scholarly journals Evaluation of vapor-phase antifungal activities of selected plant essential oils against fungal strains growing on bread food model

10.5219/1483 ◽  
2021 ◽  
Vol 15 ◽  
pp. 210-217
Author(s):  
Veronika Valková ◽  
Hana Ďúranová ◽  
Lucia Galovičová ◽  
Eva Ivanišová ◽  
Miroslava Kačániová
Keyword(s):  
Growth Inhibition ◽  
Essential Oils ◽  
Vapor Phase ◽  
Mycelial Growth ◽  
Antioxidant Activities ◽  
Colony Growth ◽  
Promising Alternative ◽  
Antifungal Activities ◽  
In Situ Conditions ◽  
Dpph Method

The current study aimed to investigate antifungal activities of two commercially available essential oils (EOs), specifically Tea tree oil (Melaleuca alternifolia; TTEO) and St. John's wort oil (Hypericum perforatum; HPEO) against three Penicillium (P.) species: P. citrinum, P. expansum, and P. crustosum in in situ conditions. For this purpose, EOs were applied in the vapor phase to determine the growth inhibition of fungi artificially inoculated on sliced bread. Changes in colony growth rate were evaluated as markers for the mycelial growth inhibition (MGI) effect of the EOs. The antioxidant activities of the EOs were evaluated using the DPPH method. The moisture content (MC) and water activity (aw) of bread as a substrate for fungal growth were also measured. From the DPPH assay, we have found that both EOs (TTEO, HPEO) exhibited strong antioxidant activity (64.94 ±7.34%; 70.36 ±1.57%, respectively). The values for bread MC and aw were 43.01 ±0.341% and 0.947 ±0.006, respectively. Our results suggest that HPEO is the only weak inhibitor of P. citrinum and P. crustosum colony growths. Also, the highest concentrations of TTEO display only the weak capability of mycelial growth inhibition of P. citrinum and P. crustosum. By contrast, the colony growth of P. expansum was enhanced by both EOs at all levels used. In conclusion, the application of both EOs in the vapor phase against selected Penicillium species seems not to be a promising alternative to chemical inhibitors used for bread preservation.

scholarly journals Seasonal Study of Methyleugenol Chemotype of Ocimum campechianum Essential Oil and Its Fungicidal and Antioxidant Activities

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Pablo Luis B. Figueiredo ◽  
Sebastião G. Silva ◽  
Lidiane D. Nascimento ◽  
Alessandra R. Ramos ◽  
William N. Setzer ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Mycelial Growth ◽  
Fungicidal Activity ◽  
Antioxidant Activities ◽  
Biological Properties ◽  
Dpph Radical ◽  
Ocimum Species ◽  
Main Component ◽  
High Antifungal Activity

Ocimum species (Lamiaceae) have aroused great interest to the scientific community with respect to different biological properties attributed to their essential oils. The seasonal variation and antioxidant and fungical activities were carried out for the essential oil of Ocimum campechianum. Its essential oil showed an excellent yield (0.5–5.3%) throughout the season. The essential oils of leaves/stems and inflorescences were analyzed by GC and GC-MS to identify their volatile constituents and associate them with the antioxidant and antifungal activities. Methyleugenol was the main component in the leaves/stems (80.0-87.0%) and inflorescences (75.3–83.5%). The essential oil and the methyleugenol standard showed low antioxidant activity (<40%) against DPPH radical, but high antifungal activity against Fusarium oxysporum and Colletotrichum gossypii phytopatogens as well as the mycelial growth and spore germination of the fungi. The high levels of methyleugenol coupled with good fungicidal activity give great agroindustrial potential to this Ocimum species.

scholarly journals EVALUATION OF DIFFERENT ESSENTIAL OILS AND BIO CONTROL AGENTS AGAINST ALTERNARIA ALTERNATA THE CAUSAL AGENT OF FRUIT ROT OF JUJUBE

2020 ◽  
Vol 1 (1) ◽  
pp. 1-8
Author(s):  
Keyword(s):  
Growth Inhibition ◽  
Essential Oils ◽  
Alternaria Alternata ◽  
Colony Growth ◽  
Causal Agent ◽  
Biocontrol Agents ◽  
Fruit Rot ◽  
Pathogenicity Test ◽  
Neem Oil

