scholarly journals Effects of Steganotaenia Araliacae Root Extract on Contractile Function of Isolated Rat Ileum

2021 ◽  
Vol 3 (2) ◽  
pp. 32-41
Author(s):  
Pharaoh Hamambulu ◽  
◽  
Fastone Mathew-Goma ◽  
Newton Simfukwe ◽  
Lukubi Lwiindi ◽  
...  
Keyword(s):  
Dose Response ◽  
Contractile Function ◽  
Contractile Force ◽  
Tissue Response ◽  
Root Extract ◽  
Organ Bath ◽  
Rat Ileum ◽  
Uterine Muscle ◽  
High Doses ◽  
Isolated Rat

Background: Various parts of a small tree, Steganotaenia araliacaeare used as medicine in local traditional settings in Zambia to initiate and augment parturition although very little is documented about its physiological and pharmacological effects. Steganotaenia araliacaecold extract has been observed to cause contractions of uterus in rats but its effect on non-uterine muscle is unclear. The aim of this study was to establish the contractile effect of Steganotaenia araliacaecold extract on isolated rat ileum smooth muscle.Method: Animals were sacrificed by cervical dislocation. Abdominal incisions were made to expose and dissect three ileum segments from each rat. The ileum segments were immediately transferred and mounted in the organ bath containing Tyrode solution. The contractile effects of acetylcholine (a reference agonist) and Steganotaenia araliacaecold extract on ileum segments were investigated starting with the least effective doses, thereafter doubling the doses until maximal tissue response was observed. Antagonists that include atropine, indomethacin, mepyramine, ondansetron and nifedipine in the presence and absence of Steganotaenia araliacaecold extract were also investigated to establish the mechanism of actionResults: Steganotaenia araliacaecold extract increased the contractile force of isolated rat ileum in a dose-response manner but had no significant effects on the frequency of the spontaneous contractions. Pre-treating the tissue with atropine, indomethacin, mepyramine or ondansetron did not inhibit the contractile force of Steganotaenia araliacaecold extract, while pre-treating the tissue with nifedipine inhibited its contractile force by 100% (p<0.05)Conclusion: The cold root extract of Steganotaenia araliacae induced contractions on isolated rat ileum smooth muscle in a dose response manner by probable activation of calcium channels. It is possible that SAEᶜif used in high doses may cause severe abdominal cramps an effect that needs to be noted as it is being used in parturition.

scholarly journals High dilutions of homeopathic remedies induce relaxation of rat aorta precontracted with Noradrenalin

2021 ◽  
Vol 12 (43) ◽  
pp. 44-51
Author(s):  
Indrani Chakraborty ◽  
Nirmal Chandra Sukul ◽  
Rungrapa Mesripong ◽  
Nattaya Chaothanaphat ◽  
Prasan Dhummaupakorn ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Rat Aorta ◽  
Organ Bath ◽  
Rat Ileum ◽  
Treatment Of Hypertension ◽  
High Dilutions ◽  
Homeopathic Remedies ◽  
Homeopathic Drugs ◽  
Isolated Rat ◽  
Bathing Fluid

BACKGROUND Homeopathic potencies have been reported to produce alteration of contraction in isolated rat ileum in an organ bath. Potentized homeopathic drugs like Lycopus V and Aurum met are used for the treatment of hypertension. AIM The purpose of this study is to see whether Lycopus V 30 CH and Aurum met 30 CH could produce relaxation of isolated rat aorta in the organ bath. METHODS The aorta of rats were dissected out, placed in Krebs-Henseleit solution, cleared of connective tissue and endothelium and cut into 2-2.5 mm long rings. The rings were fixed in organ baths with the upper end connected by a string to an isometric transducer which was finally attached through a data acquisitation equipment to a computer. Aurum met 30 CH Lycopus V 30 CH, and their medium 90% ethanol were added separately to the bathing fluid containing the aorta rings which were precontracted with noradrenalin (NA). RESULTS Both the drugs produced significant relaxation of the aorta (p

scholarly journals Relaxant activity of aqueous and ethanol extracts of parsley (Petroselinum crispum (Mill.) Nym. ex A.W. Hill, Apiaceae) on isolated ileum of rat

