Cellulolytic activity of Trichoderma reesei and Bacillus subtilis against the plant pathogen Pythium debaryanum

Ahmed Salem; Hany Abdelrahman

doi:10.21608/jenvbs.2021.71242.1132

Overexpression of an exotic thermotolerant β-glucosidase in trichoderma reesei and its significant increase in cellulolytic activity and saccharification of barley straw

Microbial Cell Factories ◽

10.1186/1475-2859-11-63 ◽

2012 ◽

Vol 11 (1) ◽

pp. 63 ◽

Cited By ~ 56

Author(s):

Mehdi Dashtban ◽

Wensheng Qin

Keyword(s):

Trichoderma Reesei ◽

Barley Straw ◽

Cellulolytic Activity

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Antagonistic effects of volatiles generated by Bacillus subtilis on spore germination and hyphal growth of the plant pathogen, Botrytis cinerea

Biotechnology Letters ◽

10.1007/s10529-007-9626-9 ◽

2007 ◽

Vol 30 (5) ◽

pp. 919-923 ◽

Cited By ~ 68

Author(s):

Hua Chen ◽

Xiang Xiao ◽

Jun Wang ◽

Lijun Wu ◽

Zhiming Zheng ◽

...

Keyword(s):

Bacillus Subtilis ◽

Botrytis Cinerea ◽

Plant Pathogen ◽

Spore Germination ◽

Hyphal Growth ◽

Antagonistic Effects

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The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from Trichoderma reesei

Enzyme and Microbial Technology ◽

10.1016/s0141-0229(02)00235-1 ◽

2003 ◽

Vol 32 (1) ◽

pp. 35-40 ◽

Cited By ~ 27

Author(s):

M.A Lemos ◽

J.A Teixeira ◽

M.R.M Domingues ◽

M Mota ◽

F.M Gama

Keyword(s):

Trichoderma Reesei ◽

Cellulolytic Activity ◽

Cellobiohydrolase I ◽

Binding Domains ◽

Cellulose Binding

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PRODUKSI KITOOLIGOSAKARIDA MENGGUNAKAN SELULASE DARI Trichoderma reesei DAN BIOAKTIVITASNYA SEBAGAI ANTIBAKTERI

Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan ◽

10.15578/jpbkp.v4i2.442 ◽

2009 ◽

Vol 4 (2) ◽

pp. 105

Author(s):

Yusro Nuri Fawzya ◽

Manuntun Yohanes Sihotang ◽

Syarmalina Syarmalina ◽

Asri Pratitis

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Bacillus Subtilis ◽

Trichoderma Reesei

Penelitian ini merupakan sebagian dari rangkaian penelitian mengenai eksplorasi enzimenzim kitinolitik mikroba dan aplikasinya untuk pembuatan oligomer kitosan (kitooligosakarida) serta uji bioaktivitasnya. Tujuan penelitian ini adalah untuk menguji kemampuan antibakteri kitooligosakarida yang diproduksi dari kitosan dengan menggunakan enzim selulase non spesifik komersial dari Trichoderma reesei. Enzim ini ditentukan terlebih dahulu suhu dan pH optimumnya sebagai kitosanase, kemudian digunakan untuk menghidrolisis kitosan dengan konsentrasi 10, 13, dan 15 U/g kitosan, masing-masing selama 1, 2, dan 3 jam. Kitooligosakarida yang dihasilkan kemudian diidentifikasi dengan menggunakan khromatografi lapis tipis, dan diuji bioaktivitasnya sebagai antibakteri. Kitooligosakarida terpilih berdasarkan aktivitas antibakterinya kemudian diproduksi lagi untuk penentuan viskositas dan bobot molekulnya. Hasil penelitian menunjukkan bahwa enzim selulase ini bekerja optimal sebagai enzim kitosanase pada suhu 60ºC dan pH 6. Identifikasi dengan menggunakan khromatografi lapis tipis menunjukkan bahwa kitooligosakarida yang dihasilkan dari semua perlakuan mengandung dimer, trimer, tetramer, dan pentamer. Kitooligosakarida tersebut mampu menghambat pertumbuhan bakteri Staphylococcus aureus sampai dengan 84%, Salmonella typhosa sampai dengan 26%, Bacillus subtilis sampai dengan 15%, dan Escherichia coli sampai dengan 5,6%. Kitooligosakarida yang dihasilkan dari hidrolisis menggunakan enzim ini pada 10 U/g kitosan selama 1 jam memiliki viskositas 2,15 cPs dan bobot molekul 323,76 Da.