This study was carried out on the evaluation of different essential oils and biocontrol agents against Alternaria alternata the causal agent of fruit rot. For the pathogenicity test against A. alternata was performed through cut and injection inoculation methods. The antifungal potential of different essential oils like turpentine, laung, neem and castus root was carried out at different doses i.e. 5, 10 and 15% by food poisoned method to find out the effective and suitable oil for the growth inhibition of A. alternata and evaluate the effect of some biocontrol agents on growth inhibition of A. alternata. The findings of this investigation stated that cut method of inoculation showed higher percentage (2.60) of rotting as compared to injection method of inoculation (2.35). Minimum colony growth of A. alternata (31.60, 21.25 and 15.16%) was examined under Laung oil at the dosage of 5, 10 and 15% followed by Neem oil (42.60, 31.60 and 21.30%), respectively. Maximum colony growth of A. alternata (62.71, 52.40 and 41.75%) was observed under Castus root oil at the dosage of 5, 10 and 15%. Zero growth of target pathogen was examined under Turpentine at 5, 10 and 15%. Under control the A. alternata showed (90 mm) colony growth. Minimum linear colony growth of A. alternata was observed for Hypoxylon Sp1 (50.31%), followed by Neurospora spp. (52.97%), Lasiodiplodia theobromae (54.7%), Chactomium subaffine (57.07%) and Fusarium sp. (65.4%). Maximum mycelial colony growth (90%) was recorded in control. Based on present investigation, Similarly, for controlling the linear colony growth of A. alternata under in vitro conditions Turpentine oil ranked 1st, Laung oil ranked 2nd, Neem oil ranked 3rd, Castus root oil ranked 4th.

scholarly journals MOLECULAR IDENTIFICATION OF FUNGI ISOLATED FROM POSTHARVEST MANGO FRUITS AND EVALUATION OF ESSENTIAL OILS ACTIVITY OF GROWTH INHIBITION

2021 ◽  
Vol 9 (3) ◽  
pp. 178-189
Author(s):  
Adilson Ricken Schuelter ◽  
Fernando Otaviano Da Silva ◽  
Rosane Dos Santos Grignet ◽  
Debora Pereira ◽  
Rafael Francisco Da Silva ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Essential Oils ◽  
Molecular Identification ◽  
Mycelial Growth ◽  
Culture Medium ◽  
Microbial Control ◽  
Its Region ◽  
Neofusicoccum Parvum ◽  
Identification Of Fungi ◽  
Chemical Products

This work aimed to perform the molecular identification of fungi mango fruits, in addition to verifying the effect of the essential oils for microbial control. Pericarp segments containing injured and healthy areas of mango fruits were initially disinfested, and then incubated in PDA culture medium at 25 °C. From all isolated material, UDC03, UDC04, UDC09 and UDC12 were preselected, which presented homogeneous cultures. The UDC04 and UDC12 isolates were submitted to the sequencing of the ITS region for presenting mycelial growth both greater and morphologically distinct of fungi from Colletotrichum genus. When BLAST is used in species identification, it was observed that UDC04 and UDC12 belong to Botryosphaeria dothidea and Neofusicoccum parvum, respectively. Regarding the control of UDC04, it was observed that BDA culture medium containing 1.5 to 2% of lemongrass and eucalyptus oils resulted in complete inhibition. For UDC12, mycelial growth inhibition was detected with the use of 1% and 1.5% essential oils of eucalyptus and lemongrass, respectively. The chia essential oil was not effective for the isolates control. Finally, the results reveal the importance of molecular identification of these phytopathogens associated with the establishment of an alternative control of fruits due to problems of contamination with chemical products.

scholarly journals Chemical Compositions and Antioxidant Activities of Indonesian Citrus Essential Oils and Their Elucidation Using Principal Component Analysis

2020 ◽  
Author(s):  
Euis Julaeha ◽  
Kristin Shinta Dewi ◽  
Mohamad Nurzaman ◽  
Tatang Wahyudi ◽  
Tati Herlina ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Principal Component ◽  
Component Analysis ◽  
Chemical Compositions ◽  
Dpph Method ◽  
Ic50 Values ◽  
Citrus Essential Oils