2010 ◽  
Vol 63 (7-8) ◽  
pp. 475-478 ◽  
Author(s):  
Suzana Brankovic ◽  
Dusanka Kitic ◽  
Mirjana Radenkovic ◽  
Vesna Ivetic ◽  
Slavimir Veljkovic ◽  
...  
Keyword(s):  
Aqueous Ethanol ◽  
Ethanol Extract ◽  
Albino Rats ◽  
Petroselinum Crispum ◽  
Organ Bath ◽  
Dependent Manner ◽  
Response Curves ◽  
Rat Ileum ◽  
Ethanol Extracts ◽  
Isolated Rat

Introduction. Parsley (Petroselinum crispum) is used in the traditional herbal medicine to treat intestinal disorders. The aim of this study was to examine the effect of aqueous and ethanol extracts of parsley on spontaneous and acetylcholine induced contractions on isolated rat ileum. Material and methods. Wistar albino rats (250-300g) were used in this study. The ileum portions were isolated out and cleaned off mesenteries. Preparations 2 cm long were mounted in 20 ml tissue baths containing Tyrode's solution maintained at 37?C and aerated with a mixture of 5% carbon dioxide in oxygen. In the first part of experiments, contractile responses to the aqueous (ethanol) extracts of parsley were recorded. In the second part, increasing concentrations of acetylcholine were added to the organ bath for a full concentration response curve and then concentration response curves were obtained after adding the aqueous (ethanol) extracts of parsley. Results and discussion. Our results showed that aqueous (62.22?7.15%) and ethanol (79.16?9.34%) extracts of parsley in dose dependent manner decreased the tonus of spontaneous contractions of isolated rat ileum. The aqueous (32.16?2.75%) and ethanol (53.96?4.86%) extracts of parsley reduced the acetylcholine induced contraction, the reduction was greater with ethanol extract than with the aqueous one. Conclusion. It can be concluded that the aqueous and ethanol extracts of parsley exert antispasmodic activity on rat ileum. The relaxant effect of ethanol extract was better comparing to aqueous extract of parsley.

Inhibitory effect of GnRH on isolated rat uterine muscle contractility

Life Sciences ◽  
1988 ◽  
Vol 42 (21) ◽  
pp. 2055-2062 ◽  
Author(s):  
Yara S. Medeiros ◽  
João B. Calixto ◽  
Gustavo Ballejo
Keyword(s):  
Inhibitory Effect ◽  
Muscle Contractility ◽  
Uterine Muscle ◽  
Isolated Rat

scholarly journals Dysfunction of muscle contraction with impaired intracellular Ca2+ handling in skeletal muscle and the effect of exercise training in male db/db mice

2019 ◽  
Vol 126 (1) ◽  
pp. 170-182 ◽  
Author(s):  
Hiroaki Eshima ◽  
Yoshifumi Tamura ◽  
Saori Kakehi ◽  
Kyoko Nakamura ◽  
Nagomi Kurebayashi ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Skeletal Muscle ◽  
Exercise Training ◽  
Muscle Contraction ◽  
Contractile Function ◽  
Contractile Force ◽  
Contractile Dysfunction ◽  
Muscle Contractile ◽  
Type 2 Diabetic

Type 2 diabetes is characterized by reduced contractile force production and increased fatigability of skeletal muscle. While the maintenance of Ca2+ homeostasis during muscle contraction is a requisite for optimal contractile function, the mechanisms underlying muscle contractile dysfunction in type 2 diabetes are unclear. Here, we investigated skeletal muscle contractile force and Ca2+ flux during contraction and pharmacological stimulation in type 2 diabetic model mice ( db/db mice). Furthermore, we investigated the effect of treadmill exercise training on muscle contractile function. In male db/db mice, muscle contractile force and peak Ca2+ levels were both lower during tetanic stimulation of the fast-twitch muscles, while Ca2+ accumulation was higher after stimulation compared with control mice. While 6 wk of exercise training did not improve glucose tolerance, exercise did improve muscle contractile dysfunction, peak Ca2+ levels, and Ca2+ accumulation following stimulation in male db/db mice. These data suggest that dysfunctional Ca2+ flux may contribute to skeletal muscle contractile dysfunction in type 2 diabetes and that exercise training may be a promising therapeutic approach for dysfunctional skeletal muscle contraction. NEW & NOTEWORTHY The purpose of this study was to examine muscle contractile function and Ca2+ regulation as well as the effect of exercise training in skeletal muscle in obese diabetic mice ( db/db). We observed impairment of muscle contractile force and Ca2+ regulation in a male type 2 diabetic animal model. These dysfunctions in muscle were improved by 6 wk of exercise training.

