scholarly journals ASSESSMENT OF GENETIC DIVERSITY OF TOMATO (Lycopersicon esculentum L.) GERMPLASM USING MOLECULAR MARKERS (RAPD AND ISSR)

2013 ◽  
Vol 42 (1) ◽  
pp. 163-182
Author(s):  
NEVEEN HASSAN ◽  
SHIMAA MOSTAFA ◽  
A. TWFIK
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Lycopersicon Esculentum ◽  
Rapd And Issr

Genetic diversity analysis of advanced breeding lines derived from interspecific and intervarietal crosses of black gram based on morphological and molecular markers

2018 ◽  
Author(s):  
R. Devi ◽  
R. K. Mittal ◽  
V. K. Sood ◽  
P. N. Sharma
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Interspecific Cross ◽  
Resistance Breeding ◽  
Breeding Lines ◽  
Correlation Studies ◽  
Yield Determination ◽  
Derivatives Of ◽  
Rapd And Issr ◽  
Number Of Seeds

The main objective of this study was to assess the genetic diversity of 34 genotypes comprising of 25 advanced derivatives of interspecific cross between V. mungo x V. umbellata, five intervarietal crosses along with four checks (including blackgram parents) using 11 morphological traits and molecular markers (RAPD and ISSR) and for their resistance towards Cercospora, anthracnose and MYMV. The lines Palampur-93 x BRS-1 (236-A-L-1-4), HPBU-35 and HPBU-111 were found statistically at par to the best check Him Mash-1 for seed yield. Correlation studies revealed that the selection should be based on large seeds, more number of pods per plant and more number of seeds per pod indicating the importance of these traits in yield determination. The cluster analysis on molecular basis grouped the blackgram parents and advance lines differently than at morphological level which revealed genetic variation among genotypes and also confirmed that rigorous selection had been made for blackgram types in segregating generations rather than ricebean types as most of the advance derivatives of V. mungo x V. umbellata were found to be concentrated near blackgram parents. The lines PDU-1 x PRR-1 (62-3-L-10-1) and Palampur-93 x PRR-1 (258-1L-2-5) were found resistant to both Cercospora and anthracnose while, Palampur-93 x BRS-1 (236-A-L-3-2) for MYMV. The information generated from this study would be helpful in characterizing the advanced derived lines and parents in the selection and utilization of diverse genotypes to enhance variability and productivity along with resistance breeding of V. mungo.

Using RAPD and ISSR Molecular Markers to Analyze the Genetic Diversity of Rose scented Pelargonium Populations

2017 ◽  
Vol 33 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Qing-yuan Yi ◽  
Shao-wen Yi ◽  
Tai-feng Qin ◽  
Cheng-yun Li
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Issr Molecular Markers ◽  
Rapd And Issr

scholarly journals Evaluation of Genetic Diversity among Some Common Fig Using RAPD and ISSR Molecular Markers

2020 ◽  
Vol 6 (2) ◽  
pp. 43-54
Author(s):  
Batol Gheitarani ◽  
Javad Erfani-Moghadam ◽  
Arash Fazeli ◽  
◽  
◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Common Fig ◽  
Issr Molecular Markers ◽  
Rapd And Issr

scholarly journals Genetic Relatedness among Cultivars of the Greek Plum Germplasm

2013 ◽  
Vol 41 (2) ◽  
pp. 491 ◽  
Author(s):  
Ioannis ATHANASIADIS ◽  
Nikolaos NIKOLOUDAKIS ◽  
Marianna HAGIDIMITRIOU
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Background ◽  
Genetic Relatedness ◽  
Germplasm Collection ◽  
Prunus Domestica ◽  
Discrimination Power ◽  
Breeding Programs ◽  
Issr Molecular Markers ◽  
Rapd And Issr

Genetic diversity of the Greek plum germplasm collection was investigated using a combined RAPD and ISSR molecular markers approach. Twenty-six genotypes held at NAGREF-Naoussa were analyzed, producing in total 150 loci, of which 116 were polymorphic. Both techniques were highly informative and had a discrimination power greater than 0.9. RAPD and ISSR dendrograms were fairly correlated. The accessions were clustered according to ploidy and species. All Prunus domestica genotypes were grouped together and showed greater similarity to P. insititia and P. cerasifera genotypes compared to P. salicina, which was found genetically diverged. Bayesian structural analysis revealed significant admixture among genotypes. Greek varieties P. domestica ‘Goulina’ and ‘Asvestochoriou’ exhibited a distinctive genetic background, differentiating them from foreign varieties. This feature could make them attractive for breeding programs, since they can increase genetic diversity.

Genetic Diversity of Basil (Ocimum spp. - Lamiaceae) Based on RAPD Markers and Volatile Oil Constituents

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 492f-493
Author(s):  
Roberto F. Vieira ◽  
James E. Simon ◽  
Peter Goldsbrough ◽  
Antonio Figueira
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Volatile Oil ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Traditional Medicines ◽  
The Tropics ◽  
High Degree ◽  
First Time

Essential oils extracted from basil (Ocimum spp.) by steam distillation are used to flavor foods, oral products, in fragrances, and in traditional medicines. The genus Ocimum contains around 30 species native to the tropics and subtropics, with some species naturalized and/or cultivated in temperate areas. Interand intraspecific hybridization have created significant confusion in the botanical systematics of this genus. Taxonomy of basil (O. basilicum) is also complicated by the existence of numerous varieties, cultivars, and chemotypes within the species that do not differ significantly in morphology. In this study we are using RAPD markers and volatile oil composition to characterize the genetic diversity among the most economically important Ocimum species. We hypothesize that the genetic similarity revealed by molecular markers will more accurately reflect the morphological and chemical differences in Ocimum than essential oil composition per se. Preliminary research using five Ocimum species, four undetermined species, and eight varieties of O. basilicum (a total of 19 accessions) generated 107 polymorphic fragments amplified with 19 primers. RAPDs are able to discriminate between Ocimum species, but show a high degree of similarity between O. basilicum varieties. The genetic distance between nine species and among 55 accessions within the species O. americanum, O. basilicum, O. campechianum, O. × citriodorum, O. gratissimum, O. kilimandscharium, O. minimum, O. selloi, and O. tenuiflorum will be analyzed by matrix of similarity and compared to the volatile oil profile. This research will for the first time apply molecular markers to characterize the genetic diversity of Ocimum associate with volatile oil constituent.

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