Fatigue Behaviour of Connectors used in Cable Harnessing through Cavity Formation Related Microstructural Degradation: A Failure Investigation Perspective

Two separately failed electrical connector pieces during a vibration test were received for failure analysis. Chemical composition, hardness values and microstructures of the each of the connector material indicate that the material of construction is a die cast aluminium-silicon type of alloy, closely matching with the standard ANSI/AA B380 alloy. Intergranular and faceted fracture features are observed and failure mechanism is found to be fatigue dominated. The connectors failed by impact fatigue arising out of the loosening of the connector assembly. This has happened by cavity formation and/or growth related microstructural degradation processes. Initial casting pores as well as microstructural degradations such as interconnected pores have developed in service and their successive growth, decohesion and interconnection of each of primary Si particles and Al-Fe-Mn precipitates (along precipitate-matrix interface) have led the initiation of the crack under fatigue loading. Brittle as-cast microstructure (as typified by the precipitate-matrix interfacial cracking), existing vibratory loading and absence of any rise in temperature in the system have assisted the initial cavity (crack) formation and/or growth. Moreover, initial fitment related looseness is an additional factor in initiating and propagating this damaging mechanism.

Conducting Cytocompatible Scaffolds: Composite of Sodium Hyaluronate and Colloidal Particles of Conducting Polymers

Novel bio-inspired conductive scaffolds composed of sodium hyaluronate containing water soluble polyaniline or polypyrrole colloidal particles (concentrations 0.108, 0.054 and 0.036 % w/w) were manufactured. For this purpose, either crosslinking with N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid or a freeze-thawing process in the presence of poly(vinylalcohol) were used. The scaffolds comprised interconnected pores with prevailing porosity values of ~30 % and pore sizes enabling the accommodation of cells. Good swelling capacity (92 – 97 %) without any sign of disintegration was typical for all samples. The elasticity modulus depended on the composition of the scaffolds, with the highest value of ~50 000 Pa obtained for the sample containing the highest content of polypyrrole particles. The scaffolds did not possess cytotoxicity and allowed cell adhesion and growth on the surface. Using the in vivo-mimicking conditions in a bioreactor, cells were also able to grow into the structure of the scaffolds. The technique of scaffold preparation used here thus overcomes the limitations of conducting polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.

Preparation and Characterization of Calcium Cross-Linked Starch Monolithic Cryogels and Their Application as Cost-Effective Green Filters

Monolithic cryogels from starch were successfully synthesized and applied as alternative biodegradable filters for the first time. Rice flour was cross-linked with Ca2+ from limewater during gelatinization before being frozen and then thawed for three cycles. The resultant material was then soaked in ethanol for 3 h before incubation at 80 °C for 1 h, yielding monolithic material with interconnected pores in sizes of 51 ± 18 to 52 ± 15 µm without any need of freeze-drying. The cryogels possessed macroporous structure with specific surface areas from 1.1 to 4.3 m2g−1, they could adsorb water from 599 ± 27 to 635 ± 59% of their dry weight with low swelling ratios of 6.0 ± 0.3 to 6.4 ± 0.6 gwater/gcryogel, and could be applied as biofilters to remove suspended particles and reduce the light absorption of water sample from 25 ± 3 to 96 ± 5%. The prepared biofilters can be re-used up to three times, although they cost only USD 0.0004/piece. Complete weight loss resulted from burial in soil for 30 days, indicating environmentally friendly biodegradation and potential for environmental applications.

Parallel or Interconnected Pores’ Formation through Etchant Selective Silicon Porosification

Effect of oxidizer, present in the etching solution, on the surface morphology and microstructure obtained after porosification of p-type silicon wafer using metal assisted chemical etching has been studied here. The morphologies of Si wafers porosified using two different solutions namely HF/ H2O2 and HF/KMnO4 have been compared to establish how either of the oxidizers (H2O2 or KMnO4) should be chosen depending on the desired application. The comparative study reveals that either parallel pores with wire like structures or interconnected pores with cheese like structures can be obtained when H2O2 or KMnO4 respectively are chosen. Careful analysis of SEM images has been carried out using ImageJ to establish that samples prepared using KMnO4 are more porous due to aggressive etching. Additionally, experimental and theoretical Raman spectroscopic studies have been utilized to study the presence of low dimensional Si nanostructures of a few nanomaters size at the microscopic level in the porosified silicon.

