Characterization of The Decellularized Male Rabbit Kidney as A Three-Dimensional Natural Scaffold for Tissue Engineering. A Histological Study

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Sara Elsebay ◽  
Ayat Abdelnaby ◽  
Gehan Khalaf ◽  
Naglaa Abou-Rabia
Keyword(s):  
Tissue Engineering ◽  
Histological Study ◽  
Three Dimensional ◽  
Rabbit Kidney ◽  
Male Rabbit

Hydroxyapatite/Biopolymers Composite Scaffolds for Bone Tissue Engineering

2011 ◽  
Vol 493-494 ◽  
pp. 826-831
Author(s):  
A.C.B.M. Fook ◽  
Thiago Bizerra Fideles ◽  
R.C. Barbosa ◽  
G.T.F.S. Furtado ◽  
G.Y.H. Sampaio ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Freeze Drying ◽  
Three Dimensional ◽  
Porous Scaffolds ◽  
X Ray Diffraction ◽  
X Ray ◽  
Interconnected Pores

The application of a hybrid composite consisting of biopolymer and calcium phosphate, similar morphology and properties of natural bone, may be a way to solve the problem of the fragility of ceramics without reducing its mechanical properties, retaining the properties of biocompatibility and high bioactivity. This work aims at the preparation and characterization of three-dimensional scaffolds composite HA / biopolymers (chitosan and gelatin). The freeze-drying technique was employed in this study to obtain these frameworks and partial results showed the effectiveness of this method. This involved the study of structural, chemical and morphological frameworks, in order to direct the research suggested the application. The X Ray Diffraction (XRD) and infrared spectroscopy and Fourier transform (FTIR) results confirmed the formation of hydroxyapatite (HA) phase and the presence of characteristic bands of HA and biopolymers in all compositions. The microstructure of the scaffolds study conducted by Scanning Electron Microscopy (SEM) revealed the formation of longitudinally oriented microchannels with interconnected pores. In all compositions the porous scaffolds showed varying sizes and mostly larger than 100μm, and is therefore considered materials with potential for application in bone tissue engineering.

scholarly journals MicroCT analysis of connectivity in porous structures: optimizing data acquisition and analytical methods in the context of tissue engineering

2020 ◽  
Vol 17 (165) ◽  
pp. 20190833
Author(s):  
Malavika Nair ◽  
Jennifer H. Shepherd ◽  
Serena M. Best ◽  
Ruth E. Cameron
Keyword(s):  
Tissue Engineering ◽  
Analytical Methods ◽  
Large Scale ◽  
Structural Parameters ◽  
Three Dimensional ◽  
Data Capture ◽  
Pixel Size ◽  
Scale Invariant ◽  
Structural Anisotropy

Micro-computed X-ray tomography (MicroCT) is one of the most powerful techniques available for the three-dimensional characterization of complex multi-phase or porous microarchitectures. The imaging and analysis of porous networks are of particular interest in tissue engineering due to the ability to predict various large-scale cellular phenomena through the micro-scale characterization of the structure. However, optimizing the parameters for MicroCT data capture and analyses requires a careful balance of feature resolution and computational constraints while ensuring that a structurally representative section is imaged and analysed. In this work, artificial datasets were used to evaluate the validity of current analytical methods by considering the effect of noise and pixel size arising from the data capture, and intrinsic structural anisotropy and heterogeneity. A novel ‘segmented percolation method’ was developed to exclude the effect of anomalous, non-representative features within the datasets, allowing for scale-invariant structural parameters to be obtained consistently and without manual intervention for the first time. Finally, an in-depth assessment of the imaging and analytical procedures are presented by considering percolation events such as micro-particle filtration and cell sieving within the context of tissue engineering. Along with the novel guidelines established for general pixel size selection for MicroCT, we also report our determination of 3 μm as the definitive pixel size for use in analysing connectivity for tissue engineering applications.

Histological study of an experimentally constructed decellularized rat lung as a model of whole organ three-dimensional natural scaffold

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Marium Romany Abdelsayed ◽  
Suzi Sobhy Atalla ◽  
Gehan Khalaf Megahed ◽  
Asmaa Abd El-Monem Abo Zeid
Keyword(s):  
Tissue Engineering ◽  
Histological Study ◽  
Three Dimensional ◽  
Nuclear Material ◽  
Biological Properties ◽  
Basement Membranes ◽  
Elastic Fibers ◽  
Scientific Exploration ◽  
And Migration ◽  
End Stage

Abstract Introduction With the increase of end stage lung diseases and the great problems facing lung transplantation tissue engineering become a promising solution. The first step in lung engineering is to obtain a 3D Extracellular matrix lung scaffold via decellularization. Decellularization aims to remove cells from tissue ultrastructure while preserving the mechanical and biological properties of the tissue. Intact ECM provides critical cues for differentiation and migration of cells that are seeded onto the organ scaffold. Objectives This study aimed to obtain an intact and well-preserved ECM lung scaffold by decellularization of rat lungs. Methods Decellularization of lungs of ten Wistar rats was achieved by perfusing detergents through the pulmonary artery. The resultant scaffolds were fixed and analyzed histologically. Results It was found that the decellularization process effectively removed the cellular and nuclear material while retaining native the 3D ECM of lung tissue. The architecture of the collagen and elastic fibers networks were preserved as comparable to the native lungs. Furthermore, the basement membranes of the bronchiolar and interalveolar septa were intact. Conclusions This methodology is expected to allow decellularization of human lung tissues and permits future scientific exploration in tissue engineering.

