Quantitave, Qualitative and Antifungal Activities of Essential Oils Extract from Two Eucalyptus Species (E. sideroxylon and E. gomphocephala).

Essential oils, also known as volatile oils, are substances produced through the secondary metabolism of plants. In this study, we determined the chemical composition and the in vitro and in vivo antifungal activity of the essential oils from four species of Eucalyptus, Eucalyptus citriodora, Eucalyptus camaldulensis, Eucalyptus grandis and Eucalyptus microcorys, against the Hemileia vastatrix fungus. The essential oils from these four species of Eucalyptus were extracted from their leaves by the hydrodistillation technique using a modified Clevenger apparatus. The chemical characterization was performed by gas chromatography coupled with a mass spectrometer detector and by gas chromatography using a flame ionization detector. The antifungal activities of the essential oils against H. vastatrix were studied by evaluating the percentage of spore germination using the microdilution test for in vitro assays. The curative and preventive effects were evaluated in in vivo tests. The principal constituents of the essential oil from E. citriodora were citronellal, citronellol and isopulegol, while E. camaldulensis produced 1,8-cineole, α-terpineol and α-pinene. 1,8-cineole, α-pinene and α-terpineol were obtained from E. grandis and 1,8-cineole, α-pinene and trans-pinocarveol were the principal components in the essential oil of E. microcorys. In vitro and in vivo antifungal activities against the fungus under study were observed for most of the essential oils, except the essential oil from E. microcorys, for which no preventive antifungal activity was observed. Only the curing of infection by the H. vastatrix fungus was observed with this oil.

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Chemical Composition and Antifungal Activities of Essential Oils ofSatureja thymbraL. andSalvia pomiferassp.calycina(Sm.) Hayek

Journal of Essential Oil Research ◽

10.1080/10412905.2006.9699404 ◽

2006 ◽

Vol 18 (1) ◽

pp. 115-117 ◽

Cited By ~ 21

Author(s):

Jasmina Glamočlija ◽

Marina Soković ◽

Jelena Vukojević ◽

Ivanka Milenković ◽

L. J.L.D. Van Griensven

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Antifungal Activities

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Biogenic Emission and Essential Oils of Some Eucalyptus Species: A Comparison Study

Global Warming - Green Energy and Technology ◽

10.1007/978-1-4419-1017-2_22 ◽

2009 ◽

pp. 357-365 ◽

Cited By ~ 1

Author(s):

Yazid Foudil-Cherif ◽

Noureddine Yassaa ◽

Brahim Y. Meklati

Keyword(s):

Essential Oils ◽

Eucalyptus Species ◽

Comparison Study ◽

Biogenic Emission

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Antimicrobial Activity of Several Cineole-Rich Western Australian Eucalyptus Essential Oils

Microorganisms ◽

10.3390/microorganisms6040122 ◽

2018 ◽

Vol 6 (4) ◽

pp. 122 ◽

Cited By ~ 10

Author(s):

Fahad Aldoghaim ◽

Gavin Flematti ◽

Katherine Hammer

Keyword(s):

Mass Spectrometry ◽

Antimicrobial Activity ◽

Essential Oils ◽

Eucalyptus Globulus ◽

Antimicrobial Agents ◽

Gas Chromatography Mass Spectrometry ◽

Eucalyptus Species ◽

Broth Microdilution ◽

Main Component ◽

Western Australian

Essential oils from the Western Australian (WA) Eucalyptus mallee species Eucalyptus loxophleba, Eucalyptus polybractea, and Eucalyptus kochii subsp. plenissima and subsp. borealis were hydrodistilled from the leaves and then analysed by gas chromatography–mass spectrometry in addition to a commercial Eucalyptus globulus oil and 1,8-cineole. The main component of all oils was 1,8-cineole at 97.32% for E. kochii subsp. borealis, 96.55% for E. kochii subsp. plenissima, 82.95% for E. polybractea, 78.78% for E. loxophleba 2, 77.02% for E. globulus, and 66.93% for E. loxophleba 1. The Eucalyptus oils exhibited variable antimicrobial activity determined by broth microdilution, with E. globulus and E. polybractea oils showing the highest activities. The majority of microorganisms were inhibited or killed at concentrations ranging from 0.25% to 8.0% (v/v). Enterococcus faecalis and Candida albicans were the least susceptible organisms, whilst Acinetobacter baumannii was the most sensitive. In conclusion, all oils from WA Eucalyptus species showed microorganism inhibitory activity, although this varied according to both the Eucalyptus species and the microorganism tested. These data demonstrate that WA Eucalyptus oils show activity against a range of medically important pathogens and therefore have potential as antimicrobial agents.

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Evaluation of vapor-phase antifungal activities of selected plant essential oils against fungal strains growing on bread food model

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1483 ◽

2021 ◽

Vol 15 ◽

pp. 210-217

Author(s):

Veronika Valková ◽

Hana Ďúranová ◽

Lucia Galovičová ◽

Eva Ivanišová ◽

Miroslava Kačániová

Keyword(s):

Growth Inhibition ◽

Essential Oils ◽

Vapor Phase ◽

Mycelial Growth ◽

Antioxidant Activities ◽

Colony Growth ◽

Promising Alternative ◽

Antifungal Activities ◽

In Situ Conditions ◽

Dpph Method

The current study aimed to investigate antifungal activities of two commercially available essential oils (EOs), specifically Tea tree oil (Melaleuca alternifolia; TTEO) and St. John's wort oil (Hypericum perforatum; HPEO) against three Penicillium (P.) species: P. citrinum, P. expansum, and P. crustosum in in situ conditions. For this purpose, EOs were applied in the vapor phase to determine the growth inhibition of fungi artificially inoculated on sliced bread. Changes in colony growth rate were evaluated as markers for the mycelial growth inhibition (MGI) effect of the EOs. The antioxidant activities of the EOs were evaluated using the DPPH method. The moisture content (MC) and water activity (aw) of bread as a substrate for fungal growth were also measured. From the DPPH assay, we have found that both EOs (TTEO, HPEO) exhibited strong antioxidant activity (64.94 ±7.34%; 70.36 ±1.57%, respectively). The values for bread MC and aw were 43.01 ±0.341% and 0.947 ±0.006, respectively. Our results suggest that HPEO is the only weak inhibitor of P. citrinum and P. crustosum colony growths. Also, the highest concentrations of TTEO display only the weak capability of mycelial growth inhibition of P. citrinum and P. crustosum. By contrast, the colony growth of P. expansum was enhanced by both EOs at all levels used. In conclusion, the application of both EOs in the vapor phase against selected Penicillium species seems not to be a promising alternative to chemical inhibitors used for bread preservation.

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