scholarly journals Effect of some Factors on the Proteolytic Activities of Bromelain, Cichorium and Papain Extracts

2019 ◽  
Vol 6 (1) ◽  
pp. 1-7
Keyword(s):  
Proteolytic Activities

Comparison of proteolytic activities of the enzyme complex from mammalian pancreas and pancreatin

2000 ◽  
Vol 36 (4) ◽  
pp. 363-367 ◽  
Author(s):  
A. V. Berdutina ◽  
A. D. Neklyudov ◽  
A. I. Ivankin ◽  
B. S. Karpo ◽  
S. I. Mitaleva
Keyword(s):  
Enzyme Complex ◽  
Proteolytic Activities

scholarly journals A Milk Foodomics Investigation into the Effect of Pseudomonas fluorescens Growth under Cold Chain Conditions

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1173
Author(s):  
Paolo Bellassi ◽  
Gabriele Rocchetti ◽  
Lorenzo Morelli ◽  
Biancamaria Senizza ◽  
Luigi Lucini ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Degradation Products ◽  
Storage Conditions ◽  
Cold Chain ◽  
Lower Sensitivity ◽  
Multivariate Statistical ◽  
Proteolytic Activities ◽  
Quality Of Milk ◽  
Potential Tool

Pseudomonas fluorescens is a psychrotrophic species associated with milk spoilage because of its lipolytic and proteolytic activities. Consequently, monitoring P. fluorescens or its antecedent activity in milk is critical to preventing quality defects of the product and minimizing food waste. Therefore, in this study, untargeted metabolomics and peptidomics were used to identify the changes in milk related to P. fluorescens activity by simulating the low-temperature conditions usually found in milk during the cold chain. Both unsupervised and supervised multivariate statistical approaches showed a clear effect caused by the P. fluorescens inoculation on milk samples. Our results showed that the levels of phosphatidylglycerophosphates and glycerophospholipids were directly related to the level of contamination. In addition, our metabolomic approach allowed us to detect lipid and protein degradation products that were directly correlated with the degradative metabolism of P. fluorescens. Peptidomics corroborated the proteolytic propensity of P. fluorescens-contaminated milk, but with lower sensitivity. The results obtained from this study provide insights into the alterations related to P. fluorescens 39 contamination, both pre and post heat treatment. This approach could represent a potential tool to retrospectively understand the actual quality of milk under cold chain storage conditions, either before or after heat treatments.

Persistence of candiduria in ICU catheterized patients is not linked to adherence and proteolytic activities of Candida strains

2004 ◽  
Vol 30 (5) ◽  
pp. 972-975 ◽  
Author(s):  
Halis Akalın ◽  
Beyza Ener ◽  
Ferda Kahveci ◽  
Sevim Akçağlar ◽  
Şaban Gürcan ◽  
...  
Keyword(s):  
Proteolytic Activities

scholarly journals The proteolytic activities of the pancreatic juice

1903 ◽  
Vol 30 (1) ◽  
pp. 61-83 ◽  
Author(s):  
W. M. Bayliss ◽  
E. H. Starling
Keyword(s):  
Pancreatic Juice ◽  
Proteolytic Activities

Antioxidants, Free Radicals, Storage Proteins, Puroindolines, and Proteolytic Activities in Bread Wheat (Triticum aestivum) Seeds during Accelerated Aging

2004 ◽  
Vol 52 (13) ◽  
pp. 4274-4281 ◽  
Author(s):  
Lucia Calucci ◽  
Antonella Capocchi ◽  
Luciano Galleschi ◽  
Silvia Ghiringhelli ◽  
Calogero Pinzino ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Free Radicals ◽  
Bread Wheat ◽  
Storage Proteins ◽  
Accelerated Aging ◽  
Proteolytic Activities

Effect of neurotoxic metal ions on the proteolytic activities of the 20S proteasome from bovine brain

2002 ◽  
Vol 7 (7-8) ◽  
pp. 750-756 ◽  
Author(s):  
M. Amici ◽  
K. Forti ◽  
C. Nobili ◽  
G. Lupidi ◽  
M. Angeletti ◽  
...  
Keyword(s):  
Metal Ions ◽  
Bovine Brain ◽  
20S Proteasome ◽  
Proteolytic Activities

A novel milk-clotting enzyme from Aspergillus oryzae and A. luchuensis is an aspartic endopeptidase PepE presumed to be a vacuolar enzyme

2022 ◽  
Author(s):  
Yoko Takyu ◽  
Taro Asamura ◽  
Ayako Okamoto ◽  
Hiroshi Maeda ◽  
Michio Takeuchi ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Milk Clotting ◽  
Milk Clotting Enzyme ◽  
Proteolytic Activities ◽  
Homologous Enzyme ◽  
Traditional Fermentation ◽  
Enzyme Source ◽  
Cheese Production ◽  
Milk Clotting Activity ◽  
Aspartic Endopeptidase

Abstract Aspergillus oryzae RIB40 has 11 aspartic endopeptidase genes. We searched for milk-clotting enzymes based on the homology of the deduced amino acid sequence with chymosins. As a result, we identified a milk-clotting enzyme in A. oryzae. We expected other Aspergillus species to have a homologous enzyme with milk-clotting activity, and we found the most homologous aspartic endopeptidase from A. luchuensis had milk-clotting activity. Surprisingly, two enzymes were considered as vacuole enzymes according to a study on A. niger proteases. The two enzymes from A. oryzae and A. luchuensis cleaved a peptide between the 105Phe-106Met bond in κ-casein, similar to chymosin. Although both enzymes showed proteolytic activity using casein as a substrate, the optimum pH values for milk-clotting and proteolytic activities were different. Furthermore, the substrate specificities were highly restricted. Therefore, we expected that the Japanese traditional fermentation agent, koji, could be used as an enzyme source for cheese production.

Sign in / Sign up

Export Citation Format

Share Document