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Effect of C/N ratio and cellulose type on the cellulolytic activity of free and immobilized Trichoderma reesei

Immobilized Cells - Basics and Applications, Proceedings of an International Symposium organized under auspices of The Working Party on Applied Biocatalysis of the European Federation of Biotechnology Noordwijkerhout - Progress in Biotechnology ◽

10.1016/s0921-0423(96)80020-7 ◽

1996 ◽

pp. 136-139

Author(s):

V. Jirku

Keyword(s):

Trichoderma Reesei ◽

Cellulolytic Activity

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Enhancement of biotransformation of ginsenosides in white ginseng roots by aerobic co-cultivation of Bacillus subtilis and Trichoderma reesei

Applied Microbiology and Biotechnology ◽

10.1007/s00253-021-11631-1 ◽

2021 ◽

Author(s):

Guo Xie ◽

Bian-Qin Guo ◽

Xiao-Min Li ◽

Shuai Liu ◽

Hong-Xia Liu ◽

...

Keyword(s):

Bacillus Subtilis ◽

Trichoderma Reesei ◽

White Ginseng

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Complete Genome Sequence of Bacillus subtilis BSn5, an Endophytic Bacterium of Amorphophallus konjac with Antimicrobial Activity for the Plant Pathogen Erwinia carotovora subsp. carotovora

Journal of Bacteriology ◽

10.1128/jb.00129-11 ◽

2011 ◽

Vol 193 (8) ◽

pp. 2070-2071 ◽

Cited By ~ 65

Author(s):

Y. Deng ◽

Y. Zhu ◽

P. Wang ◽

L. Zhu ◽

J. Zheng ◽

...

Keyword(s):

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Genome Sequence ◽

Plant Pathogen ◽

Complete Genome Sequence ◽

Complete Genome ◽

Erwinia Carotovora ◽

Endophytic Bacterium ◽

Amorphophallus Konjac

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Literature study of production dry cellulase from Trichoderma reesei, Aspergillus niger, and Bacillus subtilis

IOP Conference Series Materials Science and Engineering ◽

10.1088/1757-899x/1073/1/012009 ◽

2021 ◽

Vol 1073 (1) ◽

pp. 012009

Author(s):

Y Maryanty ◽

D R Wulan ◽

M D Hidayati ◽

D A Rizal ◽

F Aliffandri

Keyword(s):

Bacillus Subtilis ◽

Aspergillus Niger ◽

Trichoderma Reesei ◽

Literature Study

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Hemicellulase Activity of Aerobic Fungal Cellulases

Holzforschung ◽

10.1515/hf.2000.084 ◽

2000 ◽

Vol 54 (5) ◽

pp. 497-500 ◽

Cited By ~ 9

Author(s):

M. Lawoko ◽

A. Nutt ◽

H. Henriksson ◽

G. Gellerstedt ◽

G. Henriksson

Keyword(s):

Trichoderma Reesei ◽

Phanerochaete Chrysosporium ◽

Biological Function ◽

Xylanase Activity ◽

Citrus Fruit ◽

Cellulolytic Activity ◽

Birchwood Xylan ◽

Mannanase Activity ◽

Locust Bean

Summary Cellulases isolated from Trichoderma reesei and Phanerochaete chrysosporium were screened for hemicellulolytic, pectinolytic and cellulolytic activity using locust bean mannan, birchwood xylan, citrus fruit pectin and carboxymethylated cellulose (CMC) as substrates. The purpose of this work was to choose appropriate enzymes to include in a “miniature cellulase system” with minimal hemicellulase activity for the preparation of lignin-carbohydrate complexes (LCCs). The endoglucanases showed CMC activity whereas activity towards the substrate was not detected for the CBHs. Xylanase activity was observed for EG I and EG 38 whereas mannanase activity was observed for EG 44. None of the enzymes degraded pectin. The results suggest that CBH I, CBH II, CBH 58, EG II and EG III are good candidates for the effective preparation of LCCs. The possible biological function for the hemicellulolytic activity of cellulases is discussed.

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