Cell Surface and Aggregation Studies of Microbes from Anaerobic Systems

1987 ◽  
Vol 22 (2) ◽  
pp. 289-297 ◽  
Author(s):  
N. Kosaric ◽  
E.M. Mahoney ◽  
L.K. Varangu ◽  
W.L. Cairns
Keyword(s):  
Contact Angle ◽  
Anaerobic Digestion ◽  
Zeta Potential ◽  
Cell Surface ◽  
Surface Properties ◽  
Experimental Conditions ◽  
Cell Surface Properties ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Engineering Parameters

Abstract Studies were performed to evaluate cell surface properties of aggregated microbial granules from anaerobic digestion systems. Cell surface properties (hydrophobicity/hydrophilicity) were obtained by contact angle measurements in an aqueous phase. The cells’ charges were evaluated by measuring zeta potential and settlability was used as a measure of aggregating of cells under various experimental conditions. Flocculation tests have shown to be a good indicator of aggregation ability between cells. Aggregation can be modulated by changing those environmental and engineering parameters which can be measured by contact angle and zeta potential.

Estimation of the Surface Properties of Styrene-Acrylonitrile Random Copolymers from Contact Angle Measurements

1999 ◽  
Vol 217 (1) ◽  
pp. 94-106 ◽  
Author(s):  
Maria Helena Ventura Cabral Adão ◽  
Benilde Jesus Vieira Saramago ◽  
Anabela Catarino Fernandes
Keyword(s):  
Contact Angle ◽  
Surface Properties ◽  
Random Copolymers ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Styrene Acrylonitrile

Surface Properties of Whey Protein Gels

2019 ◽  
Vol 41 (6) ◽  
pp. 956-956
Author(s):  
Salvador Perez Huertas Salvador Perez Huertas ◽  
Konrad Terpi owski and Marta Tomczy ska Mleko Konrad Terpi owski and Marta Tomczy ska Mleko
Keyword(s):  
Contact Angle ◽  
Whey Protein ◽  
Surface Properties ◽  
Human Organism ◽  
New Methods ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Optical Profilometer ◽  
Protein Gels ◽  
Microscopic Techniques

Surface properties of whey protein gels are reviewed based on traditional microscopic techniques and new methods, as optical profilometer and contact angle measurements. Optical profilometer is an instrument allowing measurement of surface roughness and contact angle measurements to determine the surface wettability behavior (hydrophobicity/hydrophilicity) of the gels. Investigation of surface properties of whey protein gels is very important, as it can transform this product to a new level of application. It could be used as a matrix for an active ingredient release, material for tissue engineering, e.g. scaffolds, i.e. temporally structures biodegraded in the human organism.

Nonspecific Cell Surface Properties: Contact Angle of Water on Dried Cell Monolayers

1984 ◽  
Vol 13 (3) ◽  
pp. 211-227 ◽  
Author(s):  
J. L. Mege ◽  
C. Capo ◽  
A. M. Benoliel ◽  
C. Foa ◽  
P. Bongrand
Keyword(s):  
Contact Angle ◽  
Cell Surface ◽  
Surface Properties ◽  
Cell Surface Properties ◽  
Cell Monolayers

Surface characterization of glass fibers made from silicate waste: Zeta-potential and contact angle measurements

2004 ◽  
Vol 39 (2) ◽  
pp. 401-412 ◽  
Author(s):  
A. Bismarck ◽  
A. R. Boccaccini ◽  
E. Egia-Ajuriagojeaskoa ◽  
D. Hülsenberg ◽  
T. Leutbecher
Keyword(s):  
Contact Angle ◽  
Zeta Potential ◽  
Surface Characterization ◽  
Glass Fibers ◽  
Angle Measurements ◽  
Contact Angle Measurements

scholarly journals Bacterial Cell Surface Damage Due to Centrifugal Compaction

2011 ◽  
Vol 78 (1) ◽  
pp. 120-125 ◽  
Author(s):  
Brandon W. Peterson ◽  
Prashant K. Sharma ◽  
Henny C. van der Mei ◽  
Henk J. Busscher
Keyword(s):  
Zeta Potential ◽  
Cell Surface ◽  
Surface Properties ◽  
Bacterial Cell ◽  
Surface Damage ◽  
Deposition Rates ◽  
Content Type ◽  
Cell Surface Properties ◽  
Bacterial Cell Surface ◽  
Initial Deposition

ABSTRACTCentrifugal damage has been known to alter bacterial cell surface properties and interior structures, including DNA. Very few studies exist on bacterial damage caused by centrifugation because of the difficulty in relating centrifugation speed and container geometry to the damage caused. Here, we provide a simple, versatile method of analysis for describing the compaction of bacteria during centrifugation based on a proposed centrifugation coefficient,C. Values ofCcan be related to different bacterial cell surface properties. Changing the geometry of the centrifugation container or centrifugation speeds changed the value ofCsignificantly. Initial deposition rates ofStaphylococcus aureusATCC 12600 to a glass surface decayed exponentially from 4,217 to 1,478 cm−2s−1with increasingC, while the proportion of staphylococci with a zeta potential of around −15 mV decreased from 97 to 58%. These surface-sensitive parameters were used independently to derive a critical centrifugation coefficient (0.040), above which centrifugation was considered to impact the outcome of surface-sensitive experiments due to cell surface damage. The critical centrifugation coefficient could successfully predict staphylococcal cell surface damage, i.e., a significant change in initial deposition rate or zeta potential distribution, in 84% of all cases included here, whereas the centrifugation speed could predict damage in only 58% of all cases. Moreover, controlling the centrifugation coefficient within narrow limits over a series of experiments yielded 43% smaller standard deviations in initial staphylococcal deposition rates than with centrifugation at fixed speeds for replicate experiments.

Tuning the Surface Properties of Elastomers Using Hydrocarbon-Based Mechanically Assembled Monolayers

2001 ◽  
Vol 710 ◽  
Author(s):  
Kirill Efimenko ◽  
Jan Genzer
Keyword(s):  
Contact Angle ◽  
Fine Structure ◽  
Surface Structure ◽  
Surface Properties ◽  
Molecular Orientation ◽  
Angle Measurements ◽  
Static Contact ◽  
Contact Angle Measurements ◽  
Assembled Monolayers ◽  
Excellent Stability

ABSTRACTWe use static contact angle measurements and near-edge absorption fine structure to elucidate the surface structure and molecular orientation of hydrocarbon-based mechanically assembled monolayers (H-MAMs), structures formed by combination of assembly of alkyl moieties onto flexible elastomeric substrates and mechanical manipulation of the substrates. Specifically, we report that the organization of the grafted molecular array (“liquid”-like vs. solid-like) can be tailored by varying the degree of stretching of the elastomeric substrate. We also show that the H-MAM surfaces exhibit excellent stability.

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