Biochemical basis of heart function: 1. relation of catecholamine stores and contractile force in an isolated rat heart

1971 ◽  
Vol 5 (3) ◽  
pp. 376-382 ◽  
Author(s):  
N. S. Dhalla ◽  
K. J. R. Naidu ◽  
B. Bhagat ◽  
K. Cristensen
Keyword(s):  
Rat Heart ◽  
Heart Function ◽  
Contractile Force ◽  
Isolated Rat Heart ◽  
Biochemical Basis ◽  
Isolated Rat

Effects of KATP Channel Openers, P-1075, Pinacidil, and Diazoxide, on Energetics and Contractile Function in Isolated Rat Hearts

2002 ◽  
Vol 34 (4) ◽  
pp. 427-440 ◽  
Author(s):  
Olga Jilkina ◽  
Bozena Kuzio ◽  
Gary J. Grover ◽  
Valery V. Kupriyanov
Keyword(s):  
Katp Channel ◽  
Contractile Function ◽  
Rat Hearts ◽  
Isolated Rat ◽  
Katp Channel Openers

scholarly journals Treatment of Stress Urinary Incontinence by Cinnamaldehyde, the Major Constituent of the Chinese Medicinal HerbRamulus Cinnamomi

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Yung-Hsiang Chen ◽  
Yu-Ning Lin ◽  
Wen-Chi Chen ◽  
Wen-Tsong Hsieh ◽  
Huey-Yi Chen
Keyword(s):  
Nitric Oxide ◽  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Therapeutic Potential ◽  
Contractile Force ◽  
Survival Motor Neuron ◽  
Raw 264.7 Cells ◽  
Major Constituent ◽  
No Production ◽  
Organ Bath

Stress urinary incontinence (SUI) is a common disorder in middle-aged women and the elderly population. Although surgical treatment of SUI has progressed, pharmacological therapies remain unelucidated. We screened potential herbal medicines against SUI with anex vivoorgan bath assay.Ramulus Cinnamomiand its major constituent cinnamaldehyde cause a high contractile force of the urethra and a low contractile force of blood vessels. Cinnamaldehyde dose-dependently reduced lipopolysaccharide-induced nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in RAW 264.7 cells. In the vaginal distension- (VD-) induced SUI model in mice, cinnamaldehyde significantly reversed the VD-induced SUI physical signs and reduced blood pressure. Cinnamaldehyde may offer therapeutic potential against SUI without the possible side effect of hypertension. The modulation of several SUI-related proteins including myosin, iNOS, survival motor neuron (SMN) protein, and superoxide dismutase 3 (SOD3) may play some crucial roles in the therapeutic approach against SUI. This information may offer clues to the pathogenesis of SUI and open additional avenues for potential therapy strategies.

Improvement in ischemic myocardial blood flow following a new calcium antagonist

1980 ◽  
Vol 239 (2) ◽  
pp. H163-H171 ◽  
Author(s):  
S. R. Jolly ◽  
G. J. Gross
Keyword(s):  
Blood Flow ◽  
Perfusion Pressure ◽  
Aortic Pressure ◽  
Contractile Force ◽  
Tissue Blood Flow ◽  
Coronary Pressure ◽  
Ischemic Tissue ◽  
High Doses ◽  
Mean Aortic Pressure ◽  
Aortic Perfusion

The effect of FR 7534, a new dihydropyridine CA2+ antagonist, nitroglycerin, and dipyridamole have been compared on coronary collateral function in pentobarbital-anesthetized open-chest dogs following acute ligation of the left anterior descending coronary artery. Measurements of tissue blood flow using radioactive microspheres were made 60 min postligation after low and high doses of each drug and at the higher dose with methoxamine added to to return mean aortic pressure. FR 7534 treatment increased subepicardial, subendocardial, and transmural tissue flow by 100% in the central ischemic zone when perfusion pressure was controlled by methoxamine. Nitroglycerin also increased ischemic tissue flow, but to a lesser degree, 18% transmurally. Dipyridamole produced no significant change. Tissue flow in normal myocardium was similarly increased by FR 7534 and dipyridamole but slightly reduced by nitroglycerin. FR 7534 and nitroglycerin also increased retrograde coronary pressure when aortic presure was maintained constant. FR 7534, but not nitroglycerin, increased ischemic contractile force. In this model, FR 7534 may produce greater effects than nitroglycerin in increasing blood supply to ischemic myocardium delivered by endogenous collaterals especially when aortic perfusion pressure was controlled.

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