THE EFFECT OF ORALLY INGESTED ATORVASTATIN ON THE MASSETER MUSCLE OF WHITE ALBINO RATS (HISTOLOGICAL AND IMMUNOHISTOCHEMICAL STUDIES)

2017 ◽  
Vol 63 (1) ◽  
pp. 605-614
Author(s):  
Mohammed Shredah ◽  
Saher Sayed
Keyword(s):  
Masseter Muscle ◽  
Albino Rats ◽  
Immunohistochemical Studies

scholarly journals Distribution of Cytokeratin 17 in the Parenchymal Elements of Rat’s Submandibular Glands Subjected to Fractionated Radiotherapy

2020 ◽  
Vol 14 (03) ◽  
pp. 440-447
Author(s):  
Sherif S. Hassan ◽  
Mahmoud A. Attia ◽  
Alaa M. Attia ◽  
Reda A. Nofal ◽  
Adel Fathi
Keyword(s):  
Submandibular Gland ◽  
Apical Part ◽  
Albino Rats ◽  
Pathological Effect ◽  
Fractionated Radiotherapy ◽  
Cytokeratin 17 ◽  
Neck Malignancy ◽  
Submandibular Salivary Glands ◽  
Mitotic Figures ◽  
Immunohistochemical Studies

Abstract Objectives The aim of this research was to study the intensity of cytokeratin 17 (CK17) in the parenchymal elements of rat’s submandibular salivary glands subjected to fractionated radiotherapy regimen that used for treatment of head and neck malignancy. Materials and Methods Twenty male albino rats were divided into two equal groups (normal and irradiated). The irradiated group received a radiation dose of 5 Grays daily for 5 days using therapeutic X-ray beam. Six months later, submandibular gland was dissected out and prepared for both histological and immunohistochemical studies. Results Submandibular gland of irradiated group showed two different types of histological alterations. The first alteration showed severe gland atrophy replaced by either fibrous or fatty tissues. In some sections, the gland exhibited proliferating activity in the form of profuse amounts of mitotic figures. Immunohistochemical examination of control glands displayed a mild cytoplasmic expression of CK17 of duct cells as well as serous acini. The staining pattern was either diffused or concentrated at the basal part of the cell with negative expression at its apical part. Statistical Analysis Expression of CK17 in submandibular gland of irradiated group displayed a highly significant differences (P < 0.001) in both intercalated and striated ducts. Many serous acini displayed a highly significant differences (P < 0.001) whereas, mucous acini were negatively stained. Conclusions The intensity and diffusion of CK17 expression in our results foretell the pathological effect of radiotherapy on the intermediate filaments of salivary gland parenchyma that interfered with production and/or secretion of saliva leading to xerostomia.

PHYTOREMEDIAL POTENTIAL OF HYDROALCOHOL EXTRACT OF SEEDS OF SORGHUM BICOLOR AGAINST DRUG-INDUCED NEPHROTOXICITY

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (07) ◽  
pp. 46-52
Author(s):  
Sreedevi Adikay ◽  
Sai Sruthi Kaveripakam ◽  
Keyword(s):  
Sorghum Bicolor ◽  
Albino Rats ◽  
Prophylactic Regimen ◽  
Drug Induced ◽  
Hydroalcoholic Extract ◽  
Novel Approach ◽  
Nephroprotective Activity ◽  
Wistar Albino Rats ◽  
Immunohistochemical Studies ◽  
The Impact

The gravity of the impact of drug induced nephrotoxicity is more prominent in society. The present study was undertaken to evaluate the potential of hydroalcoholic extract of seeds of Sorghum bicolor against cisplatin and doxorubicin- induced nephrotoxicity in Wistar albino rats. The nephrotoxicity was modeled by intraperitoneal injection of cisplatin (5 mg/kg b.w.) in cisplatin model and doxorubicin (15 mg/kg b.w.) in doxorubicin-induced model in rats. Nephroprotection of hydroalcoholic extract of seeds of S. bicolor was evaluated at two different doses of 200 and 400mg/kg b.w. The nephroprotective activity was assessed by the determination of various serum and urinary parameters, anti-oxidant studies, histological and immunohistochemical studies. The results indicated that injection of cisplatin and doxorubicin led to marked nephrotoxicity in animals. Treatment with extract in cisplatin-induced model resulted in significant nephroprotective activity in a curative regimen whereas in prophylactic regimen the extract prevented the induction of nephrotoxicity only up to a considerable level. But the extract failed to attenuate the doxorubicin induced nephrotoxicity, as evident by biochemical, histological and immunohistochemical studies. From the findings, it is concluded that the seeds of S. bicolor can be used as a novel approach in the treatment of cisplatin-induced nephrotoxicity.

