Further Studies on the Ultrastructure of Harderian Gland in Adult Rats

1974 ◽  
Vol 32 ◽  
pp. 288-289
Author(s):  
Alfredo Feria-Velasco ◽  
Guadalupe Tapia-Arizmendi
Keyword(s):  
Fine Structure ◽  
Domestic Fowl ◽  
Animal Species ◽  
Acinar Cells ◽  
Harderian Gland ◽  
Myoepithelial Cells ◽  
The Other ◽  
Albino Rats ◽  
Adult Rats ◽  
Cell Components

The fine structure of the Harderian gland has been described in some animal species (hamster, rabbit, mouse, domestic fowl and albino rats). There are only two reports in the literature dealing on the ultrastructure of rat Harderian gland in adult animals. In one of them the author describes the myoepithelial cells in methacrylate-embbeded tissue, and the other deals with the maturation of the acinar cells and the formation of the secretory droplets. The aim of the present work is to analize the relationships among the acinar cell components and to describe the two types of cells located at the perifery of the acini.

Ultrastructure and Staining of Developing Elastic Tissue

1971 ◽  
Vol 29 ◽  
pp. 244-245
Author(s):  
E. N. Albert
Keyword(s):  
Fine Structure ◽  
Phosphotungstic Acid ◽  
Staining Method ◽  
Rat Aorta ◽  
Uranyl Acetate ◽  
The Other ◽  
Elastic Fibers ◽  
Elastic Tissue ◽  
Lead Citrate ◽  
New Born

Silver tetraphenylporphine sulfonate (Ag-TPPS) was synthesized in this laboratory and used as an electron dense stain for elastic tissue (Fig 1). The procedures for the synthesis of tetraphenylporphine sulfonate and the staining method for mature elastic tissue have been described previously.The fine structure of developing elastic tissue was observed in fetal and new born rat aorta using tetraphenylporphine sulfonate, phosphotungstic acid, uranyl acetate and lead citrate. The newly forming elastica consisted of two morphologically distinct components. These were a central amorphous and a peripheral fibrous. The ratio of the central amorphous and the peripheral fibrillar portion changed in favor of the former with increasing age.It was also observed that the staining properties of the two components were entirely different. The peripheral fibrous component stained with uranyl acetate and/or lead citrate while the central amorphous portion demonstrated no affinity for these stains. On the other hand, the central amorphous portion of developing elastic fibers stained vigorously with silver tetraphenylporphine sulfonate, while the fibrillar part did not (compare figs 2, 3, 4). Based upon the above observations it is proposed that developing elastica consists of two components that are morphologically and chemically different.

Cell Fine Structure as Revealed in Dessicator-Dried Resinless Sections

1981 ◽  
Vol 39 ◽  
pp. 622-623
Author(s):  
R. P. Becker ◽  
J. J. Wolosewick ◽  
J. Ross-Stanton
Keyword(s):  
Fine Structure ◽  
Tannic Acid ◽  
Three Dimensional ◽  
Albino Rats ◽  
Cell Organelles ◽  
Vascular Perfusion ◽  
Thin Sections ◽  
Carbon Coated ◽  
Embedding Medium ◽  
Polyethylene Glycol 6000

Methodology has been introduced recently which allows transmission and scanning electron microscopy of cell fine structure in semi-thin sections unencumbered by an embedding medium. Images obtained from these “resinless” sections show a three-dimensional lattice of microtrabeculfee contiguous with cytoskeletal structures and membrane-bounded cell organelles. Visualization of these structures, especially of the matiiDra-nous components, can be facilitated by employing tannic acid in the fixation step and dessicator drying, as reported here.Albino rats were fixed by vascular perfusion with 2% glutaraldehyde or 1.5% depolymerized paraformaldehyde plus 2.5% glutaraldehyde in 0.1M sodium cacodylate (pH 7.4). Tissues were removed and minced in the fixative and stored overnight in fixative containing 4% tannic acid. The tissues were rinsed in buffer (0.2M cacodylate), exposed to 1% buffered osmium tetroxide, dehydrated in ethyl alcohol, and embedded in pure polyethylene glycol-6000 (PEG). Sections were cut on glass knives with a Sorvall MT-1 microtome and mounted onto poly-L-lysine, formvar-carbon coated grids while submerged in a solution of 95% ethanol containing 5% PEG.