The Open Cell Form of 3D-Printed Titanium Improves Osteconductive Properties and Adhesion Behavior of Dental Pulp Stem Cells

Titanium specimens have been proven to be safe and effective biomaterials in terms of their osseo-integration. To improve the bioactivity and develop customized implants titanium, the surface can be modified with selective laser melting (SLM). Moreover, the design of macro-porous structures has become popular for reaching a durable bone fixation. 3D-printed titanium (Titanium A, B, and C), were cleaned using an organic acid treatment or with electrochemical polishing, and were characterized in terms of their surface morphology using scanning electron microscopy. Next, Dental Pulp Stem Cells (DPSCs) were cultured on titanium in order to analyze their biocompatibility, cell adhesion, and osteoconductive properties. All tested specimens were biocompatible, due to the time-dependent increase of DPSC proliferation paralleled by the decrease of LDH released. Furthermore, data highlighted that the open cell form with interconnected pores of titanium A, resembling the inner structure of the native bone, allows cells to better adhere inside the specimen, being proteins related to cell adherence highly expressed. Likewise, titanium A displays more suitable osteoconductive properties, being the profile of osteogenic markers improved compared to titanium B and C. The present work has demonstrated that the inner design and post-production treatments on titanium surfaces have a dynamic influence on DPSC behavior toward adhesion and osteogenic commitment.

Thermo- and pH-Responsive Gelatin/Polyphenolic Tannin/Graphene Oxide Hydrogels for Efficient Methylene Blue Delivery

Gelatin (GE), amino-functionalized polyphenolic tannin derivative (TN), and graphene oxide (GO) were associated to yield thermo- and pH-responsive hydrogels for the first time. Durable hydrogel assemblies for drug delivery purposes were developed using the photosensitizer methylene blue (MB) as a drug model. The cooling GE/TN blends provide brittle physical assemblies. To overcome this disadvantage, different GO contents (between 0.31% and 1.02% wt/wt) were added to the GE/TN blend at 89.7/10.3 wt/wt. FTIR and RAMAN spectroscopy analyses characterized the materials, indicating GO presence in the hydrogels. Incorporation studies revealed a total MB (0.50 mg/mL) incorporation into the GE/TN-GO hydrogel matrices. Additionally, the proposed systems present a mechanical behavior similar to gel. The GO presence in the hydrogel matrices increased the elastic modulus from 516 to 1650 Pa. SEM revealed that hydrogels containing MB present higher porosity with interconnected pores. Dissolution and swelling degree studies revealed less stability of the GE/TN-GO-MB hydrogels in SGF medium (pH 1.2) than SIF (pH 6.8). The degradation increased in SIF with the GO content, making the polymeric matrices more hydrophilic. MB release studies revealed a process controlled by Fickian diffusion. Our results point out the pH-responsible behavior of mechanically reinforced GE/TN-GO-MB hydrogels for drug delivery systems purposes.

Type II Collagen Sponges Facilitate Tendon Stem/Progenitor Cells to Adopt More Chondrogenic Phenotypes and Promote the Regeneration of Fibrocartilage-Like Tissues in a Rabbit Partial Patellectomy Model

ObjectiveFibrocartilage transition zone (FC) is difficult to regenerate after surgical re-attachment of tendon to bone. Here, we investigated whether type II collagen-sponges (CII-sponges) facilitated tendon stem/progenitor cells (TSPCs) to adopt chondrogenic phenotypes and further observed if this material could increase the FC areas in bone-tendon junction (BTJ) injury model.MethodsCII-sponges were made as we previously described. The appearance and pore structure of CII-sponges were photographed by camera and microscopies. The viability, proliferation, and differentiation of TSPCs were examined by LIVE/DEAD assay, alamarBlue, and PKH67 in vitro tracking. Subsequently, TSPCs were seeded in CII-sponges, Matrigel or monolayer, and induced under chondrogenic medium for 7 or 14 days before being harvested for qPCR or being transplanted into nude mice to examine the chondrogenesis of TSPCs. Lastly, partial patellectomy (PP) was applied to establish the BTJ injury model. CII-sponges were interposed between the patellar fragment and tendon, and histological examination was used to assess the FC regeneration at BTJ after surgery at 8 weeks.ResultsCII-sponges were like sponges with interconnected pores. TSPCs could adhere, proliferate, and differentiate in this CII-sponge up to 14 days at least. Both qPCR and immunostaining data showed that compared with TSPCs cultured in monolayer or Matrigel, cells in CII-sponges group adopted more chondrogenic phenotypes with an overall increase of chondrocyte-related genes and proteins. Furthermore, in PP injured model, much more new formed cartilage-like tissues could be observed in CII-sponges group, evidenced by a large amount of positive proteoglycan expression and typical oval or round chondrocytes in this area.ConclusionOur study showed that CII-sponges facilitated the TSPCs to differentiate toward chondrocytes and increased the area of FCs, which suggests that CII-sponges are meaningful for the reconstruction of FC at bone tendon junction. However, the link between the two phenomena requires further research and validation.

MOLDABLE AND MACHINABLE POROUS CARBON STRUCTURES OBTAINED FROM WHEY

A new porous carbon structures made by sintering whey powder in a mold at 150ºC and subsequent carbonization and/or activation are described. These porous carbon structures represent a step beyond simple activated carbon monoliths, as they can take the shape of any mold and machined if necessary. These carbon materials have a porosity that can reach up 70%, made up of a system of hierarchical and interconnected pores, which gives them a high permeability. Furthermore, they have a much higher mechanical strength than other porous carbon monoliths. Key Words: carbon structures, monoliths, porous carbon, activated carbon, machining, sintering, whey, whey powder.