scholarly journals Histological and Immunohistochemical Studies on the Role of Stem Cells on the Burned Skin of Adult Male Albino Rats

2020 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Fatma Abd Allah ◽  
Ashraf Moustafa ◽  
Lotfy Moahmmed ◽  
Ezz El-Dein Abd Allah
Keyword(s):  
Stem Cells ◽  
Adult Male ◽  
Albino Rats ◽  
Burned Skin ◽  
Immunohistochemical Studies

Effects of renovascular hypertension on rat adrenal zona glomerulosa

1978 ◽  
Vol 36 (2) ◽  
pp. 582-583
Author(s):  
G. Mazzocchi ◽  
P. Rebuffat ◽  
C. Robba ◽  
P. Vassanelli ◽  
G. G. Nussdorfer
Keyword(s):  
Renovascular Hypertension ◽  
Left Kidney ◽  
Renin Angiotensin System ◽  
Plasma Renin ◽  
Zona Glomerulosa ◽  
Ultrastructural Changes ◽  
Albino Rats ◽  
Left Renal Artery ◽  
Angiotensin System ◽  
And Control

It is well known that the rat adrenal zona glomerulosa steroidogenic activity is controlled by the renin-angiotensin system. The ultrastructural changes in the rat zona glomerulosa cells induced by renovascular hypertension were described previously, but as far as we are aware no correlated biochemical and morphometric investigations were performed.Twenty adult male albino rats were divided into 2 experimental groups. One group was subjected to restriction of blood flow to the left kidney by the application of a silver clip about the left renal artery. The other group was sham-operated and served as a control. Renovascular hypertension developed in about 10 days: sistolic blood pressure averaged 165 ± 6. 4 mmHg, whereas it was about 110 ± 3. 8 mmHg in the control animals. The hypertensive and control rats were sacrificed 20 days after the operation. The blood was collected and plasma renin activity was determined by radioimmunological methods. The aldosterone concentration was radioimmunologically assayed both in the plasma and in the homogenate of the left capsular adrenal gland.

Further Studies on the Ultrastructure of Harderian Gland in Adult Rats

1974 ◽  
Vol 32 ◽  
pp. 288-289
Author(s):  
Alfredo Feria-Velasco ◽  
Guadalupe Tapia-Arizmendi
Keyword(s):  
Fine Structure ◽  
Domestic Fowl ◽  
Animal Species ◽  
Acinar Cells ◽  
Harderian Gland ◽  
Myoepithelial Cells ◽  
The Other ◽  
Albino Rats ◽  
Adult Rats ◽  
Cell Components

The fine structure of the Harderian gland has been described in some animal species (hamster, rabbit, mouse, domestic fowl and albino rats). There are only two reports in the literature dealing on the ultrastructure of rat Harderian gland in adult animals. In one of them the author describes the myoepithelial cells in methacrylate-embbeded tissue, and the other deals with the maturation of the acinar cells and the formation of the secretory droplets. The aim of the present work is to analize the relationships among the acinar cell components and to describe the two types of cells located at the perifery of the acini.

Cell Fine Structure as Revealed in Dessicator-Dried Resinless Sections

1981 ◽  
Vol 39 ◽  
pp. 622-623
Author(s):  
R. P. Becker ◽  
J. J. Wolosewick ◽  
J. Ross-Stanton
Keyword(s):  
Fine Structure ◽  
Tannic Acid ◽  
Three Dimensional ◽  
Albino Rats ◽  
Cell Organelles ◽  
Vascular Perfusion ◽  
Thin Sections ◽  
Carbon Coated ◽  
Embedding Medium ◽  
Polyethylene Glycol 6000

Methodology has been introduced recently which allows transmission and scanning electron microscopy of cell fine structure in semi-thin sections unencumbered by an embedding medium. Images obtained from these “resinless” sections show a three-dimensional lattice of microtrabeculfee contiguous with cytoskeletal structures and membrane-bounded cell organelles. Visualization of these structures, especially of the matiiDra-nous components, can be facilitated by employing tannic acid in the fixation step and dessicator drying, as reported here.Albino rats were fixed by vascular perfusion with 2% glutaraldehyde or 1.5% depolymerized paraformaldehyde plus 2.5% glutaraldehyde in 0.1M sodium cacodylate (pH 7.4). Tissues were removed and minced in the fixative and stored overnight in fixative containing 4% tannic acid. The tissues were rinsed in buffer (0.2M cacodylate), exposed to 1% buffered osmium tetroxide, dehydrated in ethyl alcohol, and embedded in pure polyethylene glycol-6000 (PEG). Sections were cut on glass knives with a Sorvall MT-1 microtome and mounted onto poly-L-lysine, formvar-carbon coated grids while submerged in a solution of 95% ethanol containing 5% PEG.

Sign in / Sign up

Export Citation Format

Share Document