Preparation and characterization of a three-dimensional printed scaffold based on a functionalized polyester for bone tissue engineering applications

2011 ◽  
Vol 7 (5) ◽  
pp. 1999-2006 ◽  
Author(s):  
Hajar Seyednejad ◽  
Debby Gawlitta ◽  
Wouter J.A. Dhert ◽  
Cornelus F. van Nostrum ◽  
Tina Vermonden ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Engineering Applications

Mechanical Characterization of Electrospun Polycaprolactone (PCL): A Potential Scaffold for Tissue Engineering

2008 ◽  
Vol 130 (1) ◽  
Author(s):  
Ryan R. Duling ◽  
Rebecca B. Dupaix ◽  
Noriko Katsube ◽  
John Lannutti
Keyword(s):  
Tissue Engineering ◽  
Mechanical Behavior ◽  
Three Dimensional ◽  
Tensile Tests ◽  
Material Behavior ◽  
Chain Model ◽  
Viable Option ◽  
Advantages And Disadvantages ◽  
Large Stretch

This paper investigates the mechanical behavior of electrospun polycaprolactone (PCL) under tensile loading. PCL in bulk form degrades slowly and is biocompatible, two properties that make it a viable option for tissue engineering applications in biomedicine. Of particular interest is the use of electrospun PCL tubes as scaffolds for tissue engineered blood vessel implants. Stress relaxation and tensile tests have been conducted with specimens at room temperature (21°C) and 37°C. Additionally, to probe the effects of moisture on mechanical behavior, specimens were tested either dry (in air) or submerged in water. In general, the electrospun PCL was found to exhibit rate dependence, as well as some dependence on the test temperature and on whether the sample was wet or dry. Two different models were investigated to describe the experimentally observed material behavior. The models used were Fung’s theory of quasilinear viscoelasticity (QLV) and the eight-chain model developed for rubber elastomers by Arruda and Boyce (1993, “A Three-Dimensional Constitutive Model for the Large Stretch Behavior of Rubber Elastic Materials,” J. Mech. Phys. Solids, 41(2), pp. 389–412). The implementation and fitting results, as well as the advantages and disadvantages of each model, are presented. In general, it was found that the QLV theory provided a better fit.

scholarly journals Three-dimensional morphometric analysis of the renal vasculature

2018 ◽  
Vol 314 (5) ◽  
pp. F715-F725 ◽  
Author(s):  
Zohaib Khan ◽  
Jennifer P. Ngo ◽  
Bianca Le ◽  
Roger G. Evans ◽  
James T. Pearson ◽  
...  
Keyword(s):  
Morphometric Analysis ◽  
Three Dimensional ◽  
Image Resolution ◽  
Rabbit Kidney ◽  
Renal Vasculature ◽  
Automated Method ◽  
Precise Characterization ◽  
Vascular Geometry ◽  
Renal Vascular

Vascular topology and morphology are critical in the regulation of blood flow and the transport of small solutes, including oxygen, carbon dioxide, nitric oxide, and hydrogen sulfide. Renal vascular morphology is particularly challenging, since many arterial walls are partially wrapped by the walls of veins. In the absence of a precise characterization of three-dimensional branching vascular geometry, accurate computational modeling of the intrarenal transport of small diffusible molecules is impossible. An enormous manual effort was required to achieve a relatively precise characterization of rat renal vascular geometry, highlighting the need for an automated method for analysis of branched vasculature morphology to allow characterization of the renal vascular geometry of other species, including humans. We present a semisupervised method for three-dimensional morphometric analysis of renal vasculature images generated by computed tomography. We derive quantitative vascular attributes important to mass transport between arteries, veins, and the renal tissue and present methods for their computation for a three-dimensional vascular geometry. To validate the algorithm, we compare automated vascular estimates with subjective manual measurements for a portion of rabbit kidney. Although increased image resolution can improve outcomes, our results demonstrate that the method can quantify the morphological characteristics of artery-vein pairs, comparing favorably with manual measurements. Similar to the rat, we show that rabbit artery-vein pairs become less intimate along the course of the renal vasculature, but the total wrapped mass transfer coefficient increases and then decreases. This new method will facilitate new quantitative physiological models describing the transport of small molecules within the kidney.

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