The Impact of L-Carnitine and Coenzyme Q10 as Protection Against Busulfan-Oxidative Stress in the Liver of Adult Rats

2020 ◽  
Vol 10 (5) ◽  
pp. 578-586
Author(s):  
Areeg M. Abdelrazek ◽  
Shimaa A. Haredy
Keyword(s):  
Oxidative Stress ◽  
Coenzyme Q10 ◽  
Protective Role ◽  
Albino Rats ◽  
Distilled Water ◽  
Negative Effects ◽  
Adult Rats ◽  
Stress Damage ◽  
The Impact ◽  
Liver Tissues

Background: Busulfan (Bu) is an anticancer drug with a variety of adverse effects for cancer patients. Oxidative stress has been considered as a common pathological mechanism and it has a key role in the initiation and progression of liver injury by Bu. Aim: The study aimed to evaluate the antioxidant impact of L-Carnitine and Coenzyme Q10 and their protective role against oxidative stress damage in liver tissues. Methods and Material: Thirty-six albino rats were divided equally into six groups. G1 (con), received I.P. injection of DMSO plus 1 ml of distilled water daily by oral gavages; G2 (Bu), received I.P. injection of Bu plus 1 ml of the distilled water daily; G3 (L-Car), received 1 ml of L-Car orally; G4 (Bu + L-Car) received I.P. injection of Bu plus 1 ml of L-Car, G5 (CoQ10) 1 ml of CoQ10 daily; and G6 (Bu + CoQ10) received I.P. injection of Bu plus 1 ml of CoQ10 daily. Results: The recent data showed that Bu induced significant (P<0.05) elevation in serum ALT, AST, liver GSSG, NO, MDA and 8-OHDG, while showing significant (P<0.05) decrease in liver GSH and ATP. On the other hand, L-Carnitine and Coenzyme Q10 ameliorated the negative effects prompted by Bu. Immunohistochemical expression of caspase-3 in liver tissues reported pathological alterations in Bu group while also showed significant recovery in L-Car more than CoQ10. Conclusion: L-Car, as well as CoQ10, can enhance the hepatotoxic effects of Bu by promoting energy production in oxidative phosphorylation process and by scavenging the free radicals.

scholarly journals The fractionation of nuclei from mammalian cells by zonal centrifugation

1968 ◽  
Vol 109 (1) ◽  
pp. 127-135 ◽  
Author(s):  
I R Johnston ◽  
A P Mathias ◽  
F. Pennington ◽  
D. Ridge
Keyword(s):  
Rat Liver ◽  
Mammalian Cells ◽  
Mouse Liver ◽  
The Other ◽  
Slow Speed ◽  
Adult Rats ◽  
Sucrose Solutions ◽  
Liver Nuclei ◽  
Effect Of Age ◽  
Zonal Rotor

1. Purified liver nuclei from adult rats separate into two main zones when centrifuged in the slow-speed zonal rotor. One zone contains diploid nuclei, the other tetraploid. 2. The effect of age on the pattern of rat liver ploidy was examined. Tetraploid nuclei are virtually absent from young animals. They increase in proportion steadily with age. Partial hepatectomy disturbs the pattern of ploidy. 3. The zonal centrifuge permits the separation of diploid, tetraploid, octaploid and hexadecaploid nuclei from mouse liver. 4. Rat liver nuclei are isopycnic with sucrose solutions of density 1·35 at 5°.

scholarly journals Interferential comparison of the red and other radiations emitted by a new cadmium lamp and the michelson lamp

1933 ◽  
Vol 139 (837) ◽  
pp. 202-218 ◽  
Keyword(s):  
Fine Structure ◽  
High Temperature ◽  
Wave Length ◽  
The Other ◽  
Reference Standard ◽  
Definite Evidence ◽  
Electro Magnetic ◽  
The Many ◽  
Red Radiation ◽  
Red Line

