an adult worker would need to be paid.

Relationing of Labellum and Glossae Biomorphological Characteristics of Honeybee Workers as Breeding Tool on Honey Collection Potential of Apis Mellifera L. Honeybee Colonies

This study focused on the correlation of honey collection Potential and the length and width of labellum and glossae in worker honey bees (Apis mellifera Ligustica). Sixty honeybee A. mellifera L. colonies were selected, among these 60 colonies, 3 worker bees were sampled from each colony total numbers of samples collected were 180 adult worker foraging bees. Fifteen colonies for each group were used to check the correlation of honey production with length of labellum, width of labellum, length of glossae and the width of glossae respectively. These worker bees were bought to the laboratory frozen, boiled, dissected and mounted on the slides. Measurements of the labellum length, labellum width, glossae length and glossae width were taken by the stereomicroscope with ocular micrometer at 0.8X magnification. Correlation values for the honey collectionand length and width of labellum and glossae were high and positive. These Results support the perception that worker bees with larger labellum and glossae have more ability for honey collection potential. It is concluded that Biomorphological characters of labellum and glossae are significantly correlated with the honey collection potential in A. mellifera L.

Relationing of Labellum and Glossae Biomorphological characteristics of honeybee workers as breeding tool on honey collection potential of Apis mellifera L. honeybee colonies

This study focused on the correlation of honey collection Potential and the length and width of labellum and glossae in worker honey bees ( Apis mellifera Ligustica). Sixty honeybee A. mellifera L. colonies were selected, among these 60 colonies, 3 worker bees were sampled from each colony total numbers of samples collected were 180 adult worker foraging bees. Fifteen colonies for each group were used to check the correlation of honey production with length of labellum, width of labellum, length of glossae and the width of glossae respectively. These worker bees were bought to the laboratory frozen, boiled, dissected and mounted on the slides. Measurements of the labellum length, labellum width, glossae length and glossae width were taken by the stereomicroscope with ocular micrometer at 0.8X magnification. Correlation values for the honey collectionand length and width of labellum and glossae were high and positive. These Results support the perception that worker bees with larger labellum and glossae have more ability for honey collection Potential. It is concluded that Biomorphological characters of labellum and glossae are significantly correlated with the honeycollection Potential in A. mellifera L.

Identification of Mesophylic Bacterial Flora in Deceased Worker Adults of Apis mellifera caucasia (Pollmann, 1889)

Apis mellifera, widely farmed around the world, is the most economically important species within the genus Apis. While the microbiota of live honey bees have been extensively examined, bacteria found in deceased honey bees (which might indicate infection or opportunistic pathogens) is in contrast poorly studied. Therefore, we decided to investigate the mesophilic bacterial flora of dead honey bees. So, in September 2013, dead adult worker honey bees were collected from 12 different cities, most of which were in the border provinces of Turkey. We identified bacterial isolates at the species level by using different morphological, biochemical, physical and molecular methods, in conjunction with molecular phylogenetic analysis. We constructed phylogenetic trees for isolated bacteria with the MEGA 6.0 program and neighbor-joining trees were reconstructed based on 16S rDNA gene sequences. The phylogenetic trees indicated that isolates DE003, DE007, DE011, DE001, DE019 and DE016, DE029 could be new members of the genera Erwinia, Acidovorax, Hydrogenophaga and Bacillus genus, respectively. In the bioassay study results, we observed that DE019 Hydrogenophaga sp. (64.7%) and DE004 Klebsiella grimontii (73.3%) had lethal effects on the honey bees. The other mortalities ranged from 10% to 25% (p>0.05), and according to a One-Way ANOVA analysis DE004 and DE019 significantly affect the A. mellifera caucasia in adult worker honey bees. This study is the first report of Hydrogenophaga as honey bee pathogen.

Government strategies for supporting the adult worker model in European countries: mixed implications for defamilisation

PurposeThis article explores the link between defamilisation studies and studies of the adult worker model and discusses the mixed implications that government strategies for supporting the adult worker model have for defamilisation. The adult worker model emphasises that all adult men and women ought to engage in formal employment; defamilisation studies stress the importance of enhancing women's chances of choosing (not) to perform important family roles such as the receiver of financial support and the care provider.Design/methodology/approachTwo new strategies (“condition building” and “rewarding/penalising”) for promoting the adult worker model are identified based on literature review; their empirical significance is explored through an examination of comparative data concerning early childhood education and care policies (ECEC) and reforms in pension age in 14 countries.FindingsThe evidence shows that promoting the adult worker model does not necessarily benefit all women. While the 14 countries provide ECEC to varying extents, the increase in pension age in most countries shows that governments adopt a “rewarding/penalising” strategy for promoting the adult worker model by allocating major welfare based on people's labour force participation. These pension reforms may generate a negative impact on women's chances of attaining financial autonomy.Originality/valueThis study presents two new strategies for promoting the adult worker model and shows the empirical significance of these strategies based on comparative data. It also highlights the importance of searching for alternative concepts, namely economic defamilisation, for guiding pension reforms.

Regulation of dietary intake of protein and lipid by nurse-age adult worker honeybees

Essential macronutrients are critical to the fitness and survival of animals. Many studies have shown that animals regulate the amount of protein and carbohydrate they eat for optimal performance. Regulation of dietary fat is important but less often studied. Honeybees collect and consume floral pollen to obtain protein and fat but how they achieve the optimal balance of these two macronutrients is presently unknown. Here, using chemically defined diets composed of essential amino acids and lipids (lecithin), we show that adult worker honeybees actively regulate their intake of lipids around optimal values relative to protein in diet. We found that broodless, nurse-age worker honeybees consume foods to achieve a ratio between 1:2 and 1:3 (essential amino acids:lipid) or ∼1.25:1 protein:fat. Bees fed diets relatively high in fat gained abdominal fat and had enlarged hypopharyngeal glands. In most cases, eating diets high in fat did not result in increased mortality. Importantly, we also discovered that the total quantity of food the bees ate increased when they were given a choice of two diets relatively high in fat, implying that dietary fat influences bee nutritional state in a way that in turn, influences behaviour. We speculate that dietary fat plays a critical role in maintaining workers in the nurse-like behavioural state independently of the influence of queen pheromone.

Bacterial Composition, Community Structure, and Diversity in Apis nigrocincta Gut

Understanding the honeybee gut bacteria is an essential aspect as honeybees are the primary pollinators of many crops. In this study, the honeybee-associated gut bacteria were investigated by targeting the V3-V4 region of 16S rRNA genes using the Illumina MiSeq. The adult worker was captured in an urban area in a dense settlement. In total, 83,018 reads were obtained, revealing six phyla from 749 bacterial operational taxonomic units (OTUs). The gut was dominated by Proteobacteria (58% of the total reads, including Enterobacteriaceae 28.2%, Erwinia 6.43%, and Klebsiella 4.90%), Firmicutes (29% of the total reads, including Lactococcus garvieae 13.45%, Lactobacillus spp. 8.19%, and Enterococcus spp. 4.47%), and Actinobacteria (8% of the total reads, including Bifidobacterium spp. 7.96%). Many of these bacteria belong to the group of lactic acid bacteria (LAB), which was claimed to be composed of beneficial bacteria involved in maintaining a healthy host. The honeybee was identified as Apis nigrocincta based on an identity BLAST search of its COI region. This study is the first report on the gut microbial community structure and composition of A. nigrocincta from Indonesia.