Citrus essential oils (EOs) have various bioactivities like antioxidants, with many applications. Antioxidant activities depend on the chemical compositions of the EOs, which are affected by climate, soil, and geographical region. Thus, investigations on chemical compositions and antioxidant activities of Citrus EOs in different countries are valuable. In this study, we distilled EOs from peels of Indonesian-grown Citrus, including C. nobilis, C. limon, C. aurantifolia, C. amblycarpa, and Citrus spp.Chemical compositions of EOs were analyzed using Gas Chromatography-Mass Spectrometer (GC-MS), whereas the antioxidant activities were determined by employing 2,2-diphenyl-2-picrylhydrazyl (DPPH) method. Furthermore, principal component analysis (PCA) was applied to elucidate the main contributing compounds for antioxidant activity. The results show that all EOs possess unique chemical characteristics, with limonene as the majority constituent. For antioxidant activities, C. limon and C. amblycarpa EOs are the two strongest, IC50 values below 7.00 &mu;L/mL. PCA approach suggests that -terpinene mainly contributes to the high antioxidant activities of C. limon and C. amblycarpa. Moreover, o-cymene, thymol, p-cymene, and &alpha;-pharnesene may also be responsible for the antioxidant activity of C. limon EO. These results are valuable information for the applications of Citrus EOs as antioxidant sources.

scholarly journals Liquid and Vapor Phase of Four Conifer-Derived Essential Oils: Comparison of Chemical Compositions and Antimicrobial and Antioxidant Properties

2021 ◽  
Vol 14 (2) ◽  
pp. 134 ◽  
Author(s):  
Stefania Garzoli ◽  
Valentina Laghezza Masci ◽  
Valentina Caradonna ◽  
Antonio Tiezzi ◽  
Pierluigi Giacomello ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Essential Oils ◽  
Vapor Phase ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Abies Alba ◽  
Gas Chromatography Mass Spectrometry ◽  
Minimal Bactericidal Concentration ◽  
Chemical Compositions ◽  
Bacterial Strains

In this study, the chemical composition of the vapor and liquid phase of Pinus cembra L., Pinus mugo Turra, Picea abies L., and Abies Alba M. needles essential oils (EOs) was investigated by Headspace-Gas Chromatography/Mass Spectrometry (HS-GC/MS). In the examined EOs, a total of twenty-eight components were identified, most of which belong to the monoterpenes family. α-Pinene (16.6–44.0%), β-pinene (7.5–44.7%), limonene (9.5–32.5%), and γ-terpinene (0.3–19.7%) were the most abundant components of the liquid phase. Such major compounds were also detected in the vapor phase of all EOs, and α-pinene reached higher relative percentages than in the liquid phase. Then, both the liquid and vapor phases were evaluated in terms of antibacterial activity against three Gram-negative bacteria (Escherichia coli, Pseudomonas fluorescens, and Acinetobacter bohemicus) and two Gram-positive bacteria (Kocuria marina and Bacillus cereus) using a microwell dilution assay, disc diffusion assay, and vapor phase test. The lowest Minimum Inhibitory Concentration (MIC) (13.28 mg/mL) and Minimal Bactericidal Concentration (MBC) (26.56 mg/mL) values, which correspond to the highest antibacterial activities, were reported for P. abies EO against A. bohemicus and for A. alba EO against A. bohemicus and B. cereus. The vapor phase of all the tested EOs was more active than liquid phase, showing the inhibition halos from 41.00 ± 10.15 mm to 80.00 ± 0.00 mm for three bacterial strains (A. bohemicus, K. marina, and B. cereus). Furthermore, antioxidant activities were also investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis (3- ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays, and a concentration-dependent antioxidant capacity for all EOs was found. P. mugo EO showed the best antioxidant activity than the other Pinaceae EOs. The four Pinaceae EOs could be further investigated for their promising antibacterial and antioxidant properties, and, in particular, α-pinene seems to have interesting possibilities for use as a novel natural antibacterial agent.

scholarly journals EFFECTS OF ANTI-INFLAMMATORY AND ANTIOXIDANT ACTIVITIES OF LANTANA (Lantana Camara L)

2021 ◽  
Vol 6 (8) ◽  
pp. 144-151
Author(s):  
Restia Apriliana ◽  
Yufri Aldi ◽  
Sri Oktavia
Keyword(s):  
Essential Oils ◽  
Traditional Medicine ◽  
Empirical Evidence ◽  
Antioxidant Activities ◽  
Lantana Camara ◽  
Plant Leaves ◽  
Standard Drug ◽  
Dpph Method ◽  
Ic50 Values ◽  
Anti Inflammatory