It is now generally recognised that future definitions of the units of length will probably be based on the length of a wave of visible light. At present the wave-length of the red radiation of cadmium serves as the basis of all measurements of the lengths of electro-magnetic waves which are perceptible by optical means, and provisional sanction has been given to measurements of length on the same basis, as an alternative to direct reference to the metre. Whether the cadmium red radiation provides the best reference standard for all measurements of length has not yet been definitely established. Two international committees, one representing spectroscopists and the other metrologists, have sanctioned standard specifications for cadmium lamps of the Michelson type from which the red radiation may be produced. The two specifications differ from one another in certain details, but both are subject to the same objections. These objections are directed partly against the high temperature at which it is necessary to run the lamp and partly against the high voltage required to excite the radiation. Therefore, such hyperfine structure and asymmetry as may be present in the red line of cadmium is likely to be masked in the Michelson lamp by a combination of two phenomena —the enhanced Doppler effect due to the high temperature of the radiating cadmium atoms, and the effect of the moderately high intensity of the electric field. Were this not so, it might be somewhat surprising that no definite evidence of fine structure or asymmetry had so far been observed in the red line from the Michelson lamp, notwithstanding the many careful examinations, with the aid of the most sensitive interferometers, to which this line has been subjected, in view of its importance as the reference standard for all other wave-lengths. Recently Nagaoka and Sugiura have recorded that they have observed slight evidences of structure in the red radiation when excited under special conditions in which great precautions were taken to ensure extreme sharpness of the line. It is believed, however, that no subsequent confirmation of this effect has yet been published.

Histological Study On Using Ileal Submucosa In Repairing Urinary Bladder Defect In Adult Albino Rat

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Eman Gomaa El Saeed ◽  
Manal H Moussa ◽  
Gehad A Hammouda ◽  
Sahar M. M Omar
Keyword(s):  
Urinary Bladder ◽  
Histological Study ◽  
Squamous Metaplasia ◽  
Muscle Layer ◽  
Control Group ◽  
Albino Rats ◽  
Adult Rats ◽  
Group I ◽  
Significant Difference ◽  
Graft Area

Abstract Background Repairing urinary bladder (UB) defect by enterocystoplasty remains the gold standard surgical bladder reconstruction procedure to increase the capacity and compliance of dysfunctional bladders. However, many complications were recorded. Aim of the work This work aimed to compare the consequences of reconstruction of urinary bladder defect using untreated small intestinal submucosal (SIS) matrix versus seeded and unseeded decellularized SIS matrix. Material and Methods Fifty female albino rats were used in this study. The animals were divided into three groups: Group I (Control) included ten adult rats from which ileal tissue was obtained. Group II included ten adult rats in which their UB defect was repaired by untreated cellular SIS. Group III included twenty adult rats that were subdivided into two subgroups, 10 rats each; Subgroup IIIA where rats had their UB defect repaired by acellular SIS and subgroup IIIb where rats had their UB defect repaired by acellular SIS seeded with adipose mesenchymal stem cells (AMSCs).Ten young rats were used for preparation of AMSCs. Morphometric and statistical analysis were also performed. Results In rats where UB defect was repaired by untreated cellular SIS, the graft area showed loss of epithelial polarity, presence of intraepithelial cysts and occasional extension of urothelium to the outer surface forming fistula. There were areas of metaplasia with the appearance PAS positive cells. In the lamina propria, there was areas of lymphocytic infiltration together with significant increase in the collagen fiber deposition (p &lt; 0.05). There was a significant decrease thickness of muscle layer as compared to control (p &lt; 0.05). In rats where UB defect was repaired by acellular SIS, urothelium in the graft area showed occasional squamous metaplasia and often the urothelium extended to the deeper layers forming Brunn's nest. There was minimal muscle regeneration in the graft area. However, in rats where UB defect was repaired by acellular SIS seeded with AMSCs, the urothelium in the graft area was nearly similar to control group with uniform urothelium thickness, minimal collagen fibers deposition and thick muscle layer that showed no significant difference from the control (p &gt; 0.05). Conclusion Acellular SIS seeded with AMSCs showed better results compared to non-seeded and cellular SIS in reconstructing urinary bladder defects.

Chronic effect of exercise on liver cholesterol of normal and hypercholesteremic rats

1963 ◽  
Vol 205 (3) ◽  
pp. 453-456 ◽  
Author(s):  
Philip D. Gollnick
Keyword(s):  
Exercise Program ◽  
Normal Diet ◽  
The Other ◽  
Liver Cholesterol ◽  
Albino Rats ◽  
Cholesterol Diet ◽  
Trained Group ◽  
Sprague Dawley ◽  
Cholesterol Accumulation ◽  
Body Weights

Two groups of male albino rats of the Sprague-Dawley strain, with average initial body weights of about 265 g, were trained for 22 weeks on an exercise program of swimming one-half hour daily in water at 35 C. One trained group was fed a normal diet containing 18% casein. The other trained group received an isocaloric diet containing 1% cholesterol which was designed to produce hypercholesteremia. Two nonexercised groups, one fed the normal and the other the 1% cholesterol diet, served as controls. The adrenals and heart ventricles of both trained groups were larger than their respective controls. Exercise had no hypocholesteremic effect on the sera of either trained group. Fat and cholesterol accumulation in the livers of rats fed the 1% cholesterol diet were not affected by training, but training significantly lowered the fat and cholesterol of the livers of the normal rats.

