High dilutions of homeopathic remedies induce relaxation of rat aorta precontracted with Noradrenalin

BACKGROUND Homeopathic potencies have been reported to produce alteration of contraction in isolated rat ileum in an organ bath. Potentized homeopathic drugs like Lycopus V and Aurum met are used for the treatment of hypertension. AIM The purpose of this study is to see whether Lycopus V 30 CH and Aurum met 30 CH could produce relaxation of isolated rat aorta in the organ bath. METHODS The aorta of rats were dissected out, placed in Krebs-Henseleit solution, cleared of connective tissue and endothelium and cut into 2-2.5 mm long rings. The rings were fixed in organ baths with the upper end connected by a string to an isometric transducer which was finally attached through a data acquisitation equipment to a computer. Aurum met 30 CH Lycopus V 30 CH, and their medium 90% ethanol were added separately to the bathing fluid containing the aorta rings which were precontracted with noradrenalin (NA). RESULTS Both the drugs produced significant relaxation of the aorta (p

Download Full-text

Effects of Steganotaenia Araliacae Root Extract on Contractile Function of Isolated Rat Ileum

Journal of Preventive and Rehabilitative Medicine ◽

10.21617/jprm2021.328issn ◽

2021 ◽

Vol 3 (2) ◽

pp. 32-41

Author(s):

Pharaoh Hamambulu ◽

◽

Fastone Mathew-Goma ◽

Newton Simfukwe ◽

Lukubi Lwiindi ◽

...

Keyword(s):

Dose Response ◽

Contractile Function ◽

Contractile Force ◽

Tissue Response ◽

Root Extract ◽

Organ Bath ◽

Rat Ileum ◽

Uterine Muscle ◽

High Doses ◽

Isolated Rat

Background: Various parts of a small tree, Steganotaenia araliacaeare used as medicine in local traditional settings in Zambia to initiate and augment parturition although very little is documented about its physiological and pharmacological effects. Steganotaenia araliacaecold extract has been observed to cause contractions of uterus in rats but its effect on non-uterine muscle is unclear. The aim of this study was to establish the contractile effect of Steganotaenia araliacaecold extract on isolated rat ileum smooth muscle.Method: Animals were sacrificed by cervical dislocation. Abdominal incisions were made to expose and dissect three ileum segments from each rat. The ileum segments were immediately transferred and mounted in the organ bath containing Tyrode solution. The contractile effects of acetylcholine (a reference agonist) and Steganotaenia araliacaecold extract on ileum segments were investigated starting with the least effective doses, thereafter doubling the doses until maximal tissue response was observed. Antagonists that include atropine, indomethacin, mepyramine, ondansetron and nifedipine in the presence and absence of Steganotaenia araliacaecold extract were also investigated to establish the mechanism of actionResults: Steganotaenia araliacaecold extract increased the contractile force of isolated rat ileum in a dose-response manner but had no significant effects on the frequency of the spontaneous contractions. Pre-treating the tissue with atropine, indomethacin, mepyramine or ondansetron did not inhibit the contractile force of Steganotaenia araliacaecold extract, while pre-treating the tissue with nifedipine inhibited its contractile force by 100% (p<0.05)Conclusion: The cold root extract of Steganotaenia araliacae induced contractions on isolated rat ileum smooth muscle in a dose response manner by probable activation of calcium channels. It is possible that SAEᶜif used in high doses may cause severe abdominal cramps an effect that needs to be noted as it is being used in parturition.

Download Full-text

Relaxant activity of aqueous and ethanol extracts of parsley (Petroselinum crispum (Mill.) Nym. ex A.W. Hill, Apiaceae) on isolated ileum of rat

Medicinski pregled ◽

10.2298/mpns1008475b ◽

2010 ◽

Vol 63 (7-8) ◽

pp. 475-478 ◽

Cited By ~ 5

Author(s):

Suzana Brankovic ◽

Dusanka Kitic ◽

Mirjana Radenkovic ◽

Vesna Ivetic ◽

Slavimir Veljkovic ◽

...

Keyword(s):

Aqueous Ethanol ◽

Ethanol Extract ◽

Albino Rats ◽

Petroselinum Crispum ◽

Organ Bath ◽

Dependent Manner ◽

Response Curves ◽

Rat Ileum ◽

Ethanol Extracts ◽

Isolated Rat

Introduction. Parsley (Petroselinum crispum) is used in the traditional herbal medicine to treat intestinal disorders. The aim of this study was to examine the effect of aqueous and ethanol extracts of parsley on spontaneous and acetylcholine induced contractions on isolated rat ileum. Material and methods. Wistar albino rats (250-300g) were used in this study. The ileum portions were isolated out and cleaned off mesenteries. Preparations 2 cm long were mounted in 20 ml tissue baths containing Tyrode's solution maintained at 37?C and aerated with a mixture of 5% carbon dioxide in oxygen. In the first part of experiments, contractile responses to the aqueous (ethanol) extracts of parsley were recorded. In the second part, increasing concentrations of acetylcholine were added to the organ bath for a full concentration response curve and then concentration response curves were obtained after adding the aqueous (ethanol) extracts of parsley. Results and discussion. Our results showed that aqueous (62.22?7.15%) and ethanol (79.16?9.34%) extracts of parsley in dose dependent manner decreased the tonus of spontaneous contractions of isolated rat ileum. The aqueous (32.16?2.75%) and ethanol (53.96?4.86%) extracts of parsley reduced the acetylcholine induced contraction, the reduction was greater with ethanol extract than with the aqueous one. Conclusion. It can be concluded that the aqueous and ethanol extracts of parsley exert antispasmodic activity on rat ileum. The relaxant effect of ethanol extract was better comparing to aqueous extract of parsley.