Lantana camara is a plant that is used empirically for traditional medicine. This plant has the potential to be developed as an anti-inflammatory and antioxidant drug because it contains flavonoids, saponins, tannins, alkaloids[1] essential oils[19], carbohydrates, proteins, glycosides, oligosaccharides, kinins, steroids, triterpenoids, and sesquiterpenes[2]. Therefore this article is created in order to provide information about the various research that supports evidence of conformity with the use of this plant empirically as anti-inflammatory and antioxidant. Antioxidant activities were obtained in Lantana camara plants by using the DPPH method which showed high IC50 values compared to the standard drug. The anti-inflammatory activity of lantana leaves showed that they had different anti-inflammatory effects depending on the dose given. From the results of the review, empirical evidence of pharmacological activity was found from the use of the lantana plant leaves.

scholarly journals Essential Oils as Natural Antimicrobial and Antioxidant Products in the Agrifood Indus

2020 ◽  
pp. 55-69 ◽  
Author(s):  
M. Dolores Ibáñez ◽  
M. Pilar López-Gresa ◽  
Purificación Lisón ◽  
Ismael Rodrigo ◽  
José María Bellés ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pseudomonas Syringae ◽  
Antioxidant Activities ◽  
Phytopathogenic Fungus ◽  
Oregano Essential Oil ◽  
Dpph Method ◽  
Main Component ◽  
Disk Method

Consumers are aware of the dangers arising from the use of synthetic antioxidants and antimicrobials in the agrifood industry, demanding safer and “greener” alternatives. In this study, the antioxidant activity of commercial essential oils through DPPH method, their antimicrobial effects against the bacterium Pseudomonas syringae and the phytopathogenic fungus Fusarium oxysporum by means of the standardized disk method were determined. Clove along with winter savory, cinnamon and oregano essential oils as well as carvacrol showed the highest antioxidant activity comparable to reference standards. Wintergreen essential oil was the most potent inhibitor against P. syringae growth at the highest doses (20 and 10 μL). Oregano essential oil and its main component carvacrol were able to stop the bacterium growth even at the lowest treatment (1 μL). Cinnamon, oregano and peppermint essential oils inhibited F. oxysporum development at all doses (20, 10 and 5 μL) assayed. In general, most of the essential oils displayed more antifungal than antibacterial and antioxidant activities.

scholarly journals Chemical composition, antioxidant and antifungal activities of essential oils and extracts from Plectranthus spp. against dermatophytes fungi

2018 ◽  
Vol 19 (1) ◽  
pp. 105-115 ◽  
Author(s):  
Fransérgio Américo Ribeiro Alves ◽  
Selene Maia de Morais ◽  
Antonio Carlos Nogueira Sobrinho ◽  
Isaac Neto Goes da Silva ◽  
Clécio Galvão Martins ◽  
...  
Keyword(s):  
Essential Oils ◽  
Antioxidant Activities ◽  
Fungal Infections ◽  
Fungal Growth ◽  
Antifungal Drugs ◽  
Gas Chromatography Mass Spectrometry ◽  
Microsporum Canis ◽  
Synergistic Activity ◽  
Antifungal Activities ◽  
Broth Microdilution Method

SUMMARY Resistance to use antifungal drugs is a great concern seeking for scientists to discover new products to treat fungal infections. The aim of this study was to evaluate the antioxidant and antifungal activities of essential oils and extracts of Plectranthus grandis and Plectranthus ornatus against Trichophyton rubrum and Microsporum canis dermatophytes strains. Extracts were obtained from leaves by maceration in ethanol (96%) during 7 days. The oils were obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry. A total of 25 components were identified, as major constituents the sesquiterpenes β-caryophyllene, α-copaene, germacrene, β-caryophyllene and caryophyllene oxide. Antioxidant activities were evaluated using DPPH scavenging assay and antifungal action was determined by the broth microdilution method. The decocts obtained from the extraction of essential oil presented a greater antioxidant action when compared with the essential oils, with IC50 values of 12.35 μg/mL and 15.69 μg/mL to P. ornatus and P. grandis, respectively. Natural products presented significant antifungal activity, with MIC values ranging from 0.078 mg/mL to 0.31 mg/mL for all strains. The synergistic activity between Plectranthus spp. extracts and ketoconazole demonstrated a fungal growth inhibitory action when combined with a standard antifungal drug, indicating its potential for use in preventive veterinary medicine to treat dermatophytoses.

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