Actions of peptides isolated from amphibian skin on pancreatic acinar cells.

1978 ◽  
Vol 235 (2) ◽  
pp. E112 ◽  
Author(s):  
R J May ◽  
T P Conlon ◽  
V Erspamer ◽  
J D Gardner
Keyword(s):  
Cell Function ◽  
Acinar Cells ◽  
The Other ◽  
Pancreatic Acinar Cells ◽  
Amphibian Skin ◽  
Chemical Structures ◽  
Cholinergic Agents ◽  
Muscarinic Cholinergic ◽  
45Ca Release ◽  
Initial Uptake

In dispersed acinar cells prepared from guinea pig pancreas, peptides isolated from amphibian skin (caerulein, bombesin, litorin, and physalaemin) as well as eledoisin, a peptide isolated from the posterior salivary gland of a Mediterranean octopod, increased outflux of 45Ca, release of bound 45Ca, accumulation of cyclic GMP, and release of amylase. In addition, bombesin, litorin, physalaemin, and eledoisin each increased the initial uptake of 45Ca by dispersed acinar cells, whereas C-terminal octapeptide of porcine cholecystokinin (CCK-OP) and carbamylcholine did not increase the initial uptake of 45Ca but, rather, abolished the increase caused by the other agents. None of the actions of these amphibian peptides was altered by concentrations of atropine sufficient to abolish the effects of muscarinic cholinergic agents. None of the amphibian peptides altered cellular cyclic AMP or the increase caused by secretin or porcine vasoactive intestinal peptide (VIP). Acinar cells preincubated with 45Ca plus bombesin showed the same rate of release of 45Ca as did control cells and this rate was not altered by adding bombesin but was increased fivefold by adding CCK-OP. In terms of their chemical structures as well as the potency and efficacy with which they alter acinar cell function, the amphibian peptides plus CCK-OP can be grouped into three pairs: caerulein with CCK-OP, bombesin with litorin, and physalaemin with eledoisin.

scholarly journals Fine Structure and Staining Behaviour of Heterochromatic Segments in Two Plants

1974 ◽  
Vol 14 (3) ◽  
pp. 505-521
Author(s):  
L. F. LACOUR ◽  
B. WELLS
Keyword(s):  
Fine Structure ◽  
Light Microscope ◽  
Mitotic Cycle ◽  
The Other ◽  
Masking Effect ◽  
Root Tips ◽  
Metaphase Chromosomes ◽  
Differential Reactivity ◽  
Scilla Sibirica ◽  
Electron Microscopes

With the use of the light and electron microscopes, the chromosomes of Fritillaria lanceolata and Scilla sibirica are shown to differ in respect of the heterochromatin they contain. In root meristems of the former, the heterochromatic regions (H-segments) were recognizable at all phases of the mitotic cycle by their slighter opacity to electrons than that of euchromatic parts. This was due both to less tight packing of the chromatin fibrils and lower opacity of the fibrils themselves, even though both had the same diameter, about 3 nm. In root tips of the Scilla, the heterochromatin was invariably of similar opacity to euchromatin and thus only recognizable at telophase and interphase as large chromocentres. The opacity to electrons in the heterochromatin of the 2 species, was at all times closely paralleled by the staining behaviour seen with the light microscope in sections (0.07-0.5 µm in thickness) stained with toluidine blue. The disparity in the Fritillaria, as seen in sections with the light microscope, in respect of the stainability of the hetero- and euchromatin, was masked in Feulgen squash preparations of root tips from plants grown at 18-20 °C; at metaphase by the thickness of the chromosomes and at interphase by the density of the chromocentres. When, on the other hand, the plants were grown for 4 days at 2 °C, the masking effect of thickness was circumvented in metaphase chromosomes by differential super-contraction in euchromatin. The implications of these findings in respect to previous interpretations of the differential reactivity of H-segments to low temperature, as well as the phenomenon of enhanced and reduced fluorescence in these segments with fluorochromes are discussed.

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