Download Full-text

Effects of liposome-entrapped adenosine in the isolated rat aorta

European Journal of Pharmacology ◽

10.1016/0014-2999(93)90040-o ◽

1993 ◽

Vol 250 (3) ◽

pp. 489-492 ◽

Cited By ~ 8

Author(s):

Eugen Brailoiu ◽

Dragomir N. Serban ◽

Sebastian Slatineanu ◽

Catalin M. Filipeanu ◽

Bogdan C. Petrescu ◽

...

Keyword(s):

Rat Aorta ◽

Isolated Rat

Download Full-text

Human plasma-derived material with clonidine displacing substance (CDS)-like properties contracts the isolated rat aorta

Journal of Autonomic Pharmacology ◽

10.1111/j.1474-8673.1991.tb00258.x ◽

1991 ◽

Vol 11 (6) ◽

pp. 343-351 ◽

Cited By ~ 15

Author(s):

Dennis Synetos ◽

Vangelis G. Manolopoulos ◽

Daphne Atlas ◽

Eva Pipili-Synetos

Keyword(s):

Human Plasma ◽

Rat Aorta ◽

Isolated Rat

Download Full-text

Acetylcholine-induced endothelium-dependent vascular smooth muscle relaxation in nitroglycerin-tolerant isolated rat aorta

Heart and Vessels ◽

10.1007/bf02058283 ◽

1991 ◽

Vol 6 (3) ◽

pp. 175-180 ◽

Cited By ~ 3

Author(s):

Atsushi Namiki ◽

Jo Aikawa ◽

Masao Moroi ◽

Kiyoshi Machii ◽

Nobuharu Akatsuka

Keyword(s):

Smooth Muscle ◽

Vascular Smooth Muscle ◽

Muscle Relaxation ◽

Rat Aorta ◽

Smooth Muscle Relaxation ◽

Vascular Smooth Muscle Relaxation ◽

Isolated Rat

Download Full-text

Levobupivacaine-induced contraction of isolated rat aorta is calcium dependent

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y11-046 ◽

2011 ◽

Vol 89 (7) ◽

pp. 467-476 ◽

Cited By ~ 22

Author(s):

Ji Seok Baik ◽

Ju-Tae Sohn ◽

Seong-Ho Ok ◽

Jae-Gak Kim ◽

Hui-Jin Sung ◽

...

Keyword(s):

Methylene Blue ◽

Smooth Muscle ◽

Calcium Influx ◽

Rat Aorta ◽

Isometric Tension ◽

Guanosine Monophosphate ◽

Response Curves ◽

Calcium Dependent ◽

Isolated Rat

Levobupivacaine is a long-acting local anesthetic that intrinsically produces vasoconstriction in isolated vessels. The goals of this study were to investigate the calcium-dependent mechanism underlying levobupivacaine-induced contraction of isolated rat aorta in vitro and to elucidate the pathway responsible for the endothelium-dependent attenuation of levobupivacaine-induced contraction. Isolated rat aortic rings were suspended to record isometric tension. Cumulative levobupivacaine concentration–response curves were generated in either the presence or absence of the antagonists verapamil, nifedipine, SKF-96365, 2-aminoethoxydiphenylborate, Gd3+, NW-nitro-l-arginine methyl ester (L-NAME), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), and methylene blue, either alone or in combination. Verapamil, nifedipine, SKF-96365, 2-aminoethoxydiphenylborate, low calcium concentrations, and calcium-free Krebs solution attenuated levobupivacaine-induced contraction. Gd3+ had no effect on levobupivacaine-induced contraction. Levobupivacaine increased intracellular calcium levels in vascular smooth muscle cells. L-NAME, ODQ, and methylene blue increased levobupivacaine-induced contraction in endothelium-intact aorta. SKF-96365 attenuated calcium-induced contraction in a previously calcium-free isotonic depolarizing solution containing 100 mmol/L KCl. Levobupivacaine-induced contraction of rat aortic smooth muscle is mediated primarily by calcium influx from the extracellular space mainly via voltage-operated calcium channels and, in part, by inositol 1,4,5-trisphosphate receptor-mediated release of calcium from the sarcoplasmic reticulum. The nitric oxide – cyclic guanosine monophosphate pathway is involved in the endothelium-dependent attenuation of levobupivacaine-induced contraction.

Download Full-text